Name: Enterobacter cloacae A-8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4363

Type strain:

Species: Enterobacter cloacae

Strain Designation: A-8

Culture col. no.: DSM 26481, ATCC 23355, CCUG 33777, CECT 5075, CIP 103550, WDCM 00082

Strain history: <- CECT <- CIP <- R. Mercier, bioMérieux, La Balme-les-Grottes, France <- ATCC <- Dept. Medical Microbiol, Stanford Univ.

NCBI tax ID(s): 550 (species)

SI-ID 38005

ATCC 23355, CCUG 33777, CIP 103550, CECT 5075, KCTC 2519, BCRC 11507, CCRC 11507, DSM 26481

Other strains from Enterobacter cloacae

Section

Enterobacter cloacae A-8 is a mesophilic, motile bacterium that has multiple antibiotic resistances.

antibiotic resistance
motile
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Enterobacter*
	Species	**Enterobacter cloacae**
	Full Scientific Name (LPSN)	Enterobacter cloacae (Jordan 1890) Hormaeche and Edwards 1960 (Approved Lists 1980)

	Synonym	"Cloaca* cloacae"*
	Synonym	"Bacillus* cloacae"*
	Synonym	"Bacterium* cloacae"*

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.976

[Ref.: #69480]	Motility	yes Confidence in %97.133

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18242]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #18242]

Culture medium growth

[Ref.: #18242]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18242]

Culture medium composition

expand / minimize

[Ref.: #18242]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #18242]

Culture medium growth

[Ref.: #18242]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18242]

Culture medium composition

expand / minimize

[Ref.: #18242]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #18242]

Culture medium growth

[Ref.: #18242]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18242]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #18242]			growth	28 °C
[Ref.: #18242]			growth	37 °C

[Ref.: #18242]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Reference

[Ref.: #18242]
Mueller-Hinton Agar
30
1
aerob
yes
Inhibition zone diameter in mm
8
resistant0
26
38-40
30
18
28
40
42
40
30
22-24
26
28
resistant0
resistant0
resistant0
36
36
16-18
30
36
8
18
30
22
22-24
38
resistant0
resistant0
34
resistant0
resistant0
resistant0
resistant0
32-34

[Ref.: #18242]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
6
resistant0
28-30
40
32
18
28
42
44-46
40-42
32
30-32
26
28
6
6-8
resistant0
40
40
16
32
36
resistant0
18-20
28-30
22
26
40-42
resistant0
resistant0
38-40
6-8
resistant0
resistant0
resistant0
34

Only first 15 entries are displayed. Click here to see all.

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.901

[Ref.: #18242]	Name of produced compound	cephalosporinase
[Ref.: #18242]	Name of produced compound	beta lactamase

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E, API ID32E
[Ref.: #68374]	cellobiose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-galacturonic acid ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-glucose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-rhamnose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E, API ID32E
[Ref.: #68374]	malonate ChEBI	+	assimilation	* from API ID32E
[Ref.: #68374]	maltose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	myo-inositol ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E, API ID32E
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	ribitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68374]	sucrose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	trehalose ChEBI	+	builds acid from	* from API ID32E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E, API ID32E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E, API ID32E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68374]	indole ChEBI	no	* from API ID32E, API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	-		* from API 20E
[Ref.: #68374]		indole ChEBI		-	* from API ID32E, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68374]	alpha-galactosidase	+	3.2.1.22	* from API ID32E
[Ref.: #68374]	alpha-glucosidase	-	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	-		* from API ID32E
[Ref.: #68374]	arginine dihydrolase	+	3.5.3.6	* from API ID32E, API 20E
[Ref.: #68374]	beta-galactosidase	+	3.2.1.23	* from API ID32E, API 20E
[Ref.: #68374]	beta-glucosidase	+	3.2.1.21	* from API ID32E
[Ref.: #68374]	beta-glucuronidase	-	3.2.1.31	* from API ID32E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68374]	L-aspartate arylamidase	-	3.4.11.21	* from API ID32E
[Ref.: #68374]	lipase	-		* from API ID32E
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E, API 20E
[Ref.: #68374]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API ID32E
[Ref.: #68374]	ornithine decarboxylase	+	4.1.1.17	* from API ID32E, API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68374]	urease	-	3.5.1.5	* from API ID32E, API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18242]	16764	+	+	-	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #51730]	1304	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	+	-	-	+	+	-	-	-	-	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18242]	Country	USA
[Ref.: #18242]	Country ISO 3 Code	USA
[Ref.: #18242]	Continent	North America
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence KF516238 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	1353
Soil Samples	615
Animal Samples	10132
Plant Samples	1234
Total Samples	13334

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18242]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Enterobacter cloacae subsp. cloacae strain ATCC 23355 16S ribosomal RNA gene, partial sequence	KF516238	1347	ENA	336306 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterobacter cloacae strain DSM 26481	550.2783	complete	PATRIC	550 tax ID	*
[Ref.: #66792]	Enterobacter cloacae DSM 26481	GCA_013375935	chromosome	GenBank	550 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Enterobacter cloacae strain DSM 26481 PATRIC: 550.2783
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterobacter cloacae strain DSM 26481 PATRIC: 550.2783
[Ref.: #69480]	gram-positive	no	97.822	no
[Ref.: #69480]	anaerobic	no	96.404	no
[Ref.: #69480]	halophile	no	84.237	no
[Ref.: #69480]	spore-forming	no	91.908	no
[Ref.: #69480]	glucose-util	yes	95.304	no
[Ref.: #69480]	thermophile	no	99.237	yes
[Ref.: #69480]	aerobic	yes	81.312	no
[Ref.: #69480]	motile	yes	89.15	no
[Ref.: #69480]	flagellated	no	64.522	no
[Ref.: #69480]	glucose-ferment	yes	93.998	yes

Availability in culture collections External links

[Ref.: #18242]

Culture collection no.

DSM 26481, ATCC 23355, CCUG 33777, CECT 5075, CIP 103550, WDCM 00082

[Ref.: #73854]

SI-ID 38005

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Pathogenicity	Antibacterial naphthoquinone derivatives targeting resistant strain Gram-negative bacteria in biofilms.	Novais JS, Moreira CS, Silva ACJA, Loureiro RS, Sa Figueiredo AM, Ferreira VF, Castro HC, da Rocha DR	Microb Pathog	10.1016/j.micpath.2018.03.024	2018	*
Pathogenicity	Searching for a potential antibacterial lead structure against bacterial biofilms among new naphthoquinone compounds.	Moreira CS, Silva AC, Novais JS, Sa Figueiredo AM, Ferreira VF, da Rocha DR, Castro HC	J Appl Microbiol	10.1111/jam.13369	2017	*
Pathogenicity	A high-throughput colorimetric-based bioassay method for determination of fosmidomycin in plasma and urine and application for pharmacokinetic study.	Cheoymang A, Na-Bangchang K	J Pharmacol Toxicol Methods	10.1016/j.vascn.2010.02.012	2010	*
Enzymology	Bioassay for determination of fosmidomycin in plasma and urine: application for pharmacokinetic dose optimisation.	Cheoymang A, Hudchinton D, Kioy D, Na-Bangchang K	J Microbiol Methods	10.1016/j.mimet.2006.11.018	2007	*
Pathogenicity	Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test.	White RL, Burgess DS, Manduru M, Bosso JA	Antimicrob Agents Chemother	10.1128/AAC.40.8.1914	1996	*
Enzymology	Localization of cephalosporinase in Enterobacter cloacae by immunocytochemical examination.	Ishii Y, Ichikawa M, Yamaguchi K, Takano K, Inoue M	J Antibiot (Tokyo)	10.7164/antibiotics.44.1088	1991	*
Enzymology	Novel method for studying the public health significance of macroinvertebrates occurring in potable water.	Levy RV, Cheetham RD, Davis J, Winer G, Hart FL	Appl Environ Microbiol	10.1128/aem.47.5.889-894.1984	1984	*
	Antibacterial activities of two potential peptides extracted from Polistes wattii Cameron, 1900 (Vespidae: Polistinae) wasp venom collected at Eastern Province, Saudi Arabia.	A Al-Shammery K, Hozzein WN	PLoS One	10.1371/journal.pone.0264035	2022	*
	First Report of Enterobacter Bulb Decay of Onions Caused by Enterobacter cloacae in New York.	Zaid AM, Bonasera JM, Beer SV	Plant Dis	10.1094/PDIS-05-11-0375	2011	*
	First Report of Enterobacter cloacae Causing Onion Bulb Rot in the Columbia Basin of Washington State.	Schroeder BK, du Toit LJ, Schwartz HF	Plant Dis	10.1094/PDIS-93-3-0323A	2009	*

References

#18242

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26481

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#51730 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33777

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68374

Automatically annotated from API ID32E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73854

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38005.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Enterobacter cloacae in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Enterobacter cloacae strain DSM 26481

Enterobacter cloacae strain DSM 26481

Change proposal

Change proposal