Nitrososphaera viennensis (DSM 26422, EN76, JCM 19564)

Name: Nitrososphaera viennensis (DSM 26422, EN76, JCM 19564)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24819

Type strain

Strain Designation EN76

Culture col. no. DSM 26422 EN76 JCM 19564

NCBI tax ID(s) 1034015

Special Collections DSMZ JCM

Links

Nitrososphaera viennensis EN76 is a thermophilic prokaryote that was isolated from garden soil.

thermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 401566

JCM 19564,DSM 26422

@ref 20215

Last LPSN update 2025-12-16 09:52:57

Domain Thermoproteati

Phylum Thermoproteota

Class Nitrososphaeria

Order Nitrososphaerales

Family Nitrososphaeraceae

Genus Nitrososphaera

Species Nitrososphaera viennensis

Full scientific name Nitrososphaera viennensis Stieglmeier et al. 2014

Synonyms (1)

"Candidatus Nitrososphaera viennensis"

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	97.6

Colony morphology

21344

Incubation period>14 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link
21344	NITROSOPUMILUS III MEDIUM (DSMZ Medium 1630c)		Medium recipe provided by DSMZ
21344	NITROSOSPHAERA MEDIUM (DSMZ Medium 1630)		Medium recipe at MediaDive

Culture temp

@ref	Growth	Type	Temperature (°C)
21344	positive	growth	40
67770	positive	growth	42

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate anaerobe	91

Spore formation

@ref	Spore formation	Confidence
125439		97.1

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	sulfopterin metabolism	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	phenylmercury acetate degradation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	biotin biosynthesis	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	coenzyme A metabolism	100	4 of 4
66794	denitrification	100	2 of 2
66794	flavin biosynthesis	93.33	14 of 15
66794	chorismate metabolism	88.89	8 of 9
66794	CO2 fixation in Crenarchaeota	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	phenylalanine metabolism	84.62	11 of 13
66794	methylglyoxal degradation	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	vitamin B12 metabolism	79.41	27 of 34
66794	photosynthesis	78.57	11 of 14
66794	NAD metabolism	77.78	14 of 18
66794	palmitate biosynthesis	77.27	17 of 22
66794	vitamin B1 metabolism	76.92	10 of 13
66794	isoleucine metabolism	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	citric acid cycle	71.43	10 of 14
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	threonine metabolism	70	7 of 10
66794	d-mannose degradation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cyanate degradation	66.67	2 of 3
66794	purine metabolism	64.89	61 of 94
66794	pyrimidine metabolism	62.22	28 of 45
66794	cellulose degradation	60	3 of 5
66794	creatinine degradation	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	Entner Doudoroff pathway	60	6 of 10
66794	propionate fermentation	60	6 of 10
66794	lipid metabolism	58.06	18 of 31
66794	non-pathway related	57.89	22 of 38
66794	heme metabolism	57.14	8 of 14
66794	serine metabolism	55.56	5 of 9
66794	alanine metabolism	55.17	16 of 29
66794	pentose phosphate pathway	54.55	6 of 11
66794	urea cycle	53.85	7 of 13
66794	leucine metabolism	53.85	7 of 13
66794	methionine metabolism	53.85	14 of 26
66794	sulfate reduction	53.85	7 of 13
66794	glycolysis	52.94	9 of 17
66794	tryptophan metabolism	52.63	20 of 38
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	tyrosine metabolism	50	7 of 14
66794	arginine metabolism	50	12 of 24
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	CDP-diacylglycerol biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	degradation of sugar alcohols	50	8 of 16
66794	cyclohexanol degradation	50	2 of 4
66794	resorcinol degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	glycogen biosynthesis	50	2 of 4
66794	histidine metabolism	48.28	14 of 29
66794	proline metabolism	45.45	5 of 11
66794	lipid A biosynthesis	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	molybdenum cofactor biosynthesis	44.44	4 of 9
66794	cysteine metabolism	44.44	8 of 18
66794	glutathione metabolism	42.86	6 of 14
66794	propanol degradation	42.86	3 of 7
66794	cardiolipin biosynthesis	42.86	3 of 7
66794	aclacinomycin biosynthesis	42.86	3 of 7
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	coenzyme M biosynthesis	40	4 of 10
66794	myo-inositol biosynthesis	40	4 of 10
66794	phenol degradation	40	8 of 20
66794	degradation of hexoses	38.89	7 of 18
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	ketogluconate metabolism	37.5	3 of 8
66794	degradation of pentoses	35.71	10 of 28
66794	polyamine pathway	34.78	8 of 23
66794	isoprenoid biosynthesis	34.62	9 of 26
66794	octane oxidation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	lysine metabolism	33.33	14 of 42
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	1,4-dihydroxy-6-naphthoate biosynthesis	33.33	2 of 6
66794	enterobactin biosynthesis	33.33	1 of 3
66794	oxidative phosphorylation	30.77	28 of 91
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	mevalonate metabolism	28.57	2 of 7
66794	reductive acetyl coenzyme A pathway	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	vitamin B6 metabolism	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	peptidoglycan biosynthesis	26.67	4 of 15
66794	butanoate fermentation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	methanogenesis from CO2	25	3 of 12
66794	alginate biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	degradation of sugar acids	24	6 of 25
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	chlorophyll metabolism	22.22	4 of 18
66794	arachidonic acid metabolism	22.22	4 of 18

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21344	garden soil	Vienna, 9th, district	Austria	AUT	Europe
67770	Garden soil in Vienna

Taxonmaps

69479

Global distribution of 16S sequence FR773157 (>99% sequence identity) for Nitrososphaera viennensis subclade from Microbeatlas

Aquatic Samples	1057
Soil Samples	8092
Animal Samples	237
Plant Samples	1059
Total Samples	10445

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21344	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM69878v1 assembly for Nitrososphaera viennensis EN76	complete	GCA_000698785	926571.24	2585427612	926571	87.21
66792	Nitrososphaera viennensis EN76	complete		926571.7		926571	86.2

Genome browser: GCA_000698785

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21344	Nitrososphaera viennensis EN76 partial 16S rRNA gene, type strain EN76T	FR773157	1419		926571

GC content

@ref	GC-content (mol%)
21344	52.7
67770	52.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Nitrososphaera viennensis EN76
NCBI: GCA_000698785

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.10	no
125439	motility	BacteriaNetⓘ	no	72.40	no
125439	gram_stain	BacteriaNetⓘ	negative	97.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	91.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nitrososphaera viennensis EN76 EN76
NCBI: GCA_000698785.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	67.23	no
125438	anaerobic	anaerobicⓘ	no	59.47	no
125438	aerobic	aerobicⓘ	yes	50.04	no
125438	spore-forming	spore-formingⓘ	no	84.10	no
125438	thermophilic	thermophileⓘ	no	54.02	no
125438	flagellated	motile2+ⓘ	no	85.31	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nitrososphaera viennensis gen. nov., sp. nov., an aerobic and mesophilic, ammonia-oxidizing archaeon from soil and a member of the archaeal phylum Thaumarchaeota.	Stieglmeier M, Klingl A, Alves RJE, Rittmann SKR, Melcher M, Leisch N, Schleper C	Int J Syst Evol Microbiol	10.1099/ijs.0.063172-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21344	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26422
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Nitrososphaera viennensis (DSM 26422, EN76, JCM 19564)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000698785

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media