Hydrogenispora ethanolica (DSM 25471, CGMCC 1.5175, JCM 18117)

Name: Hydrogenispora ethanolica (DSM 25471, CGMCC 1.5175, JCM 18117)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24818

Type strain

Strain Designation LX-B

Culture col. no. DSM 25471 CGMCC 1.5175 JCM 18117 LX-B

NCBI tax ID(s) 1082276

Special Collections DSMZ JCM

History <- L. Yi, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao; LX-B <- L. Yi {2011} Y Liu et al. strain LX-B.

Links

Hydrogenispora ethanolica LX-B is an anaerobe bacterium that was isolated from anaerobic, mesophilic sludge treating herbicide wastewater.

anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400228

JCM 18117,DSM 25471

@ref 20215

Last LPSN update 2026-02-09 11:24:05

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Hydrogenispora

Species Hydrogenispora ethanolica

Full scientific name Hydrogenispora ethanolica Liu et al. 2014

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21333	DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)		Medium recipe at MediaDive	Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641; with strain-specific modifications) Composition: D-Glucose 1.99402 g/l Na2SO4 1.99402 g/l Na2S2O3 x 5 H2O 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
21333	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
21333	anaerobe
125439	anaerobe	96.8

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	glycine betaine biosynthesis	100	5 of 5
66794	chorismate metabolism	100	9 of 9
66794	methylglyoxal degradation	100	5 of 5
66794	kanosamine biosynthesis II	100	2 of 2
66794	cyanate degradation	100	3 of 3
66794	cardiolipin biosynthesis	100	7 of 7
66794	starch degradation	100	10 of 10
66794	ppGpp biosynthesis	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	hydrogen production	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	palmitate biosynthesis	95.45	21 of 22
66794	vitamin B1 metabolism	92.31	12 of 13
66794	valine metabolism	88.89	8 of 9
66794	d-mannose degradation	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	isoleucine metabolism	87.5	7 of 8
66794	photosynthesis	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	purine metabolism	82.98	78 of 94
66794	vitamin B12 metabolism	82.35	28 of 34
66794	pentose phosphate pathway	81.82	9 of 11
66794	peptidoglycan biosynthesis	80	12 of 15
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	degradation of sugar acids	80	20 of 25
66794	flavin biosynthesis	80	12 of 15
66794	threonine metabolism	80	8 of 10
66794	Entner Doudoroff pathway	80	8 of 10
66794	heme metabolism	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	pyrimidine metabolism	77.78	35 of 45
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	alanine metabolism	75.86	22 of 29
66794	ketogluconate metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	degradation of pentoses	75	21 of 28
66794	acetate fermentation	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	degradation of sugar alcohols	75	12 of 16
66794	biotin biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	methionine metabolism	73.08	19 of 26
66794	vitamin B6 metabolism	72.73	8 of 11
66794	degradation of hexoses	72.22	13 of 18
66794	propanol degradation	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	oxidative phosphorylation	68.13	62 of 91
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	enterobactin biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	methane metabolism	66.67	2 of 3
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	tetrahydrofolate metabolism	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	non-pathway related	63.16	24 of 38
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	leucine metabolism	61.54	8 of 13
66794	sulfate reduction	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	myo-inositol biosynthesis	60	6 of 10
66794	propionate fermentation	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	arginine metabolism	58.33	14 of 24
66794	citric acid cycle	57.14	8 of 14
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	polyamine pathway	56.52	13 of 23
66794	tryptophan metabolism	55.26	21 of 38
66794	urea cycle	53.85	7 of 13
66794	histidine metabolism	51.72	15 of 29
66794	lactate fermentation	50	2 of 4
66794	toluene degradation	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	glutathione metabolism	50	7 of 14
66794	butanoate fermentation	50	2 of 4
66794	coenzyme M biosynthesis	50	5 of 10
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	sphingosine metabolism	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	lysine metabolism	47.62	20 of 42
66794	proline metabolism	45.45	5 of 11
66794	metabolism of disaccharids	45.45	5 of 11
66794	ascorbate metabolism	45.45	10 of 22
66794	nitrate assimilation	44.44	4 of 9
66794	tyrosine metabolism	42.86	6 of 14
66794	lipid metabolism	41.94	13 of 31
66794	phenol degradation	40	8 of 20
66794	creatinine degradation	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	vitamin K metabolism	40	2 of 5
66794	IAA biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	4-hydroxyphenylacetate degradation	30	3 of 10
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	alginate biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	catecholamine biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Waste	#Wastewater
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21333	anaerobic, mesophilic sludge treating herbicide wastewater	Qingdao	China	CHN	Asia
67770	Anaerobic sludge treating herbicide wastewater

Taxonmaps

69479

Global distribution of 16S sequence AB669474 (>99% sequence identity) for Hydrogenispora ethanolica subclade from Microbeatlas

Aquatic Samples	236
Soil Samples	574
Animal Samples	191
Plant Samples	50
Total Samples	1051

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21333	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM434068v1 assembly for Hydrogenispora ethanolica LX-B	scaffold	GCA_004340685	1082276.3	2788499852	1082276	54.33

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21333	Hydrogenispora ethanolica gene for 16S rRNA, partial sequence	AB669474	1443		1082276

GC content

@ref	GC-content (mol%)	Method
21333	56.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Hydrogenispora ethanolica LX-B
NCBI: GCA_004340685

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	76.60	no
125439	motility	BacteriaNetⓘ	yes	57.30	no
125439	gram_stain	BacteriaNetⓘ	positive	61.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	96.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Hydrogenispora ethanolica LX-B
NCBI: GCA_004340685.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	79.39	no
125438	anaerobic	anaerobicⓘ	yes	82.22	no
125438	aerobic	aerobicⓘ	no	89.32	yes
125438	spore-forming	spore-formingⓘ	yes	60.22	no
125438	thermophilic	thermophileⓘ	no	82.32	yes
125438	flagellated	motile2+ⓘ	yes	75.96	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Bioaugmentation of Hydrogenispora ethanolica LX-B affects hydrogen production through altering indigenous bacterial community structure.	Yang Z, Guo R, Shi X, He S, Wang L, Dai M, Qiu Y, Dang X	Bioresour Technol	10.1016/j.biortech.2016.03.097	2016
Phylogeny	Capillibacterium thermochitinicola gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost.	Ungkulpasvich U, Baramee S, Uke A, Kosugi A	Int J Syst Evol Microbiol	10.1099/ijsem.0.004693	2021
Phylogeny	Hydrogenispora ethanolica gen. nov., sp. nov., an anaerobic carbohydrate-fermenting bacterium from anaerobic sludge.	Liu Y, Qiao JT, Yuan XZ, Guo RB, Qiu YL	Int J Syst Evol Microbiol	10.1099/ijs.0.060186-0	2014

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21333	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25471
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Hydrogenispora ethanolica (DSM 25471, CGMCC 1.5175, JCM 18117)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004340685

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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