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Strain identifier

BacDive ID: 24818

Type strain: Yes

Species: Hydrogenispora ethanolica

Strain Designation: LX-B

Strain history: Y Liu et al. strain LX-B.

NCBI tax ID(s): 1082276 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 21333

BacDive-ID: 24818

DSM-Number: 25471

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Hydrogenispora ethanolica LX-B is an anaerobe, mesophilic bacterium that was isolated from anaerobic, mesophilic sludge treating herbicide wastewater.

NCBI tax id

  • NCBI tax id: 1082276
  • Matching level: species

strain history

doi: 10.13145/bacdive24818.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Hydrogenispora
  • species: Hydrogenispora ethanolica
  • full scientific name: Hydrogenispora ethanolica Liu et al. 2014

@ref: 21333

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Firmicutes, not assigned to order

family: Clostridiaceae

genus: Hydrogenispora

species: Hydrogenispora ethanolica

full scientific name: Hydrogenispora ethanolica Liu et al. 2014

strain designation: LX-B

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 96.246

Culture and growth conditions

culture medium

  • @ref: 21333
  • name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/641
  • composition: Name: DESULFOVIBRIO (MV) MEDIUM (DSMZ Medium 641; with strain-specific modifications) Composition: D-Glucose 1.99402 g/l Na2SO4 1.99402 g/l Na2S2O3 x 5 H2O 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l NH4Cl 0.997009 g/l Yeast extract 0.997009 g/l Na2CO3 0.997009 g/l KH2PO4 0.498504 g/l Na2S x 9 H2O 0.0997009 g/l CaCl2 x 2 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21333positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
21333anaerobe
69480anaerobe99.696

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 73.622

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 98.311

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21333anaerobic, mesophilic sludge treating herbicide wastewaterQingdaoChinaCHNAsia
67770Anaerobic sludge treating herbicide wastewater

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Mud (Sludge)
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_177608.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_882;96_65790;97_85414;98_115988;99_177608&stattab=map
  • Last taxonomy: Hydrogenispora ethanolica subclade
  • 16S sequence: AB669474
  • Sequence Identity:
  • Total samples: 1051
  • soil counts: 574
  • aquatic counts: 236
  • animal counts: 191
  • plant counts: 50

Safety information

risk assessment

  • @ref: 21333
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21333
  • description: Hydrogenispora ethanolica gene for 16S rRNA, partial sequence
  • accession: AB669474
  • length: 1443
  • database: ena
  • NCBI tax ID: 1082276

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Hydrogenispora ethanolica LX-BGCA_004340685scaffoldncbi1082276
66792Hydrogenispora ethanolica strain LX-B1082276.3wgspatric1082276
66792Hydrogenispora ethanolica DSM 254712788499852draftimg1082276
66792Verrucomicrobia bacterium CP_BM_ER_71GCA_019246215contigpatric2026799

GC content

  • @ref: 21333
  • GC-content: 56.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 21333

culture collection no.: DSM 25471, CGMCC 1.5175, JCM 18117

straininfo link

@refpassport
20218http://www.straininfo.net/strains/896349
20218http://www.straininfo.net/strains/896351
20218http://www.straininfo.net/strains/896350

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24554637Hydrogenispora ethanolica gen. nov., sp. nov., an anaerobic carbohydrate-fermenting bacterium from anaerobic sludge.Liu Y, Qiao JT, Yuan XZ, Guo RB, Qiu YLInt J Syst Evol Microbiol10.1099/ijs.0.060186-02014Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Molecular Sequence Data, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Water/*microbiologyGenetics
Metabolism27023388Bioaugmentation of Hydrogenispora ethanolica LX-B affects hydrogen production through altering indigenous bacterial community structure.Yang Z, Guo R, Shi X, He S, Wang L, Dai M, Qiu Y, Dang XBioresour Technol10.1016/j.biortech.2016.03.0972016Bioreactors/*microbiology, Firmicutes/*metabolism, *Hydrogen/analysis/metabolism, Sewage/*microbiologyBiotechnology
Phylogeny33724176Capillibacterium thermochitinicola gen. nov., sp. nov., a novel anaerobic thermophilic chitinolytic bacterium from compost.Ungkulpasvich U, Baramee S, Uke A, Kosugi AInt J Syst Evol Microbiol10.1099/ijsem.0.0046932021

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.StrainInfo introduces electronic passports for microorganisms.10.1016/j.syapm.2013.11.002Syst Appl Microbiol. 37: 42-50 201424321274
21333Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-25471Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25471)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes