Name: Burkholderia cepacia 717
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1906

Type strain:

Species: Burkholderia cepacia

Strain Designation: 717

Culture col. no.: DSM 7288, ATCC 25416, DSM 9897, ICPB PC25, NCTC 10743, JCM 5964, BCRC 13208, CCUG 12691, CCUG 13226, CECT 4137, CGMCC 1.1813, CGMCC 1.3393, CIP 80.24, IFO 14074, KCTC 2966, LMG 1222, NBRC 14074, NRRL B-14810, VTT E-94512

Strain history: Y. Kosako 85005 <-- Nissui Pharm. Co., Ltd. <-- ATCC 25416 <-- R. W. Ballard 717 <-- M. Starr ICPB PC 25 <-- W. Burkholder.

NCBI tax ID(s): 983594 (strain), 292 (species)

Other strains from Burkholderia cepacia

Section

Burkholderia cepacia 717 is a mesophilic human pathogen that has multiple antibiotic resistances and was isolated from Onion.

antibiotic resistance
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia cepacia**
	Full Scientific Name (PNU)	Burkholderia cepacia (Palleroni and Holmes 1981) Yabuuchi et al. 1993

Synonym

Pseudomonas cepacia

Information on morphological and physiological properties Morphology

[Ref.: #3094]	Type of hemolysis	gamma
[Ref.: #3094]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3094]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3094]

Culture medium growth

[Ref.: #3094]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #3094]

Culture medium composition

expand / minimize

[Ref.: #3094]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #3094]

Culture medium growth

[Ref.: #3094]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #3094]

Culture medium composition

expand / minimize

[Ref.: #37582]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37582]

Culture medium growth

[Ref.: #37582]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #3094]	growth	28 °C
[Ref.: #37582]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #3094]	Temperature range	mesophilic
[Ref.: #37582]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Reference

[Ref.: #3094]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
resistant0
22-24
resistant0
resistant0
22
20
18
10
20-22
resistant0
10
resistant0
resistant0
resistant0
20
20
resistant0
12
resistant0
resistant0
resistant0
12
10
20
22
resistant0
resistant0
26
resistant0
resistant0
10
resistant0
28

[Ref.: #3094]
Mueller-Hinton Agar
30

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
resistant0
22-24
resistant0
resistant0
22
20
18
10
20-22
resistant0
10
resistant0
resistant0
resistant0
20
20
resistant0
12
resistant0
resistant0
resistant0
12
10
20
22
resistant0
resistant0
26
resistant0
resistant0
10
resistant0
28

[Ref.: #3094]
Mueller Hinton
30

aerob
yes
Inhibition zone diameter in mm
resistant0
resistant0
resistant0
resistant0
22
resistant0
resistant0
20
20
14
6
20-22
resistant0
10
resistant0
6
resistant0
18-20
16-18
resistant0
10-12
resistant0
resistant0
resistant0
14-16
10
20
22
resistant0
resistant0
22-24
resistant0
resistant0
8-10
resistant0
28-30

Only first 15 entries are displayed. Click here to see all.

[Ref.: #3094]

Name of produced compound

cepabactin

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #3094]	catalase	+	1.11.1.6
[Ref.: #3094]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #3094]	22631	-	-	+	-	-	+/-	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #3094]	22356	-	-	-	-	-	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #3094]	22112	-	-	-	-	-	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	-
[Ref.: #3094]	21893	-	-	-	-	-	+	-	+	+	+	+	+	+	+/-	+	+	+	+	+	+	+
[Ref.: #3094]	20990	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #3094]	20989	-	-	-	-	-	-	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #3094]	20988	-	-	-	-	-	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	not determinedn.d.
[Ref.: #3094]	20987	-	-	-	-	-	+	-	+	+	+	+	+	+	-	+	+	+	+	+/-	+	not determinedn.d.
[Ref.: #3094]	20986	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	not determinedn.d.
[Ref.: #3094]	20985	-	-	-	-	-	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+
[Ref.: #3094]	20984	-	-	-	-	-	+/-	-	+	+	+	+	+	+	+/-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3094]	Sample type/isolated from	Onion

[Ref.: #67770]	Sample type/isolated from	Onion
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence U96927 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3094]	Pathogenicity (human)	yes
[Ref.: #3094]	Pathogenicity (animal)	yes
[Ref.: #3094]	Pathogenicity (plant)	yes
[Ref.: #3094]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Burkholderia cepacia strain ATCC 25416 16S ribosomal RNA gene, partial sequence	AY728197	687	ENA	983594 tax ID	*
[Ref.: #20218]	Burkholderia cepacia gene for 16S rRNA, partial sequence, strain: ATCC 25416 (= EY 645)	D13046	158	ENA	983594 tax ID	*
[Ref.: #20218]	Burkholderia cepacia ATCC 25416 strain LMG 1222 16S ribosomal RNA gene, partial sequence	HQ849078	1124	ENA	983594 tax ID	*
[Ref.: #20218]	P.cepacia 16S ribosomal RNA, complete cds	M22518	1473	ENA	292 tax ID	*
[Ref.: #20218]	Burkholderia cepacia 16S ribosomal RNA gene, partial sequence	U96927	1610	ENA	983594 tax ID	*
[Ref.: #3094]	Burkholderia cepacia strain ATCC25416 16S ribosomal RNA gene, partial sequence	AF097530	1462	ENA	983594 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia cepacia ATCC 25416 UCB 717	GCA_001411495	complete	GenBank	983594 tax ID	*
[Ref.: #66792]	Burkholderia cepacia ATCC 25416 ATCC 25416	GCA_006094315	complete	GenBank	983594 tax ID	*
[Ref.: #66792]	Burkholderia cepacia ATCC 25416 ATCC 25416	GCA_003546465	complete	GenBank	983594 tax ID	*
[Ref.: #66792]	Burkholderia cepacia NBRC 14074	GCA_001528845	contig	GenBank	292 tax ID	*
[Ref.: #66792]	Burkholderia cepacia ATCC 25416 ATCC 25416	GCA_000473485	scaffold	GenBank	983594 tax ID	*
[Ref.: #66792]	Burkholderia cepacia ATCC 25416	983594.7	wgs	PATRIC	983594 tax ID	*

[Ref.: #3094]

GC-content

67.8 mol%

Buoyant density centrifugation (BD)

Availability in culture collections External links

[Ref.: #3094]

Culture collection no.

DSM 7288, ATCC 25416, DSM 9897, ICPB PC25, NCTC 10743, JCM 5964, BCRC 13208, CCUG 12691, CCUG 13226, CECT 4137, CGMCC 1.1813, CGMCC 1.3393, CIP 80.24, IFO 14074, KCTC 2966, LMG 1222, NBRC 14074, NRRL B-14810, VTT E-94512

[Ref.: #20218]

Associated Passport(s) in StrainInfo

43439, 43431, 43442, 43446

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Cepabactin from Pseudomonas cepacia, a new type of siderophore.	Meyer JM, Hohnadel D, Halle F	J Gen Microbiol	10.1099/00221287-135-6-1479	1989	*
Metabolism	Occurrence of succinyl derivatives in the catabolism of arginine in Pseudomonas cepacia.	Vander Wauven C, Stalon V	J Bacteriol	10.1128/jb.164.2.882-886.1985	1985	*
Phenotype	[Phenotype characteristics of 100 strains of Pseudomonas cepacia. Proposition of a biovars classification (author's transl)].	Richard C, Monteil H, Megraud F, Chatelain R, Laurent B	Ann Biol Clin (Paris)		1981	*
Enzymology	A physical genome map of the Burkholderia cepacia type strain.	Rodley PD, Romling U, Tummler B	Mol Microbiol	10.1111/j.1365-2958.1995.mmi_17010057.x	1995	*
Metabolism	Isolation of uracil auxotrophs from Burkholderia cepacia ATCC 25416.	Li K, West TP	Lett Appl Microbiol	10.1111/j.1472-765x.1995.tb01074.x	1995	*
Metabolism	Pyrimidine synthesis in Burkholderia cepacia ATCC 25416.	Li K, West TP	Lett Appl Microbiol	10.1111/j.1472-765x.1995.tb01075.x	1995	*
Metabolism	Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia).	Meyer JM, Van VT, Stintzi A, Berge O, Winkelmann G	Biometals	10.1007/BF00141604	1995	*
Phylogeny	Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov.	Grimes DJ, Woese CR, MacDonell MT, Colwell RR	Int J Syst Bacteriol	10.1099/00207713-47-1-19	1997	*
Enzymology	Cloning and expression of the major porin protein gene opcP of Burkholderia (formerly Pseudomonas) cepacia in Escherichia coli.	Tsujimoto H, Gotoh N, Yamagishi J, Oyamada Y, Nishino T	Gene	10.1016/s0378-1119(96)00692-0	1997	*
Enzymology	Mobilization, cloning, and sequence determination of a plasmid-encoded polygalacturonase from a phytopathogenic Burkholderia (Pseudomonas) cepacia.	Gonzalez CF, Pettit EA, Valadez VA, Provin EM	Mol Plant Microbe Interact	10.1094/MPMI.1997.10.7.840	1997	*
Phylogeny	Isolation and characterisation of disodium (4-amino-4-deoxy-beta-L- arabinopyranosyl)-(1-->8)-(D-glycero-alpha-D-talo-oct-2-ulopyranosylona te)- (2-->4)-(methyl 3-deoxy-D-manno-oct-2-ulopyranosid)onate from the lipopolysaccharide of Burkholderia cepacia.	Isshiki Y, Kawahara K, Zahringer U	Carbohydr Res	10.1016/s0008-6215(98)00179-7	1998	*
Phylogeny	Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients.	LiPuma JJ, Dulaney BJ, McMenamin JD, Whitby PW, Stull TL, Coenye T, Vandamme P	J Clin Microbiol	10.1128/JCM.37.10.3167-3170.1999	1999	*
Metabolism	Role of cytosine deaminase and beta-alanine-pyruvate transaminase in pyrimidine base catabolism by Burkholderia cepacia.	West TP	Antonie Van Leeuwenhoek	10.1023/a:1002062202282	2000	*
Enzymology	Construction of a deep-rough mutant of Burkholderia cepacia ATCC 25416 and characterization of its chemical and biological properties.	Gronow S, Noah C, Blumenthal A, Lindner B, Brade H	J Biol Chem	10.1074/jbc.M206942200	2002	*
Metabolism	Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416.	Aguilar C, Bertani I, Venturi V	Appl Environ Microbiol	10.1128/AEM.69.3.1739-1747.2003	2003	*
Metabolism	Transmissible Burkholderia cepacia genomovar IIIa strains bind and convert monomeric iron(III) protoporphyrin IX into the mu-oxo oligomeric form.	Smalley JW, Charalabous P, Hart CA, Silver J	Microbiology (Reading)	10.1099/mic.0.26160-0	2003	*
Metabolism	Identification of quorum-sensing-regulated genes of Burkholderia cepacia.	Aguilar C, Friscina A, Devescovi G, Kojic M, Venturi V	J Bacteriol	10.1128/JB.185.21.6456-6462.2003	2003	*
Pathogenicity	Identification of an RTX determinant of Burkholderia cenocepacia J2315 by subtractive hybridization.	Whitby PW, VanWagoner TM, Taylor AA, Seale TW, Morton DJ, LiPuma JJ, Stull TL	J Med Microbiol	10.1099/jmm.0.46138-0	2006	*
Enzymology	Isolation, heterologous expression and characterization of an endo-polygalacturonase produced by the phytopathogen Burkholderia cepacia.	Massa C, Degrassi G, Devescovi G, Venturi V, Lamba D	Protein Expr Purif	10.1016/j.pep.2007.03.019	2007	*
Cultivation	Influence of Legionella pneumophila and other water bacteria on the survival and growth of Acanthamoeba polyphaga.	Anacarso I, Guerrieri E, Bondi M, de Niederhausern S, Iseppi R, Sabia C, Contri M, Borella P, Messi P	Arch Microbiol	10.1007/s00203-010-0618-0	2010	*
Metabolism	Application of lipase from Burkholderia cepacia in the degradation of agro-industrial effluent.	Mello Bueno PR, de Oliveira TF, Castiglioni GL, Soares Junior MS, Ulhoa CJ	Water Sci Technol	10.2166/wst.2015.037	2015	*
Enzymology	Enantioselective synthesis of a chiral intermediate of himbacine analogs by Burkholderia cepacia lipase A.	Chen Y, Gao F, Zheng G, Gao S	Biotechnol Lett	10.1007/s10529-020-02969-z	2020	*
Pathogenicity	Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses.	Kim SY, Kim MH, Son JH, Kim SI, Yun SH, Kim K, Kim S, Shin M, Lee JC	Virulence	10.1080/21505594.2020.1802193	2020	*
	Antibacterial effects of microbial synthesized silver-copper nanoalloys on Escherichia coli, Burkholderia cepacia, Listeria monocytogenes and Brucella abortus.	Mohammadi S, Jazani NH, Kouhkan M, Babaganjeh LA	Iran J Microbiol		2018	*
	First Report of Bacterial Leaf Streak of Strelitzia reginae Caused by Burkholderia cepacia.	You CP, Xiang MM, Zhang YX	Plant Dis	10.1094/PDIS-02-13-0205-PDN	2014	*

References

#3094

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7288

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37582 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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