Name: Sulfurisphaera javensis KD-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158817

Type strain:

Species: Sulfurisphaera javensis

Strain Designation: KD-1

Culture col. no.: JCM 32117, InaCC Ar81

Strain history: N. Kurosawa; Fac. of Sci. & Eng., Soka Univ., Japan; KD-1 <-- A. Suwanto.

NCBI tax ID(s): 2049879 (species)

Special Collections

Section

Sulfurisphaera javensis KD-1 is a facultative anaerobe, chemolithoautotroph, coccus-shaped archaeon that forms circular colonies and was isolated from hot spring water.

coccus-shaped
colony-forming
chemolithoautotroph
facultative anaerobe
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Sulfolobales*
	Family	*Sulfolobaceae*
	Genus	*Sulfurisphaera*
	Species	**Sulfurisphaera javensis**
	Full Scientific Name (LPSN)	Sulfurisphaera javensis Tsuboi et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65581]	Cell length	0.9-1.3 µm

[Ref.: #65581]	Cell shape	coccus-shaped

[Ref.: #65581]	Colony size	1.0-1.3 mm
[Ref.: #65581]	Colony color	light yellow
[Ref.: #65581]	Colony shape	circular
[Ref.: #65581]	Incubation period	13 days
[Ref.: #65581]	Cultivation medium used	modified Brock's basal salt

Information on culture and growth conditions Culture and growth conditions

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Culture medium

modified Brock's basal salt

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Culture medium growth

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Culture medium

modified Brock's basal salt

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #65581]	growth	60-90 °C
[Ref.: #65581]	optimum	80-85 °C
[Ref.: #67770]	growth	80 °C

	pH	Kind of pH		pH
[Ref.: #65581]			optimum	3.5-4.0
[Ref.: #65581]			growth	2.5-6.0

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

facultative anaerobe

[Ref.: #65581]	Nutrition type	chemolithoautotroph
[Ref.: #65581]	Nutrition type	organotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65581]		NaCl		growth	0-1.0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65581]	alanine ChEBI	-	growth
[Ref.: #65581]	aspartate ChEBI	-	growth
[Ref.: #65581]	beef extract	+	growth
[Ref.: #65581]	casamino acids	+	growth
[Ref.: #65581]	cysteine ChEBI	-	growth
[Ref.: #65581]	D-fructose ChEBI	-	growth
[Ref.: #65581]	D-galactose ChEBI	-	growth
[Ref.: #65581]	D-glucose ChEBI	-	growth
[Ref.: #65581]	D-xylose ChEBI	-	growth
[Ref.: #65581]	dihydrogen ChEBI	+	growth
[Ref.: #65581]	elemental sulfur ChEBI	+	growth
[Ref.: #65581]	glutamate ChEBI	-	growth
[Ref.: #65581]	glutamine ChEBI	-	growth
[Ref.: #65581]	glycine ChEBI	-	growth
[Ref.: #65581]	histidine ChEBI	-	growth
[Ref.: #65581]	iron trichloride ChEBI	+	growth
[Ref.: #65581]	L-arabinose ChEBI	-	growth
[Ref.: #65581]	lactose ChEBI	-	growth
[Ref.: #65581]	maltose ChEBI	-	growth
[Ref.: #65581]	peptone	+	growth
[Ref.: #65581]	pyrite ChEBI	+	growth
[Ref.: #65581]	raffinose ChEBI	-	growth
[Ref.: #65581]	sodium thiosulfate ChEBI	+	growth
[Ref.: #65581]	sorbose ChEBI	-	growth
[Ref.: #65581]	sucrose ChEBI	-	growth
[Ref.: #65581]	tetrathionate ChEBI	+	growth
[Ref.: #65581]	tryptone	+	growth
[Ref.: #65581]	tryptophan ChEBI	-	growth
[Ref.: #65581]	tyrosine ChEBI	-	growth
[Ref.: #65581]	yeast extract (0.01 %, w/v)	+	growth
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65581]	Sample type/isolated from	hot spring water
[Ref.: #65581]	Sampling date	2015-04-21
[Ref.: #65581]	Geographic location (country and/or sea, region)	Kawah Domas
[Ref.: #65581]	Country	Indonesia
[Ref.: #65581]	Country ISO 3 Code	IDN
[Ref.: #65581]	Continent	Asia
[Ref.: #65581]	Geographic location	-6.7614°/107.6260°
[Ref.: #65581]	Enrichment culture	modified Brock's basal salt medium
[Ref.: #65581]	Enrichment culture composition	supplemented with yeast extract (1g/l), pH 4.0
[Ref.: #65581]	Enrichment culture temperature	75 ̊C

[Ref.: #67770]	Sample type/isolated from	Acidic hot spring in Indonesia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65581]	Sulfurisphaera javensis gene for 16S ribosomal RNA, partial sequence	LC332080		GenBank ENA

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GC-content

30.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65581]

Culture collection no.

JCM 32117, InaCC Ar81

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.	Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.002765	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65581

Kazuhiko Tsuboi, Hiroyuki D. Sakai, Naswandi Nur, Kenneth M. Stedman, Norio Kurosawa and Antonius Suwanto: Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.. IJSEM 68: 1907 - 1913 2018 ( DOI 10.1099/ijsem.0.002765 , PubMed 29671720 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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