Strain identifier

BacDive ID: 158817

Type strain: Yes

Species: Sulfurisphaera javensis

Strain Designation: KD-1

Strain history: N. Kurosawa; Fac. of Sci. & Eng., Soka Univ., Japan; KD-1 <-- A. Suwanto.

NCBI tax ID(s): 2049879 (species)

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General

@ref: 65581

BacDive-ID: 158817

keywords: 16S sequence, Archaea, facultative anaerobe, chemolithoautotroph, hyperthermophilic, coccus-shaped, colony-forming

description: Sulfurisphaera javensis KD-1 is a facultative anaerobe, chemolithoautotroph, hyperthermophilic archaeon that forms circular colonies and was isolated from hot spring water.

NCBI tax id

  • NCBI tax id: 2049879
  • Matching level: species

strain history

  • @ref: 67770
  • history: N. Kurosawa; Fac. of Sci. & Eng., Soka Univ., Japan; KD-1 <-- A. Suwanto.

doi: 10.13145/bacdive158817.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/thermoproteota
  • domain: Archaea
  • phylum: Thermoproteota
  • class: Thermoprotei
  • order: Sulfolobales
  • family: Sulfolobaceae
  • genus: Sulfurisphaera
  • species: Sulfurisphaera javensis
  • full scientific name: Sulfurisphaera javensis Tsuboi et al. 2018

@ref: 65581

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Sulfolobales

family: Sulfolobaceae

genus: Sulfurisphaera

species: Sulfurisphaera javensis

strain designation: KD-1

type strain: yes

Morphology

cell morphology

  • @ref: 65581
  • cell length: 0.9-1.3 µm
  • cell shape: coccus-shaped

colony morphology

  • @ref: 65581
  • colony size: 1.0-1.3 mm
  • colony color: light yellow
  • colony shape: circular
  • incubation period: 13 days
  • medium used: modified Brock's basal salt

Culture and growth conditions

culture medium

@refnamegrowthcomposition
65581modified Brock's basal saltyes
65581modified Brock's basal saltyessupplemented with yeast extract (1g/l), MgSO4 (10 mmol/l), CaCl2 (2.5 mmol/l), gellan gum (6.6 g/l), pH 4.0

culture temp

@refgrowthtypetemperaturerange
65581positivegrowth60-90hyperthermophilic
65581positiveoptimum80-85hyperthermophilic
67770positivegrowth80hyperthermophilic

culture pH

@refabilitytypepHPH range
65581positivegrowth2.5-6.0acidophile
65581positiveoptimum3.5-4.0

Physiology and metabolism

oxygen tolerance

  • @ref: 65581
  • oxygen tolerance: facultative anaerobe

nutrition type

@reftype
65581chemolithoautotroph
65581organotroph

halophily

  • @ref: 65581
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6558116449alanine-growth
6558135391aspartate-growth
65581beef extract+growth
65581casamino acids+growth
6558115356cysteine-growth
6558115824D-fructose-growth
6558112936D-galactose-growth
6558117634D-glucose-growth
6558165327D-xylose-growth
6558118276dihydrogen+growth
6558133403elemental sulfur+growth
6558129987glutamate-growth
6558128300glutamine-growth
6558115428glycine-growth
6558127570histidine-growth
6558130808iron trichloride+growth
6558130849L-arabinose-growth
6558117716lactose-growth
6558117306maltose-growth
65581peptone+growth
6558186471pyrite+growth
6558116634raffinose-growth
65581132112sodium thiosulfate+growth
6558127922sorbose-growth
6558117992sucrose-growth
6558115226tetrathionate+growth
65581tryptone+growth
6558127897tryptophan-growth
6558118186tyrosine-growth
65581yeast extract (0.01 %, w/v)+growth

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture temperature
65581hot spring water2015-04-21Kawah DomasIndonesiaIDNAsia-6.76139107.626modified Brock's basal salt mediumsupplemented with yeast extract (1g/l), pH 4.075
67770Acidic hot spring in Indonesia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Sequence information

16S sequences

  • @ref: 65581
  • description: 16S rRNA gene sequence
  • accession: LC332080
  • database: nuccore

GC content

  • @ref: 65581
  • GC-content: 30.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65581

culture collection no.: JCM 32117, InaCC Ar81

literature

  • topic: Phylogeny
  • Pubmed-ID: 29671720
  • title: Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.
  • authors: Tsuboi K, Sakai HD, Nur N, Stedman KM, Kurosawa N, Suwanto A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002765
  • year: 2018
  • mesh: Base Composition, Chemoautotrophic Growth, DNA, Archaeal/genetics, Hot Springs/*microbiology, Indonesia, Lipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfolobaceae/*classification/genetics/isolation & purification, Sulfolobus
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65581Kazuhiko Tsuboi, Hiroyuki D. Sakai, Naswandi Nur, Kenneth M. Stedman, Norio Kurosawa and Antonius SuwantoSulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.10.1099/ijsem.0.002765IJSEM 68: 1907-1913 201829671720
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/