Name: Apilactobacillus micheneri Hlig3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158413

Type strain:

Species: Apilactobacillus micheneri

Strain Designation: Hlig3

Culture col. no.: DSM 104126, NRRL B-65473, JCM 33323

Strain history: DSM 104126 <-- J. A. Rothman, Univ. of California, Riverside, USA; Hlig3.

NCBI tax ID(s): 1899430 (species)

SI-ID 407777

DSM 104126

Special Collections

Section

Apilactobacillus micheneri Hlig3 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from Adult bee gut, Halictus ligatus.

colony-forming
Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Apilactobacillus*
	Species	**Apilactobacillus micheneri**
	Full Scientific Name (LPSN)	Apilactobacillus micheneri (McFrederick et al. 2018) Zheng et al. 2020

	Synonym	Apilactobacillus kosoi
	Synonym	Lactobacillus micheneri
	Synonym	Lactobacillus kosoi

Information on morphological and physiological properties Morphology

[Ref.: #65392]	Gram stain	positive

[Ref.: #65392]	Cell length	0.9-1.6 µm

[Ref.: #65392]	Cell width	0.6-0.8 µm

[Ref.: #65392]	Cell shape	rod-shaped

[Ref.: #65392]	Motility	no

[Ref.: #65392]	Colony color	white
[Ref.: #65392]	Colony shape	circular
[Ref.: #65392]	Cultivation medium used	De Man, Rogosa, Sharpe agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65089]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #65089]

Culture medium growth

[Ref.: #65089]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65089]

Culture medium composition

expand / minimize

[Ref.: #65392]

Culture medium

De Man, Rogosa, Sharpe agar

[Ref.: #65392]

Culture medium growth

[Ref.: #65392]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65089]	growth	30 °C
[Ref.: #65392]	growth	15-35 °C
[Ref.: #65392]	optimum	30-35 °C
[Ref.: #67770]	growth	30 °C

	Kind of pH		pH
[Ref.: #65392]		optimum	6
[Ref.: #65392]		growth	2-6
[Ref.: #65392]	no	growth	7
[Ref.: #65392]	no	growth	>7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65392]

Oxygen tolerance

aerobe

[Ref.: #65392]

Ability of spore formation

[Ref.: #65392]

Observation

heterofermentive

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65392]	D-fructose ChEBI	+	builds acid from
[Ref.: #65392]	D-glucose ChEBI	+	builds acid from
[Ref.: #65392]	glucose ChEBI	+	fermentation

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65392]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #65392]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #65392]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65392]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #65392]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #65392]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65392]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #65392]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #65392]	polymyxin b ChEBI	yes	300	Unit (disc)
[Ref.: #65392]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #65392]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #65392]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65392]	acid phosphatase	+	3.1.3.2
[Ref.: #65392]	catalase	+	1.11.1.6
[Ref.: #65392]	leucine arylamidase	+	3.4.11.1
[Ref.: #65392]	naphthol-AS-BI-phosphohydrolase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65089]	Sample type/isolated from	Adult bee gut, Halictus ligatus
[Ref.: #65089]	Geographic location (country and/or sea, region)	30.2357N, 97.6467W
[Ref.: #65089]	Country	USA
[Ref.: #65089]	Country ISO 3 Code	USA
[Ref.: #65089]	Continent	North America

[Ref.: #65392]	Sample type/isolated from	gut of a sweat bee (Halictus ligatus)
[Ref.: #65392]	Geographic location (country and/or sea, region)	Hornsby Bend Center for Environmental Research, Austin, TX
[Ref.: #65392]	Country	USA
[Ref.: #65392]	Country ISO 3 Code	USA
[Ref.: #65392]	Continent	North America
[Ref.: #65392]	Enrichment culture	De Man, Rogosa, Sharpe agar
[Ref.: #65392]	Enrichment culture composition	supplemented with 20 % D-fructose
[Ref.: #65392]	Enrichment culture duration	2-3 days
[Ref.: #65392]	Enrichment culture temperature	24 ̊C
[Ref.: #65392]	Isolation/enrichment procedure	Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C.

[Ref.: #67770]	Sample type/isolated from	Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental Research
[Ref.: #67770]	Host species	Halictus ligatus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Austin, TX
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65089]	Apilactobacillus micheneri strain Hlig3 16S ribosomal RNA gene, partial sequence	KT833121	1549	GenBank ENA	1899430 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Apilactobacillus micheneri Hlig3	GCA_002993975	contig	GenBank ENA	1899430 tax ID
[Ref.: #66792]	Apilactobacillus micheneri Hlig3	2923762712	draft	JGI IMG/M	1899430 tax ID	*

[Ref.: #65089]	GC-content	30.7 mol%
[Ref.: #65392]	GC-content	30.7 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #65089]

Culture collection no.

DSM 104126, NRRL B-65473, JCM 33323

[Ref.: #111216]

SI-ID 407777

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers.	McFrederick QS, Vuong HQ, Rothman JA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002758	2018	*
	Exploring the genome of Lactobacillaceae spp. Sy-1 isolated from Heterotrigona itama honey.	Syed Yaacob SN, Huyop F, Misson M, Abdul Wahab R, Huda N	PeerJ	10.7717/peerj.13053	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65089

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104126

#65392

Quinn S. McFrederick, Hoang Q. Vuong, Jason A. Rothman: Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. IJSEM 68: 1879 - 1884 2018 ( DOI 10.1099/ijsem.0.002758 , PubMed 29648528 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#111216

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407777.1 )

* These data were automatically processed and therefore are not curated

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