Name: Streptococcus respiraculi HTS25
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158414

Type strain:

Species: Streptococcus respiraculi

Strain Designation: HTS25

Culture col. no.: DSM 101998, CGMCC 1.15531

Strain history: <- L. Niu, Nat. Inst. for Communicable Disease Control and Prevention, Beijing, China; HTS25

NCBI tax ID(s): 2021971 (species)

SI-ID 401235

DSM 101998

Special Collections

Section

Streptococcus respiraculi HTS25 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and builds arranged in pairs, short chains or small groups.

coccus-shaped
colony-forming
Gram-positive
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Streptococcus*
	Species	**Streptococcus respiraculi**
	Full Scientific Name (LPSN)	Streptococcus respiraculi Niu et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66574]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.046

[Ref.: #66574]	Cell length	0.5-1.0 µm

[Ref.: #66574]	Cell shape	coccus-shaped

[Ref.: #66574]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.479

[Ref.: #66574]	Colony size	0.5-1 mm
[Ref.: #66574]	Colony color	non-pigmented
[Ref.: #66574]	Colony shape	circular
[Ref.: #66574]	Incubation period	1 day
[Ref.: #66574]	Cultivation medium used	Columbia blood agar

[Ref.: #66574]	Multicellular complex forming ability	yes
[Ref.: #66574]	Multicellular complex name	arranged in pairs, short chains or small groups

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65090]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #65090]

Culture medium growth

[Ref.: #65090]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65090]

Culture medium composition

expand / minimize

[Ref.: #66574]

Culture medium

Columbia blood agar

[Ref.: #66574]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65090]		growth	37 °C
[Ref.: #66574]	no	growth	4 °C
[Ref.: #66574]	no	growth	15 °C
[Ref.: #66574]		growth	22 °C
[Ref.: #66574]		growth	30 °C
[Ref.: #66574]		growth	35 °C
[Ref.: #66574]		growth	37 °C
[Ref.: #66574]		growth	42 °C
[Ref.: #66574]		growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66574]

Oxygen tolerance

facultative anaerobe

[Ref.: #66574]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66574]	amygdalin ChEBI	-	builds acid from
[Ref.: #66574]	arbutin ChEBI	+	builds acid from
[Ref.: #66574]	arginine ChEBI	-	hydrolysis
[Ref.: #66574]	cellobiose ChEBI	+	builds acid from
[Ref.: #66574]	cyclodextrin ChEBI	-	builds acid from
[Ref.: #66574]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66574]	D-arabitol ChEBI	+	builds acid from
[Ref.: #66574]	D-fructose ChEBI	+	builds acid from
[Ref.: #66574]	D-fucose ChEBI	-	builds acid from
[Ref.: #66574]	D-galactose ChEBI	+	builds acid from
[Ref.: #66574]	D-glucose ChEBI	+	builds acid from
[Ref.: #66574]	D-lyxose ChEBI	-	builds acid from
[Ref.: #66574]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66574]	D-mannitol ChEBI	+	builds acid from
[Ref.: #66574]	D-mannose ChEBI	+	builds acid from
[Ref.: #66574]	D-ribose ChEBI	-	builds acid from
[Ref.: #66574]	D-sorbitol ChEBI	+	builds acid from
[Ref.: #66574]	D-tagatose ChEBI	-	builds acid from
[Ref.: #66574]	D-xylose ChEBI	-	builds acid from
[Ref.: #66574]	erythritol ChEBI	-	builds acid from
[Ref.: #66574]	esculin ChEBI	+	builds acid from
[Ref.: #66574]	galactitol ChEBI	+	builds acid from
[Ref.: #66574]	gentiobiose ChEBI	-	builds acid from
[Ref.: #66574]	glycerol ChEBI	+	builds acid from
[Ref.: #66574]	glycogen ChEBI	-	builds acid from
[Ref.: #66574]	inulin ChEBI	+	builds acid from
[Ref.: #66574]	L-arabinose ChEBI	-	builds acid from
[Ref.: #66574]	L-arabitol ChEBI	+	builds acid from
[Ref.: #66574]	L-fucose ChEBI	-	builds acid from
[Ref.: #66574]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #66574]	L-sorbose ChEBI	+	builds acid from
[Ref.: #66574]	L-xylose ChEBI	-	builds acid from
[Ref.: #66574]	lactose ChEBI	+	builds acid from
[Ref.: #66574]	maltose ChEBI	+	builds acid from
[Ref.: #66574]	melezitose ChEBI	-	builds acid from
[Ref.: #66574]	melibiose ChEBI	-	builds acid from
[Ref.: #66574]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #66574]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #66574]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #66574]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66574]	myo-inositol ChEBI	+	builds acid from
[Ref.: #66574]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #66574]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66574]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #66574]	pullulan ChEBI	-	builds acid from
[Ref.: #66574]	raffinose ChEBI	-	builds acid from
[Ref.: #66574]	ribitol ChEBI	+	builds acid from
[Ref.: #66574]	salicin ChEBI	+	builds acid from
[Ref.: #66574]	sorbitol ChEBI	+	builds acid from
[Ref.: #66574]	starch ChEBI	-	builds acid from
[Ref.: #66574]	tagatose ChEBI	-	builds acid from
[Ref.: #66574]	turanose ChEBI	-	builds acid from
[Ref.: #66574]	urea ChEBI	-	hydrolysis
[Ref.: #66574]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66574]		acetoin ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66574]	acid phosphatase	-	3.1.3.2
[Ref.: #66574]	alkaline phosphatase	-	3.1.3.1
[Ref.: #66574]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #66574]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66574]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66574]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66574]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66574]	beta-galactosidase	+	3.2.1.23
[Ref.: #66574]	beta-glucosidase	-	3.2.1.21
[Ref.: #66574]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66574]	beta-mannosidase	-	3.2.1.25
[Ref.: #66574]	catalase	-	1.11.1.6
[Ref.: #66574]	cystine arylamidase	-	3.4.11.3
[Ref.: #66574]	esterase (C 4)	+
[Ref.: #66574]	esterase Lipase (C 8)	-
[Ref.: #66574]	glycyl tryptophan arylamidase	-
[Ref.: #66574]	leucine arylamidase	+	3.4.11.1
[Ref.: #66574]	lipase (C 14)	-
[Ref.: #66574]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66574]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #66574]	phenylalanine arylamidase	-
[Ref.: #66574]	pyroglutamic acid arylamidase	-
[Ref.: #66574]	trypsin	-	3.4.21.4
[Ref.: #66574]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65090]	Sample type/isolated from	respiratory tract of wild rodent Marmota himalayana
[Ref.: #65090]	Geographic location (country and/or sea, region)	Yushu, Qinghai Province
[Ref.: #65090]	Country	China
[Ref.: #65090]	Country ISO 3 Code	CHN
[Ref.: #65090]	Continent	Asia

[Ref.: #66574]	Sample type/isolated from	the respiratory tract of a wild marmot (Marmota himalayana) from Yushu
[Ref.: #66574]	Geographic location (country and/or sea, region)	Tibetan Autonomous Prefecture, Qinghai Province
[Ref.: #66574]	Country	China
[Ref.: #66574]	Country ISO 3 Code	CHN
[Ref.: #66574]	Continent	Asia
[Ref.: #66574]	Enrichment culture duration	2 days
[Ref.: #66574]	Enrichment culture temperature	37 ̊C
[Ref.: #66574]	Isolation/enrichment procedure	The trachea was opened, the inside wall scraped using sterile scissors and 30 mg of mucosal tissue were mixed with and fully ground in 1 ml of brain heart infusion (BHI) broth supplemented with 20 % fetal bovine serum (Gibco). Approximately 100 µl of the broth was spread onto Columbia agar containing 5 % defibrinated sheep blood (bio-Merieux) and incubated aerobically for 48 h at 37 °C under 5 % CO2.
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Rodentia (Other)
#Host Body-Site	#Oral cavity and airways	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66574]	Streptococcus respiraculi strain HTS25 16S ribosomal RNA gene, partial sequence	MF509297	1510	GenBank ENA	2021971 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptococcus respiraculi HTS25	GCA_003595525	complete	GenBank ENA	2021971 tax ID	*
[Ref.: #66792]	Streptococcus respiraculi HTS25	2825541525	complete	JGI IMG/M	2021971 tax ID	*

[Ref.: #65090]	GC-content	41.9 mol%
[Ref.: #66574]	GC-content	42.70 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Streptococcus respiraculi HTS25 NCBI: GCA_003595525.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Streptococcus respiraculi HTS25 NCBI: GCA_003595525.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.046	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.912	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	97.414	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	88.873	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.479	no

Availability in culture collections External links

[Ref.: #65090]

Culture collection no.

DSM 101998, CGMCC 1.15531

[Ref.: #111217]

SI-ID 401235

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Streptococcus respiraculi sp. nov. from Marmota himalayana (Himalayan marmot) respiratory tract.	Niu L, Hu S, Lu S, Lai XH, Yang J, Jin D, Rao L, Lu G, Xu J	Int J Syst Evol Microbiol	10.1099/ijsem.0.002806	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65090

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101998

#66574

Lina Niu, Shoukui Hu, Shan Lu, Xin-He Lai, Jing Yang, Dong Jin, Langyu Rao, Gang Lu, Jianguo Xu: Isolation and characterization of Streptococcus respiraculi sp. nov. from Marmota himalayana (Himalayan marmot) respiratory tract. IJSEM 68: 2082 - 2087 2018 ( DOI 10.1099/ijsem.0.002806 , PubMed 29717973 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111217

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401235.1 )

* These data were automatically processed and therefore are not curated

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