Strain identifier

BacDive ID: 158414

Type strain: Yes

Species: Streptococcus respiraculi

Strain Designation: HTS25

Strain history: <- L. Niu, Nat. Inst. for Communicable Disease Control and Prevention, Beijing, China; HTS25

NCBI tax ID(s): 2021971 (species)

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General

@ref: 65090

BacDive-ID: 158414

DSM-Number: 101998

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, coccus-shaped, colony-forming

description: Streptococcus respiraculi HTS25 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and builds arranged in pairs, short chains or small groups.

NCBI tax id

  • NCBI tax id: 2021971
  • Matching level: species

strain history

  • @ref: 65090
  • history: <- L. Niu, Nat. Inst. for Communicable Disease Control and Prevention, Beijing, China; HTS25

doi: 10.13145/bacdive158414.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Streptococcus
  • species: Streptococcus respiraculi
  • full scientific name: Streptococcus respiraculi Niu et al. 2018

@ref: 65090

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Streptococcus

species: Streptococcus respiraculi

full scientific name: Streptococcus respiraculi Niu et al. 2018

strain designation: HTS25

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
66574positive0.5-1.0 µmcoccus-shapedno
69480no90.479
69480positive90.046

colony morphology

  • @ref: 66574
  • colony size: 0.5-1 mm
  • colony color: non-pigmented
  • colony shape: circular
  • incubation period: 1 day
  • medium used: Columbia blood agar

multicellular morphology

  • @ref: 66574
  • forms multicellular complex: yes
  • complex name: arranged in pairs, short chains or small groups

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65090COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
66574Columbia blood agaryes

culture temp

@refgrowthtypetemperature
65090positivegrowth37
66574nogrowth4
66574nogrowth15
66574positivegrowth22
66574positivegrowth30
66574positivegrowth35
66574positivegrowth37
66574positivegrowth42
66574positivegrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 66574
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
66574no
69481no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
665746731melezitose-builds acid from
6657416443D-tagatose-builds acid from
6657416634raffinose-builds acid from
6657416899D-mannitol-builds acid from
6657416988D-ribose-builds acid from
6657417108D-arabinose-builds acid from
6657417113erythritol-builds acid from
6657417151xylitol-builds acid from
6657418287L-fucose-builds acid from
6657423456cyclodextrin-builds acid from
6657427613amygdalin-builds acid from
6657427941pullulan-builds acid from
6657428017starch-builds acid from
6657428053melibiose-builds acid from
6657428066gentiobiose-builds acid from
6657428087glycogen-builds acid from
6657428847D-fucose-builds acid from
6657430849L-arabinose-builds acid from
6657432528turanose-builds acid from
6657433954tagatose-builds acid from
6657462318D-lyxose-builds acid from
6657465327D-xylose-builds acid from
6657465328L-xylose-builds acid from
6657474863methyl beta-D-xylopyranoside-builds acid from
66574320055methyl beta-D-glucopyranoside-builds acid from
66574320061methyl alpha-D-glucopyranoside-builds acid from
66574potassium 2-dehydro-D-gluconate-builds acid from
665744853esculin+builds acid from
6657412936D-galactose+builds acid from
6657415443inulin+builds acid from
6657415824D-fructose+builds acid from
6657415963ribitol+builds acid from
6657416024D-mannose+builds acid from
6657416813galactitol+builds acid from
6657416899D-mannitol+builds acid from
6657417057cellobiose+builds acid from
6657417266L-sorbose+builds acid from
6657417268myo-inositol+builds acid from
6657417306maltose+builds acid from
6657417634D-glucose+builds acid from
6657417716lactose+builds acid from
6657417754glycerol+builds acid from
6657417814salicin+builds acid from
6657417924D-sorbitol+builds acid from
6657418305arbutin+builds acid from
6657418333D-arabitol+builds acid from
6657418403L-arabitol+builds acid from
6657430911sorbitol+builds acid from
6657443943methyl alpha-D-mannoside+builds acid from
6657459640N-acetylglucosamine+builds acid from
6657462345L-rhamnose+builds acid from
66574potassium 5-dehydro-D-gluconate+builds acid from
6657416199urea-hydrolysis
6657429016arginine-hydrolysis

metabolite production

  • @ref: 66574
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

enzymes

@refvalueactivityec
66574acid phosphatase-3.1.3.2
66574alkaline phosphatase-3.1.3.1
66574alpha-fucosidase-3.2.1.51
66574alpha-galactosidase-3.2.1.22
66574alpha-glucosidase-3.2.1.20
66574alpha-mannosidase-3.2.1.24
66574beta-glucosidase-3.2.1.21
66574beta-glucuronidase-3.2.1.31
66574beta-mannosidase-3.2.1.25
66574catalase-1.11.1.6
66574cystine arylamidase-3.4.11.3
66574esterase Lipase (C 8)-
66574glycyl tryptophan arylamidase-
66574lipase (C 14)-
66574N-acetyl-beta-glucosaminidase-3.2.1.52
66574naphthol-AS-BI-phosphohydrolase-
66574phenylalanine arylamidase-
66574pyroglutamic acid arylamidase-
66574trypsin-3.4.21.4
66574valine arylamidase-
66574alpha-chymotrypsin+3.4.21.1
66574beta-galactosidase+3.2.1.23
66574esterase (C 4)+
66574leucine arylamidase+3.4.11.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture durationenrichment culture temperatureisolation procedure
65090respiratory tract of wild rodent Marmota himalayanaYushu, Qinghai ProvinceChinaCHNAsia
66574the respiratory tract of a wild marmot (Marmota himalayana) from YushuTibetan Autonomous Prefecture, Qinghai ProvinceChinaCHNAsia2 days37The trachea was opened, the inside wall scraped using sterile scissors and 30 mg of mucosal tissue were mixed with and fully ground in 1 ml of brain heart infusion (BHI) broth supplemented with 20 % fetal bovine serum (Gibco). Approximately 100 µl of the broth was spread onto Columbia agar containing 5 % defibrinated sheep blood (bio-Merieux) and incubated aerobically for 48 h at 37 °C under 5 % CO2.

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Rodentia (Other)
#Host Body-Site#Oral cavity and airways

Sequence information

16S sequences

  • @ref: 66574
  • description: Streptococcus respiraculi strain HTS25 16S ribosomal RNA gene, partial sequence
  • accession: MF509297
  • length: 1510
  • database: nuccore
  • NCBI tax ID: 2021971

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptococcus respiraculi HTS25GCA_003595525completencbi2021971
66792Streptococcus respiraculi HTS252825541525completeimg2021971

GC content

@refGC-contentmethod
6509041.9
6657442.70genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes90.046no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no92.912yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.414yes
69480spore-formingspore-formingAbility to form endo- or exosporesno88.873no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96no
69480flagellatedmotile2+Ability to perform flagellated movementno90.479no

External links

@ref: 65090

culture collection no.: DSM 101998, CGMCC 1.15531

straininfo link

  • @ref: 111217
  • straininfo: 401235

literature

  • topic: Phylogeny
  • Pubmed-ID: 29717973
  • title: Isolation and characterization of Streptococcus respiraculi sp. nov. from Marmota himalayana (Himalayan marmot) respiratory tract.
  • authors: Niu L, Hu S, Lu S, Lai XH, Yang J, Jin D, Rao L, Lu G, Xu J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002806
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Genes, Bacterial, Marmota/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Respiratory System/*microbiology, Sequence Analysis, DNA, Streptococcus/*classification/genetics/isolation & purification
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65090Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101998Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101998)
66574Lina Niu, Shoukui Hu, Shan Lu, Xin-He Lai, Jing Yang, Dong Jin, Langyu Rao, Gang Lu, Jianguo XuIsolation and characterization of Streptococcus respiraculi sp. nov. from Marmota himalayana (Himalayan marmot) respiratory tract10.1099/ijsem.0.002806IJSEM 68: 2082-2087 201829717973
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111217Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401235.1