Name: Cohnella kolymensis 20.16T
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158415

Type strain:

Species: Cohnella kolymensis

Strain Designation: 20.16T

Culture col. no.: DSM 104983, VKM B-2846

Strain history: <- E. Kudryashova; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences., Pushchino, Moscow region, RUSSIA; 20.16T<-E. B. Kudryashova, E. V. Ariskina; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

NCBI tax ID(s): 1590652 (species)

SI-ID 404341

DSM 104983

Special Collections

Section

Cohnella kolymensis 20.16T is a facultative anaerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from Permafrost sampling.

colony-forming
Gram-positive
rod-shaped
spore-forming
chemoorganotroph
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella kolymensis**
	Full Scientific Name (LPSN)	Cohnella kolymensis Kudryashova et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66651]	Gram stain	positive

[Ref.: #66651]	Cell length	1.0-3.5 µm

[Ref.: #66651]	Cell width	0.3-0.5 µm

[Ref.: #66651]	Cell shape	rod-shaped

[Ref.: #66651]	Motility	no

[Ref.: #66651]	Colony size	1-1.5 mm
[Ref.: #66651]	Colony shape	circular
[Ref.: #66651]	Incubation period	2 days
[Ref.: #66651]	Cultivation medium used	R2A medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65091]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #65091]

Culture medium growth

[Ref.: #65091]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65091]

Culture medium composition

expand / minimize

[Ref.: #66651]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66651]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65091]		growth	30 °C
[Ref.: #66651]	no	growth	4 °C
[Ref.: #66651]		optimum	28 °C
[Ref.: #66651]	no	growth	45 °C
[Ref.: #66651]		growth	6-43 °C

	pH	Kind of pH		pH
[Ref.: #66651]			optimum	6-7
[Ref.: #66651]			growth	4.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66651]

Oxygen tolerance

facultative anaerobe

[Ref.: #66651]

Nutrition type

chemoorganotroph

[Ref.: #66651]	Spore description	oval and positioned terminally or subterminally in swollen sporangia
[Ref.: #66651]	Type of spore	endospore
[Ref.: #66651]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66651]		NaCl		growth	0-0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66651]	amygdalin ChEBI	+	hydrolysis
[Ref.: #66651]	arbutin ChEBI	-	builds acid from
[Ref.: #66651]	casein	-	hydrolysis
[Ref.: #66651]	cellobiose ChEBI	+	builds acid from
[Ref.: #66651]	cellulose ChEBI	-	assimilation
[Ref.: #66651]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66651]	D-arabitol ChEBI	-	builds acid from
[Ref.: #66651]	D-fructose ChEBI	+	builds acid from
[Ref.: #66651]	D-fucose ChEBI	-	builds acid from
[Ref.: #66651]	D-galactose ChEBI	-	builds acid from
[Ref.: #66651]	D-glucose ChEBI	+	builds acid from
[Ref.: #66651]	D-lyxose ChEBI	+	builds acid from
[Ref.: #66651]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66651]	D-mannose ChEBI	+	builds acid from
[Ref.: #66651]	D-ribose ChEBI	-	builds acid from
[Ref.: #66651]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66651]	D-tagatose ChEBI	+	builds acid from
[Ref.: #66651]	D-xylose ChEBI	+	builds acid from
[Ref.: #66651]	erythritol ChEBI	-	builds acid from
[Ref.: #66651]	esculin ChEBI	+	hydrolysis
[Ref.: #66651]	galactitol ChEBI	-	builds acid from
[Ref.: #66651]	gelatin ChEBI	-	hydrolysis
[Ref.: #66651]	gentiobiose ChEBI	-	builds acid from
[Ref.: #66651]	glycerol ChEBI	-	builds acid from
[Ref.: #66651]	glycogen ChEBI	+	builds acid from
[Ref.: #66651]	inulin ChEBI	-	builds acid from
[Ref.: #66651]	L-arabinose ChEBI	+	builds acid from
[Ref.: #66651]	L-arabitol ChEBI	-	builds acid from
[Ref.: #66651]	L-fucose ChEBI	-	builds acid from
[Ref.: #66651]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66651]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66651]	L-xylose ChEBI	-	builds acid from
[Ref.: #66651]	lactose ChEBI	-	builds acid from
[Ref.: #66651]	maltose ChEBI	+	builds acid from
[Ref.: #66651]	melezitose ChEBI	-	builds acid from
[Ref.: #66651]	melibiose ChEBI	-	builds acid from
[Ref.: #66651]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #66651]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #66651]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66651]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66651]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #66651]	nitrate ChEBI	-	reduction
[Ref.: #66651]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66651]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #66651]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66651]	raffinose ChEBI	-	builds acid from
[Ref.: #66651]	ribitol ChEBI	-	builds acid from
[Ref.: #66651]	salicin ChEBI	+	builds acid from
[Ref.: #66651]	starch ChEBI	-	builds acid from
[Ref.: #66651]	starch ChEBI	-	hydrolysis
[Ref.: #66651]	sucrose ChEBI	+	builds acid from
[Ref.: #66651]	trehalose ChEBI	+	builds acid from
[Ref.: #66651]	turanose ChEBI	+	builds acid from
[Ref.: #66651]	tween 80 ChEBI	+	hydrolysis
[Ref.: #66651]	xylan ChEBI	+/-	assimilation
[Ref.: #66651]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #66651]	ampicillin ChEBI	yes	30	µg (disc)
[Ref.: #66651]	chloramphenicol ChEBI	yes	10	µg (disc)
[Ref.: #66651]	kanamycin ChEBI	yes	5	µg (disc)
[Ref.: #66651]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #66651]	rifampicin ChEBI	yes	30	µg (disc)
[Ref.: #66651]	tetracycline ChEBI	yes	10	µg (disc)

	Metabolite	Production
[Ref.: #66651]	acetoin ChEBI	yes
[Ref.: #66651]	hydrogen sulfide ChEBI	no
[Ref.: #66651]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #66651]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66651]	acid phosphatase	-	3.1.3.2
[Ref.: #66651]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66651]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66651]	alpha-fucosidase	+	3.2.1.51
[Ref.: #66651]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66651]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66651]	alpha-mannosidase	+	3.2.1.24
[Ref.: #66651]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66651]	beta-galactosidase	-	3.2.1.23
[Ref.: #66651]	beta-galactosidase	+	3.2.1.23
[Ref.: #66651]	beta-glucosidase	+	3.2.1.21
[Ref.: #66651]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66651]	catalase	+	1.11.1.6
[Ref.: #66651]	cystine arylamidase	-	3.4.11.3
[Ref.: #66651]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66651]	esterase (C 4)	+
[Ref.: #66651]	esterase Lipase (C 8)	+
[Ref.: #66651]	leucine arylamidase	-	3.4.11.1
[Ref.: #66651]	lipase (C 14)	-
[Ref.: #66651]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66651]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66651]	trypsin	-	3.4.21.4
[Ref.: #66651]	urease	-	3.5.1.5
[Ref.: #66651]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65091]	Sample type/isolated from	Permafrost sampling
[Ref.: #65091]	Geographic location (country and/or sea, region)	The ground surface of high alas, the outcrops area at the Duvaniy Yar site on the Kolyma river, East Siberia (N 68° 38' 0.28", E 159° 4' 43.68")
[Ref.: #65091]	Country	Russia
[Ref.: #65091]	Country ISO 3 Code	RUS
[Ref.: #65091]	Continent	Asia

[Ref.: #66651]	Sample type/isolated from	permafrost sediment
[Ref.: #66651]	Geographic location (country and/or sea, region)	Duvaniy Yar, Kolyma River, East Siberia
[Ref.: #66651]	Country	Russia
[Ref.: #66651]	Country ISO 3 Code	RUS
[Ref.: #66651]	Continent	Asia
[Ref.: #66651]	Geographic location	68.6334°/159.0790°
[Ref.: #66651]	Enrichment culture	fivefold diluted nutrient agar
[Ref.: #66651]	Enrichment culture duration	30 days
[Ref.: #66651]	Enrichment culture temperature	24 ̊C
[Ref.: #66651]	Isolation/enrichment procedure	cooled (4°C) saline solution (0.85% NaCl) supplemented with 0.1% sodium pyruvate was added to permafrost sample and vortexed. The suspension was plated on the fivefold diluted nutrient agar.
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Permafrost

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66651]	Paenibacillaceae bacterium VKM B-2846 16S ribosomal RNA gene, partial sequence	KT265226	1504	GenBank ENA	1815578 tax ID

[Ref.: #66651]

GC-content

50.50 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65091]

Culture collection no.

DSM 104983, VKM B-2846

[Ref.: #111218]

SI-ID 404341

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost.	Kudryashova EB, Karlyshev AV, Ariskina EV, Streshinskaya GM, Vinokurova NG, Kopitsyn DS, Evtushenko LI	Int J Syst Evol Microbiol	10.1099/ijsem.0.002919	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65091

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104983

#66651

Ekaterina B. Kudryashova, Andrey V. Karlyshev, Elena V. Ariskina, Galina M. Streshinskaya, Nataliy G. Vinokurova, Dmitry S. Kopitsyn and Lyudmila I. Evtushenko: Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost. IJSEM 68: 2912 - 2917 2018 ( DOI 10.1099/ijsem.0.002919 , PubMed 30016228 )

#111218

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404341.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Cohnella kolymensis in external resources:
SILVA
BRENDA
PANGAEA
GBIF