Name: Cohnella kolymensis 20.16T
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158415

Type strain:

Species: Cohnella kolymensis

Strain Designation: 20.16T

Culture col. no.: DSM 104983, VKM B-2846

Strain history: <- E. Kudryashova; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences., Pushchino, Moscow region, RUSSIA; 20.16T<-E. B. Kudryashova, E. V. Ariskina; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

NCBI tax ID(s): 1590652 (species)

SI-ID 404341

DSM 104983

Section

Cohnella kolymensis 20.16T is a facultative anaerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from Permafrost sampling.

colony-forming
Gram-positive
rod-shaped
spore-forming
chemoorganotroph
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella kolymensis**
	Full Scientific Name (LPSN)	Cohnella kolymensis Kudryashova et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66651]	Gram stain	positive

[Ref.: #66651]	Cell length	1.0-3.5 µm

[Ref.: #66651]	Cell width	0.3-0.5 µm

[Ref.: #66651]	Cell shape	rod-shaped

[Ref.: #66651]	Motility	no

[Ref.: #66651]	Colony size	1-1.5 mm
[Ref.: #66651]	Colony shape	circular
[Ref.: #66651]	Incubation period	2 days
[Ref.: #66651]	Cultivation medium used	R2A medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65091]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #65091]

Culture medium growth

[Ref.: #65091]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65091]

Culture medium composition

expand / minimize

[Ref.: #66651]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #66651]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #65091]		growth	30 °C
[Ref.: #66651]	no	growth	4 °C
[Ref.: #66651]		optimum	28 °C
[Ref.: #66651]	no	growth	45 °C
[Ref.: #66651]		growth	6-43 °C

[Ref.: #65091]	Temperature range	mesophilic
[Ref.: #66651]	Temperature range	psychrophilic
[Ref.: #66651]	Temperature range	mesophilic
[Ref.: #66651]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #66651]			optimum	6-7
[Ref.: #66651]			growth	4.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66651]

Oxygen tolerance

facultative anaerobe

[Ref.: #66651]

Nutrition type

chemoorganotroph

[Ref.: #66651]	Spore description	oval and positioned terminally or subterminally in swollen sporangia
[Ref.: #66651]	Type of spore	endospore
[Ref.: #66651]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66651]		NaCl		growth	0-0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66651]	amygdalin ChEBI	+	hydrolysis
[Ref.: #66651]	arbutin ChEBI	-	builds acid from
[Ref.: #66651]	casein	-	hydrolysis
[Ref.: #66651]	cellobiose ChEBI	+	builds acid from
[Ref.: #66651]	cellulose ChEBI	-	assimilation
[Ref.: #66651]	D-arabinose ChEBI	-	builds acid from
[Ref.: #66651]	D-arabitol ChEBI	-	builds acid from
[Ref.: #66651]	D-fructose ChEBI	+	builds acid from
[Ref.: #66651]	D-fucose ChEBI	-	builds acid from
[Ref.: #66651]	D-galactose ChEBI	-	builds acid from
[Ref.: #66651]	D-glucose ChEBI	+	builds acid from
[Ref.: #66651]	D-lyxose ChEBI	+	builds acid from
[Ref.: #66651]	D-mannitol ChEBI	-	builds acid from
[Ref.: #66651]	D-mannose ChEBI	+	builds acid from
[Ref.: #66651]	D-ribose ChEBI	-	builds acid from
[Ref.: #66651]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #66651]	D-tagatose ChEBI	+	builds acid from
[Ref.: #66651]	D-xylose ChEBI	+	builds acid from
[Ref.: #66651]	erythritol ChEBI	-	builds acid from
[Ref.: #66651]	esculin ChEBI	+	hydrolysis
[Ref.: #66651]	galactitol ChEBI	-	builds acid from
[Ref.: #66651]	gelatin ChEBI	-	hydrolysis
[Ref.: #66651]	gentiobiose ChEBI	-	builds acid from
[Ref.: #66651]	glycerol ChEBI	-	builds acid from
[Ref.: #66651]	glycogen ChEBI	+	builds acid from
[Ref.: #66651]	inulin ChEBI	-	builds acid from
[Ref.: #66651]	L-arabinose ChEBI	+	builds acid from
[Ref.: #66651]	L-arabitol ChEBI	-	builds acid from
[Ref.: #66651]	L-fucose ChEBI	-	builds acid from
[Ref.: #66651]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66651]	L-sorbose ChEBI	-	builds acid from
[Ref.: #66651]	L-xylose ChEBI	-	builds acid from
[Ref.: #66651]	lactose ChEBI	-	builds acid from
[Ref.: #66651]	maltose ChEBI	+	builds acid from
[Ref.: #66651]	melezitose ChEBI	-	builds acid from
[Ref.: #66651]	melibiose ChEBI	-	builds acid from
[Ref.: #66651]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #66651]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #66651]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #66651]	myo-inositol ChEBI	-	builds acid from
[Ref.: #66651]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #66651]	nitrate ChEBI	-	reduction
[Ref.: #66651]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #66651]	potassium 5-dehydro-D-gluconate	-	builds acid from
[Ref.: #66651]	potassium gluconate ChEBI	-	builds acid from
[Ref.: #66651]	raffinose ChEBI	-	builds acid from
[Ref.: #66651]	ribitol ChEBI	-	builds acid from
[Ref.: #66651]	salicin ChEBI	+	builds acid from
[Ref.: #66651]	starch ChEBI	-	builds acid from
[Ref.: #66651]	starch ChEBI	-	hydrolysis
[Ref.: #66651]	sucrose ChEBI	+	builds acid from
[Ref.: #66651]	trehalose ChEBI	+	builds acid from
[Ref.: #66651]	turanose ChEBI	+	builds acid from
[Ref.: #66651]	tween 80 ChEBI	+	hydrolysis
[Ref.: #66651]	xylan ChEBI	+/-	assimilation
[Ref.: #66651]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #66651]	ampicillin ChEBI	yes	30	µg (disc)
[Ref.: #66651]	chloramphenicol ChEBI	yes	10	µg (disc)
[Ref.: #66651]	kanamycin ChEBI	yes	5	µg (disc)
[Ref.: #66651]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #66651]	rifampicin ChEBI	yes	30	µg (disc)
[Ref.: #66651]	tetracycline ChEBI	yes	10	µg (disc)

	Metabolite	Production
[Ref.: #66651]	acetoin ChEBI	yes
[Ref.: #66651]	hydrogen sulfide ChEBI	no
[Ref.: #66651]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #66651]		acetoin ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #66651]	acid phosphatase	-	3.1.3.2
[Ref.: #66651]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66651]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66651]	alpha-fucosidase	+	3.2.1.51
[Ref.: #66651]	alpha-galactosidase	+	3.2.1.22
[Ref.: #66651]	alpha-glucosidase	+	3.2.1.20
[Ref.: #66651]	alpha-mannosidase	+	3.2.1.24
[Ref.: #66651]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66651]	beta-galactosidase	-	3.2.1.23
[Ref.: #66651]	beta-galactosidase	+	3.2.1.23
[Ref.: #66651]	beta-glucosidase	+	3.2.1.21
[Ref.: #66651]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66651]	catalase	+	1.11.1.6
[Ref.: #66651]	cystine arylamidase	-	3.4.11.3
[Ref.: #66651]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66651]	esterase (C 4)	+
[Ref.: #66651]	esterase Lipase (C 8)	+
[Ref.: #66651]	leucine arylamidase	-	3.4.11.1
[Ref.: #66651]	lipase (C 14)	-
[Ref.: #66651]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #66651]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66651]	trypsin	-	3.4.21.4
[Ref.: #66651]	urease	-	3.5.1.5
[Ref.: #66651]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65091]	Sample type/isolated from	Permafrost sampling
[Ref.: #65091]	Geographic location (country and/or sea, region)	The ground surface of high alas, the outcrops area at the Duvaniy Yar site on the Kolyma river, East Siberia (N 68° 38' 0.28", E 159° 4' 43.68")
[Ref.: #65091]	Country	Russia
[Ref.: #65091]	Country ISO 3 Code	RUS
[Ref.: #65091]	Continent	Asia

[Ref.: #66651]	Sample type/isolated from	permafrost sediment
[Ref.: #66651]	Geographic location (country and/or sea, region)	Duvaniy Yar, Kolyma River, East Siberia
[Ref.: #66651]	Country	Russia
[Ref.: #66651]	Country ISO 3 Code	RUS
[Ref.: #66651]	Continent	Asia
[Ref.: #66651]	Geographic location	68.6334°/159.0790°
[Ref.: #66651]	Enrichment culture	fivefold diluted nutrient agar
[Ref.: #66651]	Enrichment culture duration	30 days
[Ref.: #66651]	Enrichment culture temperature	24 ̊C
[Ref.: #66651]	Isolation/enrichment procedure	cooled (4°C) saline solution (0.85% NaCl) supplemented with 0.1% sodium pyruvate was added to permafrost sample and vortexed. The suspension was plated on the fivefold diluted nutrient agar.
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Permafrost

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66651]	Paenibacillaceae bacterium VKM B-2846 16S ribosomal RNA gene, partial sequence	KT265226	1504	ENA	1815578 tax ID

[Ref.: #66651]

GC-content

50.50 mol%

genome sequence analysis

Availability in culture collections External links

[Ref.: #65091]

Culture collection no.

DSM 104983, VKM B-2846

[Ref.: #111218]

SI-ID 404341

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost.	Kudryashova EB, Karlyshev AV, Ariskina EV, Streshinskaya GM, Vinokurova NG, Kopitsyn DS, Evtushenko LI	Int J Syst Evol Microbiol	10.1099/ijsem.0.002919	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65091

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104983

#66651

Ekaterina B. Kudryashova, Andrey V. Karlyshev, Elena V. Ariskina, Galina M. Streshinskaya, Nataliy G. Vinokurova, Dmitry S. Kopitsyn and Lyudmila I. Evtushenko: Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost. IJSEM 68: 2912 - 2917 2018 ( DOI 10.1099/ijsem.0.002919 , PubMed 30016228 )

#111218

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404341.1 )

* These data were automatically processed and therefore are not curated

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