Cohnella kolymensis (DSM 104983, VKM B-2846, 20.16T)

Name: Cohnella kolymensis (DSM 104983, VKM B-2846, 20.16T)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158415

Type strain

Strain Designation 20.16T

Culture col. no. DSM 104983 VKM B-2846 20.16T

NCBI tax ID(s) 1590652

Special Collections DSMZ Literature strains

History DSMZ <- E. Kudryashova; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences., Pushchino, Moscow region, RUSSIA; 20.16T<-E. B. Kudryashova, E. V. Ariskina; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

Links

Cohnella kolymensis 20.16T is a facultative anaerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from Permafrost sampling.

spore-forming Gram-positive rod-shaped colony-forming facultative anaerobe chemoorganotroph genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 404341

VKM B-2846,DSM 104983

@ref 20215

Last LPSN update 2026-05-29 08:43:57

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Paenibacillaceae

Genus Cohnella

Species Cohnella kolymensis

Full scientific name Cohnella kolymensis Kudryashova et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Confidence
66651	positive	1.0-3.5 µm	0.3-0.5 µm	rod-shaped
125439						92.772

Colony morphology

@ref	Colony size	Colony shape	Incubation period	Medium used
66651	1-1.5 mm	circular	2 days	R2A medium

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65091	R2A MEDIUM (DSMZ Medium 830)		Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
66651	Reasoner's 2A agar (R2A)

Culture temp

@ref	Growth	Type	Temperature (°C)
65091	positive	growth	30
66651	negative	growth	4
66651	positive	optimum	28
66651	negative	growth	45
66651	positive	growth	6-43

Culture pH

@ref	Ability	Type	PH	PH range
66651	positive	optimum	6-7
66651	positive	growth	4.0-8.0	acidophile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
66651	facultative anaerobe
125439	obligate aerobe	97.003

Nutrition type

66651

Typechemoorganotroph

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
66651	oval and positioned terminally or subterminally in swollen sporangia	endospore
125439				97.558

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66651	NaCl	positive	growth	0-0.5 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66651	27613 ChEBI	amygdalin	+	hydrolysis
66651	18305 ChEBI	arbutin	-	builds acid from
66651		casein	-	hydrolysis
66651	17057 ChEBI	cellobiose	+	builds acid from
66651	62968 ChEBI	cellulose	-	assimilation
66651	17108 ChEBI	D-arabinose	-	builds acid from
66651	18333 ChEBI	D-arabitol	-	builds acid from
66651	15824 ChEBI	D-fructose	+	builds acid from
66651	28847 ChEBI	D-fucose	-	builds acid from
66651	12936 ChEBI	D-galactose	-	builds acid from
66651	17634 ChEBI	D-glucose	+	builds acid from
66651	62318 ChEBI	D-lyxose	+	builds acid from
66651	16899 ChEBI	D-mannitol	-	builds acid from
66651	16024 ChEBI	D-mannose	+	builds acid from
66651	16988 ChEBI	D-ribose	-	builds acid from
66651	17924 ChEBI	D-sorbitol	-	builds acid from
66651	16443 ChEBI	D-tagatose	+	builds acid from
66651	65327 ChEBI	D-xylose	+	builds acid from
66651	17113 ChEBI	erythritol	-	builds acid from
66651	4853 ChEBI	esculin	+	hydrolysis
66651	16813 ChEBI	galactitol	-	builds acid from
66651	5291 ChEBI	gelatin	-	hydrolysis
66651	28066 ChEBI	gentiobiose	-	builds acid from
66651	17754 ChEBI	glycerol	-	builds acid from
66651	28087 ChEBI	glycogen	+	builds acid from
66651	15443 ChEBI	inulin	-	builds acid from
66651	30849 ChEBI	L-arabinose	+	builds acid from
66651	18403 ChEBI	L-arabitol	-	builds acid from
66651	18287 ChEBI	L-fucose	-	builds acid from
66651	62345 ChEBI	L-rhamnose	-	builds acid from
66651	17266 ChEBI	L-sorbose	-	builds acid from
66651	65328 ChEBI	L-xylose	-	builds acid from
66651	17716 ChEBI	lactose	-	builds acid from
66651	17306 ChEBI	maltose	+	builds acid from
66651	6731 ChEBI	melezitose	-	builds acid from
66651	28053 ChEBI	melibiose	-	builds acid from
66651	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from
66651	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
66651	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
66651	17268 ChEBI	myo-inositol	-	builds acid from
66651	59640 ChEBI	N-acetylglucosamine	+	builds acid from
66651	17632 ChEBI	nitrate	-	reduction
66651		potassium 2-dehydro-D-gluconate	-	builds acid from
66651		potassium 5-dehydro-D-gluconate	-	builds acid from
66651	32032 ChEBI	potassium gluconate	-	builds acid from
66651	16634 ChEBI	raffinose	-	builds acid from
66651	15963 ChEBI	ribitol	-	builds acid from
66651	17814 ChEBI	salicin	+	builds acid from
66651	28017 ChEBI	starch	-	builds acid from
66651	28017 ChEBI	starch	-	hydrolysis
66651	17992 ChEBI	sucrose	+	builds acid from
66651	27082 ChEBI	trehalose	+	builds acid from
66651	32528 ChEBI	turanose	+	builds acid from
66651	53426 ChEBI	tween 80	+	hydrolysis
66651	37166 ChEBI	xylan	+/-	assimilation
66651	17151 ChEBI	xylitol	-	builds acid from

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.
66651	28971	ampicillin	30 µg (disc)
66651	17698	chloramphenicol	10 µg (disc)
66651	6104	kanamycin	5 µg (disc)
66651	17334	penicillin	10 Unit (disc)
66651	28077	rifampicin	30 µg (disc)
66651	27902	tetracycline	10 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite
66651	15688 ChEBI	acetoin
66651	16136 ChEBI	hydrogen sulfide
66651	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
66651	15688 ChEBI	acetoin	+

Enzymes

@ref	Value	Activity	Ec
66651	acid phosphatase	-	3.1.3.2
66651	alkaline phosphatase	+	3.1.3.1
66651	alpha-chymotrypsin	-	3.4.21.1
66651	alpha-fucosidase	+	3.2.1.51
66651	alpha-galactosidase	+	3.2.1.22
66651	alpha-glucosidase	+	3.2.1.20
66651	alpha-mannosidase	+	3.2.1.24
66651	arginine dihydrolase	-	3.5.3.6
66651	beta-galactosidase	-	3.2.1.23
66651	beta-galactosidase	+	3.2.1.23
66651	beta-glucosidase	+	3.2.1.21
66651	beta-glucuronidase	-	3.2.1.31
66651	catalase	+	1.11.1.6
66651	cystine arylamidase	-	3.4.11.3
66651	cytochrome oxidase	+	1.9.3.1
66651	esterase (C 4)	+
66651	esterase Lipase (C 8)	+
66651	leucine arylamidase	-	3.4.11.1
66651	lipase (C 14)	-
66651	N-acetyl-beta-glucosaminidase	+	3.2.1.52
66651	naphthol-AS-BI-phosphohydrolase	+
66651	trypsin	-	3.4.21.4
66651	urease	-	3.5.1.5
66651	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Permafrost

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
65091	Permafrost sampling	The ground surface of high alas, the outcrops area at the Duvaniy Yar site on the Kolyma river, East Siberia (N 68° 38' 0.28", E 159° 4' 43.68")	Russia	RUS	Asia
66651	permafrost sediment	Duvaniy Yar, Kolyma River, East Siberia	Russia	RUS	Asia	68.6334	159.079 68.6334/159.079	fivefold diluted nutrient agar	30 days	24	cooled (4°C) saline solution (0.85% NaCl) supplemented with 0.1% sodium pyruvate was added to permafrost sample and vortexed. The suspension was plated on the fivefold diluted nutrient agar.

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM82946v1 assembly for Cohnella kolymensis VKM B-2846	contig	GCA_000829465	1590652.3	2630968607	1590652	59.34

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66651	Paenibacillaceae bacterium VKM B-2846 16S ribosomal RNA gene, partial sequence	KT265226	1504		1815578

GC content

@ref	GC-content (mol%)	Method
66651	50.50	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM82946v1 assembly for Cohnella kolymensis VKM B-2846
NCBI: GCA_000829465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	variable	78.42	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.00	no
125439	motility	BacteriaNetⓘ	yes	92.77	no
125439	spore_formation	BacteriaNetⓘ	yes	97.56	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequence of "Cohnella kolymensis" B-2846.	Karlyshev AV, Kudryashova EB, Ariskina EV.	Genome Announc	10.1128/genomea.01587-15	2016
Phylogeny	Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost.	Kudryashova EB, Karlyshev AV, Ariskina EV, Streshinskaya GM, Vinokurova NG, Kopitsyn DS, Evtushenko LI	Int J Syst Evol Microbiol	10.1099/ijsem.0.002919	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65091	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104983
#66651	Ekaterina B. Kudryashova, Andrey V. Karlyshev, Elena V. Ariskina, Galina M. Streshinskaya, Nataliy G. Vinokurova, Dmitry S. Kopitsyn and Lyudmila I. Evtushenko: Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost. IJSEM 68: 2912 - 2917 2018 ( DOI 10.1099/ijsem.0.002919 , PubMed 30016228 )
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Cohnella kolymensis (DSM 104983, VKM B-2846, 20.16T)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_000829465

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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