Strain identifier

BacDive ID: 158415

Type strain: Yes

Species: Cohnella kolymensis

Strain Designation: 20.16T

Strain history: <- E. Kudryashova; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences., Pushchino, Moscow region, RUSSIA; 20.16T<-E. B. Kudryashova, E. V. Ariskina; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

NCBI tax ID(s): 1590652 (species)

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General

@ref: 65091

BacDive-ID: 158415

DSM-Number: 104983

keywords: 16S sequence, Bacteria, facultative anaerobe, chemoorganotroph, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Cohnella kolymensis 20.16T is a facultative anaerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from Permafrost sampling.

NCBI tax id

  • NCBI tax id: 1590652
  • Matching level: species

strain history

  • @ref: 65091
  • history: <- E. Kudryashova; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences., Pushchino, Moscow region, RUSSIA; 20.16T<-E. B. Kudryashova, E. V. Ariskina; G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

doi: 10.13145/bacdive158415.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Cohnella
  • species: Cohnella kolymensis
  • full scientific name: Cohnella kolymensis Kudryashova et al. 2018

@ref: 65091

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Cohnella

species: Cohnella kolymensis

full scientific name: Cohnella kolymensis Kudryashova et al. 2018

strain designation: 20.16T

type strain: yes

Morphology

cell morphology

  • @ref: 66651
  • gram stain: positive
  • cell length: 1.0-3.5 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66651
  • colony size: 1-1.5 mm
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65091R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
66651Reasoner's 2A agar (R2A)yes

culture temp

@refgrowthtypetemperature
65091positivegrowth30
66651nogrowth4
66651positiveoptimum28
66651nogrowth45
66651positivegrowth6-43

culture pH

@refabilitytypepHPH range
66651positivegrowth4.0-8.0acidophile
66651positiveoptimum6-7

Physiology and metabolism

oxygen tolerance

  • @ref: 66651
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 66651
  • type: chemoorganotroph

spore formation

  • @ref: 66651
  • spore description: oval and positioned terminally or subterminally in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

  • @ref: 66651
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-0.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6665162968cellulose-assimilation
6665137166xylan+/-assimilation
666516731melezitose-builds acid from
6665112936D-galactose-builds acid from
6665115443inulin-builds acid from
6665115963ribitol-builds acid from
6665116634raffinose-builds acid from
6665116813galactitol-builds acid from
6665116899D-mannitol-builds acid from
6665116988D-ribose-builds acid from
6665117108D-arabinose-builds acid from
6665117113erythritol-builds acid from
6665117151xylitol-builds acid from
6665117266L-sorbose-builds acid from
6665117268myo-inositol-builds acid from
6665117716lactose-builds acid from
6665117754glycerol-builds acid from
6665117924D-sorbitol-builds acid from
6665118287L-fucose-builds acid from
6665118305arbutin-builds acid from
6665118333D-arabitol-builds acid from
6665118403L-arabitol-builds acid from
6665128017starch-builds acid from
6665128053melibiose-builds acid from
6665128066gentiobiose-builds acid from
6665128847D-fucose-builds acid from
6665132032potassium gluconate-builds acid from
6665143943methyl alpha-D-mannoside-builds acid from
6665162345L-rhamnose-builds acid from
6665165328L-xylose-builds acid from
6665174863methyl beta-D-xylopyranoside-builds acid from
66651potassium 2-dehydro-D-gluconate-builds acid from
66651potassium 5-dehydro-D-gluconate-builds acid from
6665115824D-fructose+builds acid from
6665116024D-mannose+builds acid from
6665116443D-tagatose+builds acid from
6665117057cellobiose+builds acid from
6665117306maltose+builds acid from
6665117634D-glucose+builds acid from
6665117814salicin+builds acid from
6665117992sucrose+builds acid from
6665127082trehalose+builds acid from
6665128087glycogen+builds acid from
6665130849L-arabinose+builds acid from
6665132528turanose+builds acid from
6665159640N-acetylglucosamine+builds acid from
6665162318D-lyxose+builds acid from
6665165327D-xylose+builds acid from
66651320061methyl alpha-D-glucopyranoside+builds acid from
666515291gelatin-hydrolysis
6665128017starch-hydrolysis
66651casein-hydrolysis
666514853esculin+hydrolysis
6665127613amygdalin+hydrolysis
6665153426tween 80+hydrolysis
6665117632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6665128971ampicillinyesyes30 µg (disc)
6665117698chloramphenicolyesyes10 µg (disc)
666516104kanamycinyesyes5 µg (disc)
6665117334penicillinyesyes10 Unit (disc)
6665127902tetracyclineyesyes10 µg (disc)
6665128077rifampicinyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6665115688acetoinyes
6665116136hydrogen sulfideno
6665135581indoleno

metabolite tests

  • @ref: 66651
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
66651acid phosphatase-3.1.3.2
66651alpha-chymotrypsin-3.4.21.1
66651arginine dihydrolase-3.5.3.6
66651beta-galactosidase-3.2.1.23
66651beta-glucuronidase-3.2.1.31
66651cystine arylamidase-3.4.11.3
66651leucine arylamidase-3.4.11.1
66651lipase (C 14)-
66651trypsin-3.4.21.4
66651urease-3.5.1.5
66651valine arylamidase-
66651alkaline phosphatase+3.1.3.1
66651alpha-fucosidase+3.2.1.51
66651alpha-galactosidase+3.2.1.22
66651alpha-glucosidase+3.2.1.20
66651alpha-mannosidase+3.2.1.24
66651beta-galactosidase+3.2.1.23
66651beta-glucosidase+3.2.1.21
66651catalase+1.11.1.6
66651cytochrome oxidase+1.9.3.1
66651esterase (C 4)+
66651esterase Lipase (C 8)+
66651N-acetyl-beta-glucosaminidase+3.2.1.52
66651naphthol-AS-BI-phosphohydrolase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65091Permafrost samplingThe ground surface of high alas, the outcrops area at the Duvaniy Yar site on the Kolyma river, East Siberia (N 68° 38' 0.28", E 159° 4' 43.68")RussiaRUSAsia
66651permafrost sedimentDuvaniy Yar, Kolyma River, East SiberiaRussiaRUSAsia68.6334159.079fivefold diluted nutrient agar30 days24cooled (4°C) saline solution (0.85% NaCl) supplemented with 0.1% sodium pyruvate was added to permafrost sample and vortexed. The suspension was plated on the fivefold diluted nutrient agar.

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Permafrost

Sequence information

16S sequences

  • @ref: 66651
  • description: Paenibacillaceae bacterium VKM B-2846 16S ribosomal RNA gene, partial sequence
  • accession: KT265226
  • length: 1504
  • database: nuccore
  • NCBI tax ID: 1815578

GC content

  • @ref: 66651
  • GC-content: 50.50
  • method: genome sequence analysis

External links

@ref: 65091

culture collection no.: DSM 104983, VKM B-2846

straininfo link

  • @ref: 111218
  • straininfo: 404341

literature

  • topic: Phylogeny
  • Pubmed-ID: 30016228
  • title: Cohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost.
  • authors: Kudryashova EB, Karlyshev AV, Ariskina EV, Streshinskaya GM, Vinokurova NG, Kopitsyn DS, Evtushenko LI
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002919
  • year: 2018
  • mesh: Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Peptidoglycan/chemistry, Permafrost/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Siberia, *Soil Microbiology, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65091Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104983Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104983)
66651Ekaterina B. Kudryashova, Andrey V. Karlyshev, Elena V. Ariskina, Galina M. Streshinskaya, Nataliy G. Vinokurova, Dmitry S. Kopitsyn and Lyudmila I. EvtushenkoCohnella kolymensis sp. nov., a novel bacillus isolated from Siberian permafrost10.1099/ijsem.0.002919IJSEM 68: 2912-2917 201830016228
111218Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404341.1