Apilactobacillus timberlakei (DSM 104128, NRRL B-65472, HV_12)

Name: Apilactobacillus timberlakei (DSM 104128, NRRL B-65472, HV_12)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158412

Type strain

Strain Designation HV_12

Culture col. no. DSM 104128 NRRL B-65472 HV_12 JCM 33325

NCBI tax ID(s) 2008380

Special Collections DSMZ JCM Literature strains

History DSMZ <- J. Rothman, UC Riverside, Riverside, USA; HV_12 JCM <-- DSM 104128 <-- J. A. Rothman, Univ. of California, Riverside, USA; HV_12.

Links

Apilactobacillus timberlakei HV_12 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from gut of sweet bee Agapostemon.

Gram-positive rod-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400334

LMG 31487,DSM 104128

@ref 20215

Last LPSN update 2026-05-29 08:43:57

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Apilactobacillus

Species Apilactobacillus timberlakei

Full scientific name Apilactobacillus timberlakei (McFrederick et al. 2018) Zheng et al. 2020

Synonyms (1)

Lactobacillus timberlakei

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
65392	positive	0.8-1.6 µm	0.5-0.7 µm	rod-shaped
125438					92
125438	positive				92.979

Colony morphology

@ref	Colony color	Colony shape	Medium used
65392	white	circular	De Man, Rogosa, Sharpe agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65088	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
65088	positive	growth	30
65392	positive	growth	15-35
65392	positive	optimum	30-35
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH	PH range
65392	positive	optimum	5
65392	positive	growth	2-6	acidophile
65392		growth	7
65392		growth	>7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
65392	aerobe
125439	obligate aerobe	97.035

Spore formation

@ref	Spore formation	Confidence
65392
125439		98.981
125438		92.881

Observation

65392

Observationheterofermentive

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
65392	15824 ChEBI	D-fructose	+	builds acid from
65392	17634 ChEBI	D-glucose	+	builds acid from
65392	17234 ChEBI	glucose	+	fermentation

Antibiotic resistance

@ref	ChEBI	Metabolite	Resistance conc.	Sensitivity conc.
65392	28971	ampicillin	10 µg (disc)
65392	28669	bacitracin		10 Unit (disc)
65392	3393	carbenicillin		100 µg (disc)
65392	209807	cefoxitin		30 µg (disc)
65392	17698	chloramphenicol	30 µg (disc)
65392	48923	erythromycin		15 µg (disc)
65392	6104	kanamycin	30 µg (disc)
65392	17334	penicillin		10 Unit (disc)
65392	8309	polymyxin b		300 Unit (disc)
65392	17076	streptomycin		10 µg (disc)
65392	27902	tetracycline		30 µg (disc)
65392	28001	vancomycin	30 µg (disc)

Enzymes

@ref	Value	Activity	Ec
65392	acid phosphatase	+	3.1.3.2
65392	alkaline phosphatase	+	3.1.3.1
65392	catalase	+	1.11.1.6
65392	leucine arylamidase	+	3.4.11.1
65392	naphthol-AS-BI-phosphohydrolase	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure	Host species
65088	gut of sweet bee Agapostemon	33.9706N, 117.3201W	USA	USA	North America
65392	gut of a sweat bee (Agapostemon sp.)	campus of UC Riverside, Riverside, CA	USA	USA	North America	De Man, Rogosa, Sharpe agar	supplemented with 20 % D-fructose	2-3 days	24	Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C.
67770	Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental Research	Austin, TX	USA	USA	North America						Halictus ligatus

Taxonmaps

69479

Global distribution of 16S sequence KX656650 (>99% sequence identity) for Apilactobacillus micheneri subclade from Microbeatlas

Aquatic Samples	107
Soil Samples	84
Animal Samples	1575
Plant Samples	106
Total Samples	1872

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
67770	ASM299396v1 assembly for Apilactobacillus timberlakei HV_12	contig	GCA_002993965	2008380.3	2008380	73.15

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
65088	Apilactobacillus timberlakei strain HV_12 16S ribosomal RNA gene, partial sequence	KX656650	1431		2008380

GC content

@ref	GC-content (mol%)	Method
65088	30.5
65392	30.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM299396v1 assembly for Apilactobacillus timberlakei HV_12
NCBI: GCA_002993965

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.04	no
125439	gram_stain	BacteriaNetⓘ	negative	86.25	no
125439	motility	BacteriaNetⓘ	no	73.25	no
125439	spore_formation	BacteriaNetⓘ	no	98.98	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Apilactobacillus timberlakei HV_12
NCBI: GCA_002993965.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.98	no
125438	anaerobic	anaerobicⓘ	no	81.93	yes
125438	aerobic	aerobicⓘ	no	83.27	no
125438	spore-forming	spore-formingⓘ	no	92.88	no
125438	thermophilic	thermophileⓘ	no	94.50	yes
125438	flagellated	motile2+ⓘ	no	92.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers.	McFrederick QS, Vuong HQ, Rothman JA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002758	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65088	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104128
#65392	Quinn S. McFrederick, Hoang Q. Vuong, Jason A. Rothman: Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. IJSEM 68: 1879 - 1884 2018 ( DOI 10.1099/ijsem.0.002758 , PubMed 29648528 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Apilactobacillus timberlakei (DSM 104128, NRRL B-65472, HV_12)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Antibiotic resistance

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_002993965

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media