Name: Apilactobacillus timberlakei HV_12
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 158412

Type strain:

Species: Apilactobacillus timberlakei

Strain Designation: HV_12

Culture col. no.: DSM 104128, NRRL B-65472, JCM 33325

Strain history: DSM 104128 <-- J. A. Rothman, Univ. of California, Riverside, USA; HV_12.

NCBI tax ID(s): 2008380 (species)

SI-ID 400334

DSM 104128

Special Collections

Section

Apilactobacillus timberlakei HV_12 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from gut of sweet bee Agapostemon.

colony-forming
Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Apilactobacillus*
	Species	**Apilactobacillus timberlakei**
	Full Scientific Name (LPSN)	Apilactobacillus timberlakei (McFrederick et al. 2018) Zheng et al. 2020

Synonym

Lactobacillus timberlakei

Information on morphological and physiological properties Morphology

[Ref.: #65392]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %92.979

[Ref.: #65392]	Cell length	0.8-1.6 µm

[Ref.: #65392]	Cell width	0.5-0.7 µm

[Ref.: #65392]	Cell shape	rod-shaped

[Ref.: #65392]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %92

[Ref.: #65392]	Colony color	white
[Ref.: #65392]	Colony shape	circular
[Ref.: #65392]	Cultivation medium used	De Man, Rogosa, Sharpe agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65088]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #65088]

Culture medium growth

[Ref.: #65088]

Culture medium link

Medium recipe at MediaDive

[Ref.: #65088]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #65088]	growth	30 °C
[Ref.: #65392]	growth	15-35 °C
[Ref.: #65392]	optimum	30-35 °C
[Ref.: #67770]	growth	30 °C

	Kind of pH		pH
[Ref.: #65392]		optimum	5
[Ref.: #65392]		growth	2-6
[Ref.: #65392]	no	growth	7
[Ref.: #65392]	no	growth	>7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65392]

Oxygen tolerance

aerobe

[Ref.: #65392]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.881

[Ref.: #65392]

Observation

heterofermentive

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65392]	D-fructose ChEBI	+	builds acid from
[Ref.: #65392]	D-glucose ChEBI	+	builds acid from
[Ref.: #65392]	glucose ChEBI	+	fermentation

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65392]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #65392]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #65392]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #65392]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #65392]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #65392]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #65392]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #65392]	penicillin ChEBI	yes	10	Unit (disc)
[Ref.: #65392]	polymyxin b ChEBI	yes	300	Unit (disc)
[Ref.: #65392]	streptomycin ChEBI	yes	10	µg (disc)
[Ref.: #65392]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #65392]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #65392]	acid phosphatase	+	3.1.3.2
[Ref.: #65392]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65392]	catalase	+	1.11.1.6
[Ref.: #65392]	leucine arylamidase	+	3.4.11.1
[Ref.: #65392]	naphthol-AS-BI-phosphohydrolase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65088]	Sample type/isolated from	gut of sweet bee Agapostemon
[Ref.: #65088]	Geographic location (country and/or sea, region)	33.9706N, 117.3201W
[Ref.: #65088]	Country	USA
[Ref.: #65088]	Country ISO 3 Code	USA
[Ref.: #65088]	Continent	North America

[Ref.: #65392]	Sample type/isolated from	gut of a sweat bee (Agapostemon sp.)
[Ref.: #65392]	Geographic location (country and/or sea, region)	campus of UC Riverside, Riverside, CA
[Ref.: #65392]	Country	USA
[Ref.: #65392]	Country ISO 3 Code	USA
[Ref.: #65392]	Continent	North America
[Ref.: #65392]	Enrichment culture	De Man, Rogosa, Sharpe agar
[Ref.: #65392]	Enrichment culture composition	supplemented with 20 % D-fructose
[Ref.: #65392]	Enrichment culture duration	2-3 days
[Ref.: #65392]	Enrichment culture temperature	24 ̊C
[Ref.: #65392]	Isolation/enrichment procedure	Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C.

[Ref.: #67770]	Sample type/isolated from	Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental Research
[Ref.: #67770]	Host species	Halictus ligatus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Austin, TX
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX656650 (>99% sequence identity) for Apilactobacillus micheneri subclade from Microbeatlas

Aquatic Samples	107
Soil Samples	84
Animal Samples	1575
Plant Samples	106
Total Samples	1872

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65088]	Apilactobacillus timberlakei strain HV_12 16S ribosomal RNA gene, partial sequence	KX656650	1431	GenBank ENA	2008380 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Apilactobacillus timberlakei HV_12	GCA_002993965	contig	GenBank ENA	2008380 tax ID

[Ref.: #65088]	GC-content	30.5 mol%
[Ref.: #65392]	GC-content	30.5 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Apilactobacillus timberlakei HV_12 NCBI: GCA_002993965.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	92.979	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	81.932	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	83.269	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.881	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	92	yes

Availability in culture collections External links

[Ref.: #65088]

Culture collection no.

DSM 104128, NRRL B-65472, JCM 33325

[Ref.: #111215]

SI-ID 400334

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers.	McFrederick QS, Vuong HQ, Rothman JA	Int J Syst Evol Microbiol	10.1099/ijsem.0.002758	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65088

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104128

#65392

Quinn S. McFrederick, Hoang Q. Vuong, Jason A. Rothman: Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers. IJSEM 68: 1879 - 1884 2018 ( DOI 10.1099/ijsem.0.002758 , PubMed 29648528 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#111215

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400334.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Apilactobacillus timberlakei in external resources:
SILVA
BRENDA
PANGAEA
GBIF