Strain identifier

BacDive ID: 158413

Type strain: Yes

Species: Apilactobacillus micheneri

Strain Designation: Hlig3

Strain history: DSM 104126 <-- J. A. Rothman, Univ. of California, Riverside, USA; Hlig3.

NCBI tax ID(s): 1899430 (species)

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General

@ref: 65089

BacDive-ID: 158413

DSM-Number: 104126

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming

description: Apilactobacillus micheneri Hlig3 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from Adult bee gut, Halictus ligatus.

NCBI tax id

  • NCBI tax id: 1899430
  • Matching level: species

strain history

@refhistory
65089<- J. Rothman, UC Riverside, Riverside, USA; Hlig3<-,University of California, Riverside
67770DSM 104126 <-- J. A. Rothman, Univ. of California, Riverside, USA; Hlig3.

doi: 10.13145/bacdive158413.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Apilactobacillus
  • species: Apilactobacillus micheneri
  • full scientific name: Apilactobacillus micheneri (McFrederick et al. 2018) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Apilactobacillus kosoi
    20215Lactobacillus micheneri
    20215Lactobacillus kosoi

@ref: 65089

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Apilactobacillus

species: Apilactobacillus micheneri

full scientific name: Apilactobacillus micheneri (McFrederick et al. 2018) Zheng et al. 2020

strain designation: Hlig3

type strain: yes

Morphology

cell morphology

  • @ref: 65392
  • gram stain: positive
  • cell length: 0.9-1.6 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65392
  • colony color: white
  • colony shape: circular
  • medium used: De Man, Rogosa, Sharpe agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65089MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
65392De Man, Rogosa, Sharpe agaryeswith 20 % D-fructose agar

culture temp

@refgrowthtypetemperature
65089positivegrowth30
65392positivegrowth15-35
65392positiveoptimum30-35
67770positivegrowth30

culture pH

@refabilitytypepHPH range
65392positivegrowth2-6acidophile
65392positiveoptimum6
65392nogrowth7
65392nogrowth>7

Physiology and metabolism

oxygen tolerance

  • @ref: 65392
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65392
  • spore formation: no

observation

  • @ref: 65392
  • observation: heterofermentive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6539215824D-fructose+builds acid from
6539217634D-glucose+builds acid from
6539217234glucose+fermentation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6539217076streptomycinyesyes10 µg (disc)
6539228669bacitracinyesyes10 Unit (disc)
6539217334penicillinyesyes10 Unit (disc)
6539248923erythromycinyesyes15 µg (disc)
65392209807cefoxitinyesyes30 µg (disc)
6539227902tetracyclineyesyes30 µg (disc)
653923393carbenicillinyesyes100 µg (disc)
653928309polymyxin byesyes300 Unit (disc)
6539228971ampicillinyesyes10 µg (disc)
6539217698chloramphenicolyesyes30 µg (disc)
6539228001vancomycinyesyes30 µg (disc)
653926104kanamycinyesyes30 µg (disc)

enzymes

@refvalueactivityec
65392leucine arylamidase+3.4.11.1
65392acid phosphatase+3.1.3.2
65392naphthol-AS-BI-phosphohydrolase+
65392catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedurehost species
65089Adult bee gut, Halictus ligatus30.2357N, 97.6467WUSAUSANorth America
65392gut of a sweat bee (Halictus ligatus)Hornsby Bend Center for Environmental Research, Austin, TXUSAUSANorth AmericaDe Man, Rogosa, Sharpe agarsupplemented with 20 % D-fructose2-3 days24Bee guts were dissected in sterile water then homogenized in sterile physiologic saline. The gut homogenates were plated on De Man, Rogosa, Sharpe agar plates supplemented with 20 % D-fructose, then incubated in an aerobic environment for 2-3 days at 24°C.
67770Gut of a sweat bee (Halictus ligatus) caught at the Hornsby Bend Center for Environmental ResearchAustin, TXUSAUSANorth AmericaHalictus ligatus

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 65089
  • description: Apilactobacillus micheneri strain Hlig3 16S ribosomal RNA gene, partial sequence
  • accession: KT833121
  • length: 1549
  • database: nuccore
  • NCBI tax ID: 1899430

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Apilactobacillus micheneri Hlig32923762712draftimg1899430
67770Apilactobacillus micheneri Hlig3GCA_002993975contigncbi1899430

GC content

@refGC-contentmethod
6508930.7
6539230.7genome sequence analysis

External links

@ref: 65089

culture collection no.: DSM 104126, NRRL B-65473, JCM 33323

straininfo link

  • @ref: 111216
  • straininfo: 407777

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29648528Lactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers.McFrederick QS, Vuong HQ, Rothman JAInt J Syst Evol Microbiol10.1099/ijsem.0.0027582018Animals, Bacterial Typing Techniques, Base Composition, Bees/*microbiology, California, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flowers/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
35345581Exploring the genome of Lactobacillaceae spp. Sy-1 isolated from Heterotrigona itama honey.Syed Yaacob SN, Huyop F, Misson M, Abdul Wahab R, Huda NPeerJ10.7717/peerj.130532022

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65089Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104126Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104126)
65392Quinn S. McFrederick, Hoang Q. Vuong, Jason A. RothmanLactobacillus micheneri sp. nov., Lactobacillus timberlakei sp. nov. and Lactobacillus quenuiae sp. nov., lactic acid bacteria isolated from wild bees and flowers10.1099/ijsem.0.002758IJSEM 68: 1879-1884 201829648528
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
111216Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407777.1