Name: Lysinibacillus sphaericus SSII-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1528

Type strain:

Species: Lysinibacillus sphaericus

Strain Designation: SSII-1

Culture col. no.: DSM 1866

Strain history: <- A.A. Yousten <- S. Singer, SSII-1

NCBI tax ID(s): 1421 (species)

SI-ID 11869

LMG 17382, DSM 1866, ATCC 33721, KCTC 1529

Other strains from Lysinibacillus sphaericus

Section

Lysinibacillus sphaericus SSII-1 is a mesophilic human pathogen that produces toxins and was isolated from dead mosquito larva.

toxin production
human pathogen
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Caryophanaceae*
	Genus	*Lysinibacillus*
	Species	**Lysinibacillus sphaericus**
	Full Scientific Name (LPSN)	Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

	Synonym	Lysinibacillus varians
	Synonym	Bacillus sphaericus
	Synonym	Lysinibacillus mangiferahumi
	Synonym	Lysinibacillus mangiferihumi
	Synonym	Lysinibacillus tabacifolii

Information on culture and growth conditions Culture and growth conditions

[Ref.: #912]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #912]

Culture medium growth

[Ref.: #912]

Culture medium link

Medium recipe at MediaDive

[Ref.: #912]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #912]			growth	30 °C

[Ref.: #912]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #912]

Name of produced compound

mosquito toxin

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #912]	Sample type/isolated from	dead mosquito larva
[Ref.: #912]	Country	India
[Ref.: #912]	Country ISO 3 Code	IND
[Ref.: #912]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host	#Other	#Decomposing animal

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #912]	Pathogenicity (human)	yes, in single cases
[Ref.: #912]	Pathogenicity (animal)	yes
[Ref.: #912]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lysinibacillus sphaericus SSII-1	GCA_015335885	chromosome	GenBank	1421 tax ID	*
[Ref.: #66792]	Lysinibacillus sphaericus SSII-1	GCA_000733525	scaffold	GenBank	1421 tax ID	*
[Ref.: #66792]	Lysinibacillus sphaericus SSII-1	2609460276	draft	JGI IMG/M	1421 tax ID	*

[Ref.: #912]

GC-content

35.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lysinibacillus sphaericus SSII-1 NCBI: GCA_015335885.1
[Ref.: #69480]	motile	yes	89.606	no
[Ref.: #69480]	flagellated	yes	84.772	no
[Ref.: #69480]	gram-positive	yes	89.035	no
[Ref.: #69480]	anaerobic	no	97.742	no
[Ref.: #69480]	aerobic	yes	89.361	no
[Ref.: #69480]	halophile	no	93.12	no
[Ref.: #69480]	spore-forming	yes	96.733	no
[Ref.: #69480]	glucose-util	yes	86.314	no
[Ref.: #69480]	thermophile	no	99.926	yes
[Ref.: #69480]	glucose-ferment	no	88.768	no

Availability in culture collections External links

[Ref.: #912]

Culture collection no.

DSM 1866

[Ref.: #71174]

SI-ID 11869

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome comparison provides molecular insights into the phylogeny of the reassigned new genus Lysinibacillus.	Xu K, Yuan Z, Rayner S, Hu X	BMC Genomics	10.1186/s12864-015-1359-x	2015	*
Enzymology	From cosubstrate similarity to inhibitor diversity--inhibitors of ADP-ribosyltransferases from kinase inhibitor screening.	Maurer B, Mathias U, Papatheodorou P, Shekfeh S, Orth J, Jank T, Schwan C, Sippl W, Aktories K, Jung M	Mol Biosyst	10.1039/c0mb00151a	2010	*
Phylogeny	Interpreting the SDS-PAGE protein patterns with self-organizing maps: application for the characterization of mosquito-pathogenic Bacillus strains.	Sreshty MA, Misra S, Murty US	J Appl Microbiol	10.1111/j.1365-2672.2010.04880.x	2010	*
Enzymology	Cytolytic toxin Cyt1Aa of Bacillus thuringiensis synergizes the mosquitocidal toxin Mtx1 of Bacillus sphaericus.	Zhang B, Liu M, Yang Y, Yuan Z	Biosci Biotechnol Biochem	10.1271/bbb.60140	2006	*
Metabolism	Two-site autoinhibition of the ADP-ribosylating mosquitocidal toxin (MTX) from Bacillus sphaericus by its 70-kDa ricin-like binding domain.	Carpusca I, Schirmer J, Aktories K	Biochemistry	10.1021/bi048729y	2004	*
Metabolism	Toxicity of Bacillus sphaericus LP1-G against susceptible and resistant Culex quinquefasciatus and the cloning of the mosquitocidal toxin gene.	Shi YX, Zheng DS, Yuan ZM	Curr Microbiol	10.1007/s00284-002-3992-2	2003	*
Metabolism	Inactivation of the elongation factor Tu by mosquitocidal toxin-catalyzed mono-ADP-ribosylation.	Schirmer J, Wieden HJ, Rodnina MV, Aktories K	Appl Environ Microbiol	10.1128/AEM.68.10.4894-4899.2002	2002	*
Enzymology	The ADP-ribosylating mosquitocidal toxin from Bacillus sphaericus: proteolytic activation, enzyme activity, and cytotoxic effects.	Schirmer J, Just I, Aktories K	J Biol Chem	10.1074/jbc.M108463200	2002	*
Metabolism	Molecular cloning of an apoptosis-inducing protein, pierisin, from cabbage butterfly: possible involvement of ADP-ribosylation in its activity.	Watanabe M, Kono T, Matsushima-Hibiya Y, Kanazawa T, Nishisaka N, Kishimoto T, Koyama K, Sugimura T, Wakabayashi K	Proc Natl Acad Sci U S A	10.1073/pnas.96.19.10608	1999	*
Enzymology	Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilisins from Antarctic bacilli.	Wati MR, Thanabalu T, Porter AG	Biochim Biophys Acta	10.1016/s0167-4781(97)00023-7	1997	*
Pathogenicity	Unusual amino acid determinants of host range in the Mtx2 family of mosquitocidal toxins.	Chan SW, Thanabalu T, Wee BY, Porter AG	J Biol Chem	10.1074/jbc.271.24.14183	1996	*
Genetics	New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins.	Liu JW, Porter AG, Wee BY, Thanabalu T	Appl Environ Microbiol	10.1128/aem.62.6.2174-2176.1996	1996	*
Pathogenicity	A Bacillus sphaericus gene encoding a novel type of mosquitocidal toxin of 31.8 kDa.	Thanabalu T, Porter AG	Gene	10.1016/0378-1119(95)00836-5	1996	*
Pathogenicity	Biochemistry and mode of action of the Bacillus sphaericus toxins.	Davidson EW	Mem Inst Oswaldo Cruz	10.1590/s0074-02761995000100018	1995	*
Pathogenicity	Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.	Yap WH, Thanabalu T, Porter AG	Appl Environ Microbiol	10.1128/aem.60.11.4199-4202.1994	1994	*
Pathogenicity	Cytotoxicity and ADP-ribosylating activity of the mosquitocidal toxin from Bacillus sphaericus SSII-1: possible roles of the 27- and 70-kilodalton peptides.	Thanabalu T, Berry C, Hindley J	J Bacteriol	10.1128/jb.175.8.2314-2320.1993	1993	*
Pathogenicity	Proteolytic processing of the mosquitocidal toxin from Bacillus sphaericus SSII-1.	Thanabalu T, Hindley J, Berry C	J Bacteriol	10.1128/jb.174.15.5051-5056.1992	1992	*
Metabolism	Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae.	Thanabalu T, Hindley J, Brenner S, Oei C, Berry C	Appl Environ Microbiol	10.1128/aem.58.3.905-910.1992	1992	*
Enzymology	Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1.	Thanabalu T, Hindley J, Jackson-Yap J, Berry C	J Bacteriol	10.1128/jb.173.9.2776-2785.1991	1991	*
Pathogenicity	Comparative studies of the mosquito-larval toxin of Bacillus sphaericus SSII-1 and 1593.	Myers P, Yousten AA, Davidson EW	Can J Microbiol	10.1139/m79-193	1979	*
Pathogenicity	Toxic activity of Bacillus sphaericus SSII-1 for mosquito larvae.	Myers P, Yousten AA	Infect Immun	10.1128/iai.19.3.1047-1053.1978	1978	*
	Ultrastructure of midgut events in the pathogenesis of Bacillus sphaericus strain SSII-1 infections of Culex pipiens quinquefasciatus larvae.	Davidson EW	Can J Microbiol	10.1139/m79-028	1979	*

References

#912	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1866

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71174

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID11869.1 )

* These data were automatically processed and therefore are not curated

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