Lysinibacillus sphaericus (CIP 104788, ATCC 4525, CCM 2177)

Name: Lysinibacillus sphaericus (CIP 104788, ATCC 4525, CCM 2177)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 136346

Type strain

Strain Designation 25

Culture col. no. CIP 104788 ATCC 4525 CCM 2177 25

NCBI tax ID(s) 1421

Special Collections CIP

History CIP <- 1996, ATCC, Bacillus sphaericus <- W.W. Ford: strain 25

Links

Lysinibacillus sphaericus 25 is an obligate aerobe, spore-forming, Gram-positive bacterium of the family Caryophanaceae.

spore-forming Gram-positive motile rod-shaped obligate aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 46447

ATCC 4525,CCM 2177,CIP 104788,NCIMB 8218,IFO3526,NCTC2609,VKM B-713,NCIB 8218,NBRC 3526,NCAIM B.02068,LMG 23776

@ref 20215

Last LPSN update 2026-02-09 11:32:37

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Caryophanaceae

Genus Lysinibacillus

Species Lysinibacillus sphaericus

Full scientific name Lysinibacillus sphaericus (Meyer and Neide 1904) Ahmed et al. 2007

Synonyms (5)

Lysinibacillus tabacifolii
Lysinibacillus mangiferahumi
Lysinibacillus varians
Lysinibacillus mangiferihumi
Bacillus sphaericus

BacDive ID	Other strains from **Lysinibacillus sphaericus** (39)	Type strain
1532	L. sphaericus Gibson 1013, 1013, DSM 28, ATCC 14577, CCM ... (type strain)
1523	L. sphaericus S60-67, DSM 461
1524	L. sphaericus S64-67, DSM 462
1525	L. sphaericus E 1-65, DSM 463
1526	L. sphaericus 9602, DSM 396, NCTC 9602, CRBIP3.5052
1527	L. sphaericus Gibson 97, DSM 488, ATCC 19394, NCTC 4825
1528	L. sphaericus SSII-1, DSM 1866
1529	L. sphaericus 1593, DSM 1867
1530	L. sphaericus NRS-732, DSM 2899, ATCC 245, CRBIP3.2498, ...
1531	L. sphaericus Claussen, 1911, N.R. Smith, 966, Claussen, ...
1533	L. sphaericus DSM 7258, NRRL NRS-1198
1534	L. sphaericus DSM 7260, NRRL NRS-1691
1535	L. sphaericus DSM 7261, NRRL NRS-1692
1536	L. sphaericus DSM 8444, NRS 717, CRBIP3.5176
1543	L. sphaericus M-GX18, DSM 24076, CCTCC AB 2010389
130208	L. sphaericus K3514, K3514T, DSM 25877, CCTCC AB 2012050, ...
134592	L. sphaericus Army 87, CIP 106829, NRRL NRS-1186
135275	L. sphaericus CIP 103521, ATCC 17932
135935	L. sphaericus A20, CIP 106828, NRRL NRS-800
136490	L. sphaericus 209.87, CIP 102939
136560	L. sphaericus 14, CIP 106826, NRRL NRS-1695
137245	L. sphaericus CIP 103415
138013	L. sphaericus CIP 102465
138366	L. sphaericus CIP 106825, NRRL BD-99
138579	L. sphaericus 2518, CIP 51.25, CRBIP3.2497
139136	L. sphaericus CIP 51.16, CRBIP3.2496
139158	L. sphaericus 471, NRS-344, CIP 52.89, IAM 1286, IFO 3341, ...
141264	L. sphaericus CCUG 453 E
145746	L. sphaericus CCUG 29464, NRRL NRS-1756
146770	L. sphaericus CCUG 32090
147042	L. sphaericus CCUG 32712
147257	L. sphaericus CCUG 33213
150379	L. sphaericus CCUG 39777
151624	L. sphaericus CCUG 44478 B
152332	L. sphaericus CCUG 46460
152765	L. sphaericus CCUG 47570
153433	L. sphaericus CCUG 49579
156638	L. sphaericus CCUG 63782
162765	L. sphaericus JCM 20116, IAM 1228

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
35731	positive	rod-shaped

Colony morphology

35731

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
35731	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
35731	CIP Medium 3			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
35731	positive	growth	30
35731	positive	growth	22-45
35731	negative	growth	10
35731	negative	growth	55

Culture pH

@ref	Ability	Type	PH
35731	positive	growth	6

Physiology and metabolism

Oxygen tolerance

35731

Oxygen toleranceobligate aerobe

Spore formation

35731

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
35731	NaCl	positive	growth	0-6 %
35731	NaCl		growth	8 %
35731	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
35731	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
35731	4853 ChEBI	esculin	-	hydrolysis
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
35731	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
35731	17632 ChEBI	nitrate	-	reduction
35731	17632 ChEBI	nitrate	-	respiration
35731	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
35731	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
35731	15688 ChEBI	acetoin	-
35731	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
35731	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
35731	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
35731	caseinase	-	3.4.21.50
35731	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
35731	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
35731	gamma-glutamyltransferase	-	2.3.2.2
35731	gelatinase	-
35731	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
35731	lipase	-
68382	lipase (C 14)	-		from API zym
35731	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
35731	ornithine decarboxylase	-	4.1.1.17
35731	oxidase	+
35731	protease	-
68382	trypsin	-	3.4.21.4	from API zym
35731	tween esterase	-
35731	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
35731	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
35731	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
35731	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	+	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
35731	1	Risk group (French classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Lysinibacillus sphaericus strain ATCC 4525 16S ribosomal RNA gene, partial sequence.	MH173796	907		1421

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Optimization of a novel dental self-healing resin composite by bacteria-induced biomineralization.	Han Y, Zhang X, Weng J, Tian S, Dong X, Cai Z, Zhang Y, Wu T, Lin D, Zhu Y.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1590348	2025
	Land Use Influences the Composition and Antimicrobial Effects of Propolis.	Orth AJ, Curran EH, Haas EJ, Kraemer AC, Anderson AM, Mason NJ, Fassbinder-Orth CA.	Insects	10.3390/insects13030239	2022
Enzymology	Fast and easy protocol for the purification of recombinant S-layer protein for synthetic biology applications.	Norville JE, Kelly DF, Knight TF, Belcher AM, Walz T.	Biotechnol J	10.1002/biot.201100024	2011
Proteome	Direct observation of kinetic traps associated with structural transformations leading to multiple pathways of S-layer assembly.	Shin SH, Chung S, Sanii B, Comolli LR, Bertozzi CR, De Yoreo JJ.	Proc Natl Acad Sci U S A	10.1073/pnas.1201504109	2012
	Self-catalyzed growth of S layers via an amorphous-to-crystalline transition limited by folding kinetics.	Chung S, Shin SH, Bertozzi CR, De Yoreo JJ.	Proc Natl Acad Sci U S A	10.1073/pnas.1008280107	2010
Metabolism	Nucleoid Size Scaling and Intracellular Organization of Translation across Bacteria.	Gray WT, Govers SK, Xiang Y, Parry BR, Campos M, Kim S, Jacobs-Wagner C.	Cell	10.1016/j.cell.2019.05.017	2019
	Enzymatic Conversion of CO₂: From Natural to Artificial Utilization.	Bierbaumer S, Nattermann M, Schulz L, Zschoche R, Erb TJ, Winkler CK, Tinzl M, Glueck SM.	Chem Rev	10.1021/acs.chemrev.2c00581	2023
	Formation of planar hybrid lipid/polymer membranes anchored to an S-layer protein lattice by vesicle binding and rupture.	Czernohlavek C, Schuster B.	Soft Mater	10.1080/1539445x.2019.1708753	2020
Enzymology	Electrochemical Biosensors Based on S-Layer Proteins.	Damiati S, Schuster B.	Sensors (Basel)	10.3390/s20061721	2020
	Formation and characteristics of mixed lipid/polymer membranes on a crystalline surface-layer protein lattice.	Czernohlavek C, Schuster B.	Biointerphases	10.1116/1.5132390	2020
Metabolism	S-layer fusion proteins--construction principles and applications.	Ilk N, Egelseer EM, Sleytr UB.	Curr Opin Biotechnol	10.1016/j.copbio.2011.05.510	2011
Pathogenicity	Effect of Spheroidal Age on Sorafenib Diffusivity and Toxicity in a 3D HepG2 Spheroid Model.	Eilenberger C, Rothbauer M, Ehmoser EK, Ertl P, Kupcu S.	Sci Rep	10.1038/s41598-019-41273-3	2019
Enzymology	Impact of surface wettability on S-layer recrystallization: a real-time characterization by QCM-D.	Iturri J, Vianna AC, Moreno-Cencerrado A, Pum D, Sleytr UB, Toca-Herrera JL.	Beilstein J Nanotechnol	10.3762/bjnano.8.10	2017
Metabolism	Probing peptide and protein insertion in a biomimetic S-layer supported lipid membrane platform.	Damiati S, Schrems A, Sinner EK, Sleytr UB, Schuster B.	Int J Mol Sci	10.3390/ijms16022824	2015
Metabolism	S-Layer Protein-Based Biosensors.	Schuster B.	Biosensors (Basel)	10.3390/bios8020040	2018
Metabolism	Pyruvate Substitutions on Glycoconjugates.	Hager FF, Sutzl L, Stefanovic C, Blaukopf M, Schaffer C.	Int J Mol Sci	10.3390/ijms20194929	2019
Pathogenicity	Bacterial components as naturally inspired nano-carriers for drug/gene delivery and immunization: Set the bugs to work?	Farjadian F, Moghoofei M, Mirkiani S, Ghasemi A, Rabiee N, Hadifar S, Beyzavi A, Karimi M, Hamblin MR.	Biotechnol Adv	10.1016/j.biotechadv.2018.02.016	2018
	Composite S-layer lipid structures.	Schuster B, Sleytr UB.	J Struct Biol	10.1016/j.jsb.2009.03.004	2009
	Cell surface display of chimeric glycoproteins via the S-layer of Paenibacillus alvei.	Zarschler K, Janesch B, Kainz B, Ristl R, Messner P, Schaffer C.	Carbohydr Res	10.1016/j.carres.2010.04.010	2010
Metabolism	S-layers: principles and applications.	Sleytr UB, Schuster B, Egelseer EM, Pum D.	FEMS Microbiol Rev	10.1111/1574-6976.12063	2014
	Relevance of glycosylation of S-layer proteins for cell surface properties.	Schuster B, Sleytr UB.	Acta Biomater	10.1016/j.actbio.2015.03.020	2015
Metabolism	Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury.	Francois F, Lombard C, Guigner JM, Soreau P, Brian-Jaisson F, Martino G, Vandervennet M, Garcia D, Molinier AL, Pignol D, Peduzzi J, Zirah S, Rebuffat S.	Appl Environ Microbiol	10.1128/aem.06522-11	2012
Metabolism	Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41.	Li J, Hu X, Yan J, Yuan Z.	Appl Environ Microbiol	10.1128/aem.00356-09	2009
	Solid supported lipid membranes: new concepts for the biomimetic functionalization of solid surfaces.	Knoll W, Naumann R, Friedrich M, Robertson JW, Losche M, Heinrich F, McGillivray DJ, Schuster B, Gufler PC, Pum D, Sleytr UB.	Biointerphases	10.1116/1.2913612	2008
	S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting.	Ucisik MH, Kupcu S, Breitwieser A, Gelbmann N, Schuster B, Sleytr UB.	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2015.01.055	2015
	Emulsomes meet S-layer proteins: an emerging targeted drug delivery system.	Ucisik MH, Sleytr UB, Schuster B.	Curr Pharm Biotechnol	10.2174/138920101604150218112656	2015
Metabolism	Lactobacillus surface layer proteins: structure, function and applications.	Hynonen U, Palva A.	Appl Microbiol Biotechnol	10.1007/s00253-013-4962-2	2013
Biotechnology	Biomimetic interfaces based on S-layer proteins, lipid membranes and functional biomolecules.	Schuster B, Sleytr UB.	J R Soc Interface	10.1098/rsif.2014.0232	2014
Metabolism	Emerging facets of prokaryotic glycosylation.	Schaffer C, Messner P.	FEMS Microbiol Rev	10.1093/femsre/fuw036	2017
	Bacterial cell-envelope glycoconjugates.	Messner P, Schaffer C, Kosma P.	Adv Carbohydr Chem Biochem	10.1016/b978-0-12-408093-5.00006-x	2013
	A New Method for Dispersing Pristine Carbon Nanotubes Using Regularly Arranged S-Layer Proteins.	Breitwieser A, Sleytr UB, Pum D	Nanomaterials (Basel)	10.3390/nano11051346	2021
Pathogenicity	Cation-chelation and pH induced controlled switching of the non-fouling properties of bacterial crystalline films.	Iturri J, Moreno-Cencerrado A, Toca-Herrera JL	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2017.07.003	2017
Metabolism	In Vitro Characterization of the Two-Stage Non-Classical Reassembly Pathway of S-Layers.	Breitwieser A, Iturri J, Toca-Herrera JL, Sleytr UB, Pum D	Int J Mol Sci	10.3390/ijms18020400	2017
Metabolism	Surface-layer lattices as patterning element for multimeric extremozymes.	Ferner-Ortner-Bleckmann J, Gelbmann N, Tesarz M, Egelseer EM, Sleytr UB	Small	10.1002/smll.201201014	2013
Pathogenicity	Nanobiotechnology advanced antifouling surfaces for the continuous electrochemical monitoring of glucose in whole blood using a lab-on-a-chip.	Picher MM, Kupcu S, Huang CJ, Dostalek J, Pum D, Sleytr UB, Ertl P	Lab Chip	10.1039/c3lc41308j	2013
Metabolism	Identification of a novel gene cluster in the upstream region of the S-layer gene sbpA involved in cell wall metabolism of Lysinibacillus sphaericus CCM 2177 and characterization of the recombinantly produced autolysin and pyruvyl transferase.	Pleschberger M, Hildner F, Runzler D, Gelbmann N, Mayer HF, Sleytr UB, Egelseer EM	Arch Microbiol	10.1007/s00203-013-0876-8	2013
Enzymology	Multitechnique study on a recombinantly produced Bacillus halodurans laccase and an S-layer/laccase fusion protein.	Ferner-Ortner-Bleckmann J, Schrems A, Ilk N, Egelseer EM, Sleytr UB, Schuster B	Biointerphases	10.1116/1.3589284	2011
	Structural control of surface layer proteins at electrified interfaces investigated by in situ Fourier transform infrared spectroscopy.	Zafiu C, Trettenhahn G, Pum D, Sleytr UB, Kautek W	Phys Chem Chem Phys	10.1039/c0cp02127j	2011
Metabolism	Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy.	Ilk N, Schumi CT, Bohle B, Egelseer EM, Sleytr UB	Microb Cell Fact	10.1186/1475-2859-10-6	2011
Metabolism	Electrochemical control of adsorption dynamics of surface layer proteins on gold.	Zafiu C, Trettenhahn G, Pum D, Sleytr UB, Kautek W	Phys Chem Chem Phys	10.1039/c0cp01099e	2010
Metabolism	Self-assembly and recrystallization of bacterial S-layer proteins of Bacillus sphaericus and Bacillus thuringiensis on silicone, mica and quartz crystal supports.	Habibi N, Caneva Soumetz F, Giulianelli M, Pastorino L, Herrera O, Sbrana F, Raiteri R, Ruggiero C	Annu Int Conf IEEE Eng Med Biol Soc	10.1109/IEMBS.2010.5627499	2010
Metabolism	S-layer templated bioinspired synthesis of silica.	Gobel C, Schuster B, Baurecht D, Sleytr UB, Pum D	Colloids Surf B Biointerfaces	10.1016/j.colsurfb.2009.09.037	2009
Metabolism	Genetic engineering of the S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays.	Badelt-Lichtblau H, Kainz B, Vollenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N	Bioconjug Chem	10.1021/bc800445r	2009
	Detection of metal binding sites on functional S-layer nanoarrays using single molecule force spectroscopy.	Tang J, Ebner A, Kraxberger B, Leitner M, Hykollari A, Kepplinger C, Grunwald C, Gruber HJ, Tampe R, Sleytr UB, Ilk N, Hinterdorfer P	J Struct Biol	10.1016/j.jsb.2009.02.003	2009
Metabolism	Self-assembly of S-layer-enveloped cytochrome c polyelectrolyte multilayers.	Dronov R, Kurth DG, Mohwald H, Scheller FW, Friedmann J, Pum D, Sleytr UB, Lisdat F	Langmuir	10.1021/la8006958	2008
	Thermal stability, mechanical properties and content of bacterial protein layers recrystallized on polyelectrolyte multilayers.	Delcea M, Krastev R, Gutberlet T, Pum D, Sleytr UB, Toca-Herrera JL	Soft Matter	10.1039/b719408k	2008
Metabolism	Display of a peptide mimotope on a crystalline bacterial cell surface layer (S-layer) lattice for diagnosis of Epstein-Barr virus infection.	Tschiggerl H, Casey JL, Parisi K, Foley M, Sleytr UB	Bioconjug Chem	10.1021/bc7003523	2008
	Mapping bacterial surface layers affinity to polyelectrolytes through the building of hybrid macromolecular structures.	Delcea M, Krastev R, Gutlebert T, Pum D, Sleytr UB, Toca-Herrera JL	J Nanosci Nanotechnol	10.1166/jnn.2007.912	2007
Enzymology	Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus.	Tschiggerl H, Breitwieser A, de Roo G, Verwoerd T, Schaffer C, Sleytr UB	J Biotechnol	10.1016/j.jbiotec.2007.09.018	2007
	High-affinity tags fused to s-layer proteins probed by atomic force microscopy.	Tang J, Ebner A, Ilk N, Lichtblau H, Huber C, Zhu R, Pum D, Leitner M, Pastushenko V, Gruber HJ, Sleytr UB, Hinterdorfer P	Langmuir	10.1021/la702276k	2007
Transcriptome	Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development.	Huber C, Liu J, Egelseer EM, Moll D, Knoll W, Sleytr UB, Sara M	Small	10.1002/smll.200500147	2006
Metabolism	Recrystallization of bacterial S-layers on flat polyelectrolyte surfaces and hollow polyelectrolyte capsules.	Toca-Herrera JL, Krastev R, Bosio V, Kupcu S, Pum D, Fery A, Sara M, Sleytr UB	Small	10.1002/smll.200400035	2005
Metabolism	The three S-layer-like homology motifs of the S-layer protein SbpA of Bacillus sphaericus CCM 2177 are not sufficient for binding to the pyruvylated secondary cell wall polymer.	Huber C, Ilk N, Runzler D, Egelseer EM, Weigert S, Sleytr UB, Sara M	Mol Microbiol	10.1111/j.1365-2958.2004.04351.x	2005
Enzymology	An S-layer heavy chain camel antibody fusion protein for generation of a nanopatterned sensing layer to detect the prostate-specific antigen by surface plasmon resonance technology.	Pleschberger M, Saerens D, Weigert S, Sleytr UB, Muyldermans S, Sara M, Egelseer EM	Bioconjug Chem	10.1021/bc049964w	2004
Enzymology	Construction of a functional S-layer fusion protein comprising an immunoglobulin G-binding domain for development of specific adsorbents for extracorporeal blood purification.	Vollenkle C, Weigert S, Ilk N, Egelseer E, Weber V, Loth F, Falkenhagen D, Sleytr UB, Sara M	Appl Environ Microbiol	10.1128/AEM.70.3.1514-1521.2004	2004
	Highly robust lipid membranes on crystalline S-layer supports investigated by electrochemical impedance spectroscopy.	Gufler PC, Pum D, Sleytr UB, Schuster B	Biochim Biophys Acta	10.1016/j.bbamem.2003.12.009	2004
Metabolism	A functional chimaeric S-layer-enhanced green fluorescent protein to follow the uptake of S-layer-coated liposomes into eukaryotic cells.	Ilk N, Kupcu S, Moncayo G, Klimt S, Ecker RC, Hofer-Warbinek R, Egelseer EM, Sleytr UB, Sara M	Biochem J	10.1042/BJ20031900	2004
Metabolism	Self-assembly and recrystallization of bacterial S-layer proteins at silicon supports imaged in real time by atomic force microscopy.	Gyorvary ES, Stein O, Pum D, Sleytr UB	J Microsc	10.1111/j.1365-2818.2003.01270.x	2003
Enzymology	Generation of a functional monomolecular protein lattice consisting of an s-layer fusion protein comprising the variable domain of a camel heavy chain antibody.	Pleschberger M, Neubauer A, Egelseer EM, Weigert S, Lindner B, Sleytr UB, Muyldermans S, Sara M	Bioconjug Chem	10.1021/bc025603+	2003
Enzymology	Molecular characterization of the S-layer gene, sbpA, of Bacillus sphaericus CCM 2177 and production of a functional S-layer fusion protein with the ability to recrystallize in a defined orientation while presenting the fused allergen.	Ilk N, Vollenkle C, Egelseer EM, Breitwieser A, Sleytr UB, Sara M	Appl Environ Microbiol	10.1128/AEM.68.7.3251-3260.2002	2002
Metabolism	Structural and functional analyses of the secondary cell wall polymer of Bacillus sphaericus CCM 2177 that serves as an S-layer-specific anchor.	Ilk N, Kosma P, Puchberger M, Egelseer EM, Mayer HF, Sleytr UB, Sara M	J Bacteriol	10.1128/JB.181.24.7643-7646.1999	1999
Lipids	Bacterial S-layer protein coupling to lipids: x-ray reflectivity and grazing incidence diffraction studies.	Weygand M, Wetzer B, Pum D, Sleytr UB, Cuvillier N, Kjaer K, Howes PB, Losche M	Biophys J	10.1016/S0006-3495(99)77213-9	1999
	S-layer stabilized solid support lipid bilayers.	Wetzer B, Pum D, Sleytr UB	J Struct Biol	10.1006/jsbi.1997.3867	1997
Pathogenicity	Immunoreactivity of allergen (Bet v 1) conjugated to crystalline bacterial cell surface layers (S-layers).	Jahn-Schmid B, Graninger M, Glozik M, Kupcu S, Ebner C, Unger FM, Sleytr UB, Messner P	Immunotechnology	10.1016/1380-2933(96)00041-3	1996
	Scanning force microscopy studies of the S-layers from Bacillus coagulans E38-66, Bacillus sphaericus CCM2177 and of an antibody binding process.	Ohnesorge F, Heckl WM, Haberle W, Pum D, Sara M, Schindler H, Schilcher K, Kiener A, Smith DP, Sleytr UB, et al.	Ultramicroscopy	10.1016/0304-3991(92)90429-n	1992
Enzymology	Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525.	Li H, Zhu D, Hyatt BA, Malik FM, Biehl ER, Hua L	Appl Biochem Biotechnol	10.1007/s12010-008-8304-2	2008
Metabolism	Novel surface layer protein genes in Bacillus sphaericus associated with unusual insertion elements.	Pollmann K, Raff J, Schnorpfeil M, Radeva G, Selenska-Pobell S	Microbiology (Reading)	10.1099/mic.0.28201-0	2005

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#35731	Collection of Institut Pasteur ; Curators of the CIP; CIP 104788
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lysinibacillus sphaericus (CIP 104788, ATCC 4525, CCM 2177)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API biotype100

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

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