Name: Sphingobacterium mizutaii NCTC 12149
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14126

Type strain:

Species: Sphingobacterium mizutaii

Culture col. no.: NCTC 12149, DSM 11724, ATCC 33299, CCUG 15907, GIFU 1203, IFO 14946, LMG 8340, NBRC 14946, JCM 21155, CIP 101122, IAM 14315

Strain history: IAM 14315 <-- IFO 14946 <-- GIFU 1203 <-- S. Mizuta <-- N. Satoh strain Teraoka.

NCBI tax ID(s): 1220576 (strain), 1010 (species)

Other strains from Sphingobacterium mizutaii

S. mizutaii CCUG 15908
S. mizutaii CCUG 26768

Section

Sphingobacterium mizutaii NCTC 12149 is a mesophilic human pathogen that was isolated from ventricular fluid.

human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Sphingobacteriia*
	Order	*Sphingobacteriales*
	Family	*Sphingobacteriaceae*
	Genus	*Sphingobacterium*
	Species	**Sphingobacterium mizutaii**
	Full Scientific Name (PNU)	Sphingobacterium mizutaii corrig. Yabuuchi et al. 1983

	Synonym	Sphingobacterium mizutae
	Synonym	Flavobacterium mizutaii

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4456]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4456]

Culture medium growth

[Ref.: #4456]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4456]

Culture medium composition

expand / minimize

[Ref.: #39762]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #39762]

Culture medium growth

[Ref.: #39762]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #4456]	growth	28 °C
[Ref.: #39762]	growth	25 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #4456]	Temperature range	mesophilic
[Ref.: #39762]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68369]		indole ChEBI	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #46376]	7282	-	-	-	-	-	+	-	+	+	-	+	-	+	+	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #46376]	3643	-	+	-	+	-	+	-	-	-	-	+	-	-	-	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4456]	Sample type/isolated from	ventricular fluid

[Ref.: #46376]	Sample type/isolated from	Human ventricular fluid,fetus

[Ref.: #67770]	Sample type/isolated from	Ventricular fluid of fetus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Nagasaki
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Organ	#Brain
#Host Body Product	#Fluids	#Cerebrospinal fluid

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JF708889 (>99% sequence identity) for Sphingobacterium mizutaii subclade from Microbeatlas

Aquatic Samples	192
Soil Samples	181
Animal Samples	333
Plant Samples	46
Total Samples	752

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4456]	Pathogenicity (human)	yes, in single cases
[Ref.: #4456]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Sphingobacterium mizutaii gene for 16S rRNA, partial sequence, strain: NBRC 14946	AB680716	1453	ENA	1010 tax ID	*
[Ref.: #20218]	Sphingobacterium mizutae 16S ribosomal RNA	D14024	1270	ENA	1010 tax ID	*
[Ref.: #20218]	Sphingobacterium mizutaii strain LMG 8340 16S ribosomal RNA gene, partial sequence	JF708889	1454	ENA	1010 tax ID	*
[Ref.: #20218]	Flavobacterium mizutaii 16S ribosomal RNA gene, partial sequence	M58796	1478	ENA	1010 tax ID	*
[Ref.: #20218]	S.mizutae (ATCC 33299T) gene for 16S rRNA	X67853	795	ENA	1010 tax ID	*
[Ref.: #4456]	Flavobacterium mizutaii partial 16S rRNA, strain DSM 11724T	AJ438175	1491	ENA	1010 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Sphingobacterium mizutaii NCTC12149	GCA_900187125	complete	GenBank	1010 tax ID	*
[Ref.: #66792]	Sphingobacterium mizutaii NBRC 14946 = DSM 11724	1220576.3	wgs	PATRIC	1220576 tax ID	*
[Ref.: #66792]	Sphingobacterium mizutaii strain DSM 11724	1010.7	wgs	PATRIC	1010 tax ID	*
[Ref.: #66792]	Sphingobacterium mizutaii strain NCTC12149	1010.5	complete	PATRIC	1010 tax ID	*
[Ref.: #66792]	Sphingobacterium mizutaii NCTC 12149	2773857900	complete	JGI IMG/M	1010 tax ID	*
[Ref.: #66792]	Sphingobacterium mizutaii DSM 11724	2634166301	draft	JGI IMG/M	1220576 tax ID	*

[Ref.: #67770]

GC-content

40 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #4456]

Culture collection no.

NCTC 12149, DSM 11724, ATCC 33299, CCUG 15907, GIFU 1203, IFO 14946, LMG 8340, NBRC 14946, JCM 21155, CIP 101122, IAM 14315

[Ref.: #20218]

Associated Passport(s) in StrainInfo

278362, 11540, 11541, 11544, 11545, 11539, 487283

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Structural analysis of sphingophospholipids derived from Sphingobacterium spiritivorum, the type species of genus Sphingobacterium.	Naka T, Fujiwara N, Yano I, Maeda S, Doe M, Minamino M, Ikeda N, Kato Y, Watabe K, Kumazawa Y, Tomiyasu I, Kobayashi K	Biochim Biophys Acta	10.1016/j.bbalip.2003.10.010	2003	*
Phylogeny	Sphingobacterium daejeonense sp. nov., isolated from a compost sample.	Kim KH, Ten LN, Liu QM, Im WT, Lee ST	Int J Syst Evol Microbiol	10.1099/ijs.0.64406-0	2006	*
Phylogeny	Sphingobacterium composti sp. nov., isolated from cotton-waste composts.	Yoo SH, Weon HY, Jang HB, Kim BY, Kwon SW, Go SJ, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.64948-0	2007	*
Phylogeny	Sphingobacterium anhuiense sp. nov., isolated from forest soil.	Wei W, Zhou Y, Wang X, Huang X, Lai R	Int J Syst Evol Microbiol	10.1099/ijs.0.65864-0	2008	*
Phylogeny	Sphingobacterium shayense sp. nov., isolated from forest soil.	He X, Xiao T, Kuang H, Lan X, Tudahong M, Osman G, Fang C, Rahman E	Int J Syst Evol Microbiol	10.1099/ijs.0.018481-0	2009	*
Phylogeny	Sphingobacterium wenxiniae sp. nov., a cypermethrin-degrading species from activated sludge.	Zhang J, Zheng JW, Cho BC, Hwang CY, Fang C, He J, Li SP	Int J Syst Evol Microbiol	10.1099/ijs.0.033118-0	2011	*
Phylogeny	Sphingobacterium kyonggiense sp. nov., isolated from chloroethene-contaminated soil, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium mizutaii.	Choi HA, Lee SS	Int J Syst Evol Microbiol	10.1099/ijs.0.024737-0	2011	*
Phylogeny	Isolates belonging to CDC group II-i belong predominantly to Sphingobacterium mizutaii Yabuuchi et al. 1983: emended descriptions of S. mizutaii and of the genus Sphingobacterium.	Wauters G, Janssens M, De Baere T, Vaneechoutte M, Deschaght P	Int J Syst Evol Microbiol	10.1099/ijs.0.037325-0	2011	*
Phylogeny	Sphingobacterium hotanense sp. nov., isolated from soil of a Populus euphratica forest, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense.	Xiao T, He X, Cheng G, Kuang H, Ma X, Yusup K, Hamdun M, Gulsimay A, Fang C, Rahman E	Int J Syst Evol Microbiol	10.1099/ijs.0.030155-0	2012	*
Phylogeny	Sphingobacteriumsoli sp. nov., isolated from soil.	Fu YS, Hussain F, Habib N, Khan IU, Chu X, Duan YQ, Zhi XY, Chen X, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001946	2017	*
Phylogeny	Sphingobacterium olei sp. nov., isolated from oil-contaminated soil.	Liu B, Yang X, Sheng M, Yang Z, Qiu J, Wang C, He J	Int J Syst Evol Microbiol	10.1099/ijsem.0.004000	2020	*

References

#4456

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11724

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39762 ; Curators of the CIP;
#46376 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 15907

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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