Name: Megasphaera hexanoica MH
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140555

Type strain:

Species: Megasphaera hexanoica

Strain Designation: MH

Culture col. no.: KCCM 43214, JCM 31403, KFCC 11466P

Strain history: B.-I. Sang; Hanyang Univ., South Korea; KFCC 11466P.

NCBI tax ID(s): 1675036 (species)

Other strains from Megasphaera hexanoica

M. hexanoica Oil+RF-744-FAT-6-1, DSM 106893

Section

Megasphaera hexanoica MH is an anaerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from cow rumen.

coccus-shaped
colony-forming
Gram-negative
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Negativicutes*
	Order	*Veillonellales*
	Family	*Veillonellaceae*
	Genus	*Megasphaera*
	Species	**Megasphaera hexanoica**
	Full Scientific Name (LPSN)	Megasphaera hexanoica Jeon et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43304]	Gram stain	negative

[Ref.: #43304]	Cell length	0.8-1.2 µm

[Ref.: #43304]	Cell width	0.5-0.8 µm

[Ref.: #43304]	Cell shape	coccus-shaped

[Ref.: #43304]	Motility	no

[Ref.: #43304]	Colony size	0.5-1 mm
[Ref.: #43304]	Colony color	slightly yellowish, convex and opaque
[Ref.: #43304]	Colony shape	circular
[Ref.: #43304]	Incubation period	6-7 days
[Ref.: #43304]	Cultivation medium used	mPYF

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43304]

Culture medium

mPYF

[Ref.: #43304]

Culture medium growth

		Temperature
[Ref.: #43304]	growth	30-40 °C
[Ref.: #43304]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #43304]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43304]			optimum	6.5
[Ref.: #43304]			growth	5.5-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43304]

Oxygen tolerance

anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43304]	acetate ChEBI	+	growth
[Ref.: #43304]	amygdalin ChEBI	-	builds acid from
[Ref.: #43304]	arbutin ChEBI	-	builds acid from
[Ref.: #43304]	arginine ChEBI	-	hydrolysis
[Ref.: #43304]	butyrate ChEBI	+	growth
[Ref.: #43304]	cellobiose ChEBI	-	builds acid from
[Ref.: #43304]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43304]	D-arabitol ChEBI	+	builds acid from
[Ref.: #43304]	D-fructose ChEBI	+	builds acid from
[Ref.: #43304]	D-fucose ChEBI	-	builds acid from
[Ref.: #43304]	D-galactose ChEBI	+	assimilation
[Ref.: #43304]	D-glucose ChEBI	-	builds acid from
[Ref.: #43304]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43304]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43304]	D-mannose ChEBI	+	carbon source
[Ref.: #43304]	D-ribose ChEBI	-	builds acid from
[Ref.: #43304]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43304]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43304]	D-xylose ChEBI	-	builds acid from
[Ref.: #43304]	erythritol ChEBI	-	builds acid from
[Ref.: #43304]	esculin ChEBI	-	hydrolysis
[Ref.: #43304]	esculin ferric citrate	-	builds acid from
[Ref.: #43304]	galactitol ChEBI	-	builds acid from
[Ref.: #43304]	galactose ChEBI	+/-	carbon source
[Ref.: #43304]	gelatin ChEBI	-	hydrolysis
[Ref.: #43304]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43304]	gluconate ChEBI	+	assimilation
[Ref.: #43304]	glycerol ChEBI	-	builds acid from
[Ref.: #43304]	glycogen ChEBI	-	builds acid from
[Ref.: #43304]	inulin ChEBI	-	builds acid from
[Ref.: #43304]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43304]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43304]	L-fucose ChEBI	-	builds acid from
[Ref.: #43304]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43304]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43304]	lactate ChEBI	+/-	carbon source
[Ref.: #43304]	lactose ChEBI	-	builds acid from
[Ref.: #43304]	maltose	-	builds acid from
[Ref.: #43304]	mannitol ChEBI	-	assimilation
[Ref.: #43304]	melezitose ChEBI	-	builds acid from
[Ref.: #43304]	melibiose ChEBI	-	builds acid from
[Ref.: #43304]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #43304]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43304]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #43304]	myo-inositol ChEBI	+	builds acid from
[Ref.: #43304]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43304]	nitrate ChEBI	-	reduction
[Ref.: #43304]	potassium 2-dehydro-D-gluconate	-	builds acid from
[Ref.: #43304]	potassium 5-dehydro-D-gluconate	+	builds acid from
[Ref.: #43304]	potassium gluconate ChEBI	+	builds acid from
[Ref.: #43304]	pyruvate ChEBI	+	assimilation
[Ref.: #43304]	raffinose ChEBI	-	builds acid from
[Ref.: #43304]	ribitol ChEBI	-	builds acid from
[Ref.: #43304]	salicin ChEBI	-	builds acid from
[Ref.: #43304]	starch ChEBI	-	builds acid from
[Ref.: #43304]	sucrose ChEBI	-	builds acid from
[Ref.: #43304]	trehalose ChEBI	-	builds acid from
[Ref.: #43304]	turanose ChEBI	-	builds acid from
[Ref.: #43304]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43304]		colistin ChEBI	yes	10	µg (disc)
[Ref.: #43304]		vancomycin ChEBI				yes	5	µg (disc)

	Metabolite	Production
[Ref.: #43304]	acetoin ChEBI	no
[Ref.: #43304]	carbon dioxide ChEBI	yes
[Ref.: #43304]	dihydrogen ChEBI	yes
[Ref.: #43304]	hydrogen sulfide ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43304]		catalase	-	1.11.1.6
[Ref.: #43304]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43304]	Sample type/isolated from	cow rumen

[Ref.: #67770]	Sample type/isolated from	Cow rumen
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Rumen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KX021300 (>99% sequence identity) for Veillonellaceae from Microbeatlas

Aquatic Samples	2091
Soil Samples	625
Animal Samples	42073
Plant Samples	397
Total Samples	45186

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43304]	16S rRNA	KX021300		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Megasphaera hexanoica MH	GCA_003315775	complete	GenBank	1675036 tax ID	*
[Ref.: #66792]	Megasphaera hexanoica MH	2841216226	complete	JGI IMG/M	1675036 tax ID	*

[Ref.: #43304]

GC-content

51.8 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Megasphaera hexanoica MH NCBI: GCA_003315775.1
[Ref.: #69480]	motile	no	90.982	yes
[Ref.: #69480]	flagellated	no	95.724	no
[Ref.: #69480]	gram-positive	no	95.979	yes
[Ref.: #69480]	anaerobic	yes	99.475	yes
[Ref.: #69480]	aerobic	no	97.975	yes
[Ref.: #69480]	halophile	no	75.168	no
[Ref.: #69480]	spore-forming	no	90.457	no
[Ref.: #69480]	glucose-util	yes	69.122	no
[Ref.: #69480]	thermophile	no	98.65	yes
[Ref.: #69480]	glucose-ferment	yes	55.845	no

Availability in culture collections External links

[Ref.: #43304]

Culture collection no.

KCCM 43214, JCM 31403, KFCC 11466P

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.	Jeon BS, Kim S, Sang BI	Int J Syst Evol Microbiol	10.1099/ijsem.0.001888	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43304

Byoung Seung Jeon, Seil Kim, Byoung-In Sang: Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen. IJSEM 67: 2114 - 2120 2017 ( DOI 10.1099/ijsem.0.001888 , PubMed 28742009 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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