Megasphaera hexanoica (KCCM 43214, JCM 31403, MH)

Name: Megasphaera hexanoica (KCCM 43214, JCM 31403, MH)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140555

Type strain

Strain Designation MH

Culture col. no. KCCM 43214 JCM 31403 MH KFCC 11466P

NCBI tax ID(s) 1675036

Special Collections JCM Literature strains

History JCM <-- B.-I. Sang; Hanyang Univ., South Korea; KFCC 11466P.

Links

Megasphaera hexanoica MH is an anaerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from cow rumen.

Gram-negative coccus-shaped colony-forming anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 131865

JCM 31403

@ref 20215

Last LPSN update 2026-05-29 08:39:44

Domain Bacteria

Phylum Bacillota

Class Negativicutes

Order Veillonellales

Family Veillonellaceae

Genus Megasphaera

Species Megasphaera hexanoica

Full scientific name Megasphaera hexanoica Jeon et al. 2017

Synonyms (1)

"Caecibacter massiliensis"

BacDive ID	Other strains from **Megasphaera hexanoica** (1)	Type strain
159636	M. hexanoica Oil+RF-744-FAT-6-1, DSM 106893

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43304	negative	0.8-1.2 µm	0.5-0.8 µm	coccus-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43304	0.5-1 mm	slightly yellowish, convex and opaque	circular	6-7 days	mPYF

Culture and growth conditions

Culture medium

@ref	Name	Growth
43304	mPYF

Culture temp

@ref	Growth	Type	Temperature (°C)
43304	positive	growth	30-40
43304	positive	optimum	37
67770	positive	growth	37

Culture pH

@ref	Ability	Type	PH
43304	positive	optimum	6.5
43304	positive	growth	5.5-7.5

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43304	anaerobe
125438	anaerobe	90.191
125439	facultative anaerobe	92.871

Spore formation

@ref	Spore formation	Confidence
125439		93.138

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43304	30089 ChEBI	acetate	+	growth
43304	27613 ChEBI	amygdalin	-	builds acid from
43304	18305 ChEBI	arbutin	-	builds acid from
43304	29016 ChEBI	arginine	-	hydrolysis
43304	17968 ChEBI	butyrate	+	growth
43304	17057 ChEBI	cellobiose	-	builds acid from
43304	17108 ChEBI	D-arabinose	+	builds acid from
43304	18333 ChEBI	D-arabitol	+	builds acid from
43304	15824 ChEBI	D-fructose	+	builds acid from
43304	28847 ChEBI	D-fucose	-	builds acid from
43304	12936 ChEBI	D-galactose	+	assimilation
43304	17634 ChEBI	D-glucose	-	builds acid from
43304	62318 ChEBI	D-lyxose	-	builds acid from
43304	16899 ChEBI	D-mannitol	-	builds acid from
43304	16024 ChEBI	D-mannose	+	carbon source
43304	16988 ChEBI	D-ribose	-	builds acid from
43304	17924 ChEBI	D-sorbitol	-	builds acid from
43304	16443 ChEBI	D-tagatose	-	builds acid from
43304	65327 ChEBI	D-xylose	-	builds acid from
43304	17113 ChEBI	erythritol	-	builds acid from
43304	4853 ChEBI	esculin	-	hydrolysis
43304		esculin ferric citrate	-	builds acid from
43304	16813 ChEBI	galactitol	-	builds acid from
43304	28260 ChEBI	galactose	+/-	carbon source
43304	5291 ChEBI	gelatin	-	hydrolysis
43304	28066 ChEBI	gentiobiose	-	builds acid from
43304	24265 ChEBI	gluconate	+	assimilation
43304	17754 ChEBI	glycerol	-	builds acid from
43304	28087 ChEBI	glycogen	-	builds acid from
43304	15443 ChEBI	inulin	-	builds acid from
43304	30849 ChEBI	L-arabinose	-	builds acid from
43304	18403 ChEBI	L-arabitol	-	builds acid from
43304	18287 ChEBI	L-fucose	-	builds acid from
43304	62345 ChEBI	L-rhamnose	-	builds acid from
43304	17266 ChEBI	L-sorbose	-	builds acid from
43304	24996 ChEBI	lactate	+/-	carbon source
43304	17716 ChEBI	lactose	-	builds acid from
43304		maltose	-	builds acid from
43304	29864 ChEBI	mannitol	-	assimilation
43304	6731 ChEBI	melezitose	-	builds acid from
43304	28053 ChEBI	melibiose	-	builds acid from
43304	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
43304	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
43304	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
43304	17268 ChEBI	myo-inositol	+	builds acid from
43304	506227 ChEBI	N-acetylglucosamine	-	builds acid from
43304	17632 ChEBI	nitrate	-	reduction
43304		potassium 2-dehydro-D-gluconate	-	builds acid from
43304		potassium 5-dehydro-D-gluconate	+	builds acid from
43304	32032 ChEBI	potassium gluconate	+	builds acid from
43304	15361 ChEBI	pyruvate	+	assimilation
43304	16634 ChEBI	raffinose	-	builds acid from
43304	15963 ChEBI	ribitol	-	builds acid from
43304	17814 ChEBI	salicin	-	builds acid from
43304	28017 ChEBI	starch	-	builds acid from
43304	17992 ChEBI	sucrose	-	builds acid from
43304	27082 ChEBI	trehalose	-	builds acid from
43304	32528 ChEBI	turanose	-	builds acid from
43304	17151 ChEBI	xylitol	-	builds acid from

Antibiotic resistance

@ref	ChEBI	Metabolite	Is sensitive	Sensitivity conc.	Is resistant	Resistance conc.
43304	37943	colistin		10 µg (disc)
43304	28001	vancomycin				5 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite
43304	15688 ChEBI	acetoin
43304	16526 ChEBI	carbon dioxide
43304	18276 ChEBI	dihydrogen
43304	16136 ChEBI	hydrogen sulfide

Enzymes

@ref	Value	Activity	Ec
43304	catalase	-	1.11.1.6
43304	cytochrome oxidase	-	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Rumen

Isolation

@ref	Sample type
43304	cow rumen
67770	Cow rumen

Taxonmaps

69479

Global distribution of 16S sequence KX021300 (>99% sequence identity) for Veillonellaceae from Microbeatlas

Aquatic Samples	2091
Soil Samples	625
Animal Samples	42073
Plant Samples	397
Total Samples	45186

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM331577v1 assembly for Megasphaera hexanoica MH	complete	GCA_003315775	1675036.3	2841216226	1675036	97.22

16S sequences

@ref	Description	Accession	Database
43304	Megasphaera hexanoica strain MH 16S ribosomal RNA gene, partial sequence	KX021300

GC content

@ref	GC-content (mol%)	Method
43304	51.8	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM331577v1 assembly for Megasphaera hexanoica MH
NCBI: GCA_003315775

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	92.87	no
125439	motility	BacteriaNetⓘ	no	75.75	no
125439	gram_stain	BacteriaNetⓘ	positive	62.30	no
125439	spore_formation	BacteriaNetⓘ	no	93.14	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Megasphaera hexanoica MH
NCBI: GCA_003315775.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	86.47	no
125438	anaerobic	anaerobicⓘ	yes	90.19	yes
125438	aerobic	aerobicⓘ	no	96.04	yes
125438	spore-forming	spore-formingⓘ	no	79.13	no
125438	thermophilic	thermophileⓘ	no	91.67	no
125438	flagellated	motile2+ⓘ	no	88.55	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.	Jeon BS, Kim S, Sang BI	Int J Syst Evol Microbiol	10.1099/ijsem.0.001888	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43304	Byoung Seung Jeon, Seil Kim, Byoung-In Sang: Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen. IJSEM 67: 2114 - 2120 2017 ( DOI 10.1099/ijsem.0.001888 , PubMed 28742009 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Megasphaera hexanoica (KCCM 43214, JCM 31403, MH)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003315775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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