Name: Paenibacillus polysaccharolyticus BL9
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140554

Type strain:

Species: Paenibacillus polysaccharolyticus

Strain Designation: BL9

Culture col. no.: ICMP 17623, NBRC 105191

NCBI tax ID(s): 582692 (species)

Special Collections

Literature strains

Section

Paenibacillus polysaccharolyticus BL9 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from leaves of Bamboo collected from Tamilnadu Agricultural University campus.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus polysaccharolyticus**
	Full Scientific Name (LPSN)	Paenibacillus polysaccharolyticus Madhaiyan et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43305]	Gram stain	positive

[Ref.: #43305]	Cell length	2.7-3.8 µm

[Ref.: #43305]	Cell width	0.5-0.8 µm

[Ref.: #43305]	Cell shape	rod-shaped

[Ref.: #43305]	Motility	yes

[Ref.: #43305]	Colony size	0.5-1.5 mm
[Ref.: #43305]	Colony color	pale white translucent
[Ref.: #43305]	Colony shape	circular
[Ref.: #43305]	Incubation period	2 days
[Ref.: #43305]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43305]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43305]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43305]			optimum	28-30 °C
[Ref.: #43305]			growth	15-37 °C

	pH	Kind of pH		pH
[Ref.: #43305]			optimum	6.5
[Ref.: #43305]			growth	4-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43305]

Oxygen tolerance

aerobe

[Ref.: #69480]	Ability of spore formation	yes Confidence in %92.891
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43305]		NaCl		optimum	5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43305]	2,3-butanediol ChEBI	-	growth
[Ref.: #43305]	2-deoxyadenosine ChEBI	-	growth
[Ref.: #43305]	2-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43305]	2-oxobutanoate ChEBI	+	carbon source
[Ref.: #43305]	2-oxopentanoate ChEBI	-	growth
[Ref.: #43305]	3-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43305]	3-O-methyl-D-glucose ChEBI	+	carbon source
[Ref.: #43305]	4-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43305]	4-hydroxyphenylacetic acid ChEBI	+	carbon source
[Ref.: #43305]	acetate ChEBI	+	carbon source
[Ref.: #43305]	adenosine ChEBI	-	growth
[Ref.: #43305]	alpha-cyclodextrin ChEBI	+	carbon source
[Ref.: #43305]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #43305]	alpha-D-glucose ChEBI	-	growth
[Ref.: #43305]	amp ChEBI	-	growth
[Ref.: #43305]	amygdalin ChEBI	+	carbon source
[Ref.: #43305]	arbutin ChEBI	+	carbon source
[Ref.: #43305]	beta-cyclodextrin ChEBI	+	carbon source
[Ref.: #43305]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #43305]	casein	+	hydrolysis
[Ref.: #43305]	cellobiose ChEBI	+	carbon source
[Ref.: #43305]	D-alanine ChEBI	-	growth
[Ref.: #43305]	D-arabitol ChEBI	-	growth
[Ref.: #43305]	D-fructose ChEBI	+	carbon source
[Ref.: #43305]	D-fructose 6-phosphate ChEBI	-	growth
[Ref.: #43305]	D-galactose ChEBI	+	carbon source
[Ref.: #43305]	D-galacturonic acid ChEBI	-	growth
[Ref.: #43305]	D-gluconate ChEBI	-	growth
[Ref.: #43305]	D-glucose 6-phosphate ChEBI	-	growth
[Ref.: #43305]	D-malate ChEBI	-	growth
[Ref.: #43305]	D-mannitol ChEBI	+	carbon source
[Ref.: #43305]	D-mannose ChEBI	+	carbon source
[Ref.: #43305]	D-psicose ChEBI	+	carbon source
[Ref.: #43305]	D-ribose ChEBI	+	carbon source
[Ref.: #43305]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43305]	D-tagatose ChEBI	-	growth
[Ref.: #43305]	D-xylose ChEBI	+	carbon source
[Ref.: #43305]	dextrin ChEBI	+	carbon source
[Ref.: #43305]	esculin ChEBI	+	hydrolysis
[Ref.: #43305]	gelatin ChEBI	+	hydrolysis
[Ref.: #43305]	gentiobiose ChEBI	-	growth
[Ref.: #43305]	glycerol ChEBI	+	carbon source
[Ref.: #43305]	glycerol 1-phosphate ChEBI	-	growth
[Ref.: #43305]	glycogen ChEBI	-	growth
[Ref.: #43305]	glycyl-l-glutamate ChEBI	-	growth
[Ref.: #43305]	inosine ChEBI	-	growth
[Ref.: #43305]	inulin ChEBI	-	growth
[Ref.: #43305]	L-alaninamide ChEBI	-	growth
[Ref.: #43305]	L-alanine ChEBI	-	growth
[Ref.: #43305]	L-alanylglycine ChEBI	-	growth
[Ref.: #43305]	L-arabinose ChEBI	+	carbon source
[Ref.: #43305]	L-asparagine ChEBI	-	growth
[Ref.: #43305]	L-fucose ChEBI	-	growth
[Ref.: #43305]	L-glutamate ChEBI	-	growth
[Ref.: #43305]	L-malate ChEBI	-	growth
[Ref.: #43305]	L-pyroglutamic acid ChEBI	-	growth
[Ref.: #43305]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43305]	L-serine ChEBI	-	growth
[Ref.: #43305]	lactamide ChEBI	-	growth
[Ref.: #43305]	lactate ChEBI	-	growth
[Ref.: #43305]	lactose ChEBI	-	growth
[Ref.: #43305]	lactulose ChEBI	+	carbon source
[Ref.: #43305]	maltose ChEBI	+	carbon source
[Ref.: #43305]	maltotriose ChEBI	+	carbon source
[Ref.: #43305]	mannan ChEBI	-	growth
[Ref.: #43305]	melezitose ChEBI	+	carbon source
[Ref.: #43305]	melibiose ChEBI	+	carbon source
[Ref.: #43305]	methyl (R)-lactate ChEBI	-	growth
[Ref.: #43305]	methyl alpha-D-galactoside ChEBI	-	growth
[Ref.: #43305]	methyl alpha-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #43305]	methyl alpha-D-mannoside ChEBI	-	growth
[Ref.: #43305]	methyl beta-D-galactoside ChEBI	+	carbon source
[Ref.: #43305]	methyl beta-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #43305]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #43305]	monomethyl succinate ChEBI	+	carbon source
[Ref.: #43305]	myo-inositol ChEBI	-	growth
[Ref.: #43305]	N-acetyl-beta-D-mannosamine ChEBI	+	carbon source
[Ref.: #43305]	N-acetyl-L-glutamate ChEBI	+	carbon source
[Ref.: #43305]	N-acetylgalactosamine ChEBI	-	growth
[Ref.: #43305]	nitrate ChEBI	+	reduction
[Ref.: #43305]	palatinose ChEBI	+	carbon source
[Ref.: #43305]	propionate ChEBI	-	growth
[Ref.: #43305]	putrescine ChEBI	+	carbon source
[Ref.: #43305]	pyruvate ChEBI	+	carbon source
[Ref.: #43305]	raffinose ChEBI	+	carbon source
[Ref.: #43305]	salicin ChEBI	+	carbon source
[Ref.: #43305]	sedoheptulosan	+	carbon source
[Ref.: #43305]	stachyose ChEBI	-	growth
[Ref.: #43305]	succinamate ChEBI	-	growth
[Ref.: #43305]	succinate ChEBI	+	carbon source
[Ref.: #43305]	sucrose ChEBI	+	carbon source
[Ref.: #43305]	thymidine ChEBI	-	growth
[Ref.: #43305]	thymidine 5'-monophosphate ChEBI	-	growth
[Ref.: #43305]	trehalose ChEBI	+	carbon source
[Ref.: #43305]	tributyrin ChEBI	+	hydrolysis
[Ref.: #43305]	turanose ChEBI	-	growth
[Ref.: #43305]	tween 40 ChEBI	+	carbon source
[Ref.: #43305]	tween 80 ChEBI	+	carbon source
[Ref.: #43305]	uridine ChEBI	-	growth
[Ref.: #43305]	uridine 5'-monophosphate ChEBI	-	growth
[Ref.: #43305]	xylan ChEBI	+	hydrolysis
[Ref.: #43305]	xylitol ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43305]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #43305]	acid phosphatase	-	3.1.3.2
[Ref.: #43305]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43305]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43305]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43305]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43305]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43305]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43305]	beta-glucosidase	-	3.2.1.21
[Ref.: #43305]	beta-glucuronidase	+	3.2.1.31
[Ref.: #43305]	catalase	+	1.11.1.6
[Ref.: #43305]	cystine arylamidase	-	3.4.11.3
[Ref.: #43305]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43305]	esterase (C 4)	-
[Ref.: #43305]	esterase Lipase (C 8)	-
[Ref.: #43305]	leucine arylamidase	-	3.4.11.1
[Ref.: #43305]	lipase (C 14)	-
[Ref.: #43305]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43305]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43305]	trypsin	+	3.4.21.4
[Ref.: #43305]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43305]

Fatty acid	Percentage
C15:0 anteiso	53.2
C16:0 iso	16.4
C16:0	10.1
C14:0 iso	7.9
C17:0 anteiso	4.4
C15:0 iso	3.7
C14:0	2.7
C16:1ω11c	0.9
C17:0 iso	0.7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43305]	Sample type/isolated from	leaves of Bamboo (Phyllostachys aureosulcata) collected from Tamilnadu Agricultural University campus
[Ref.: #43305]	Geographic location (country and/or sea, region)	Coimbatore, India
[Ref.: #43305]	Country	India
[Ref.: #43305]	Country ISO 3 Code	IND
[Ref.: #43305]	Continent	Asia
[Ref.: #43305]	Geographic location	11.0000°/77.0000°
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU912452 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	18
Soil Samples	93
Animal Samples	40
Plant Samples	728
Total Samples	879

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43305]	Paenibacillus polysaccharolyticus strain BL9 16S ribosomal RNA gene, partial sequence	EU912452		GenBank ENA
[Ref.: #43305]	Paenibacillus polysaccharolyticus strain BL9, whole genome shotgun sequencing project	FMVM01000000		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus polysaccharolyticus BL9	GCA_900102085	scaffold	GenBank ENA	582692 tax ID	*
[Ref.: #66792]	Paenibacillus polysaccharolyticus BL9	2654588110	draft	JGI IMG/M	582692 tax ID	*

[Ref.: #43305]

GC-content

45.6 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus polysaccharolyticus BL9 NCBI: GCA_900102085.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus polysaccharolyticus BL9 NCBI: GCA_900102085.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	68.979	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	91.434	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	50.265	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	92.891	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.19	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	86.591	no

Availability in culture collections External links

[Ref.: #43305]

Culture collection no.

ICMP 17623, NBRC 105191

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata.	Madhaiyan M, Poonguzhali S, Saravanan VS, Duraipandiyan V, Al-Dhabi NA, Kwon SW, Whitman WB	Int J Syst Evol Microbiol	10.1099/ijsem.0.001901	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43305

Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Soon-Wo Kwon, William B. Whitman: Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata. IJSEM 67: 2127 - 2133 2017 ( DOI 10.1099/ijsem.0.001901 , PubMed 28671536 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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