Strain identifier

BacDive ID: 140554

Type strain: Yes

Species: Paenibacillus polysaccharolyticus

Strain Designation: BL9

NCBI tax ID(s): 582692 (species)

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General

@ref: 43305

BacDive-ID: 140554

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Paenibacillus polysaccharolyticus BL9 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from leaves of Bamboo collected from Tamilnadu Agricultural University campus.

NCBI tax id

  • NCBI tax id: 582692
  • Matching level: species

doi: 10.13145/bacdive140554.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus polysaccharolyticus
  • full scientific name: Paenibacillus polysaccharolyticus Madhaiyan et al. 2017

@ref: 43305

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus polysaccharolyticus

strain designation: BL9

type strain: yes

Morphology

cell morphology

  • @ref: 43305
  • gram stain: positive
  • cell length: 2.7-3.8 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43305
  • colony size: 0.5-1.5 mm
  • colony color: pale white translucent
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43305
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperature
43305positiveoptimum28-30
43305positivegrowth15-37

culture pH

@refabilitytypepHPH range
43305positivegrowth4-10alkaliphile
43305positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43305
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69480yes92.891
69481yes100

halophily

  • @ref: 43305
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4330537166xylan+hydrolysis
4330535020tributyrin+hydrolysis
433055291gelatin+hydrolysis
43305casein+hydrolysis
4330540585alpha-cyclodextrin+carbon source
43305495055beta-cyclodextrin+carbon source
4330523652dextrin+carbon source
4330553423tween 40+carbon source
4330553426tween 80+carbon source
4330563154N-acetyl-beta-D-mannosamine+carbon source
4330527613amygdalin+carbon source
4330530849L-arabinose+carbon source
4330518305arbutin+carbon source
4330517057cellobiose+carbon source
4330515824D-fructose+carbon source
4330512936D-galactose+carbon source
4330517925alpha-D-glucose+carbon source
433056359lactulose+carbon source
4330517306maltose+carbon source
4330561993maltotriose+carbon source
4330516899D-mannitol+carbon source
4330516024D-mannose+carbon source
433056731melezitose+carbon source
4330528053melibiose+carbon source
4330518394palatinose+carbon source
4330527605D-psicose+carbon source
4330516634raffinose+carbon source
4330562345L-rhamnose+carbon source
4330516988D-ribose+carbon source
4330517814salicin+carbon source
4330517754glycerol+carbon source
43305sedoheptulosan+carbon source
4330517924D-sorbitol+carbon source
4330517992sucrose+carbon source
4330527082trehalose+carbon source
4330565327D-xylose+carbon source
4330530089acetate+carbon source
43305645522-hydroxybutyrate+carbon source
43305370543-hydroxybutyrate+carbon source
43305167244-hydroxybutyrate+carbon source
43305181014-hydroxyphenylacetic acid+carbon source
4330530031succinate+carbon source
4330517148putrescine+carbon source
4330544337N-acetyl-L-glutamate+carbon source
43305167632-oxobutanoate+carbon source
4330551850methyl pyruvate+carbon source
4330575146monomethyl succinate+carbon source
4330515361pyruvate+carbon source
4330528087glycogen-growth
4330515443inulin-growth
4330528808mannan-growth
4330528037N-acetylgalactosamine-growth
4330518333D-arabitol-growth
4330518287L-fucose-growth
4330518024D-galacturonic acid-growth
4330528066gentiobiose-growth
433058391D-gluconate-growth
4330517268myo-inositol-growth
4330517716lactose-growth
4330555507methyl alpha-D-galactoside-growth
4330543943methyl alpha-D-mannoside-growth
4330517164stachyose-growth
4330516443D-tagatose-growth
4330532528turanose-growth
4330517151xylitol-growth
4330575144lactamide-growth
4330524996lactate-growth
4330517272propionate-growth
43305143136succinamate-growth
4330521217L-alaninamide-growth
4330515570D-alanine-growth
4330516977L-alanine-growth
4330573786L-alanylglycine-growth
4330517196L-asparagine-growth
4330529985L-glutamate-growth
4330518183L-pyroglutamic acid-growth
4330517596inosine-growth
4330517748thymidine-growth
4330517115L-serine-growth
43305620642,3-butanediol-growth
4330516335adenosine-growth
43305172562-deoxyadenosine-growth
4330514314D-glucose 6-phosphate-growth
4330516704uridine-growth
4330578697D-fructose 6-phosphate-growth
4330563528thymidine 5'-monophosphate-growth
433054853esculin+hydrolysis
4330585146carboxymethylcellulose+hydrolysis
4330517632nitrate+reduction
43305286442-oxopentanoate-growth
4330515588D-malate-growth
4330515589L-malate-growth
4330574611methyl (R)-lactate-growth
4330573784glycyl-l-glutamate-growth
4330517540methyl beta-D-galactoside+carbon source
43305739183-O-methyl-D-glucose+carbon source
43305320061methyl alpha-D-glucopyranoside+carbon source
43305320055methyl beta-D-glucopyranoside+carbon source
4330516027amp-growth
4330516695uridine 5'-monophosphate-growth
4330517925alpha-D-glucose-growth
4330514336glycerol 1-phosphate-growth

enzymes

@refvalueactivityec
43305trypsin+3.4.21.4
43305alpha-chymotrypsin+3.4.21.1
43305naphthol-AS-BI-phosphohydrolase+
43305beta-glucuronidase+3.2.1.31
43305alpha-mannosidase+3.2.1.24
43305alkaline phosphatase-3.1.3.1
43305esterase (C 4)-
43305esterase Lipase (C 8)-
43305lipase (C 14)-
43305alpha-fucosidase-3.2.1.51
43305leucine arylamidase-3.4.11.1
43305valine arylamidase-
43305cystine arylamidase-3.4.11.3
43305acid phosphatase-3.1.3.2
43305alpha-galactosidase-3.2.1.22
433056-phospho-beta-galactosidase-3.2.1.85
43305alpha-glucosidase-3.2.1.20
43305beta-glucosidase-3.2.1.21
43305N-acetyl-beta-glucosaminidase-3.2.1.52
43305catalase+1.11.1.6
43305cytochrome oxidase+1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43305C14:02.7
    43305C16:010.1
    43305C14:0 iso7.9
    43305C15:0 anteiso53.2
    43305C15:0 iso3.7
    43305C16:0 iso16.4
    43305C17:0 iso0.7
    43305C17:0 anteiso4.4
    43305C16:1ω11c0.9
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • incubation pH: 6.00
  • incubation_oxygen: aerobic
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 43305
  • sample type: leaves of Bamboo (Phyllostachys aureosulcata) collected from Tamilnadu Agricultural University campus
  • geographic location: Coimbatore, India
  • country: India
  • origin.country: IND
  • continent: Asia
  • latitude: 11
  • longitude: 77

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Leaf (Phyllosphere)

taxonmaps

  • @ref: 69479
  • File name: preview.99_24721.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_141;97_15017;98_18591;99_24721&stattab=map
  • Last taxonomy: Paenibacillus
  • 16S sequence: EU912452
  • Sequence Identity:
  • Total samples: 879
  • soil counts: 93
  • aquatic counts: 18
  • animal counts: 40
  • plant counts: 728

Sequence information

16S sequences

@refdescriptionaccessiondatabase
43305Paenibacillus polysaccharolyticus strain BL9 16S ribosomal RNA gene, partial sequenceEU912452nuccore
43305Paenibacillus polysaccharolyticus strain BL9, whole genome shotgun sequencing projectFMVM01000000nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus polysaccharolyticus BL9GCA_900102085scaffoldncbi582692
66792Paenibacillus polysaccharolyticus BL92654588110draftimg582692

GC content

  • @ref: 43305
  • GC-content: 45.6
  • method: genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes68.979yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no91.434no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes50.265yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes92.891no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.19no
69480flagellatedmotile2+Ability to perform flagellated movementyes86.591no

External links

@ref: 43305

culture collection no.: ICMP 17623, NBRC 105191

literature

  • topic: Phylogeny
  • Pubmed-ID: 28671536
  • title: Paenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata.
  • authors: Madhaiyan M, Poonguzhali S, Saravanan VS, Duraipandiyan V, Al-Dhabi NA, Kwon SW, Whitman WB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001901
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, Plant Leaves/*microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43305Munusamy Madhaiyan, Selvaraj Poonguzhali, Venkatakrishnan Sivaraj Saravanan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Soon-Wo Kwon, William B. WhitmanPaenibacillus polysaccharolyticus sp. nov., a xylanolytic and cellulolytic bacteria isolated from leaves of Bamboo Phyllostachys aureosulcata10.1099/ijsem.0.001901IJSEM 67: 2127-2133 201728671536
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1