Strain identifier

BacDive ID: 140555

Type strain: Yes

Species: Megasphaera hexanoica

Strain Designation: MH

Strain history: B.-I. Sang; Hanyang Univ., South Korea; KFCC 11466P.

NCBI tax ID(s): 1675036 (species)

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General

@ref: 43304

BacDive-ID: 140555

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-negative, coccus-shaped, colony-forming

description: Megasphaera hexanoica MH is an anaerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from cow rumen.

NCBI tax id

  • NCBI tax id: 1675036
  • Matching level: species

strain history

  • @ref: 67770
  • history: B.-I. Sang; Hanyang Univ., South Korea; KFCC 11466P.

doi: 10.13145/bacdive140555.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Veillonellales
  • family: Veillonellaceae
  • genus: Megasphaera
  • species: Megasphaera hexanoica
  • full scientific name: Megasphaera hexanoica Jeon et al. 2017

@ref: 43304

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Veillonellales

family: Veillonellaceae

genus: Megasphaera

species: Megasphaera hexanoica

strain designation: MH

type strain: yes

Morphology

cell morphology

  • @ref: 43304
  • gram stain: negative
  • cell length: 0.8-1.2 µm
  • cell width: 0.5-0.8 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 43304
  • colony size: 0.5-1 mm
  • colony color: slightly yellowish, convex and opaque
  • colony shape: circular
  • incubation period: 6-7 days
  • medium used: mPYF

Culture and growth conditions

culture medium

  • @ref: 43304
  • name: mPYF
  • growth: yes

culture temp

@refgrowthtypetemperature
43304positivegrowth30-40
43304positiveoptimum37
67770positivegrowth37

culture pH

@refabilitytypepH
43304positivegrowth5.5-7.5
43304positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
43304anaerobe
69480anaerobe90.191

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4330417108D-arabinose+builds acid from
4330415824D-fructose+builds acid from
4330418333D-arabitol+builds acid from
4330417268myo-inositol+builds acid from
4330432032potassium gluconate+builds acid from
43304potassium 5-dehydro-D-gluconate+builds acid from
4330417754glycerol-builds acid from
4330417113erythritol-builds acid from
4330430849L-arabinose-builds acid from
4330416988D-ribose-builds acid from
4330465327D-xylose-builds acid from
4330415963ribitol-builds acid from
4330474863methyl beta-D-xylopyranoside-builds acid from
4330417634D-glucose-builds acid from
4330417266L-sorbose-builds acid from
4330462345L-rhamnose-builds acid from
4330416813galactitol-builds acid from
4330416899D-mannitol-builds acid from
4330417924D-sorbitol-builds acid from
43304320061methyl alpha-D-glucopyranoside-builds acid from
43304506227N-acetylglucosamine-builds acid from
4330427613amygdalin-builds acid from
4330418305arbutin-builds acid from
43304esculin ferric citrate-builds acid from
4330417814salicin-builds acid from
4330417057cellobiose-builds acid from
43304maltose-builds acid from
4330417716lactose-builds acid from
4330428053melibiose-builds acid from
4330417992sucrose-builds acid from
4330427082trehalose-builds acid from
4330415443inulin-builds acid from
433046731melezitose-builds acid from
4330416634raffinose-builds acid from
4330428017starch-builds acid from
4330428087glycogen-builds acid from
4330417151xylitol-builds acid from
4330428066gentiobiose-builds acid from
4330432528turanose-builds acid from
4330416443D-tagatose-builds acid from
4330462318D-lyxose-builds acid from
4330428847D-fucose-builds acid from
4330418287L-fucose-builds acid from
4330418403L-arabitol-builds acid from
43304potassium 2-dehydro-D-gluconate-builds acid from
4330443943methyl alpha-D-mannoside-builds acid from
4330416024D-mannose+carbon source
4330424996lactate+/-carbon source
4330428260galactose+/-carbon source
4330430089acetate+growth
4330417968butyrate+growth
4330417632nitrate-reduction
433044853esculin-hydrolysis
433045291gelatin-hydrolysis
4330429016arginine-hydrolysis
4330412936D-galactose+assimilation
4330429864mannitol-assimilation
4330424265gluconate+assimilation
4330415361pyruvate+assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4330437943colistinyesyes10 µg (disc)
4330428001vancomycinyesyes5 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4330416136hydrogen sulfideyes
4330415688acetoinno
4330416526carbon dioxideyes
4330418276dihydrogenyes

enzymes

@refvalueactivityec
43304cytochrome oxidase-1.9.3.1
43304catalase-1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample type
43304cow rumen
67770Cow rumen

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Rumen

taxonmaps

  • @ref: 69479
  • File name: preview.99_4696.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_37;96_2331;97_2834;98_3521;99_4696&stattab=map
  • Last taxonomy: Veillonellaceae
  • 16S sequence: KX021300
  • Sequence Identity:
  • Total samples: 45186
  • soil counts: 625
  • aquatic counts: 2091
  • animal counts: 42073
  • plant counts: 397

Sequence information

16S sequences

  • @ref: 43304
  • description: Megasphaera hexanoica strain MH 16S ribosomal RNA gene, partial sequence
  • accession: KX021300
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Megasphaera hexanoica MHGCA_003315775completencbi1675036
66792Megasphaera hexanoica MH2841216226completeimg1675036

GC content

  • @ref: 43304
  • GC-content: 51.8
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno86.468no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes90.191yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no96.037yes
69480spore-formingspore-formingAbility to form endo- or exosporesno79.128no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno91.672no
69480flagellatedmotile2+Ability to perform flagellated movementno88.548yes

External links

@ref: 43304

culture collection no.: KCCM 43214, JCM 31403, KFCC 11466P

literature

  • topic: Phylogeny
  • Pubmed-ID: 28742009
  • title: Megasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen.
  • authors: Jeon BS, Kim S, Sang BI
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001888
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Carboxylic Acids/*metabolism, Cattle/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Megasphaera/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rumen/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43304Byoung Seung Jeon, Seil Kim, Byoung-In SangMegasphaera hexanoica sp. nov., a medium-chain carboxylic acid-producing bacterium isolated from a cow rumen10.1099/ijsem.0.001888IJSEM 67: 2114-2120 201728742009
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes