Name: Metabacillus mangrovi AK61
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140556

Type strain:

Species: Metabacillus mangrovi

Strain Designation: AK61

Culture col. no.: JCM 31087, MTCC 12015, KCTC 33872

Strain history: <- P. Anil Kumar, CSIR-IMTECH, Institute of Microbial Technology, India

NCBI tax ID(s): 1491830 (species)

Special Collections

Section

Metabacillus mangrovi AK61 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from sediment sample collected from Coringa mangrove forest.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Metabacillus*
	Species	**Metabacillus mangrovi**
	Full Scientific Name (LPSN)	Metabacillus mangrovi (Gupta et al. 2017) Gupta et al. 2020

Synonym

Bacillus mangrovi

Information on morphological and physiological properties Morphology

[Ref.: #43306]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive

[Ref.: #43306]	Cell length	3-3.5 µm

[Ref.: #43306]	Cell width	0.8-1 µm

[Ref.: #43306]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43306]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #43306]	Colony size	1.5 mm
[Ref.: #43306]	Colony color	yellowish-orange, opaque, shiny
[Ref.: #43306]	Colony shape	circular
[Ref.: #43306]	Incubation period	1 day
[Ref.: #43306]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43306]

Culture medium

Marine agar (MA)

[Ref.: #43306]

Culture medium growth

		Temperature
[Ref.: #43306]	optimum	25-37 °C
[Ref.: #43306]	growth	15-42 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	25-37 °C

	pH	Kind of pH		pH
[Ref.: #43306]			optimum	7
[Ref.: #43306]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43306]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43306]	Spore description	central endospore position
[Ref.: #43306]	Type of spore	endospore
[Ref.: #43306]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %94.753
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43306]		NaCl		growth	3	%
[Ref.: #43306]		NaCl		optimum	0-1	%

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43306]	(R)-amygdalin ChEBI	-	assimilation
[Ref.: #43306]	agar ChEBI	-	hydrolysis
[Ref.: #43306]	arabinitol ChEBI	-	assimilation
[Ref.: #43306]	casein	-	hydrolysis
[Ref.: #43306]	cellobiose ChEBI	-	assimilation
[Ref.: #43306]	cellulose ChEBI	-	hydrolysis
[Ref.: #43306]	citrate ChEBI	-	assimilation
[Ref.: #43306]	cyclodextrin ChEBI	+	assimilation
[Ref.: #43306]	D-arabinose ChEBI	-	assimilation
[Ref.: #43306]	D-galactose ChEBI	-	assimilation
[Ref.: #43306]	D-glucose ChEBI	-	assimilation
[Ref.: #43306]	D-mannitol ChEBI	-	assimilation
[Ref.: #43306]	D-mannose ChEBI	-	assimilation
[Ref.: #43306]	D-ribose ChEBI	-	assimilation
[Ref.: #43306]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43306]	D-xylose ChEBI	-	assimilation
[Ref.: #43306]	dna ChEBI	-	hydrolysis
[Ref.: #43306]	erythritol ChEBI	-	assimilation
[Ref.: #43306]	esculin ChEBI	-	hydrolysis
[Ref.: #43306]	fructose ChEBI	-	assimilation
[Ref.: #43306]	galactitol ChEBI	-	assimilation
[Ref.: #43306]	galactose ChEBI	-	assimilation
[Ref.: #43306]	gelatin ChEBI	-	hydrolysis
[Ref.: #43306]	glycerol ChEBI	-	assimilation
[Ref.: #43306]	inulin ChEBI	-	assimilation
[Ref.: #43306]	L-arabinose ChEBI	-	assimilation
[Ref.: #43306]	lactose ChEBI	-	assimilation
[Ref.: #43306]	malonate ChEBI	-	assimilation
[Ref.: #43306]	maltose ChEBI	-	assimilation
[Ref.: #43306]	mannitol ChEBI	-	assimilation
[Ref.: #43306]	mannose ChEBI	-	assimilation
[Ref.: #43306]	melibiose ChEBI	+	assimilation
[Ref.: #43306]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43306]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #43306]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #43306]	myo-inositol ChEBI	-	assimilation
[Ref.: #43306]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43306]	nitrate ChEBI	-	reduction
[Ref.: #43306]	pullulan ChEBI	-	assimilation
[Ref.: #43306]	raffinose ChEBI	-	assimilation
[Ref.: #43306]	rhamnose ChEBI	-	assimilation
[Ref.: #43306]	ribitol ChEBI	-	assimilation
[Ref.: #43306]	salicin ChEBI	-	assimilation
[Ref.: #43306]	sodium gluconate ChEBI	-	assimilation
[Ref.: #43306]	sorbitol ChEBI	-	assimilation
[Ref.: #43306]	sorbose ChEBI	-	assimilation
[Ref.: #43306]	starch ChEBI	+	hydrolysis
[Ref.: #43306]	sucrose ChEBI	-	assimilation
[Ref.: #43306]	trehalose ChEBI	-	assimilation
[Ref.: #43306]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43306]	urea ChEBI	-	hydrolysis
[Ref.: #43306]	xylitol ChEBI	-	assimilation
[Ref.: #43306]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43306]	amoxicillin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	ampicillin ChEBI	yes	25	µg (disc)
[Ref.: #43306]	bacitracin ChEBI	yes	8	µg (disc)
[Ref.: #43306]	cefadroxil ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	cefprozil ChEBI	yes	30	µg (disc)
[Ref.: #43306]	ceftazidime ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cephalexin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cephalosporin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43306]	chlortetracyclin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cinoxacin ChEBI	yes	100	µg (disc)
[Ref.: #43306]	ciprofloxacin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	clarithromycin ChEBI	yes	15	µg (disc)
[Ref.: #43306]	enoxacin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	enrofloxacin ChEBI				yes	5	µg (disc)
[Ref.: #43306]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	lincomycin ChEBI				yes	2	µg (disc)
[Ref.: #43306]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	optochin ChEBI				yes
[Ref.: #43306]	penicillin g ChEBI	yes	2	Unit
[Ref.: #43306]	polymyxin b ChEBI	yes	300	µg (disc)
[Ref.: #43306]	spectinomycin ChEBI	yes	100	µg (disc)
[Ref.: #43306]	streptomycin ChEBI	yes	25	µg (disc)
[Ref.: #43306]	sulfamethizole ChEBI				yes	300	µg (disc)
[Ref.: #43306]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43306]	vancomycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	vibriostat ChEBI				yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43306]		hydrogen sulfide ChEBI	no
[Ref.: #43306]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43306]	alanine arylamidase	-	3.4.11.2
[Ref.: #43306]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43306]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43306]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43306]	arginine decarboxylase	-	4.1.1.19
[Ref.: #43306]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43306]	beta-galactopyranosidase	+
[Ref.: #43306]	beta-galactosidase	+	3.2.1.23
[Ref.: #43306]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43306]	catalase	-	1.11.1.6
[Ref.: #43306]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43306]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #43306]	leucine arylamidase	-	3.4.11.1
[Ref.: #43306]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43306]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43306]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #43306]	phosphatase	-
[Ref.: #43306]	phosphatidylinositol phospholipase C	-	3.1.4.11
[Ref.: #43306]	proline-arylamidase	-	3.4.11.5
[Ref.: #43306]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #43306]	tyrosine arylamidase	-
[Ref.: #43306]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43306]

Fatty acid	Percentage
C15:0 anteiso	40.4
C15:0 iso	23.8
C14:0 iso	15.9
C16:0 iso	7.2
C17:0 anteiso	4
C16:0	2.6
C14:0	1.3
C16:0 anteiso	1.2
C16:1ωc alcohol	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43306]	Sample type/isolated from	sediment sample collected from Coringa mangrove forest
[Ref.: #43306]	Geographic location (country and/or sea, region)	Andhra Pradesh, Coringa mangrove forest
[Ref.: #43306]	Country	India
[Ref.: #43306]	Country ISO 3 Code	IND
[Ref.: #43306]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sediment from Coringa mangrove forest
[Ref.: #67770]	Geographic location (country and/or sea, region)	Andhra Pradesh
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From sediment sample from mangrove
[Ref.: #67771]	Geographic location (country and/or sea, region)	Kakinada, Andhra Pradesh
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Forest

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG974242 (>99% sequence identity) for Bacillus mangrovi subclade from Microbeatlas

Aquatic Samples	36
Soil Samples	26
Animal Samples	17
Plant Samples	4
Total Samples	83

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43306]	Bacillus sp. AK61 partial 16S rRNA gene, strain AK61	HG974242		GenBank ENA

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Metabacillus mangrovi KCTC 33872	GCA_009711125	contig	GenBank ENA	1491830 tax ID
[Ref.: #66792]	Bacillus mangrovi strain KCTC 33872	1491830.4	wgs	PATRIC	1491830 tax ID	*

[Ref.: #67771]	GC-content	44.0 mol%
[Ref.: #67770]	GC-content	44 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus mangrovi strain KCTC 33872 PATRIC: 1491830.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus mangrovi strain KCTC 33872 PATRIC: 1491830.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	80.281	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	91.816	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	94.753	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	77.502	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	90.334	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.209	no

Availability in culture collections External links

[Ref.: #43306]

Culture collection no.

JCM 31087, MTCC 12015, KCTC 33872

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel.	Cai H, Huang Y, Liu Y, Yang L, Zhou Y, Yang S, Chen X, Jiang C, Jiang M, Jiang Y, Singh P	Int J Syst Evol Microbiol	10.1099/ijsem.0.005538	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43306

Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka: Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest. IJSEM 67: 2219 - 2224 2017 ( DOI 10.1099/ijsem.0.001928 , PubMed 28714845 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Bacillus mangrovi in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Bacillus mangrovi strain KCTC 33872

Bacillus mangrovi strain KCTC 33872

Change proposal

Change proposal