Name: Metabacillus mangrovi AK61
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140556

Type strain:

Species: Metabacillus mangrovi

Strain Designation: AK61

Culture col. no.: JCM 31087, MTCC 12015, KCTC 33872

Strain history: <- P. Anil Kumar, CSIR-IMTECH, Institute of Microbial Technology, India

NCBI tax ID(s): 1491830 (species)

Section

Metabacillus mangrovi AK61 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from sediment sample collected from Coringa mangrove forest.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Metabacillus*
	Species	**Metabacillus mangrovi**
	Full Scientific Name (LPSN)	Metabacillus mangrovi (Gupta et al. 2017) Gupta et al. 2020

Synonym

Bacillus mangrovi

Information on morphological and physiological properties Morphology

[Ref.: #43306]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43306]	Cell length	3-3.5 µm

[Ref.: #43306]	Cell width	0.8-1 µm

[Ref.: #43306]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43306]	Motility	yes
[Ref.: #67771]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.679

[Ref.: #43306]	Colony size	1.5 mm
[Ref.: #43306]	Colony color	yellowish-orange, opaque, shiny
[Ref.: #43306]	Colony shape	circular
[Ref.: #43306]	Incubation period	1 day
[Ref.: #43306]	Cultivation medium used	marine agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43306]

Culture medium

Marine agar (MA)

[Ref.: #43306]

Culture medium growth

		Temperature
[Ref.: #43306]	optimum	25-37 °C
[Ref.: #43306]	growth	15-42 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	25-37 °C

[Ref.: #43306]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43306]			optimum	7
[Ref.: #43306]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43306]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43306]	Spore description	central endospore position
[Ref.: #43306]	Type of spore	endospore
[Ref.: #43306]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	endospore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43306]		NaCl		growth	3	%
[Ref.: #43306]		NaCl		optimum	0-1	%

[Ref.: #67770]	Observation	quinones: MK-7
[Ref.: #67771]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43306]	(R)-amygdalin ChEBI	-	assimilation
[Ref.: #43306]	agar ChEBI	-	hydrolysis
[Ref.: #43306]	arabinitol ChEBI	-	assimilation
[Ref.: #43306]	casein	-	hydrolysis
[Ref.: #43306]	cellobiose ChEBI	-	assimilation
[Ref.: #43306]	cellulose ChEBI	-	hydrolysis
[Ref.: #43306]	citrate ChEBI	-	assimilation
[Ref.: #43306]	cyclodextrin ChEBI	+	assimilation
[Ref.: #43306]	D-arabinose ChEBI	-	assimilation
[Ref.: #43306]	D-galactose ChEBI	-	assimilation
[Ref.: #43306]	D-glucose ChEBI	-	assimilation
[Ref.: #43306]	D-mannitol ChEBI	-	assimilation
[Ref.: #43306]	D-mannose ChEBI	-	assimilation
[Ref.: #43306]	D-ribose ChEBI	-	assimilation
[Ref.: #43306]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43306]	D-xylose ChEBI	-	assimilation
[Ref.: #43306]	dna ChEBI	-	hydrolysis
[Ref.: #43306]	erythritol ChEBI	-	assimilation
[Ref.: #43306]	esculin ChEBI	-	hydrolysis
[Ref.: #43306]	fructose ChEBI	-	assimilation
[Ref.: #43306]	galactitol ChEBI	-	assimilation
[Ref.: #43306]	galactose ChEBI	-	assimilation
[Ref.: #43306]	gelatin ChEBI	-	hydrolysis
[Ref.: #43306]	glycerol ChEBI	-	assimilation
[Ref.: #43306]	inulin ChEBI	-	assimilation
[Ref.: #43306]	L-arabinose ChEBI	-	assimilation
[Ref.: #43306]	lactose ChEBI	-	assimilation
[Ref.: #43306]	malonate ChEBI	-	assimilation
[Ref.: #43306]	maltose ChEBI	-	assimilation
[Ref.: #43306]	mannitol ChEBI	-	assimilation
[Ref.: #43306]	mannose ChEBI	-	assimilation
[Ref.: #43306]	melibiose ChEBI	+	assimilation
[Ref.: #43306]	methyl alpha-D-mannoside ChEBI	-	assimilation
[Ref.: #43306]	methyl beta-D-glucopyranoside ChEBI	-	assimilation
[Ref.: #43306]	methyl D-glucoside ChEBI	-	assimilation
[Ref.: #43306]	myo-inositol ChEBI	-	assimilation
[Ref.: #43306]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43306]	nitrate ChEBI	-	reduction
[Ref.: #43306]	pullulan ChEBI	-	assimilation
[Ref.: #43306]	raffinose ChEBI	-	assimilation
[Ref.: #43306]	rhamnose ChEBI	-	assimilation
[Ref.: #43306]	ribitol ChEBI	-	assimilation
[Ref.: #43306]	salicin ChEBI	-	assimilation
[Ref.: #43306]	sodium gluconate ChEBI	-	assimilation
[Ref.: #43306]	sorbitol ChEBI	-	assimilation
[Ref.: #43306]	sorbose ChEBI	-	assimilation
[Ref.: #43306]	starch ChEBI	+	hydrolysis
[Ref.: #43306]	sucrose ChEBI	-	assimilation
[Ref.: #43306]	trehalose ChEBI	-	assimilation
[Ref.: #43306]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43306]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43306]	urea ChEBI	-	hydrolysis
[Ref.: #43306]	xylitol ChEBI	-	assimilation
[Ref.: #43306]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43306]	amoxicillin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	ampicillin ChEBI	yes	25	µg (disc)
[Ref.: #43306]	bacitracin ChEBI	yes	8	µg (disc)
[Ref.: #43306]	cefadroxil ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	cefprozil ChEBI	yes	30	µg (disc)
[Ref.: #43306]	ceftazidime ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cephalexin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cephalosporin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43306]	chlortetracyclin ChEBI				yes	30	µg (disc)
[Ref.: #43306]	cinoxacin ChEBI	yes	100	µg (disc)
[Ref.: #43306]	ciprofloxacin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	clarithromycin ChEBI	yes	15	µg (disc)
[Ref.: #43306]	enoxacin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	enrofloxacin ChEBI				yes	5	µg (disc)
[Ref.: #43306]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43306]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	lincomycin ChEBI				yes	2	µg (disc)
[Ref.: #43306]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	optochin ChEBI				yes
[Ref.: #43306]	penicillin g ChEBI	yes	2	Unit
[Ref.: #43306]	polymyxin b ChEBI	yes	300	µg (disc)
[Ref.: #43306]	spectinomycin ChEBI	yes	100	µg (disc)
[Ref.: #43306]	streptomycin ChEBI	yes	25	µg (disc)
[Ref.: #43306]	sulfamethizole ChEBI				yes	300	µg (disc)
[Ref.: #43306]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43306]	vancomycin ChEBI	yes	30	µg (disc)
[Ref.: #43306]	vibriostat ChEBI				yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43306]		hydrogen sulfide ChEBI	no
[Ref.: #43306]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43306]	alanine arylamidase	-	3.4.11.2
[Ref.: #43306]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43306]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43306]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43306]	arginine decarboxylase	-	4.1.1.19
[Ref.: #43306]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43306]	beta-galactopyranosidase	+
[Ref.: #43306]	beta-galactosidase	+	3.2.1.23
[Ref.: #43306]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43306]	catalase	-	1.11.1.6
[Ref.: #43306]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43306]	L-aspartate arylamidase	-	3.4.11.21
[Ref.: #43306]	leucine arylamidase	-	3.4.11.1
[Ref.: #43306]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43306]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43306]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #43306]	phosphatase	-
[Ref.: #43306]	phosphatidylinositol phospholipase C	-	3.1.4.11
[Ref.: #43306]	proline-arylamidase	-	3.4.11.5
[Ref.: #43306]	pyrrolidonyl arylamidase	-	3.4.19.3
[Ref.: #43306]	tyrosine arylamidase	-
[Ref.: #43306]	urease	-	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43306]

Fatty acid	Percentage
C15:0 anteiso	40.4
C15:0 iso	23.8
C14:0 iso	15.9
C16:0 iso	7.2
C17:0 anteiso	4
C16:0	2.6
C14:0	1.3
C16:0 anteiso	1.2
C16:1ωc alcohol	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43306]	Sample type/isolated from	sediment sample collected from Coringa mangrove forest
[Ref.: #43306]	Geographic location (country and/or sea, region)	Andhra Pradesh, Coringa mangrove forest
[Ref.: #43306]	Country	India
[Ref.: #43306]	Country ISO 3 Code	IND
[Ref.: #43306]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sediment from Coringa mangrove forest
[Ref.: #67770]	Geographic location (country and/or sea, region)	Andhra Pradesh
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From sediment sample from mangrove
[Ref.: #67771]	Geographic location (country and/or sea, region)	Kakinada, Andhra Pradesh
[Ref.: #67771]	Country	India
[Ref.: #67771]	Country ISO 3 Code	IND
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Mangrove
#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Forest

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HG974242 (>99% sequence identity) for Bacillus mangrovi subclade from Microbeatlas

Aquatic Samples	36
Soil Samples	26
Animal Samples	17
Plant Samples	4
Total Samples	83

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43306]	16S rRNA	HG974242		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Metabacillus mangrovi KCTC 33872	GCA_009711125	contig	GenBank	1491830 tax ID
[Ref.: #66792]	Bacillus mangrovi strain KCTC 33872	1491830.4	wgs	PATRIC	1491830 tax ID	*

[Ref.: #67771]	GC-content	44.0 mol%
[Ref.: #67770]	GC-content	44 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus mangrovi strain KCTC 33872 PATRIC: 1491830.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus mangrovi strain KCTC 33872 PATRIC: 1491830.4
[Ref.: #69480]	motile	yes	88.845	yes
[Ref.: #69480]	gram-positive	yes	92.455	yes
[Ref.: #69480]	anaerobic	no	98.331	no
[Ref.: #69480]	aerobic	yes	93.156	no
[Ref.: #69480]	halophile	no	57.585	no
[Ref.: #69480]	spore-forming	yes	95.97	no
[Ref.: #69480]	thermophile	no	97.199	no
[Ref.: #69480]	glucose-util	yes	87.12	yes
[Ref.: #69480]	flagellated	yes	76.062	no
[Ref.: #69480]	glucose-ferment	no	93.545	no

Availability in culture collections External links

[Ref.: #43306]

Culture collection no.

JCM 31087, MTCC 12015, KCTC 33872

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel.	Cai H, Huang Y, Liu Y, Yang L, Zhou Y, Yang S, Chen X, Jiang C, Jiang M, Jiang Y, Singh P	Int J Syst Evol Microbiol	10.1099/ijsem.0.005538	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43306

Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar Pinnaka: Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest. IJSEM 67: 2219 - 2224 2017 ( DOI 10.1099/ijsem.0.001928 , PubMed 28714845 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Bacillus mangrovi strain KCTC 33872

Bacillus mangrovi strain KCTC 33872

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