Strain identifier

BacDive ID: 140556

Type strain: Yes

Species: Metabacillus mangrovi

Strain Designation: AK61

Strain history: <- P. Anil Kumar, CSIR-IMTECH, Institute of Microbial Technology, India

NCBI tax ID(s): 1491830 (species)

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General

@ref: 43306

BacDive-ID: 140556

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Metabacillus mangrovi AK61 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from sediment sample collected from Coringa mangrove forest.

NCBI tax id

  • NCBI tax id: 1491830
  • Matching level: species

strain history

@refhistory
67770P. Anil Kumar; MTCC, India; AK61.
67771<- P. Anil Kumar, CSIR-IMTECH, Institute of Microbial Technology, India

doi: 10.13145/bacdive140556.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Metabacillus
  • species: Metabacillus mangrovi
  • full scientific name: Metabacillus mangrovi (Gupta et al. 2017) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus mangrovi

@ref: 43306

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus mangrovi

strain designation: AK61

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43306positive3-3.5 µm0.8-1 µmrod-shapedyes
67771rod-shapedyes
67771positive

colony morphology

  • @ref: 43306
  • colony size: 1.5 mm
  • colony color: yellowish-orange, opaque, shiny
  • colony shape: circular
  • incubation period: 1 day
  • medium used: marine agar

Culture and growth conditions

culture medium

  • @ref: 43306
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperature
43306positiveoptimum25-37
43306positivegrowth15-42
67770positivegrowth28
67771positivegrowth25-37

culture pH

@refabilitytypepHPH range
43306positivegrowth6-9alkaliphile
43306positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43306facultative anaerobe
67771aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
43306central endospore positionendosporeyes
67771endosporeyes
69480yes94.753
69481yes100

halophily

@refsaltgrowthtested relationconcentration
43306NaClpositivegrowth3 %
43306NaClpositiveoptimum0-1 %

observation

@refobservation
67770quinones: MK-7
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4330617632nitrate-reduction
4330628017starch+hydrolysis
433064853esculin-hydrolysis
433062509agar-hydrolysis
43306casein-hydrolysis
4330662968cellulose-hydrolysis
4330616991dna-hydrolysis
433065291gelatin-hydrolysis
4330653424tween 20-hydrolysis
4330653423tween 40-hydrolysis
4330653425tween 60-hydrolysis
4330653426tween 80-hydrolysis
4330616199urea-hydrolysis
4330628757fructose-assimilation
4330616634raffinose-assimilation
4330617057cellobiose-assimilation
4330628053melibiose+assimilation
43306506227N-acetylglucosamine-assimilation
4330617634D-glucose-assimilation
4330629864mannitol-assimilation
4330617754glycerol-assimilation
4330616947citrate-assimilation
4330617716lactose-assimilation
4330618222xylose-assimilation
4330628260galactose-assimilation
4330630849L-arabinose-assimilation
4330637684mannose-assimilation
4330615443inulin-assimilation
4330684997sodium gluconate-assimilation
4330616813galactitol-assimilation
4330617268myo-inositol-assimilation
4330630911sorbitol-assimilation
4330615963ribitol-assimilation
4330622605arabinitol-assimilation
4330617113erythritol-assimilation
4330637657methyl D-glucoside-assimilation
4330626546rhamnose-assimilation
4330643943methyl alpha-D-mannoside-assimilation
4330617151xylitol-assimilation
4330617108D-arabinose-assimilation
4330615792malonate-assimilation
4330627922sorbose-assimilation
4330623456cyclodextrin+assimilation
4330617924D-sorbitol-assimilation
4330612936D-galactose-assimilation
4330616899D-mannitol-assimilation
4330616024D-mannose-assimilation
43306320055methyl beta-D-glucopyranoside-assimilation
4330627941pullulan-assimilation
4330617814salicin-assimilation
4330617019(R)-amygdalin-assimilation
4330616988D-ribose-assimilation
4330617306maltose-assimilation
4330617992sucrose-assimilation
4330627082trehalose-assimilation
4330665327D-xylose-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
433062676amoxicillinyesyes30 µg (disc)
4330628971ampicillinyesyes25 µg (disc)
4330628669bacitracinyesyes8 µg (disc)
433063479cefadroxilyesyes30 µg (disc)
43306474053cefazolinyesyes30 µg (disc)
433063506cefprozilyesyes30 µg (disc)
433063508ceftazidimeyesyes30 µg (disc)
433063534cephalexinyesyes30 µg (disc)
4330623066cephalosporinyesyes30 µg (disc)
4330617698chloramphenicolyesyes30 µg (disc)
4330627644chlortetracyclinyesyes30 µg (disc)
433063716cinoxacinyesyes100 µg (disc)
43306100241ciprofloxacinyesyes10 µg (disc)
433063732clarithromycinyesyes15 µg (disc)
43306157175enoxacinyesyes10 µg (disc)
4330635720enrofloxacinyesyes5 µg (disc)
4330617833gentamicinyesyes10 µg (disc)
433066104kanamycinyesyes30 µg (disc)
433066472lincomycinyesyes2 µg (disc)
433067507neomycinyesyes30 µg (disc)
4330628368novobiocinyesyes30 µg (disc)
4330686455optochinyesyes
4330618208penicillin gyesyes2 Unit
433068309polymyxin byesyes300 µg (disc)
433069215spectinomycinyesyes100 µg (disc)
4330617076streptomycinyesyes25 µg (disc)
433069331sulfamethizoleyesyes300 µg (disc)
4330627902tetracyclineyesyes30 µg (disc)
4330628001vancomycinyesyes30 µg (disc)
4330673908vibriostatyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4330635581indoleno
4330616136hydrogen sulfideno

enzymes

@refvalueactivityec
43306cytochrome oxidase-1.9.3.1
43306catalase-1.11.1.6
43306lysine decarboxylase-4.1.1.18
43306arginine decarboxylase-4.1.1.19
43306ornithine decarboxylase-4.1.1.17
43306phenylalanine deaminase-4.3.1.5
43306beta-galactosidase+3.2.1.23
43306alpha-glucosidase+3.2.1.20
43306beta-galactopyranosidase+
43306alpha-galactosidase+3.2.1.22
43306pyrrolidonyl arylamidase-3.4.19.3
43306tyrosine arylamidase-
43306leucine arylamidase-3.4.11.1
43306phosphatidylinositol phospholipase C-3.1.4.11
43306L-aspartate arylamidase-3.4.11.21
43306alanine arylamidase-3.4.11.2
43306proline-arylamidase-3.4.11.5
43306alpha-mannosidase-3.2.1.24
43306beta-glucuronidase-3.2.1.31
43306phosphatase-
43306urease-3.5.1.5
43306arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43306C14:0 iso15.9
    43306C14:01.3
    43306C15:0 iso23.8
    43306C15:0 anteiso40.4
    43306C16:1ωc alcohol1.1
    43306C16:0 iso7.2
    43306C16:0 anteiso1.2
    43306C16:02.6
    43306C17:0 anteiso4
  • type of FA analysis: whole cell analysis
  • incubation medium: marine agar
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 1
  • incubation pH: 7.00
  • incubation_oxygen: aerobic
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43306sediment sample collected from Coringa mangrove forestAndhra Pradesh, Coringa mangrove forestIndiaINDAsia
67770Sediment from Coringa mangrove forestAndhra PradeshIndiaINDAsia
67771From sediment sample from mangroveKakinada, Andhra PradeshIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Forest

taxonmaps

  • @ref: 69479
  • File name: preview.99_23342.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3218;97_4121;98_5273;99_23342&stattab=map
  • Last taxonomy: Bacillus mangrovi subclade
  • 16S sequence: HG974242
  • Sequence Identity:
  • Total samples: 83
  • soil counts: 26
  • aquatic counts: 36
  • animal counts: 17
  • plant counts: 4

Sequence information

16S sequences

  • @ref: 43306
  • description: Bacillus sp. AK61 partial 16S rRNA gene, strain AK61
  • accession: HG974242
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus mangrovi strain KCTC 338721491830.4wgspatric1491830
67770Metabacillus mangrovi KCTC 33872GCA_009711125contigncbi1491830

GC content

@refGC-contentmethod
6777144.0
6777044thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes80.281yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no91.816yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes94.753no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes77.502no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno90.334yes
69480flagellatedmotile2+Ability to perform flagellated movementyes84.209no

External links

@ref: 43306

culture collection no.: JCM 31087, MTCC 12015, KCTC 33872

literature

  • topic: Phylogeny
  • Pubmed-ID: 35998028
  • title: Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel.
  • authors: Cai H, Huang Y, Liu Y, Yang L, Zhou Y, Yang S, Chen X, Jiang C, Jiang M, Jiang Y, Singh P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.005538
  • year: 2022
  • mesh: *Bacillaceae, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizophoraceae/microbiology, Rhizosphere, Sequence Analysis, DNA, Soil, Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43306Vasundhera Gupta, Pradip Kumar Singh, Suresh Korpole, Naga Radha Srinivas Tanuku, Anil Kumar PinnakaBacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest10.1099/ijsem.0.001928IJSEM 67: 2219-2224 201728714845
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1