Name: Lactobacillus johnsonii CCUG 44519
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 136238

Type strain:

Species: Lactobacillus johnsonii

Culture col. no.: CCUG 44519, IFO 13952, LMG 9437, JCM 8794, CIP 103653, ATCC 11506, NCIMB 8795, NCIMB 8892, JCM 1101, BCRC 14004, BUCSAV 419, CCM 2935, CECT 289, NBRC 13952, VPI 11088

Strain history: CIP <- 1993, T. Fujisawa, Public Hlth. Lab., Yokohama, Japan <- T. Mitsuoka, Riken, Japan

NCBI tax ID(s): 33959 (species)

SI-ID 92273

LMG 9437, ATCC 11506, CCUG 44519, IFO 13952, JCM 8794, NCFB 1693, NCIMB 8795, NCIB 8795, NCIB 8892, CIP 103653, JCM 1101, CCRC 14004, CECT 289, NCIMB 8892, NCDO1693, CCM 2935, VKM B-1919, CCRC 12294, KCTC 1120, NBRC 13952, BCRC 14004, NRRL B-2178

Other strains from Lactobacillus johnsonii

L. johnsonii DSM 10533, ATCC 33200, NCFB 2241, ... (type strain)
L. johnsonii D3, DSM 20553, JCM 2122, LMG 18184
L. johnsonii MIII-1B.7-2, DSM 100219
L. johnsonii Omniflora, CIP 103654
L. johnsonii A20, CIP 103781, NCDO 20, VPI 11694
L. johnsonii CIP 103652, ATCC 332, CCUG 44520, ...
L. johnsonii 63E, 55 098 301, CIP 103782, CIP ...
L. johnsonii CCUG 31413, JCM 8793, LMG 18206
L. johnsonii WCA-380-WT-3D, DSM 106897
L. johnsonii Cla-CZ-4, DSM 108981
L. johnsonii JCM 1022, KCTC 3141, LMG 18175
L. johnsonii JCM 5812, BCRC 14037, LMG 18193
L. johnsonii JCM 8791, LMG 18204
L. johnsonii JCM 8792, LMG 18205

Section

Lactobacillus johnsonii CCUG 44519 is an anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

Gram-positive
rod-shaped
anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus johnsonii**
	Full Scientific Name (LPSN)	Lactobacillus johnsonii Fujisawa et al. 1992

Information on morphological and physiological properties Morphology

[Ref.: #35586]	Gram stain	positive

[Ref.: #35586]	Cell shape	rod-shaped

[Ref.: #35586]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #35586]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #35586]

Culture medium growth

[Ref.: #35586]

Culture medium composition

expand / minimize

[Ref.: #35586]

Culture medium

CIP Medium 40

[Ref.: #35586]

Culture medium growth

[Ref.: #35586]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #35586]		growth	37 °C
[Ref.: #35586]		growth	37-45 °C
[Ref.: #35586]	no	growth	15 °C
[Ref.: #56362]		growth	30-37 °C
[Ref.: #67770]		growth	37 °C

[Ref.: #35586]	Temperature range	mesophilic
[Ref.: #35586]	Temperature range	psychrophilic
[Ref.: #56362]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56362]	Oxygen tolerance	anaerobe
[Ref.: #56362]	Oxygen tolerance	microaerophile
[Ref.: #35586]	Oxygen tolerance	facultative anaerobe

[Ref.: #67770]

Observation

Assay of Deoxyribonucleic acid

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	arginine ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68381]	hippurate ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68381]	lactose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #35586]	nitrate ChEBI	-	reduction
[Ref.: #35586]	nitrate ChEBI	+	respiration
[Ref.: #35586]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid, API rID32STR
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #35586]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #35586]		acetoin ChEBI	+
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #35586]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR, API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR, API zym
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR, API zym
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR, API zym
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #35586]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #35586]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #35586]	9350	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+/-	-	-	+	-	+/-	+	+	-	+	+	-	-	+/-	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #56362]	4918	-	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #35586]	7614	-	+	+	-	-	+	-	+	-	-	+	+	-	+	-	+	+	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #35586]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lactobacillus johnsonii ATCC 11506	651716998	draft	JGI IMG/M	33959 tax ID	*

[Ref.: #67770]

GC-content

32.5 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #35586]

Culture collection no.

CCUG 44519, IFO 13952, LMG 9437, JCM 8794, CIP 103653, ATCC 11506, NCIMB 8795, NCIMB 8892, JCM 1101, BCRC 14004, BUCSAV 419, CCM 2935, CECT 289, NBRC 13952, VPI 11088

[Ref.: #93443]

SI-ID 92273

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	The aggregation-promoting factor in Lactobacillus delbrueckii ssp. bulgaricus: confirmation of the presence and expression of the apf gene and in silico analysis of the corresponding protein.	Yungareva T, Urshev Z	World J Microbiol Biotechnol	10.1007/s11274-018-2480-1	2018	*
Metabolism	Enhanced phagocytosis of Aggregatibacter actinomycetemcomitans cells by macrophages activated by a probiotic Lactobacillus strain.	Jaffar N, Okinaga T, Nishihara T, Maeda T	J Dairy Sci	10.3168/jds.2017-14355	2018	*
Enzymology	The first alpha-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31.	Kang MS, Okuyama M, Mori H, Kimura A	Biochimie	10.1016/j.biochi.2009.07.018	2009	*
Pathogenicity	Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method.	Callewaert R, Holo H, Devreese B, Van Beeumen J, Nes I, De Vuyst L	Microbiology (Reading)	10.1099/00221287-145-9-2559	1999	*
Metabolism	The groESL chaperone operon of Lactobacillus johnsonii.	Walker DC, Girgis HS, Klaenhammer TR	Appl Environ Microbiol	10.1128/AEM.65.7.3033-3041.1999	1999	*
Metabolism	Utilization of the leucocin A export system in Leuconostoc gelidum for production of a Lactobacillus bacteriocin.	Allison GE, Ahn C, Stiles ME, Klaenhammer TR	FEMS Microbiol Lett	10.1016/0378-1097(95)00241-v	1995	*
Enzymology	Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17.	Allison GE, Worobo RW, Stiles ME, Klaenhammer TR	Appl Environ Microbiol	10.1128/aem.61.4.1371-1377.1995	1995	*
Genetics	Expansion of bacteriocin activity and host range upon complementation of two peptides encoded within the lactacin F operon.	Allison GE, Fremaux C, Klaenhammer TR	J Bacteriol	10.1128/jb.176.8.2235-2241.1994	1994	*
Metabolism	Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane.	Abee T, Klaenhammer TR, Letellier L	Appl Environ Microbiol	10.1128/aem.60.3.1006-1013.1994	1994	*
Enzymology	Molecular analysis of the lactacin F operon.	Fremaux C, Ahn C, Klaenhammer TR	Appl Environ Microbiol	10.1128/aem.59.11.3906-3915.1993	1993	*
Enzymology	Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker.	Allison GE, Klaenhammer TR	Appl Environ Microbiol	10.1128/aem.62.12.4450-4460.1996	1996	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35586 Collection of Institut Pasteur ; Curators of the CIP; CIP 103653
#56362 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 44519

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68381

Automatically annotated from API rID32STR .

#68382

Automatically annotated from API zym .

#93443

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92273.1 )

* These data were automatically processed and therefore are not curated

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