Strain identifier

BacDive ID: 136238

Type strain: No

Species: Lactobacillus johnsonii

Strain history: CIP <- 1993, T. Fujisawa, Public Hlth. Lab., Yokohama, Japan <- T. Mitsuoka, Riken, Japan

NCBI tax ID(s): 33959 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.3 (current version):
version 9.2:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 9.3 (current version)

General

@ref: 35586

BacDive-ID: 136238

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Lactobacillus johnsonii CCUG 44519 is an anaerobe, mesophilic, Gram-positive bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 33959
  • Matching level: species

strain history

@refhistory
67770T. Mitsuoka S8-36 <-- ATCC 11506 <-- A. Snog-Kjaer (Lactobacillus acidophilus) <-- E. Hoff-Jorgensen R-26.
67770T. Fujisawa <-- T. Mitsuoka <-- ATCC 11506 <-- A. Snog-Kjaer <-- E. Hoff-Jorgensen R-26.
35586CIP <- 1993, T. Fujisawa, Public Hlth. Lab., Yokohama, Japan <- T. Mitsuoka, Riken, Japan

doi: 10.13145/bacdive136238.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus johnsonii
  • full scientific name: Lactobacillus johnsonii Fujisawa et al. 1992

@ref: 35586

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus johnsonii

type strain: no

Morphology

cell morphology

  • @ref: 35586
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
35586MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
35586CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
35586positivegrowth37
56362positivegrowth30-37
67770positivegrowth37
35586positivegrowth37-45
35586nogrowth15

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
56362anaerobe
56362microaerophile
35586facultative anaerobe
125439facultative anaerobe100

spore formation

  • @ref: 125439
  • spore formation: yes
  • confidence: 98.7

observation

  • @ref: 67770
  • observation: Assay of Deoxyribonucleic acid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
6838116443D-tagatose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
683816731melezitose-builds acid from
6838128053melibiose-builds acid from
6838117306maltose+builds acid from
6838127941pullulan-builds acid from
68381606565hippurate-hydrolysis
6838140585alpha-cyclodextrin-builds acid from
6838118333D-arabitol-builds acid from
6838130849L-arabinose-builds acid from
6838117992sucrose+builds acid from
6838116634raffinose-builds acid from
6838127082trehalose+builds acid from
6838117716lactose-builds acid from
6838130911sorbitol-builds acid from
6838116899D-mannitol-builds acid from
6838116988D-ribose-builds acid from
6838129016arginine-hydrolysis
3558617632nitrate-reduction
3558616301nitrite-reduction
3558617632nitrate+respiration
68371Potassium 5-ketogluconate-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837112936D-galactose-builds acid from
6838128087glycogen-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

antibiotic resistance

  • @ref: 35586
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
3558615688acetoin+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
35586alcohol dehydrogenase-1.1.1.1
35586lysine decarboxylase-4.1.1.18
35586ornithine decarboxylase-4.1.1.17
68381pyrrolidonyl arylamidase-3.4.19.3

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
35586-++--+-+--++-+-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
35586----------+++--------+/---+-+/-++-++--+/-+/---+/-----------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
56362-++-------+-+---+++------+------

Safety information

risk assessment

  • @ref: 35586
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
124043Lactobacillus johnsonii gene for 16S rRNA, partial sequence, strain: JCM 1101.AB289174656nuccore33959
124043Lactobacillus johnsonii gene for 16S rRNA, partial sequence, strain: JCM 8794.AB289181662nuccore33959
124043Lactobacillus johnsonii gene for 16S rRNA, partial sequence, strain: NBRC 13952.AB6805301498nuccore33959

Genome sequences

  • @ref: 66792
  • description: Lactobacillus johnsonii ATCC 11506
  • accession: 651716998
  • assembly level: draft
  • database: img
  • NCBI tax ID: 33959

GC content

  • @ref: 67770
  • GC-content: 32.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidence
125439BacteriaNetspore_formationAbility to form endo- or exosporesyes98.7
125439BacteriaNetmotilityAbility to perform movementyes87.7
125439BacteriaNetgram_stainReaction to gram-stainingnegative73
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthfacultative anaerobe100

External links

@ref: 35586

culture collection no.: CCUG 44519, IFO 13952, LMG 9437, JCM 8794, CIP 103653, ATCC 11506, NCIMB 8795, NCIMB 8892, JCM 1101, BCRC 14004, BUCSAV 419, CCM 2935, CECT 289, NBRC 13952, VPI 11088

straininfo link

  • @ref: 93443
  • straininfo: 92273

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism7557315Utilization of the leucocin A export system in Leuconostoc gelidum for production of a Lactobacillus bacteriocin.Allison GE, Ahn C, Stiles ME, Klaenhammer TRFEMS Microbiol Lett10.1016/0378-1097(95)00241-v1995Amino Acid Sequence, Bacteriocins/*biosynthesis/*metabolism, Cloning, Molecular, DNA, Bacterial/analysis, Enterococcus/drug effects, Gene Expression/physiology, Lactobacillus/*metabolism, Leuconostoc/genetics/*metabolism, Molecular Sequence Data, Phenotype, PlasmidsEnzymology
Enzymology7747957Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17.Allison GE, Worobo RW, Stiles ME, Klaenhammer TRAppl Environ Microbiol10.1128/aem.61.4.1371-1377.19951995Amino Acid Sequence, Bacteriocins/biosynthesis/*genetics/pharmacology, Cloning, Molecular, Enterococcus faecalis/drug effects, Food Microbiology, Food Preservation, Gene Expression, Genes, Bacterial, Gram-Positive Asporogenous Rods/*genetics/metabolism, Lactobacillus/drug effects, Molecular Sequence Data, Operon, Protein Sorting Signals/genetics, Transformation, GeneticPathogenicity
Genetics8157592Expansion of bacteriocin activity and host range upon complementation of two peptides encoded within the lactacin F operon.Allison GE, Fremaux C, Klaenhammer TRJ Bacteriol10.1128/jb.176.8.2235-2241.19941994Amino Acid Sequence, Bacteriocins/*biosynthesis/chemistry/*genetics, Base Sequence, Genes, Bacterial/*physiology, Genetic Complementation Test, Lactobacillus/genetics/*metabolism, Molecular Sequence Data, Mutation/genetics/physiology, Operon/*physiologyPathogenicity
Metabolism8161167Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane.Abee T, Klaenhammer TR, Letellier LAppl Environ Microbiol10.1128/aem.60.3.1006-1013.19941994Adenosine Triphosphate/metabolism, Bacteriocins/*pharmacology, Cell Membrane/drug effects/metabolism, Detergents/pharmacology, Enterococcus faecalis/drug effects/*metabolism, Hydrogen-Ion Concentration, Lactobacillus/drug effects/*metabolism, Membrane Potentials, Potassium/*metabolism, TemperaturePathogenicity
Enzymology8285694Molecular analysis of the lactacin F operon.Fremaux C, Ahn C, Klaenhammer TRAppl Environ Microbiol10.1128/aem.59.11.3906-3915.19931993Amino Acid Sequence, Bacteriocins/*genetics, Base Sequence, Cloning, Molecular, DNA, Bacterial/genetics, Escherichia coli/genetics, Gene Expression, *Genes, Bacterial, Lactobacillus/*genetics, Molecular Sequence Data, Mutagenesis, Site-Directed, Open Reading Frames, *Operon, Sequence Homology, Amino AcidGenetics
Enzymology8953716Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker.Allison GE, Klaenhammer TRAppl Environ Microbiol10.1128/aem.62.12.4450-4460.19961996Amino Acid Sequence, Bacteriocins/biosynthesis/*genetics, Cloning, Molecular, *Genetic Markers, Lactobacillus/*genetics/immunology, Molecular Sequence Data, Mutation, Open Reading Frames, Transformation, BacterialGenetics
Metabolism10388700The groESL chaperone operon of Lactobacillus johnsonii.Walker DC, Girgis HS, Klaenhammer TRAppl Environ Microbiol10.1128/AEM.65.7.3033-3041.19991999Amino Acid Sequence, Bacterial Proteins/*genetics/metabolism, Base Sequence, Chaperonins/*genetics/metabolism, Cloning, Molecular, DNA, Bacterial, Freezing, Heat-Shock Response, Lactobacillus/*genetics/growth & development/metabolism, Molecular Sequence Data, *Operon, Plasmids/genetics, RNA, Messenger/metabolism, Sequence Analysis, DNA, Transcription, GeneticEnzymology
Pathogenicity10517609Characterization and production of amylovorin L471, a bacteriocin purified from Lactobacillus amylovorus DCE 471 by a novel three-step method.Callewaert R, Holo H, Devreese B, Van Beeumen J, Nes I, De Vuyst LMicrobiology (Reading)10.1099/00221287-145-9-25591999Amino Acid Sequence, *Bacteriocins/biosynthesis/chemistry/isolation & purification/pharmacology, Lactobacillus/drug effects, Lactobacillus acidophilus/drug effects/growth & development/*metabolism, Listeria/drug effects, Mass Spectrometry/methods, Microbial Sensitivity Tests, Molecular Sequence DataGenetics
Enzymology19683032The first alpha-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 31.Kang MS, Okuyama M, Mori H, Kimura ABiochimie10.1016/j.biochi.2009.07.0182009Cloning, Molecular, DNA, Bacterial, Disaccharides/chemistry/metabolism, Escherichia coli/enzymology/genetics, Glycoside Hydrolases/metabolism, Molecular Sequence Data, Potassium Channels, Inwardly Rectifying/genetics/*metabolism, Sequence Alignment, Sequence Homology, Amino Acid, Yeasts/genetics, alpha-GlucosidasesMetabolism
Metabolism29680655Enhanced phagocytosis of Aggregatibacter actinomycetemcomitans cells by macrophages activated by a probiotic Lactobacillus strain.Jaffar N, Okinaga T, Nishihara T, Maeda TJ Dairy Sci10.3168/jds.2017-143552018Aggregatibacter actinomycetemcomitans/*metabolism, Animals, Lactobacillus/*physiology, Macrophages, Mice, *Phagocytosis, *Probiotics
Metabolism29923077The aggregation-promoting factor in Lactobacillus delbrueckii ssp. bulgaricus: confirmation of the presence and expression of the apf gene and in silico analysis of the corresponding protein.Yungareva T, Urshev ZWorld J Microbiol Biotechnol10.1007/s11274-018-2480-12018Bacterial Proteins/*metabolism, Cell Adhesion Molecules/*metabolism, Lactobacillus delbrueckii/*metabolism, Molecular Weight

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
35586Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103653Collection of Institut Pasteur (CIP 103653)
56362Curators of the CCUGhttps://www.ccug.se/strain?id=44519Culture Collection University of Gothenburg (CCUG) (CCUG 44519)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
93443Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID92273.1
124043Isabel Schober, Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG