Lactobacillus johnsonii (DSM 100219, MIII-1B.7-2)

Name: Lactobacillus johnsonii (DSM 100219, MIII-1B.7-2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 131033

Type strain

Strain Designation MIII-1B.7-2

Culture col. no. DSM 100219 MIII-1B.7-2

NCBI tax ID(s) 33959

Special Collections DSMZ Mouse DZIF

History <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany <- M. W. Hornef, RWTH Aachen, Germany; MIII-1B.7-2

Links

Lactobacillus johnsonii MIII-1B.7-2 is a microaerophile bacterium that was isolated from newborn SPF mouse .

microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 400606

DSM 100219

@ref 20215

Last LPSN update 2026-02-09 11:31:22

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Lactobacillus

Species Lactobacillus johnsonii

Full scientific name Lactobacillus johnsonii Fujisawa et al. 1992

BacDive ID	Other strains from **Lactobacillus johnsonii** (14)	Type strain
6560	L. johnsonii DSM 10533, ATCC 33200, NCFB 2241, CCUG 30725, ... (type strain)
6561	L. johnsonii D3, DSM 20553, JCM 2122, LMG 18184
135326	L. johnsonii Omniflora, CIP 103654
135886	L. johnsonii A20, CIP 103781, NCDO 20, VPI 11694
136237	L. johnsonii CIP 103652, ATCC 332, CCUG 44520, JCM 1017, ...
136238	L. johnsonii CCUG 44519, IFO 13952, LMG 9437, JCM 8794, ...
137649	L. johnsonii 63E, 55 098 301, CIP 103782, CIP 71.34, NCDO ...
146549	L. johnsonii CCUG 31413, JCM 8793, LMG 18206
159210	L. johnsonii WCA-380-WT-3D, DSM 106897
160341	L. johnsonii Cla-CZ-4, DSM 108981
160897	L. johnsonii JCM 1022, KCTC 3141, LMG 18175
165303	L. johnsonii JCM 5812, BCRC 14037, LMG 18193
165960	L. johnsonii JCM 8791, LMG 18204
165961	L. johnsonii JCM 8792, LMG 18205

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		91.5
125438		93.619	positive

Colony morphology

22325

Incubation period1-2 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22325	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22325	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
22325	microaerophile
125439	microaerophile	95.4

Spore formation

@ref	Spore formation	Confidence
125438		92.7
125439		91.5

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
22325	-	-	-	-	-	-	-	-	-	-	+/-	+	+	+/-	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	+	-	+	+/-	-	-	-	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Muridae (Mouse/Rat)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22325	newborn SPF mouse (6-day-old)	Freiburg	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence KU196088 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	5652
Soil Samples	4782
Animal Samples	173268
Plant Samples	1535
Total Samples	185237

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
22325	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2462248v1 assembly for Lactobacillus johnsonii DSM 100219	contig	GCA_024622485	33959.606	33959	72.83

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22325	Lactobacillus johnsonii strain DSM 100219 16S ribosomal RNA gene, partial sequence	KU196088	1473		33959

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactobacillus johnsonii DSM 100219
NCBI: GCA_024622485

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	91.50	no
125439	motility	BacteriaNetⓘ	no	81.30	no
125439	gram_stain	BacteriaNetⓘ	positive	87.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	95.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus johnsonii DSM 100219
NCBI: GCA_024622485.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.62	no
125438	anaerobic	anaerobicⓘ	no	74.68	yes
125438	aerobic	aerobicⓘ	no	97.00	no
125438	spore-forming	spore-formingⓘ	no	92.70	no
125438	thermophilic	thermophileⓘ	no	97.00	no
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Standardized Natural Citrus Extract dietary supplementation influences sows' microbiota, welfare, and preweaning piglets' performances in commercial rearing conditions.	Cisse S, Benarbia MEA, Burel A, Friedrich M, Gabinaud B, Belz E, Guilet D, Chicoteau P, Zemb O.	Transl Anim Sci	10.1093/tas/txaa059	2020
Metabolism	Bile acids drive the newborn's gut microbiota maturation.	van Best N, Rolle-Kampczyk U, Schaap FG, Basic M, Olde Damink SWM, Bleich A, Savelkoul PHM, von Bergen M, Penders J, Hornef MW.	Nat Commun	10.1038/s41467-020-17183-8	2020

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22325	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100219
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactobacillus johnsonii (DSM 100219, MIII-1B.7-2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024622485

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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