Name: Lactobacillus johnsonii DSM 10533
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6560

Type strain:

Species: Lactobacillus johnsonii

Culture col. no.: DSM 10533, ATCC 33200, NCFB 2241, CCUG 30725, JCM 2012, KCTC 3801, VPI 7960, CCM 4384, CGMCC 1.3348, CGMCC 1.3397, CIP 103620, KCTC 5085

Strain history: <- CCUG <- JCM <- ATCC 33200 <- VPI 7960 <- DW Lambe, Jr.

NCBI tax ID(s): 525330 (strain), 33959 (species)

Other strains from Lactobacillus johnsonii

L. johnsonii D3, DSM 20553, JCM 2122, LMG 18184
L. johnsonii MIII-1B.7-2, DSM 100219
L. johnsonii CIP 103654
L. johnsonii CIP 103781, NCDO 20
L. johnsonii CIP 103652, ATCC 332, CCUG 44520, ...
L. johnsonii CCUG 44519, IFO 13952, LMG 9437, ...
L. johnsonii CIP 103782, CIP 71.34, NCDO 3
L. johnsonii CCUG 31413, JCM 8793, LMG 18206
L. johnsonii WCA-380-WT-3D, DSM 106897
L. johnsonii Cla-CZ-4, DSM 108981
L. johnsonii JCM 1022, KCTC 3141, LMG 18175
L. johnsonii JCM 5812, BCRC 14037, LMG 18193
L. johnsonii JCM 8791, LMG 18204
L. johnsonii JCM 8792, LMG 18205

Section

Lactobacillus johnsonii DSM 10533 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human blood.

Gram-positive
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus johnsonii**
	Full Scientific Name (PNU)	Lactobacillus johnsonii Fujisawa et al. 1992

Information on morphological and physiological properties Morphology

[Ref.: #67771]

Gram stain

positive

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3968]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #3968]

Culture medium growth

[Ref.: #3968]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #3968]

Culture medium composition

expand / minimize

[Ref.: #34023]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #34023]

Culture medium growth

[Ref.: #34023]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #3968]	growth	37 °C
[Ref.: #34023]	growth	37 °C
[Ref.: #50246]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #3968]	Temperature range	mesophilic
[Ref.: #34023]	Temperature range	mesophilic
[Ref.: #50246]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50246]	Oxygen tolerance	anaerobe
[Ref.: #67771]	Oxygen tolerance	anaerobe

[Ref.: #3968]	Murein short key	A11.31
[Ref.: #3968]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #3968]	7936	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	+	-	+	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-
[Ref.: #3968]	7935	-	-	-	-	-	-	-	-	-	-	+/-	+	+	+/-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	+	-	+	-	-	-	+	+	-	-	+	-	-	+/-	-	-	-	-	-	-	-
[Ref.: #3968]	7934	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #3968]	7933	-	-	-	-	-	-	-	-	-	-	+/-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	+	-	-	-	+	+/-	-	-	+/-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3968]	Sample type/isolated from	human blood
[Ref.: #3968]	Country	Belgium
[Ref.: #3968]	Country ISO 3 Code	BEL
[Ref.: #3968]	Continent	Europe

[Ref.: #67771]	Sample type/isolated from	From human blood
[Ref.: #67771]	Country	Belgium
[Ref.: #67771]	Country ISO 3 Code	BEL
[Ref.: #67771]	Continent	Europe

[Ref.: #50246]	Sample type/isolated from	Human blood

[Ref.: #67770]	Sample type/isolated from	Human blood
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Fluids	#Blood

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC071811 (>99% sequence identity) for Lactobacillus from Microbeatlas

Aquatic Samples	5652
Soil Samples	4782
Animal Samples	173268
Plant Samples	1535
Total Samples	185237

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3968]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Lactobacillus johnsonii 16S-23S rRNA intergenic spacer region	AF074860	215	ENA	33959 tax ID	*
[Ref.: #20218]	Lactobacillus johnsonii 16S rRNA gene	AJ002515	1487	ENA	33959 tax ID	*
[Ref.: #20218]	Lactobacillus johnsonii partial 16S rRNA gene, type strain CIP 103620T	HE573915	1543	ENA	33959 tax ID	*
[Ref.: #20218]	Lactobacillus johnsonii strain DSM 10533 16S ribosomal RNA gene, partial sequence	EF468091	411	ENA	33959 tax ID	*
[Ref.: #20218]	Lactobacillus johnsonii gene for 16S rRNA, partial sequence, strain: JCM 2012	AB289175	658	ENA	33959 tax ID	*
[Ref.: #67771]	Lactobacillus johnsonii gene for 16S ribosomal RNA, partial sequence, strain: JCM 2012	LC071811	1470	ENA	33959 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Lactobacillus johnsonii ATCC 33200	525330.7	wgs	PATRIC	525330 tax ID	*
[Ref.: #66792]	Lactobacillus johnsonii ATCC 33200	525330.3	wgs	PATRIC	525330 tax ID	*
[Ref.: #66792]	Lactobacillus johnsonii ATCC 33200	2657245008	draft	JGI IMG/M	525330 tax ID	*
[Ref.: #66792]	Lactobacillus johnsonii ATCC 33200	643886046	draft	JGI IMG/M	525330 tax ID	*
[Ref.: #67770]	Lactobacillus johnsonii ATCC 33200 ATCC 33200	GCA_000159355	scaffold	GenBank	525330 tax ID
[Ref.: #67771]	Lactobacillus johnsonii ATCC 33200 ATCC 33200	GCA_001433975	contig	GenBank	525330 tax ID

[Ref.: #3968]	GC-content	33.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	34.4 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	33 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #3968]

Culture collection no.

DSM 10533, ATCC 33200, NCFB 2241, CCUG 30725, JCM 2012, KCTC 3801, VPI 7960, CCM 4384, CGMCC 1.3348, CGMCC 1.3397, CIP 103620, KCTC 5085

[Ref.: #20218]

Associated Passport(s) in StrainInfo

13039, 13036, 13041

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Taxonomic study of the Lactobacillus acidophilus group, with recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and synonymy of Lactobacillus acidophilus group A3 (Johnson et al. 1980) with the type strain of Lactobacillus amylovorus (Nakamura 1981).	Fujisawa T, Benno Y, Yaeshima T, Mitsuoka T	Int J Syst Bacteriol	10.1099/00207713-42-3-487	1992	*
Enzymology	Functional analysis of the gene encoding immunity to lactacin F, lafI, and its use as a Lactobacillus-specific, food-grade genetic marker.	Allison GE, Klaenhammer TR	Appl Environ Microbiol	10.1128/aem.62.12.4450-4460.1996	1996	*
Genetics	Identification of genes associated with the long-gut-persistence phenotype of the probiotic Lactobacillus johnsonii strain NCC533 using a combination of genomics and transcriptome analysis.	Denou E, Pridmore RD, Berger B, Panoff JM, Arigoni F, Brussow H	J Bacteriol	10.1128/JB.01637-07	2008	*
Phylogeny	Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods.	Sarmiento-Rubiano LA, Berger B, Moine D, Zuniga M, Perez-Martinez G, Yebra MJ	BMC Genomics	10.1186/1471-2164-11-504	2010	*
Phylogeny	Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents.	Killer J, Havlik J, Vlkova E, Rada V, Pechar R, Benada O, Kopecny J, Kofronova O, Sechovcova H	Int J Syst Evol Microbiol	10.1099/ijs.0.054924-0	2014	*
Enzymology	Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture.	Starke IC, Zentek J, Vahjen W	Benef Microbes	10.3920/BM2014.0052	2015	*
Metabolism	A highly acid-resistant novel strain of Lactobacillus johnsonii No. 1088 has antibacterial activity, including that against Helicobacter pylori, and inhibits gastrin-mediated acid production in mice.	Aiba Y, Nakano Y, Koga Y, Takahashi K, Komatsu Y	Microbiologyopen	10.1002/mbo3.252	2015	*
Pathogenicity	Extending viability of Lactobacillus plantarum and Lactobacillus johnsonii by microencapsulation in alginate microgels.	Tiani KA, Yeung TW, McClements DJ, Sela DA	Int J Food Sci Nutr	10.1080/09637486.2017.1343285	2017	*

References

#3968

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10533

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34023 ; Curators of the CIP;
#50246 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30725

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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