Name: Actinoalloteichus spitiensis RMV-1378
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13193

Type strain:

Species: Actinoalloteichus spitiensis

Strain Designation: RMV-1378

Culture col. no.: DSM 44848, JCM 12472, MTCC 6194, BCRC 16823, CBS 11706, CIP 109023, NBRC 102582, NCIMB 14384

Strain history: S. Mayilraj RMV-1378.

NCBI tax ID(s): 1114962 (strain), 252394 (species)

SI-ID 281439

DSM 44848, JCM 12472, CIP 109023, BCRC 16823, CCRC 16823, NBRC 102582

Special Collections

Section

Actinoalloteichus spitiensis RMV-1378 is an aerobe, Gram-positive bacterium that was isolated from soil.

Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinoalloteichus*
	Species	**Actinoalloteichus spitiensis**
	Full Scientific Name (LPSN)	Actinoalloteichus spitiensis Singla et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31514]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %90.097

[Ref.: #69480]	Motility	no Confidence in %95

[Ref.: #19882]	Colony color	Beige
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 2

[Ref.: #19882]	Colony color	Ivory
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 3

[Ref.: #19882]	Colony color	Beige
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 4

[Ref.: #19882]	Colony color	Ivory
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 5

[Ref.: #19882]	Colony color	Beige
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 6

[Ref.: #19882]	Colony color	Ivory
[Ref.: #19882]	Incubation period	10-14 days
[Ref.: #19882]	Cultivation medium used	ISP 7

[Ref.: #31514]

Pigment production

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 2

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 3

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 4

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 5

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 6

[Ref.: #19882]	Multicellular complex forming ability	no
[Ref.: #19882]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12029]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #12029]

Culture medium growth

[Ref.: #12029]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12029]

Culture medium composition

expand / minimize

[Ref.: #12029]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12029]

Culture medium growth

[Ref.: #12029]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12029]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 7

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 6

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 5

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 4

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 3

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

[Ref.: #19882]

Culture medium

ISP 2

[Ref.: #19882]

Culture medium growth

[Ref.: #19882]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #12029]	growth	30 °C
[Ref.: #19882]	optimum	28 °C
[Ref.: #31514]	growth	20-37 °C
[Ref.: #31514]	optimum	25 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #31514]			optimum	8
[Ref.: #31514]			growth	06-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31514]

Oxygen tolerance

aerobe

[Ref.: #31514]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31514]		NaCl		growth	<2	%

[Ref.: #67770]

Observation

quinones: MK-9(H₄), MK-10(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31514]	citrate ChEBI	+	carbon source
[Ref.: #31514]	galactose ChEBI	+	carbon source
[Ref.: #31514]	glucose ChEBI	+	carbon source
[Ref.: #31514]	mannitol ChEBI	+	carbon source
[Ref.: #31514]	mannose ChEBI	+	carbon source
[Ref.: #31514]	raffinose ChEBI	+	carbon source
[Ref.: #31514]	rhamnose ChEBI	+	carbon source
[Ref.: #31514]	ribose ChEBI	+	carbon source
[Ref.: #31514]	salicin ChEBI	+	carbon source
[Ref.: #31514]	succinate ChEBI	+	carbon source
[Ref.: #31514]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19882]	694	not determinedn.d.	+	+	+	+	+	+	-	+	-	+	+	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12029]	Sample type/isolated from	soil
[Ref.: #12029]	Geographic location (country and/or sea, region)	cold desert of the Himalayas
[Ref.: #12029]	Country	India
[Ref.: #12029]	Country ISO 3 Code	IND
[Ref.: #12029]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil from the Lahaul-Spiti Valley, cold desert
[Ref.: #67770]	Geographic location (country and/or sea, region)	Himalaya
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY426714 (>99% sequence identity) for Actinoalloteichus from Microbeatlas

Aquatic Samples	125
Soil Samples	1111
Animal Samples	269
Plant Samples	164
Total Samples	1669

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12029]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19882]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12029]	Actinoalloteichus spitiensis strain MTCC 6194 16S ribosomal RNA gene, partial sequence	AY426714	1476	GenBank ENA	1114962 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinoalloteichus spitiensis RMV-1378 RMV-1378	GCA_000239155	contig	GenBank ENA	1114962 tax ID
[Ref.: #66792]	Actinoalloteichus spitiensis RMV-1378	1114962.3	wgs	PATRIC	1114962 tax ID	*
[Ref.: #66792]	Actinoalloteichus spitiensis RMV-1378	2563366745	draft	JGI IMG/M	1114962 tax ID	*

[Ref.: #31514]	GC-content	72 mol%
[Ref.: #67770]	GC-content	72 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinoalloteichus spitiensis RMV-1378 PATRIC: 1114962.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.097	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.367	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	54.213	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	87.002	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.654	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	95	no

Availability in culture collections External links

[Ref.: #12029]

Culture collection no.

DSM 44848, JCM 12472, MTCC 6194, BCRC 16823, CBS 11706, CIP 109023, NBRC 102582, NCIMB 14384

[Ref.: #82401]

SI-ID 281439

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas.	Singla AK, Mayilraj S, Kudo T, Krishnamurthi S, Prasad GS, Vohra RM	Int J Syst Evol Microbiol	10.1099/ijs.0.63720-0	2005	*

References

#12029

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44848

#19882

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31514

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27810 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82401

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID281439.1 )

#27810

IJSEM 2561 2005 ( DOI 10.1099/ijs.0.63720-0 , PubMed 16280527 )

* These data were automatically processed and therefore are not curated

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