Actinoalloteichus spitiensis (DSM 44848, JCM 12472, MTCC 6194)

Name: Actinoalloteichus spitiensis (DSM 44848, JCM 12472, MTCC 6194)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13193

Type strain

Strain Designation RMV-1378

Culture col. no. DSM 44848 JCM 12472 MTCC 6194 RMV-1378 NCIMB 14384 4 more

NCBI tax ID(s) 1114962 252394

Special Collections DSMZ JCM CIP Wink compendium Literature strains

History <- MTCC <- A. K. Singla S. Mayilraj RMV-1378.

Links

Actinoalloteichus spitiensis RMV-1378 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from soil.

Gram-positive aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 281439

DSM 44848,JCM 12472,CIP 109023,BCRC 16823,CCRC 16823,NBRC 102582

@ref 20215

Last LPSN update 2025-12-16 09:50:02

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinoalloteichus

Species Actinoalloteichus spitiensis

Full scientific name Actinoalloteichus spitiensis Singla et al. 2005

Morphology

Cell morphology

@ref	Confidence	Gram stain
31514		positive
125438	95
125438	90.097	positive
125439	100	positive
125439	94.3

Colony morphology

@ref	Colony color	Incubation period	Medium used
19882	Beige	10-14 days	ISP 2
19882	Ivory	10-14 days	ISP 3
19882	Beige	10-14 days	ISP 4
19882	Ivory	10-14 days	ISP 5
19882	Beige	10-14 days	ISP 6
19882	Ivory	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19882		ISP 2
19882		ISP 3
19882		ISP 4
19882		ISP 5
19882		ISP 6
19882		ISP 7

Pigmentation

31514

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12029	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12029	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19882	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
19882	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19882	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19882	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19882	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19882	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
12029	positive	growth	30	mesophilic
19882	positive	optimum	28	mesophilic
31514	positive	growth	20-37
31514	positive	optimum	25	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
31514	positive	optimum	8
31514	positive	growth	06-11	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31514	aerobe
125439	obligate aerobe	98.8

Spore formation

@ref	Spore formation	Confidence
31514
125439		95.3

Halophily

@ref	Salt	Growth	Tested relation	Concentration
31514	NaCl	positive	growth	<2 %

Observation

67770

Observationquinones: MK-9(H₄), MK-10(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31514	16947 ChEBI	citrate	+	carbon source
31514	28260 ChEBI	galactose	+	carbon source
31514	17234 ChEBI	glucose	+	carbon source
31514	29864 ChEBI	mannitol	+	carbon source
31514	37684 ChEBI	mannose	+	carbon source
31514	16634 ChEBI	raffinose	+	carbon source
31514	26546 ChEBI	rhamnose	+	carbon source
31514	33942 ChEBI	ribose	+	carbon source
31514	17814 ChEBI	salicin	+	carbon source
31514	30031 ChEBI	succinate	+	carbon source
31514	17992 ChEBI	sucrose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	+	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19882	not determinedn.d.	+	+	+	+	+	+	-	+	-	+	+	+	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12029	soil	cold desert of the Himalayas	India	IND	Asia
67770	Soil from the Lahaul-Spiti Valley, cold desert	Himalaya	India	IND	Asia

Taxonmaps

69479

Global distribution of 16S sequence AY426714 (>99% sequence identity) for Actinoalloteichus from Microbeatlas

Aquatic Samples	125
Soil Samples	1111
Animal Samples	269
Plant Samples	164
Total Samples	1669

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12029	1	Risk group (German classification) According to TRBA 466
19882	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
67770	ASM23915v2 assembly for Actinoalloteichus spitiensis RMV-1378	contig	GCA_000239155	1114962.9	2563366745	1114962
66792	Actinoalloteichus spitiensis RMV-1378	contig		1114962.3		1114962

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12029	Actinoalloteichus spitiensis strain MTCC 6194 16S ribosomal RNA gene, partial sequence	AY426714	1476		1114962

GC content

@ref	GC-content (mol%)	Method
31514	72
67770	72	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinoalloteichus spitiensis RMV-1378 RMV-1378
NCBI: GCA_000239155

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	95.30	no
125439	motility	BacteriaNetⓘ	no	94.30	no
125439	gram_stain	BacteriaNetⓘ	positive	100.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinoalloteichus spitiensis RMV-1378
PATRIC: 1114962.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.10	yes
125438	anaerobic	anaerobicⓘ	no	94.37	yes
125438	spore-forming	spore-formingⓘ	yes	54.21	no
125438	aerobic	aerobicⓘ	yes	87.00	no
125438	thermophilic	thermophileⓘ	no	93.65	yes
125438	flagellated	motile2+ⓘ	no	95.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Complete genome sequence of the actinomycete Actinoalloteichus hymeniacidonis type strain HPA 177^T isolated from a marine sponge.	Schaffert L, Albersmeier A, Winkler A, Kalinowski J, Zotchev SB, Ruckert C.	Stand Genomic Sci	10.1186/s40793-016-0213-3	2016
Phylogeny	Actinoalloteichus fjordicus sp. nov. isolated from marine sponges: phenotypic, chemotaxonomic and genomic characterisation.	Nouioui I, Ruckert C, Willemse J, van Wezel GP, Klenk HP, Busche T, Kalinowski J, Bredholt H, Zotchev SB.	Antonie Van Leeuwenhoek	10.1007/s10482-017-0920-9	2017
Phylogeny	Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas.	Singla AK, Mayilraj S, Kudo T, Krishnamurthi S, Prasad GS, Vohra RM	Int J Syst Evol Microbiol	10.1099/ijs.0.63720-0	2005

References

#12029	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44848
#19882	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27810	IJSEM 2561 2005 ( DOI 10.1099/ijs.0.63720-0 , PubMed 16280527 )
#31514	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27810
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actinoalloteichus spitiensis (DSM 44848, JCM 12472, MTCC 6194)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000239155

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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