Strain identifier

BacDive ID: 13193

Type strain: Yes

Species: Actinoalloteichus spitiensis

Strain Designation: RMV-1378

Strain history: S. Mayilraj RMV-1378.

NCBI tax ID(s): 1114962 (strain), 252394 (species)

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General

@ref: 12029

BacDive-ID: 13193

DSM-Number: 44848

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Actinoalloteichus spitiensis RMV-1378 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
252394species
1114962strain

strain history

@refhistory
12029<- MTCC <- A. K. Singla
67770S. Mayilraj RMV-1378.

doi: 10.13145/bacdive13193.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Actinoalloteichus
  • species: Actinoalloteichus spitiensis
  • full scientific name: Actinoalloteichus spitiensis Singla et al. 2005

@ref: 12029

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Actinoalloteichus

species: Actinoalloteichus spitiensis

full scientific name: Actinoalloteichus spitiensis Singla et al. 2005

strain designation: RMV-1378

type strain: yes

Morphology

cell morphology

  • @ref: 31514
  • gram stain: positive

colony morphology

@refcolony colorincubation periodmedium used
19882Beige10-14 daysISP 2
19882Ivory10-14 daysISP 3
19882Beige10-14 daysISP 4
19882Ivory10-14 daysISP 5
19882Beige10-14 daysISP 6
19882Ivory10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19882noISP 2
19882noISP 3
19882noISP 4
19882noISP 5
19882noISP 6
19882noISP 7

pigmentation

  • @ref: 31514
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12029TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12029GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19882ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
19882ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19882ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19882ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19882ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19882ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
12029positivegrowth30mesophilic
19882positiveoptimum28mesophilic
31514positivegrowth20-37
31514positiveoptimum25mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31514positivegrowth06-11alkaliphile
31514positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 31514
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31514
  • spore formation: no

halophily

  • @ref: 31514
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <2 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4), MK-10(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3151416947citrate+carbon source
3151428260galactose+carbon source
3151417234glucose+carbon source
3151429864mannitol+carbon source
3151437684mannose+carbon source
3151416634raffinose+carbon source
3151426546rhamnose+carbon source
3151433942ribose+carbon source
3151417814salicin+carbon source
3151430031succinate+carbon source
3151417992sucrose+carbon source

enzymes

@refvalueactivityec
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19882++++++-+-++++-+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12029soilcold desert of the HimalayasIndiaINDAsia
67770Soil from the Lahaul-Spiti Valley, cold desertHimalayaIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_5097.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1336;97_3030;98_3805;99_5097&stattab=map
  • Last taxonomy: Actinoalloteichus
  • 16S sequence: AY426714
  • Sequence Identity:
  • Total samples: 1669
  • soil counts: 1111
  • aquatic counts: 125
  • animal counts: 269
  • plant counts: 164

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
120291Risk group (German classification)
198821Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12029
  • description: Actinoalloteichus spitiensis strain MTCC 6194 16S ribosomal RNA gene, partial sequence
  • accession: AY426714
  • length: 1476
  • database: ena
  • NCBI tax ID: 1114962

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinoalloteichus spitiensis RMV-13781114962.3wgspatric1114962
66792Actinoalloteichus spitiensis RMV-13782563366745draftimg1114962
67770Actinoalloteichus spitiensis RMV-1378 RMV-1378GCA_000239155contigncbi1114962

GC content

@refGC-contentmethod
3151472
6777072high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.654no
flagellatedno97.572no
gram-positiveyes90.131no
anaerobicno98.741yes
aerobicyes95.518yes
halophileno55.733no
spore-formingno68.188yes
thermophileno98.78no
glucose-utilyes88.569yes
glucose-fermentno90.78no

External links

@ref: 12029

culture collection no.: DSM 44848, JCM 12472, MTCC 6194, BCRC 16823, CBS 11706, CIP 109023, NBRC 102582, NCIMB 14384

straininfo link

  • @ref: 82401
  • straininfo: 281439

literature

  • topic: Phylogeny
  • Pubmed-ID: 16280527
  • title: Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas.
  • authors: Singla AK, Mayilraj S, Kudo T, Krishnamurthi S, Prasad GS, Vohra RM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63720-0
  • year: 2005
  • mesh: Actinomycetales/classification/genetics/growth & development/*isolation & purification, Cold Temperature, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, *Desert Climate, India, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/analysis/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
12029Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44848)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44848
19882Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44848.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31514Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2781028776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82401Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID281439.1StrainInfo: A central database for resolving microbial strain identifiers