Name: Bacillus spizizenii TU-B-10
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1193

Type strain:

Species: Bacillus spizizenii

Strain Designation: TU-B-10

Culture col. no.: DSM 15029, NRRL B-23049, JCM 12233, CIP 106094, LMG 19156, NBRC 101239

Strain history: CIP <- 1999, L.K. Nakamura, NCAUR, Illinois, USA <- F.M. Cohan: strain TU-B-10

NCBI tax ID(s): 96241 (species)

SI-ID 14146

LMG 19156, NRRL B-23049, CIP 106094, DSM 15029, KCTC 3705, NBRC 101239, BCRC 17366, CCRC 17366, JCM 12233

Special Collections

Other strains from Bacillus spizizenii

Section

Bacillus spizizenii TU-B-10 is an obligate aerobe, spore-forming, Gram-positive human pathogen that was isolated from tunisian desert.

Gram-positive
motile
rod-shaped
spore-forming
human pathogen
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus spizizenii**
	Full Scientific Name (LPSN)	Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

Synonym

Bacillus subtilis subsp. spizizenii

Information on morphological and physiological properties Morphology

[Ref.: #123361]	Gram stain	positive

[Ref.: #123361]	Cell shape	rod-shaped

[Ref.: #123361]	Motility	yes

[Ref.: #123361]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5680]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #5680]

Culture medium growth

[Ref.: #5680]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5680]

Culture medium composition

expand / minimize

[Ref.: #39469]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #39469]

Culture medium growth

[Ref.: #39469]

Culture medium composition

expand / minimize

[Ref.: #123361]

Culture medium

CIP Medium 3

[Ref.: #123361]

Culture medium growth

[Ref.: #123361]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123361]

Culture medium

CIP Medium 316

[Ref.: #123361]

Culture medium growth

[Ref.: #123361]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5680]		growth	30 °C
[Ref.: #39469]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #123361]		growth	22-37 °C
[Ref.: #123361]	no	growth	10 °C
[Ref.: #123361]	no	growth	45 °C
[Ref.: #123361]	no	growth	55 °C

	pH	Kind of pH		pH
[Ref.: #123361]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #123361]

Oxygen tolerance

obligate aerobe

[Ref.: #123361]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #123361]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123361]	citrate ChEBI	-	carbon source
[Ref.: #123361]	esculin ChEBI	+	hydrolysis
[Ref.: #123361]	hippurate ChEBI	-	hydrolysis
[Ref.: #123361]	nitrate ChEBI	+	reduction
[Ref.: #123361]	nitrate ChEBI	-	respiration
[Ref.: #123361]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123361]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123361]		acetoin ChEBI	+
[Ref.: #123361]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123361]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123361]	amylase	+
[Ref.: #123361]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123361]	caseinase	+	3.4.21.50
[Ref.: #123361]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #123361]	DNase	+
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123361]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #123361]	gelatinase	+
[Ref.: #123361]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #123361]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123361]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123361]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123361]	oxidase	+
[Ref.: #123361]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123361]	tween esterase	+
[Ref.: #123361]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123361]	1532	not determinedn.d.	+	+	-	+	+	-	+	+	+	+	+	-	-	-	-	+	+	+	+	+	+	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123361]	9244	-	+	-	+	-	+	-	-	-	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5680]	Sample type/isolated from	tunisian desert
[Ref.: #5680]	Country	Tunisia
[Ref.: #5680]	Country ISO 3 Code	TUN
[Ref.: #5680]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Tunisian desert

[Ref.: #123361]	Sample type/isolated from	Environment, Soil
[Ref.: #123361]	Geographic location (country and/or sea, region)	Sahara desert
[Ref.: #123361]	Country	Tunisia
[Ref.: #123361]	Country ISO 3 Code	TUN
[Ref.: #123361]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Desert

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF074970 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5680]	Pathogenicity (human)	yes, in single cases
[Ref.: #5680]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123361]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #124043]	Bacillus subtilis subsp. spizizenii strain NBRC 101239 16S ribosomal RNA gene, partial sequence.	KR818222	1213	GenBank ENA	96241 tax ID	*
[Ref.: #20218]	Bacillus subtilis subsp. spizizenii strain BCRC 17366 16S ribosomal RNA gene, partial sequence	EF433402	1468	GenBank ENA	96241 tax ID	*
[Ref.: #20218]	Bacillus subtilis subsp. spizizenii gene for 16S rRNA, partial sequence, strain: NBRC 101239	AB325584	1475	GenBank ENA	96241 tax ID	*
[Ref.: #124043]	Bacillus subtilis subsp. spizizenii strain NBRC 101239 16S ribosomal RNA gene, partial sequence.	MN526961	498	GenBank ENA	96241 tax ID	*
[Ref.: #20218]	Bacillus subtilis subsp. spizizenii 16S ribosomal RNA gene, partial sequence	AF074970	1409	GenBank ENA	96241 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus spizizenii TU-B-10	GCA_000227465	complete	GenBank ENA	1052585 tax ID
[Ref.: #66792]	Bacillus spizizenii NBRC 101239	GCA_007989785	contig	GenBank ENA	96241 tax ID	*
[Ref.: #66792]	Bacillus subtilis subsp. spizizenii strain NBRC 101239	96241.37	wgs	PATRIC	96241 tax ID	*
[Ref.: #66792]	Bacillus subtilis spizizenii TU-B-10, DSM 15029	2511231064	complete	JGI IMG/M	1052585 tax ID	*

Availability in culture collections External links

[Ref.: #5680]

Culture collection no.

DSM 15029, NRRL B-23049, JCM 12233, CIP 106094, LMG 19156, NBRC 101239

[Ref.: #70850]

SI-ID 14146

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.	Bhattacharya D, de Los Santos Villalobos S, Ruiz VV, Selvin J, Mukherjee J	Antonie Van Leeuwenhoek	10.1007/s10482-020-01472-9	2020	*

References

#5680

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15029

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39469 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70850

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14146.1 )

#123361 Collection of Institut Pasteur ; Curators of the CIP; CIP 106094

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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