Bacillus spizizenii (DSM 15029, NRRL B-23049, TU-B-10)

Name: Bacillus spizizenii (DSM 15029, NRRL B-23049, TU-B-10)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1193

Type strain

Strain Designation TU-B-10

Culture col. no. DSM 15029 NRRL B-23049 TU-B-10 NBRC 101239 LMG 19156 2 more

NCBI tax ID(s) 96241

Special Collections DSMZ JCM CIP

History DSMZ <- L. K. Nakamura, NRRL JCM <-- NRRL B-23049 <-- F. M. Cohan TU-B-10. CIP <- 1999, L.K. Nakamura, NCAUR, Illinois, USA <- F.M. Cohan: strain TU-B-10

Links

Bacillus spizizenii TU-B-10 is an obligate aerobe, spore-forming, Gram-positive bacterium that was isolated from tunisian desert.

spore-forming Gram-positive motile rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 14146

LMG 19156,NRRL B-23049,CIP 106094,DSM 15029,KCTC 3705,NBRC 101239,BCRC 17366,CCRC 17366,JCM 12233

@ref 20215

Last LPSN update 2026-05-29 08:25:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus spizizenii

Full scientific name Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

Synonyms (1)

Bacillus subtilis subsp. spizizenii

BacDive ID	Other strains from **Bacillus spizizenii** (7)	Type strain
1187	B. spizizenii 231, NRS-231, DSM 347, ATCC 6633, CCM 1999, ...
1188	B. spizizenii DSM 618, CIP 107063
1189	B. spizizenii W 23, DSM 1087
1190	B. spizizenii W23 2A2, 2A2, DSM 6395, BGSC 2A2, WB 672
1191	B. spizizenii SB 623, DSM 6399, BGSC 2A1
1192	B. spizizenii W23 SR, DSM 6405, BGSC 2A3
23702	B. spizizenii W23, DSM 8439, CCM 2268, IAM 12021, JCM 20354, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123361	positive	rod-shaped

Colony morphology

123361

Hemolysis ability1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5680	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39469	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
123361	CIP Medium 3	Medium recipe at CIP
123361	CIP Medium 316	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5680	positive	growth	30
39469	positive	growth	30
67770	positive	growth	30
123361	positive	growth	22-37
123361	negative	growth	10
123361	negative	growth	45
123361	negative	growth	55

Culture pH

@ref	Ability	Type	PH
123361	positive	growth	6

Physiology and metabolism

Oxygen tolerance

123361

Oxygen toleranceobligate aerobe

Spore formation

123361

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123361	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123361	16947 ChEBI	citrate	-	carbon source
123361	4853 ChEBI	esculin	+	hydrolysis
123361	606565 ChEBI	hippurate	-	hydrolysis
123361	17632 ChEBI	nitrate	+	reduction
123361	17632 ChEBI	nitrate	-	respiration
123361	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123361	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123361	15688 ChEBI	acetoin	+
123361	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123361	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123361	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
123361	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123361	caseinase	+	3.4.21.50
123361	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123361	DNase	+
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
123361	gamma-glutamyltransferase	+	2.3.2.2
123361	gelatinase	+
123361	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123361	lipase	-
68382	lipase (C 14)	-		from API zym
123361	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123361	ornithine decarboxylase	-	4.1.1.17
123361	oxidase	+
123361	protease	+
68382	trypsin	-	3.4.21.4	from API zym
123361	tween esterase	+
123361	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123361	-	+	-	+	-	+	-	-	-	-	+	+	-	+	-	+	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123361	not determinedn.d.	+	+	-	+	+	-	+	+	+	+	+	-	-	-	-	+	+	+	+	+	+	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+	+	+	-	-	-	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Desert

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
5680	tunisian desert		Tunisia	TUN	Africa
67770	Tunisian desert
123361	Environment, Soil	Sahara desert	Tunisia	TUN	Africa

Taxonmaps

69479

Global distribution of 16S sequence AF074970 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
5680	yes, in single cases	1	Risk group (German classification) According to TRBA 466
123361		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM22746v1 assembly for Bacillus spizizenii TU-B-10	complete	GCA_000227465	1052585.4	2511231064	1052585	98.72
66792	ASM798978v1 assembly for Bacillus spizizenii NBRC 101239	contig	GCA_007989785	96241.37		96241	73.86

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Bacillus subtilis subsp. spizizenii strain BCRC 17366 16S ribosomal RNA gene, partial sequence	EF433402	1468	96241
20218	Bacillus subtilis subsp. spizizenii gene for 16S rRNA, partial sequence, strain: NBRC 101239	AB325584	1475	96241
20218	Bacillus subtilis subsp. spizizenii 16S ribosomal RNA gene, partial sequence	AF074970	1409	96241
124043	Bacillus subtilis subsp. spizizenii strain NBRC 101239 16S ribosomal RNA gene, partial sequence.	KR818222	1213	96241
124043	Bacillus subtilis subsp. spizizenii strain NBRC 101239 16S ribosomal RNA gene, partial sequence.	MN526961	498	96241
124043	Bacillus subtilis subsp. spizizenii strain NRRL B-23049 16S ribosomal RNA gene, partial sequence.	MN536905	1508	96241
124043	Bacillus subtilis subsp. spizizenii strain NRRL B-23049 16S ribosomal RNA gene, partial sequence.	EU138464	1168	96241
124043	Bacillus subtilis subsp. spizizenii strain NRRL B-23049 16S ribosomal RNA gene, partial sequence.	MN840038	1408	96241
124043	Bacillus subtilis subsp. spizizenii strain NBRC 101239 16S ribosomal RNA gene, partial sequence.	MT539301	1349	96241

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM22746v1 assembly for Bacillus spizizenii TU-B-10
NCBI: GCA_000227465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	50.94	no
125439	gram_stain	BacteriaNetⓘ	variable	58.38	no
125439	motility	BacteriaNetⓘ	yes	43.42	no
125439	spore_formation	BacteriaNetⓘ	yes	58.20	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Antibiotic profiling of wild-type bacilli led to the discovery of new lanthipeptide subtilin-producing Bacillus spizizenii strains whose 16S rDNA sequences differ from the B. spizizenii typing strain.	Helfrich M, Entian KD, Stein T.	Int Microbiol	10.1007/s10123-022-00266-5	2022
	Isolation and Characterization of the Zearalenone-Degrading Strain, Bacillus spizizenii B73, Inspired by Esterase Activity.	Liu X, Wu N, Zhang M, Xue F, Xu Q.	Toxins (Basel)	10.3390/toxins15080488	2023
Genetics	Genomic insights into the taxonomic status and bioactive gene cluster profiling of Bacillus velezensis RVMD2 isolated from desert rock varnish in Ma'an, Jordan.	Alnaimat SM, Abushattal S, Dmour SM, Al-Awaida WJ, Ayyash AM, Goh KW.	PLoS One	10.1371/journal.pone.0319345	2025
	Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables.	Acuna JJ, Hu J, Inostroza NG, Valenzuela T, Perez P, Epstein S, Sessitsch A, Zhang Q, Jorquera MA.	Sci Rep	10.1038/s41598-023-47099-4	2023
	Antimicrobial Potential of Secondary Metabolites Produced by Bacillus sp. and Their Gas Chromatography (GC)-Mass Spectrometry (MS) Analysis.	Sujitha JWR, Senthilkumar D, Nandhagopal M.	Cureus	10.7759/cureus.70472	2024
Phylogeny	Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.	Bhattacharya D, de Los Santos Villalobos S, Ruiz VV, Selvin J, Mukherjee J	Antonie Van Leeuwenhoek	10.1007/s10482-020-01472-9	2020

References

#5680	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15029
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39469	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123361	Collection of Institut Pasteur ; Curators of the CIP; CIP 106094
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Bacillus spizizenii (DSM 15029, NRRL B-23049, TU-B-10)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000227465

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media