Strain identifier

BacDive ID: 1193

Type strain: Yes

Species: Bacillus spizizenii

Strain Designation: TU-B-10

Strain history: CIP <- 1999, L.K. Nakamura, NCAUR, Illinois, USA <- F.M. Cohan: strain TU-B-10

NCBI tax ID(s): 96241 (species)

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General

@ref: 5680

BacDive-ID: 1193

DSM-Number: 15029

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, human pathogen

description: Bacillus spizizenii TU-B-10 is an obligate aerobe, spore-forming, mesophilic human pathogen that was isolated from tunisian desert.

NCBI tax id

  • NCBI tax id: 96241
  • Matching level: species

strain history

@refhistory
5680<- L. K. Nakamura, NRRL
67770NRRL B-23049 <-- F. M. Cohan TU-B-10.
123361CIP <- 1999, L.K. Nakamura, NCAUR, Illinois, USA <- F.M. Cohan: strain TU-B-10

doi: 10.13145/bacdive1193.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus spizizenii
  • full scientific name: Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus subtilis subsp. spizizenii

@ref: 5680

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus spizizenii

full scientific name: Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

strain designation: TU-B-10

type strain: yes

Morphology

cell morphology

  • @ref: 123361
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 123361
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5680NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
39469MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123361CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123361CIP Medium 316yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=316

culture temp

@refgrowthtypetemperaturerange
5680positivegrowth30mesophilic
39469positivegrowth30mesophilic
67770positivegrowth30mesophilic
123361positivegrowth22-37
123361nogrowth10psychrophilic
123361nogrowth45thermophilic
123361nogrowth55thermophilic

culture pH

  • @ref: 123361
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 123361
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 123361
  • spore formation: yes

halophily

  • @ref: 123361
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12336116947citrate-carbon source
1233614853esculin+hydrolysis
123361606565hippurate-hydrolysis
12336117632nitrate+reduction
12336116301nitrite-reduction
12336117632nitrate-respiration

metabolite production

  • @ref: 123361
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12336115688acetoin+
12336117234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
123361oxidase+
123361beta-galactosidase+3.2.1.23
123361alcohol dehydrogenase-1.1.1.1
123361gelatinase+
123361amylase+
123361DNase+
123361caseinase+3.4.21.50
123361catalase+1.11.1.6
123361tween esterase+
123361gamma-glutamyltransferase+2.3.2.2
123361lecithinase-
123361lipase-
123361lysine decarboxylase-4.1.1.18
123361ornithine decarboxylase-4.1.1.17
123361protease+
123361urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123361-+-+-+----++-+-++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123361++-++-+++++----++++++++-----+--++++++----+-++----++--+-+---++------------+-+--+++-----+--++++++----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5680tunisian desertTunisiaTUNAfrica
67770Tunisian desert
123361Environment, SoilTunisiaTUNAfricaSahara desert

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Desert

taxonmaps

  • @ref: 69479
  • File name: preview.99_21.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_17;97_17;98_20;99_21&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AF074970
  • Sequence Identity:
  • Total samples: 18201
  • soil counts: 3768
  • aquatic counts: 2854
  • animal counts: 9674
  • plant counts: 1905

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
5680yes, in single cases1Risk group (German classification)
1233611Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus subtilis subsp. spizizenii strain BCRC 17366 16S ribosomal RNA gene, partial sequenceEF4334021468ena96241
20218Bacillus subtilis subsp. spizizenii gene for 16S rRNA, partial sequence, strain: NBRC 101239AB3255841475ena96241
20218Bacillus subtilis subsp. spizizenii 16S ribosomal RNA gene, partial sequenceAF0749701409ena96241
20218Bacillus subtilis subsp. spizizenii strain NRRL B-23049 16S ribosomal RNA gene, partial sequenceEU1384641168ena96241

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus spizizenii NBRC 101239GCA_007989785contigncbi96241
66792Bacillus subtilis subsp. spizizenii strain NBRC 10123996241.37wgspatric96241
66792Bacillus subtilis spizizenii TU-B-10, DSM 150292511231064completeimg1052585
67770Bacillus spizizenii TU-B-10GCA_000227465completencbi1052585

External links

@ref: 5680

culture collection no.: DSM 15029, NRRL B-23049, JCM 12233, CIP 106094, LMG 19156, NBRC 101239

straininfo link

  • @ref: 70850
  • straininfo: 14146

literature

  • topic: Phylogeny
  • Pubmed-ID: 32939598
  • title: Bacillus rugosus sp. nov. producer of a diketopiperazine antimicrobial, isolated from marine sponge Spongia officinalis L.
  • authors: Bhattacharya D, de Los Santos Villalobos S, Ruiz VV, Selvin J, Mukherjee J
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-020-01472-9
  • year: 2020
  • mesh: Animals, Anti-Infective Agents/*metabolism, Bacillus/classification/genetics/*isolation & purification/*metabolism, Bacterial Typing Techniques, DNA, Bacterial/genetics, Diketopiperazines/*metabolism, Lipids/analysis, Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5680Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15029)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15029
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39469Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18185
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70850Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14146.1StrainInfo: A central database for resolving microbial strain identifiers
123361Curators of the CIPCollection of Institut Pasteur (CIP 106094)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106094