Bacillus spizizenii (DSM 618, CIP 107063)

Name: Bacillus spizizenii (DSM 618, CIP 107063)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1188

Type strain

Culture col. no. DSM 618 CIP 107063

NCBI tax ID(s) 96241

Special Collections DSMZ CIP

History DSMZ <- Inst. Veterinärmed. Bundesgesundheitsamt Berlin, BGA CIP <- 2001, C. Creff-Froger, AFSSA, Fougères, France: strain BGA, Bacillus subtilis

Links

Bacillus spizizenii DSM 618 is an obligate aerobe, Gram-positive, motile bacterium of the family Bacillaceae.

Gram-positive motile rod-shaped obligate aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13196

LMG 19545,DSM 618,NCIMB 11232,CCM 4062,CECT 482,CIP 107063,CNCTC 6218,VTT E-94565

@ref 20215

Last LPSN update 2026-05-29 08:25:12

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus spizizenii

Full scientific name Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

Synonyms (1)

Bacillus subtilis subsp. spizizenii

BacDive ID	Other strains from **Bacillus spizizenii** (7)	Type strain
1193	B. spizizenii TU-B-10, DSM 15029, NRRL B-23049, JCM 12233, ... (type strain)
1187	B. spizizenii 231, NRS-231, DSM 347, ATCC 6633, CCM 1999, ...
1189	B. spizizenii W 23, DSM 1087
1190	B. spizizenii W23 2A2, 2A2, DSM 6395, BGSC 2A2, WB 672
1191	B. spizizenii SB 623, DSM 6399, BGSC 2A1
1192	B. spizizenii W23 SR, DSM 6405, BGSC 2A3
23702	B. spizizenii W23, DSM 8439, CCM 2268, IAM 12021, JCM 20354, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
123360	positive	rod-shaped

Colony morphology

123360

Hemolysis ability0

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
323	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35001	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123360	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
323	positive	growth	30
35001	positive	growth	30
123360	positive	growth	22-45
123360	negative	growth	10
123360	negative	growth	55

Culture pH

@ref	Ability	Type	PH
123360	positive	growth	6

Physiology and metabolism

Oxygen tolerance

123360

Oxygen toleranceobligate aerobe

Antibiotic susceptibility testing

	Test 1	Test 2	Test 3	Test 4	Test 5	Test 6
@ref	323	323	323	323	323	323
Medium	Mueller-Hinton Agar	Mueller-Hinton Agar	Mueller-Hinton Agar	Mueller-Hinton Agar	Mueller-Hinton Agar	Müller-Hinton Agar
Manual annotation	1	1	1	1	1	1
	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm
Amikacin 30µg (disc)	30	32	24	34	34	30
Ampicillin 10µg (disc)	40	40	28-30	42	42	40
Aztreonam 30µg (disc)	12-14	10	8	18	18	18
Bacitracin 10Unit	8	10-12	6	8	8	n.d.
Cefalotin 30µg (disc)	50	50	48	>50	>50	n.d.
Cefazolin 30µg (disc)	44	42-44	42	46	46	n.d.
Cefiderocol 30µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	20
Cefotaxime 30µg (disc)	36-38	40	30	40	40	42
Ceftazidime 10µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	26-28
Ceftriaxone 30µg (disc)	36	36	n.d.	38	38	40
Chloramphenicol 30µg (disc)	38	36	28	40	40	36-38
Ciprofloxacin 5µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	40
Clindamycin 10µg (disc)	34-36	34-36	n.d.	36	36	32
Colistin 10µg (disc)	10	10	8	10	10	n.d.
Colistin sulphate 10µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	12
Doxycycline 30µg (disc)	40	40	30	42	42	n.d.
Erythromycin 15µg (disc)	36	34-36	28	38	38	36
Fosfomycin 50µg (disc)	20	12-14	n.d.	22-24	22-24	28-30
Gentamicin 10µg (disc)	30	30	22	30	30	n.d.
Gentamicin 30µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	28
Imipenem 10µg (disc)	50	50	46	>50	>50	50
Kanamycin 30µg (disc)	32	32	26	36	36	30
Levofloxacin 5µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	40
Lincomycin 15µg (disc)	22-24	24	14	22-24	22-24	n.d.
Linezolid 10µg (disc)	40	40	n.d.	44	44	42
Meropenem 10µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	46
Mezlocillin 30µg (disc)	34	34	26	40-42	40-42	n.d.
Moxifloxacin 5µg (disc)	40	40	n.d.	42	42	40
Neomycin 30µg (disc)	26	26	24	28	28	n.d.
Nitrofurantoin 100µg (disc)	24	24-26	20	28	28	28
Norfloxacin 10µg (disc)	34	34-36	30	38	38	n.d.
Nystatin 100Unit	0	0	n.d.	0	0	n.d.
Ofloxacin 5µg (disc)	38	36	0	38	38	38
Oxacillin 5µg (disc)	34	34	28	36	36	36
Penicillin G 6µg (disc)	40	40	28-30	44	44	42
Pipemidic acid 20µg (disc)	24	24-26	20	26	26	n.d.
Piperacillin/Tazobactam 40µg (disc)	36	36	n.d.	38	38	n.d.
Piperacillin/Tazobactam 110µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	38
Polymyxin B 300Unit	14-16	16	12	16	16	16
Quinupristin/Dalfopristin 15µg (disc)	22	20-22	n.d.	24	24	26
Rifampicin 5µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	30
Teicoplanin 30µg (disc)	24	22-24	n.d.	24-26	24-26	24-26
Tetracycline 30µg (disc)	38	38	30	40	40	40
Ticarcillin 75µg (disc)	44	44	30-32	48	48	44
Tigecycline 15µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	30
Trimethoprim-sulfamethoxazole (1:19) 10µg (disc)	n.d.	n.d.	n.d.	n.d.	n.d.	42
Vancomycin 30µg (disc)	28	26	22	28	28	28

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123360	NaCl	positive	growth	0-10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123360	16947 ChEBI	citrate	-	carbon source
123360	4853 ChEBI	esculin	+	hydrolysis
123360	606565 ChEBI	hippurate	-	hydrolysis
123360	17632 ChEBI	nitrate	+	reduction
123360	17632 ChEBI	nitrate	-	respiration
123360	16301 ChEBI	nitrite	-	reduction

Antibiotic resistance

@ref	ChEBI	Metabolite	Is resistant	Resistance conc.
323	7660	Nystatin		100 Unit	from Antibiotic test

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123360	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123360	15688 ChEBI	acetoin	+
123360	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123360	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123360	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123360	beta-galactosidase	+	3.2.1.23
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123360	caseinase	+	3.4.21.50
123360	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123360	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123360	gamma-glutamyltransferase	+	2.3.2.2
123360	gelatinase	+/-
123360	lecithinase	-
68382	leucine arylamidase	-	3.4.11.1	from API zym
123360	lipase	-
68382	lipase (C 14)	-		from API zym
123360	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
123360	ornithine decarboxylase	-	4.1.1.17
123360	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
123360	tween esterase	-
123360	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123360	-	-	+	+	-	-	-	-	-	-	+	-	-	-	-	+	+	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123360	not determinedn.d.	+	+	-	+	+	-	+	+	-	+	+	-	-	-	-	+	+	+	+	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	-	+	-	-	-	-	+	-	+	+	-	-	-	-	+	-	-	-	+	-	+	-	-	-	+	+	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	+	+	-	+	-	-	-	-	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence DQ529249 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
323	yes, in single cases	1	Risk group (German classification) According to TRBA 466
123360		1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Bacillus subtilis strain DSM 618 16S ribosomal RNA gene, partial sequence	DQ529249	1379		1423

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Transcriptome	Disparate Effects of Two Clerodane Diterpenes of Giant Goldenrod (Solidago gigantea Ait.) on Bacillus spizizenii.	Bozso Z, Lapat V, Ott PG, Moricz AM.	Int J Mol Sci	10.3390/ijms25031531	2024
Phylogeny	Antimicrobial Diterpenes from Rough Goldenrod (Solidago rugosa Mill.).	Baglyas M, Ott PG, Schwarczinger I, Nagy JK, Darcsi A, Bakonyi J, Moricz AM.	Molecules	10.3390/molecules28093790	2023
Phylogeny	Antibiotic profiling of wild-type bacilli led to the discovery of new lanthipeptide subtilin-producing Bacillus spizizenii strains whose 16S rDNA sequences differ from the B. spizizenii typing strain.	Helfrich M, Entian KD, Stein T	Int Microbiol	10.1007/s10123-022-00266-5	2022

References

#323	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 618
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#35001	; Curators of the CIP;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123360	Collection of Institut Pasteur ; Curators of the CIP; CIP 107063
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bacillus spizizenii (DSM 618, CIP 107063)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Antibiotic susceptibility testing

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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