Name: Komagataeibacter xylinus Blackley strain G2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 89

Type strain:

Species: Komagataeibacter xylinus

Strain Designation: Blackley strain G2, BU 335

Culture col. no.: DSM 46604, ATCC 10245, CCM 2360, CIP 109889, IFO 3173, IMET 10315, LBG 4113, NBRC 3173, NCIB 8034, NRC 6018

Strain history: CIP <- 2008, <- DSMZ <- IMET <- CCM <- NRC <- ATCC <- L.C. Stewart <- R.H. Vaughn

NCBI tax ID(s): 28448 (species)

SI-ID 92464

LMG 1522, ATCC 10245, CCM 2360, IMET 10315, NCIB 8034, DSM 46604, NCIMB 8034, BCRC 12951, CCRC 12951, CDBB 538, NRRL B-759, CIP 109889, NBRC 3173

Other strains from Komagataeibacter xylinus

K. xylinus DSM 6513, ATCC 23767, BCC 7226, ... (type strain)
K. xylinus DSM 2325, ATCC 11142, NCIB 5346, ...
K. xylinus B 4168, DSM 2004, ATCC 23768, NCIB ...
K. xylinus East Africa, DSM 46605, ATCC 14851, ...
K. xylinus DSM 46590, IMET 10141
K. xylinus DSM 46591, IMET 10142
K. xylinus BU 134, DSM 46603, ATCC 10821, ...
K. xylinus JCM 10150
K. xylinus JCM 7664, IFO 13693, NBRC 13693

Section

Komagataeibacter xylinus Blackley strain G2 is a mesophilic, Gram-negative, rod-shaped bacterium of the family Acetobacteraceae.

Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Komagataeibacter*
	Species	**Komagataeibacter xylinus**
	Full Scientific Name (LPSN)	Komagataeibacter xylinus (Brown 1886) Yamada et al. 2013

	Synonym	"Komagatabacter* xylinus"*
	Synonym	"Bacterium* xylinum"*
	Synonym	Acetobacter xylinus
	Synonym	Gluconoacetobacter xylinus
	Synonym	Acetobacter aceti subsp. xylinus
	Synonym	Acetobacter xylinum
	Synonym	Acetobacter aceti subsp. xylinum
	Synonym	Gluconacetobacter xylinus

Information on morphological and physiological properties Morphology

[Ref.: #124000]	Gram stain	negative

[Ref.: #124000]	Cell shape	rod-shaped

[Ref.: #124000]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12583]

Culture medium

GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)

[Ref.: #12583]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #12583]

Culture medium composition

expand / minimize

[Ref.: #32700]

Culture medium

MEDIUM 314 - for Gluconacetobacter xylinus subsp. sucrofermentans

[Ref.: #32700]

Culture medium growth

[Ref.: #32700]

Culture medium composition

expand / minimize

[Ref.: #124000]

Culture medium

CIP Medium 314

[Ref.: #124000]

Culture medium growth

[Ref.: #124000]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #12583]			growth	28 °C
[Ref.: #32700]			growth	30 °C

[Ref.: #12583]	Temperature range	mesophilic
[Ref.: #32700]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #12583]

Name of produced compound

2 keto-L-gulonic acid

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #124000]		nitrate ChEBI	-	reduction
[Ref.: #124000]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #124000]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #124000]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #124000]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #124000]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #124000]	12195	-	-	+	-	-	+	+	-	-	-	+	+	-	-	-	-	+	+	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12583]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #124000]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Komagataeibacter xylinus partial 16S rRNA gene, strain DSM 46604	AJ316552	587	ENA	28448 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Komagataeibacter oboediens NCIB 8034	GCA_029229465	complete	GenBank	65958 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Komagataeibacter oboediens NCIB 8034 NCBI: GCA_029229465.1
[Ref.: #69480]	flagellated	no	98.355	no
[Ref.: #69480]	gram-positive	no	92.122	no
[Ref.: #69480]	anaerobic	no	94.763	no
[Ref.: #69480]	aerobic	yes	62.462	no
[Ref.: #69480]	halophile	no	91.355	no
[Ref.: #69480]	spore-forming	no	96.683	no
[Ref.: #69480]	glucose-ferment	no	83.029	no
[Ref.: #69480]	thermophile	no	92.143	yes
[Ref.: #69480]	glucose-util	yes	86.502	no
[Ref.: #69480]	motile	no	81.866	no

Availability in culture collections External links

[Ref.: #12583]

Culture collection no.

DSM 46604, ATCC 10245, CCM 2360, CIP 109889, IFO 3173, IMET 10315, LBG 4113, NBRC 3173, NCIB 8034, NRC 6018

[Ref.: #69772]

SI-ID 92464

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Metabolism	Vitamin C enhances bacterial cellulose production in Gluconacetobacter xylinus.	Keshk SM	Carbohydr Polym	10.1016/j.carbpol.2013.08.060	2013	*
Metabolism	Direct incorporation of glucosamine and N-acetylglucosamine into exopolymers by Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245: production of chitosan-cellulose and chitin-cellulose exopolymers.	Lee JW, Deng F, Yeomans WG, Allen AL, Gross RA, Kaplan DL	Appl Environ Microbiol	10.1128/AEM.67.9.3970-3975.2001	2001	*
Metabolism	Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha.	Semjonovs P, Ruklisha M, Paegle L, Saka M, Treimane R, Skute M, Rozenberga L, Vikele L, Sabovics M, Cleenwerck I	Appl Microbiol Biotechnol	10.1007/s00253-016-7761-8	2016	*
Metabolism	CP/MAS (13)C NMR study of cellulose and cellulose derivatives. 2. Complete assignment of the (13)C resonance for the ring carbons of cellulose triacetate polymorphs.	Kono H, Erata T, Takai M	J Am Chem Soc	10.1021/ja010705g	2002	*
Metabolism	CP/MAS (13)C NMR study of cellulose and cellulose derivatives. 1. Complete assignment of the CP/MAS (13)C NMR spectrum of the native cellulose.	Kono H, Yunoki S, Shikano T, Fujiwara M, Erata T, Takai M	J Am Chem Soc	10.1021/ja010704o	2002	*
	Synthesized OH-radical rich bacteria cellulosic pockets with photodynamic bacteria inactivation properties against S. ureus and E. coli.	Narh C, Badoe W, Howard EK, Lin NX, Mensah A, Wang T, Wang Q, Huang F, Wei Q	Mater Sci Eng C Mater Biol Appl	10.1016/j.msec.2020.111230	2020	*
	Cross-polarization/magic-angle spinning 13C nuclear magnetic resonance study of cellulose I-ethylenediamine complex.	Numata Y, Kono H, Kawano S, Erata T, Takai M	J Biosci Bioeng	10.1016/S1389-1723(03)70132-7	2003	*

References

#12583

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46604

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32700 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69772

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92464.1 )

#124000 Collection of Institut Pasteur ; Curators of the CIP; CIP 109889
* These data were automatically processed and therefore are not curated

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