Strain identifier

BacDive ID: 89

Type strain: No

Species: Komagataeibacter xylinus

Strain Designation: Blackley strain G2, BU 335

Strain history: CIP <- 2008, <- DSMZ <- IMET <- CCM <- NRC <- ATCC <- L.C. Stewart <- R.H. Vaughn

NCBI tax ID(s): 28448 (species)

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General

@ref: 12583

BacDive-ID: 89

DSM-Number: 46604

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped

description: Komagataeibacter xylinus Blackley strain G2 is a mesophilic, Gram-negative, rod-shaped bacterium of the family Acetobacteraceae.

NCBI tax id

  • NCBI tax id: 28448
  • Matching level: species

strain history

@refhistory
12583<- IMET <- CCM <- NRC <- ATCC <- L.C. Stewart <- R.H. Vaughn
124000CIP <- 2008, <- DSMZ <- IMET <- CCM <- NRC <- ATCC <- L.C. Stewart <- R.H. Vaughn

doi: 10.13145/bacdive89.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Komagataeibacter
  • species: Komagataeibacter xylinus
  • full scientific name: Komagataeibacter xylinus (Brown 1886) Yamada et al. 2013

  • synonyms

    @refsynonym
    20215Komagatabacter xylinus
    20215Bacterium xylinum
    20215Acetobacter xylinus
    20215Gluconoacetobacter xylinus
    20215Acetobacter aceti subsp. xylinus
    20215Acetobacter xylinum
    20215Acetobacter aceti subsp. xylinum
    20215Gluconacetobacter xylinus

@ref: 12583

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Komagataeibacter

species: Komagataeibacter xylinus

full scientific name: Komagataeibacter xylinus (Brown 1886) Yamada et al. 2013

strain designation: Blackley strain G2, BU 335

type strain: no

Morphology

cell morphology

  • @ref: 124000
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12583GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)yeshttps://mediadive.dsmz.de/medium/105Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water
32700MEDIUM 314 - for Gluconacetobacter xylinus subsp. sucrofermentansyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (100.000 g);Yeast extract (10.000 g);Calcium carbonate (30.000 g)
124000CIP Medium 314yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=314

culture temp

@refgrowthtypetemperaturerange
12583positivegrowth28mesophilic
32700positivegrowth30mesophilic

Physiology and metabolism

compound production

  • @ref: 12583
  • compound: 2 keto-L-gulonic acid

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12400017632nitrate-reduction
12400016301nitrite-reduction

metabolite production

  • @ref: 124000
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
124000oxidase-
124000catalase+1.11.1.6
124000urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
124000--+--++---++----++--

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
125831Risk group (German classification)
1240001Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Komagataeibacter xylinus partial 16S rRNA gene, strain DSM 46604
  • accession: AJ316552
  • length: 587
  • database: ena
  • NCBI tax ID: 28448

Genome sequences

  • @ref: 66792
  • description: Komagataeibacter oboediens NCIB 8034
  • accession: GCA_029229465
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 65958

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno98.355no
gram-positiveno92.122no
anaerobicno94.763no
aerobicyes62.462no
halophileno91.355no
spore-formingno96.683no
glucose-fermentno83.029no
thermophileno92.143yes
glucose-utilyes86.502no
motileno81.866no

External links

@ref: 12583

culture collection no.: DSM 46604, ATCC 10245, CCM 2360, CIP 109889, IFO 3173, IMET 10315, LBG 4113, NBRC 3173, NCIB 8034, NRC 6018

straininfo link

  • @ref: 69772
  • straininfo: 92464

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism11525993Direct incorporation of glucosamine and N-acetylglucosamine into exopolymers by Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245: production of chitosan-cellulose and chitin-cellulose exopolymers.Lee JW, Deng F, Yeomans WG, Allen AL, Gross RA, Kaplan DLAppl Environ Microbiol10.1128/AEM.67.9.3970-3975.20012001Acetylglucosamine/*metabolism, Cellulose/metabolism, Chitin/analogs & derivatives/metabolism, Chitosan, Gluconacetobacter xylinus/growth & development/*metabolism, Glucosamine/*metabolism, Polymers/chemistry/isolation & purification/*metabolismBiotechnology
Metabolism12071760CP/MAS (13)C NMR study of cellulose and cellulose derivatives. 1. Complete assignment of the CP/MAS (13)C NMR spectrum of the native cellulose.Kono H, Yunoki S, Shikano T, Fujiwara M, Erata T, Takai MJ Am Chem Soc10.1021/ja010704o2002Acetobacter/metabolism, Carbon Isotopes, Cellulose/analogs & derivatives/biosynthesis/*chemistry, Chlorophyta/chemistry, Glucose/chemistry/metabolism, Glycerol/chemistry/metabolism, Isotope Labeling, Nuclear Magnetic Resonance, Biomolecular/methods
Metabolism12071761CP/MAS (13)C NMR study of cellulose and cellulose derivatives. 2. Complete assignment of the (13)C resonance for the ring carbons of cellulose triacetate polymorphs.Kono H, Erata T, Takai MJ Am Chem Soc10.1021/ja010705g2002Acetobacter/metabolism, Carbon Isotopes, Cellulose/*analogs & derivatives/biosynthesis/*chemistry, Crystallography, X-Ray, Glucose/metabolism, Isotope Labeling, Nuclear Magnetic Resonance, Biomolecular/methods
16233556Cross-polarization/magic-angle spinning 13C nuclear magnetic resonance study of cellulose I-ethylenediamine complex.Numata Y, Kono H, Kawano S, Erata T, Takai MJ Biosci Bioeng10.1016/S1389-1723(03)70132-72003
Metabolism24274484Vitamin C enhances bacterial cellulose production in Gluconacetobacter xylinus.Keshk SMCarbohydr Polym10.1016/j.carbpol.2013.08.0602013Ascorbic Acid/metabolism/*pharmacology, Cellulose/*biosynthesis/chemistry, Crystallization, Culture Media, Fermentation, Gluconacetobacter xylinus/drug effects/*metabolism, Gluconates/metabolism, Magnetic Resonance Spectroscopy, Polysaccharides, Bacterial/*biosynthesis/chemistry, Spectroscopy, Fourier Transform InfraredCultivation
Metabolism27678116Cellulose synthesis by Komagataeibacter rhaeticus strain P 1463 isolated from Kombucha.Semjonovs P, Ruklisha M, Paegle L, Saka M, Treimane R, Skute M, Rozenberga L, Vikele L, Sabovics M, Cleenwerck IAppl Microbiol Biotechnol10.1007/s00253-016-7761-82016Amplified Fragment Length Polymorphism Analysis, Carbon/metabolism, Cellulose/*biosynthesis/metabolism, Culture Media/chemistry, *Fermentation, Gluconacetobacter/classification/growth & development/isolation & purification/*metabolism, Glucose/metabolism, Kombucha Tea/*microbiologyPhylogeny
32806321Synthesized OH-radical rich bacteria cellulosic pockets with photodynamic bacteria inactivation properties against S. ureus and E. coli.Narh C, Badoe W, Howard EK, Lin NX, Mensah A, Wang T, Wang Q, Huang F, Wei QMater Sci Eng C Mater Biol Appl10.1016/j.msec.2020.1112302020Bacteria, Bacterial Outer Membrane Proteins, Cellulose, Escherichia coli, *Escherichia coli Proteins, *Gluconacetobacter xylinus, Staphylococcus aureus

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12583Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46604)https://www.dsmz.de/collection/catalogue/details/culture/DSM-46604
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32700Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7684
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69772Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92464.1StrainInfo: A central database for resolving microbial strain identifiers
124000Curators of the CIPCollection of Institut Pasteur (CIP 109889)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109889