Name: ISP 3; 5315
Composition
Dog oat flakes 20.0 g/l
Trace element solution (5314) 2.5 ml/l
Agar 18.0 g/l
Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated).
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.8
Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production)
Organisms: All Actinomycetes
Trace element solution 5314
Name: Trace element solution 5314; 5314
Composition
CaCl2 x H2O 3.0 g/l
Fe-III-citrate 1.0 g/l
MnSO4 0.2 g/l
ZnCl2 0.1 g/l
CuSO4 x 5 H2O 0.025 g/l
Sodium tetra borate 0.2 g/l
CoCl2 x 6 H2O 0.004 g/l
Sodium molybdate 0.01 g/l
Preparation: Use double destillated water.
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Usage: Trace element solution for different media
Organisms:
Solution I:
Difco soluble starch, 10.0 g.
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see below) 1.0 ml
The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
Trace element solution:
FeSO4 x 7 H2O 0.1 g
MnCl2 x 4 H2O 0.1 g
ZnSO4 x 7 H2O 0.1 g
Distilled water 100.0 ml
Name: ISP 5 (5323)
Composition
L-Asparagine 1.0 g/l
Glycerol 10.0 g/l
K2HPO4 1.0 g/l
Salt solution (see preparation) 1.0 ml/l
Agar 20.0 g/l
Preparation:
Salt solution
1.0 g FeSO4 x 7 H2O
1.0 g MnCl2 x 4 H2O
1.0 g ZNSO4 x 7 H2O
in 100 ml water
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.2
Usage:
Maintenance and taxonomy
Organisms:
All Actinomycetes
Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
Purification and N-terminal amino-acid sequences of bacterial malate dehydrogenases from six actinomycetales strains and from Phenylobacterium immobile, strain E.
Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431(T) (= NBRC 102363(T)).
Yamamura H, Ohnishi Y, Ishikawa J, Ichikawa N, Ikeda H, Sekine M, Harada T, Horinouchi S, Otoguro M, Tamura T, Suzuki K, Hoshino Y, Arisawa A, Nakagawa Y, Fujita N, Hayakawa M
Isolation, structural elucidation and biosynthesis of 3-hydroxy-6-dimethylallylindolin-2-one, a novel prenylated indole derivative from Actinoplanes missouriensis.
Satou R, Izumikawa M, Katsuyama Y, Matsui M, Takagi M, Shin-ya K, Ohnishi Y
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368
Automatically annotated from API 20E .
#68382
Automatically annotated from API zym .
* These data were automatically processed and therefore are not curated
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