Strain identifier

BacDive ID: 7809

Type strain: Yes

Species: Actinoplanes missouriensis

Strain Designation: UNCC 431

Strain history: KCC A-0121 <-- A. Domnas UNCC 431.

NCBI tax ID(s): 1866 (species)

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General

@ref: 10716

BacDive-ID: 7809

DSM-Number: 43046

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Actinoplanes missouriensis UNCC 431 is an aerobe, spore-forming, mesophilic bacterium that builds sporangium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1866
  • Matching level: species

strain history

@refhistory
10716<- KCC <- A. Domnas, UNCC
67770KCC A-0121 <-- A. Domnas UNCC 431.

doi: 10.13145/bacdive7809.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes missouriensis
  • full scientific name: Actinoplanes missouriensis Couch 1963 (Approved Lists 1980)

@ref: 10716

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes missouriensis

full scientific name: Actinoplanes missouriensis Couch 1963 emend. Nouioui et al. 2018

strain designation: UNCC 431

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

colony morphology

@refcolony colorincubation periodmedium usedcolony shape
19539Pastel yellow10-14 daysISP 2
19539Brown10-14 daysISP 3
19539Pastel yellow10-14 daysISP 4
19539Pastel yellow10-14 daysISP 5
19539Salmon red10-14 daysISP 6
19539Salmon red10-14 daysISP 7
43421ochreCzapek agarrhizoid

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
19539noISP 2
19539noISP 3
19539noISP 4
19539noISP 5
19539noISP 6
19539noISP 7
43421yesczapek agarsporangiumochre

multimedia

@refmultimedia contentintellectual property rightscaption
10716https://www.dsmz.de/microorganisms/photos/DSM_43046-1.jpg© Leibniz-Institut DSMZ
10716https://www.dsmz.de/microorganisms/photos/DSM_43046.jpg© Leibniz-Institut DSMZMedium 215 28°C

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
19539ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19539ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19539ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19539ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19539ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19539ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
43421Czapek agaryes30g Saccharose, 2g Sodium Nitrate, 1g Dipotassium Phosphate, 0.5g Magnesium Sulfate, 0.5g Potassium Chloride, 0.01 Ferrous Sulfate, 15g Agar, ad 1000mL Aqua dest.
10716BHI MEDIUM (DSMZ Medium 215)yesName: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/215
10716GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yesName: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/65

culture temp

@refgrowthtypetemperaturerange
19539positiveoptimum28mesophilic
43421positiveoptimum28mesophilic
10716positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43421
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
19539spore surface: smooth, sporangia: +sporeyes
43421motile spores out of sporangiazoosporeyes
69481yes99
69480yes99.999

halophily

@refsaltgrowthtested relationconcentration
19539NaClpositivemaximum2.5 %
43421NaClpositive2 %

observation

@refobservation
43421Antimicrobial activity against Streptomyces murinus
43421Antimicrobial activity against Aspergillus niger
43421Antimicrobial activity against Bacillus subitilis
43421Antimicrobial activity against Staphylococcus aureus
43421Formation of aerial mycelium
43421natural rubber decomposition
43421flavonoid decomposition
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1953917234glucose+
1953922599arabinose+
1953917992sucrose+
1953918222xylose-
1953917268myo-inositol-
1953929864mannitol+
1953928757fructose+
1953926546rhamnose+
1953916634raffinose-
1953962968cellulose-
4342112936D-galactose+assimilation
43421161933-hydroxybenzoate-assimilation
434213-O-methyl alpha-D-glucopyranoside-assimilation
43421178794-hydroxybenzoate+assimilation
4342115963ribitol-assimilation
4342162968cellulose-assimilation
4342162968cellulose-degradation
4342117029chitin+degradation
4342117108D-arabinose-assimilation
4342115824D-fructose+assimilation
4342117634D-glucose+assimilation
4342117716lactose+assimilation
4342116899D-mannitol+assimilation
4342116024D-mannose+assimilation
434216731melezitose-assimilation
4342116988D-ribose-assimilation
4342117924D-sorbitol-assimilation
4342165327D-xylose+assimilation
4342123652dextrin+assimilation
43421115156disodium fumarate+assimilation
4342116991dna+degradation
434214767elastin+assimilation
4342130849L-arabinose+assimilation
4342162345L-rhamnose+assimilation
4342161995lecithin+assimilation
4342137684mannose+assimilation
43421320055methyl beta-D-glucopyranoside+assimilation
4342117268myo-inositol-assimilation
4342116634raffinose-assimilation
4342133697rna+assimilation
4342132954sodium acetate+assimilation
4342163675sodium succinate-assimilation
4342117992sucrose+assimilation
43421132111syringate-assimilation
4342118186tyrosine+assimilation
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19539+++++++++++++-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19539+---+----++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
10716soilUSAUSANorth America
67770SoilUSAUSANorth Americanear Hamilton, MO

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_2220.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_94;96_240;97_1422;98_1731;99_2220&stattab=map
  • Last taxonomy: Actinoplanes
  • 16S sequence: AJ277572
  • Sequence Identity:
  • Total samples: 731
  • soil counts: 429
  • aquatic counts: 75
  • animal counts: 129
  • plant counts: 98

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
107161Risk group (German classification)
195391Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinoplanes missouriensis gene for 16S rRNA, partial sequenceAB0370081444ena1866
20218Actinoplanes missouriensis partial 16S rRNA gene, strain IMSNU 22051TAJ2775721484ena1866
20218Actinoplanes missouriensis gene for 16S rRNA, partial sequenceAB7119141470ena1866

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinoplanes missouriensis 431512565.3completepatric512565
66792Actinoplanes missouriensis NBRC 1023632513237176completeimg512565
67770Actinoplanes missouriensis 431GCA_000284295completencbi512565

GC content

@refGC-contentmethod
4342170.82
6777070.82genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes99no
gram-positiveyes91.097no
anaerobicno99.163yes
halophileno96.382no
spore-formingyes93.463no
glucose-utilyes91.051yes
aerobicyes93.311no
thermophileno99.414no
motileyes67.421no
flagellatedno89.728no
glucose-fermentno89.677no

External links

@ref: 10716

culture collection no.: DSM 43046, ATCC 14538, CBS 188.64, IFO 13243, KCC A-0121, NBRC 13243, JCM 3121, BCRC 13335, CGMCC 4.1488, IFM 1250, IMET 9249, IMSNU 22051, KCTC 1780, NBIMCC 2232, NBRC 102363, NCIMB 12654, NRRL B-3342, VTT E-82151

straininfo link

  • @ref: 77251
  • straininfo: 88637

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology2775496Purification and N-terminal amino-acid sequences of bacterial malate dehydrogenases from six actinomycetales strains and from Phenylobacterium immobile, strain E.Rommel TO, Hund HK, Speth AR, Lingens FBiol Chem Hoppe Seyler10.1515/bchm3.1989.370.2.7631989Actinomycetales/*enzymology, Amino Acid Sequence, Ammonium Sulfate, Chromatography, Affinity, Chromatography, DEAE-Cellulose, Chromatography, Ion Exchange, Culture Media, Electrophoresis, Polyacrylamide Gel, Gram-Negative Bacteria/*enzymology, Malate Dehydrogenase/*analysis/isolation & purification, Molecular Sequence DataCultivation
Metabolism16343619Identification of linear plasmid pAM1 in the flavonoid degrading strain Actinoplanes missouriensis(T) (DSM 43046).Rose K, Fetzner SPlasmid10.1016/j.plasmid.2005.10.0032005Biodegradation, Environmental, Dioxygenases/metabolism, Electrophoresis, Gel, Pulsed-Field, Flavonoids/*metabolism, Hesperidin/metabolism, Micromonosporaceae/*genetics/metabolism/pathogenicity, Plant Diseases/*microbiology, Plasmids/*genetics, Quercetin/metabolism, Replicon, Rutin/metabolismEnzymology
Genetics23407331Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431(T) (= NBRC 102363(T)).Yamamura H, Ohnishi Y, Ishikawa J, Ichikawa N, Ikeda H, Sekine M, Harada T, Horinouchi S, Otoguro M, Tamura T, Suzuki K, Hoshino Y, Arisawa A, Nakagawa Y, Fujita N, Hayakawa MStand Genomic Sci10.4056/sigs.31965392012
Metabolism24220111Isolation, structural elucidation and biosynthesis of 3-hydroxy-6-dimethylallylindolin-2-one, a novel prenylated indole derivative from Actinoplanes missouriensis.Satou R, Izumikawa M, Katsuyama Y, Matsui M, Takagi M, Shin-ya K, Ohnishi YJ Antibiot (Tokyo)10.1038/ja.2013.1162013Allyl Compounds/chemistry/*isolation & purification/metabolism, Cord Factors/chemistry/*isolation & purification, Dimethylallyltranstransferase/metabolism, Escherichia coli/metabolism, Genome, Bacterial, Indoles/chemistry/*isolation & purification/metabolism, Magnetic Resonance Spectroscopy, Mass Spectrometry, Micromonosporaceae/genetics/*metabolism, Multigene Family, Oxidoreductases/metabolism, Recombinant Proteins/metabolism, Tryptophan/metabolismPhylogeny
Phylogeny24566827Actinoplanes lutulentus sp. nov., isolated from mucky soil in China.Gao R, Liu C, Zhao J, Jia F, Li C, Xing J, Wang X, Xiang WInt J Syst Evol Microbiol10.1099/ijs.0.057802-02014Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics
Phylogeny26552374Actinoplanes lichenis sp. nov., isolated from lichen.Phongsopitanun W, Matsumoto A, Inahashi Y, Kudo T, Mori M, Shiomi K, Takahashi Y, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0007462015Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Lichens/*microbiology, Micromonosporaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Bark, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10716Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43046)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43046
19539Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43046.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43421Hideki Yamamura, Yasuo Ohnishi, Jun Ishikawa, Natsuko Ichikawa, Haruo Ikeda, Mitsuo Sekine, Takeshi Harada, Sueharu Horinouchi, Misa Otoguro, Tomohiko Tamura, Ken-ichiro Suzuki, Yasutaka Hoshino, Akira Arisawa, Youji Nakagawa, No- buyuki Fujita and Masayuki Hayakawa10.4056/sigs.3196539Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431 (= NBRC 102363)Stand Genomic Sci 7: - 201223407331
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77251Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88637.1StrainInfo: A central database for resolving microbial strain identifiers