Actinoplanes lobatus (DSM 43150, ATCC 15350, CBS 192.64)

Name: Actinoplanes lobatus (DSM 43150, ATCC 15350, CBS 192.64)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7808

Type strain

Strain Designation UNCC 72

Culture col. no. DSM 43150 ATCC 15350 CBS 192.64 IFO 12513 KCC A-0061 15 more

NCBI tax ID(s) 113568

Special Collections DSMZ JCM KCTC Wink compendium JMRC

History DSMZ <- KCC <- CBS <- J.N. Couch, UNCC, 72 JCM <-- KCC A-0061 <-- CBS 192.64 <-- J. N. Couch UNCC 72.

Links

Actinoplanes lobatus UNCC 72 is a spore-forming bacterium that was isolated from soil.

spore-forming genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 36343

ATCC 15350,DSM 43150,IFO 12513,JCM 3061,IMET 9246,NCIMB 12888,NRRL B-3346,VKM Ac-676,CBS 192.64,KCTC 9348,NBRC 12513,BCRC 16345,CCRC 16345

@ref 20215

Last LPSN update 2026-05-29 08:26:44

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Actinoplanes

Species Actinoplanes lobatus

Full scientific name Actinoplanes lobatus (Couch 1963) Stackebrandt and Kroppenstedt 1988

Synonyms (2)

Ampullariella lobata
"Ampullaria lobata"

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125439		94.779
125439		99.054	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
19548	Yellow orange	10-14 days	ISP 2
19548	Yellow orange	10-14 days	ISP 3
19548	Yellow orange	10-14 days	ISP 4
19548	Yellow orange	10-14 days	ISP 5
19548	Yellow orange	10-14 days	ISP 6
19548	Yellow orange	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19548		ISP 2
19548		ISP 3
19548		ISP 4
19548		ISP 5
19548		ISP 6
19548		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
19548	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19548	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19548	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19548	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19548	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19548	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10820	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water
10820	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
10820	positive	growth	28
19548	positive	optimum	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.526

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
19548	Spore surface: smooth, sporangia: +	spore
125439				92.317

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19548	NaCl	positive	maximum	2.5 %

Observation

67770

Observationquinones: MK-9(H₄), MK-10(H₄) , MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68368	gelatinase	-		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19548	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
10820	soil
67770	Soil	Madison, WI	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AB037006 (>99% sequence identity) for Actinoplanes from Microbeatlas

Aquatic Samples	2
Soil Samples	8
Animal Samples	2
Plant Samples	5
Total Samples	17

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
19548	1	Risk group (German classification) According to TRBA 466
10820	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1420521v1 assembly for Actinoplanes lobatus DSM 43150	contig	GCA_014205215	113568.3	2873296955	113568	72.37
66792	ASM1686225v1 assembly for Actinoplanes lobatus NBRC 12513	contig	GCA_016862255	113568.5	8110459644	113568	23.95
66792	ASM1464781v1 assembly for Actinoplanes lobatus JCM 3061	scaffold	GCA_014647815	113568.4	8112896841	113568	23.75

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinoplanes lobatus gene for 16S rRNA, partial sequence	AB037006	1472		113568
124043	Actinoplanes lobatus strain JCM 3061 16S ribosomal RNA gene, partial sequence.	MT760395	1321		113568

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1420521v1 assembly for Actinoplanes lobatus DSM 43150
NCBI: GCA_014205215

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.53	no
125439	gram_stain	BacteriaNetⓘ	positive	99.05	no
125439	motility	BacteriaNetⓘ	no	94.78	no
125439	spore_formation	BacteriaNetⓘ	yes	92.32	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinoplanes lobatus JCM 3061
NCBI: GCA_014647815.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.85	no
125438	anaerobic	anaerobicⓘ	no	89.26	no
125438	aerobic	aerobicⓘ	yes	86.55	no
125438	spore-forming	spore-formingⓘ	yes	87.53	yes
125438	thermophilic	thermophileⓘ	no	94.24	no
125438	flagellated	motile2+ⓘ	no	62.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Identification of Two Distinct Stereoselective Lysine 5-Hydroxylases by Genome Mining Based on Alazopeptin Biosynthetic Enzymes.	Kawai S, Moriga K, Nirdnoy W, Hara R, Ogawa J, Katsuyama Y, Ohnishi Y.	Chemistry	10.1002/chem.202404790	2025
	A novel teichuronic acid, the major polymer from the cell wall of Actinoplanes lobatus VKM Ac-676(T.).	Shashkov AS, Streshinskaya GM, Tul'skaya EM, Kozlova YI, Senchenkova SN, Evtushenko LI	Carbohydr Res	10.1016/j.carres.2013.08.031	2013
Phylogeny	Actinoplanes sichuanensis sp. nov. and Actinoplanes xinjiangensis sp. nov.	Sun W, Dong GX, Zhang YQ, Wei YZ, Li QP, Yu LY, Klenk HP, Zhang YQ.	Int J Syst Evol Microbiol	10.1099/ijs.0.010561-0	2009
Phylogeny	Actinoplanes flavus sp. nov., a novel cellulase-producing actinobacterium isolated from coconut palm rhizosphere soil.	Luo X, Sun X, Huang Z, He C, Zhao J, Xiang W, Song J, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.004990	2021
Phylogeny	Actinoplanes hulinensis sp. nov., a novel actinomycete isolated from soybean root (Glycine max (L.) Merr).	Shen Y, Liu C, Wang X, Zhao J, Jia F, Zhang Y, Wang L, Yang D, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-012-9809-9	2012

References

#10820	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43150
#19548	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

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Actinoplanes lobatus (DSM 43150, ATCC 15350, CBS 192.64)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014205215

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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