Name: Nesterenkonia alba DSM 19423
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7771

Type strain:

Species: Nesterenkonia alba

Culture col. no.: DSM 19423, CCTCC AB 207011, JCM 16549, CAAS 252, NBRC 107629

Strain history: DSM 19423 <-- B. Yao <-- B. Yao and H. Luo CAAS 252.

NCBI tax ID(s): 1122602 (strain), 515814 (species)

SI-ID 407660

DSM 19423

Special Collections

Section

Nesterenkonia alba DSM 19423 is a Gram-positive, rod-shaped bacterium that was isolated from black liquor traetment system of cotton pulp mill.

Gram-positive
rod-shaped
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia alba**
	Full Scientific Name (LPSN)	Nesterenkonia alba Luo et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #28995]	Gram stain	positive

[Ref.: #28995]	Cell length	1 µm

[Ref.: #28995]	Cell width	0.5 µm

[Ref.: #28995]	Cell shape	rod-shaped

[Ref.: #28995]	Motility	no

[Ref.: #18336]	Incubation period	10-14 days
[Ref.: #18336]	Cultivation medium used	ISP 4

[Ref.: #18336]	Incubation period	10-14 days
[Ref.: #18336]	Cultivation medium used	ISP 5

[Ref.: #18336]	Incubation period	10-14 days
[Ref.: #18336]	Cultivation medium used	ISP 6

[Ref.: #18336]	Incubation period	10-14 days
[Ref.: #18336]	Cultivation medium used	ISP 7

[Ref.: #18336]	Multicellular complex forming ability	no
[Ref.: #18336]	Medium Name (multicellularity)	ISP 4

[Ref.: #18336]	Multicellular complex forming ability	no
[Ref.: #18336]	Medium Name (multicellularity)	ISP 5

[Ref.: #18336]	Multicellular complex forming ability	no
[Ref.: #18336]	Medium Name (multicellularity)	ISP 6

[Ref.: #18336]	Multicellular complex forming ability	no
[Ref.: #18336]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8097]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8097]

Culture medium growth

[Ref.: #8097]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8097]

Culture medium composition

expand / minimize

[Ref.: #18336]

Culture medium

ISP 4

[Ref.: #18336]

Culture medium growth

[Ref.: #18336]

Culture medium composition

expand / minimize

[Ref.: #18336]

Culture medium

ISP 5

[Ref.: #18336]

Culture medium growth

[Ref.: #18336]

Culture medium composition

expand / minimize

[Ref.: #18336]

Culture medium

ISP 6

[Ref.: #18336]

Culture medium growth

[Ref.: #18336]

Culture medium composition

expand / minimize

[Ref.: #18336]

Culture medium

ISP 7

[Ref.: #18336]

Culture medium growth

[Ref.: #18336]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8097]	growth	37 °C
[Ref.: #18336]	optimum	37 °C
[Ref.: #28995]	growth	20-50 °C
[Ref.: #28995]	optimum	42 °C
[Ref.: #67770]	growth	37 °C

	pH	Kind of pH		pH
[Ref.: #28995]			optimum	9.5
[Ref.: #28995]			growth	08-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28995]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #28995]		NaCl		growth	0-6	%
[Ref.: #28995]		NaCl		optimum	3	%

[Ref.: #8097]	Murein short key	A11.42
[Ref.: #8097]	Murein types	A4alpha L-Lys-Gly-D-Asp

[Ref.: #28995]	Observation	aggregates in clumps
[Ref.: #67770]	Observation	quinones: MK-8, MK-9, MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #28995]	arabinose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #28995]	gelatin ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #28995]	glucose ChEBI	+	carbon source
[Ref.: #28995]	glycerol ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #28995]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #28995]	melibiose ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #28995]	raffinose ChEBI	+	carbon source
[Ref.: #28995]	sucrose ChEBI	+	carbon source
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #28995]	trehalose ChEBI	+	carbon source
[Ref.: #28995]	tween 80 ChEBI	+	carbon source
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #28995]	xylitol ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne
[Ref.: #28995]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18336]	28	-	+	+	-	-	+	-	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18336]	46	not determinedn.d.	-	+	+	-	+	-	-	-	-	+	-	+	+	+	-	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8097]	Sample type/isolated from	black liquor traetment system of cotton pulp mill
[Ref.: #8097]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #8097]	Country	China
[Ref.: #8097]	Country ISO 3 Code	CHN
[Ref.: #8097]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Black liquor treatment system of a cotton pulp mill
[Ref.: #67770]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Machines and devices
#Engineered	#Waste	#Industrial wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU566871 (>99% sequence identity) for Nesterenkonia alba subclade from Microbeatlas

Aquatic Samples	34
Soil Samples	21
Animal Samples	511
Plant Samples	15
Total Samples	581

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8097]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8097]	Nesterenkonia alba strain CAAS 252 16S ribosomal RNA gene, partial sequence	EU566871	1494	GenBank ENA	515814 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nesterenkonia alba DSM 19423	GCA_000421745	contig	GenBank ENA	1122602 tax ID
[Ref.: #66792]	Nesterenkonia alba DSM 19423	1122602.4	wgs	PATRIC	1122602 tax ID	*
[Ref.: #66792]	Nesterenkonia alba DSM 19423	2524023226	draft	JGI IMG/M	1122602 tax ID	*

[Ref.: #8097]	GC-content	60.2 mol%
[Ref.: #67770]	GC-content	63.7 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	60.2 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nesterenkonia alba DSM 19423 PATRIC: 1122602.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nesterenkonia alba DSM 19423 PATRIC: 1122602.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.926	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	93.449	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	79.512	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	79.398	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	91.96	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	89	yes

Availability in culture collections External links

[Ref.: #8097]

Culture collection no.

DSM 19423, CCTCC AB 207011, JCM 16549, CAAS 252, NBRC 107629

[Ref.: #77214]

SI-ID 407660

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica.	Anwar N, Ye Y, Chen C, Mamtimin T, Wang R, Rahman E, Wu M	Curr Microbiol	10.1007/s00284-021-02597-4	2021	*
Phylogeny	Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean.	Zhang G, Zhang Y, Yin X, Wang S	Int J Syst Evol Microbiol	10.1099/ijs.0.065623-0	2014	*
Phylogeny	Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill.	Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ, Fang CX, Yao B, Fan YL	Int J Syst Evol Microbiol	10.1099/ijs.0.003376-0	2009	*

References

#8097

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19423

#18336

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28995

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25428 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77214

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407660.1 )

#25428

IJSEM 863 2009 ( DOI 10.1099/ijs.0.003376-0 , PubMed 19329621 )

* These data were automatically processed and therefore are not curated

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