Nesterenkonia alba (DSM 19423, CCTCC AB 207011, JCM 16549)

Name: Nesterenkonia alba (DSM 19423, CCTCC AB 207011, JCM 16549)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7771

Type strain

Culture col. no. DSM 19423 CCTCC AB 207011 JCM 16549 CAAS 252 NBRC 107629

NCBI tax ID(s) 1122602 515814

Special Collections DSMZ JCM Wink compendium Literature strains

Links

Nesterenkonia alba DSM 19423 is a mesophilic, Gram-positive, rod-shaped prokaryote that was isolated from black liquor traetment system of cotton pulp mill.

Gram-positive rod-shaped mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407660

JCM 16549,DSM 19423

@ref 20215

Last LPSN update 2025-12-16 09:48:40

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Nesterenkonia

Species Nesterenkonia alba

Full scientific name Nesterenkonia alba Luo et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
28995	positive	1 µm	0.5 µm	rod-shaped

Colony morphology

@ref	Incubation period	Medium used
18336	10-14 days	ISP 4
18336	10-14 days	ISP 5
18336	10-14 days	ISP 6
18336	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18336		ISP 4
18336		ISP 5
18336		ISP 6
18336		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8097	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18336	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18336	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18336	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18336	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8097	positive	growth	37	mesophilic
18336	positive	optimum	37	mesophilic
28995	positive	growth	20-50
28995	positive	optimum	42	thermophilic
67770	positive	growth	37	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
28995	positive	optimum	9.5
28995	positive	growth	08-12	alkaliphile

Physiology and metabolism

Spore formation

28995

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
28995	NaCl	positive	growth	0-6 %
28995	NaCl	positive	optimum	3 %

Murein

@ref	Murein short key	Type
8097	A11.42	A4alpha L-Lys-Gly-D-Asp

Observation

@ref	Observation
28995	aggregates in clumps
67770	quinones: MK-8, MK-9, MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
28995	22599 ChEBI	arabinose	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
28995	5291 ChEBI	gelatin	+	carbon source
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
28995	17234 ChEBI	glucose	+	carbon source
28995	17754 ChEBI	glycerol	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
28995	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
28995	28053 ChEBI	melibiose	+	carbon source
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
28995	16634 ChEBI	raffinose	+	carbon source
28995	17992 ChEBI	sucrose	+	carbon source
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
28995	27082 ChEBI	trehalose	+	carbon source
28995	53426 ChEBI	tween 80	+	carbon source
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
28995	17151 ChEBI	xylitol	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
28995	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18336	-	+	+	-	-	+	-	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18336	not determinedn.d.	-	+	+	-	+	-	-	-	-	+	-	+	+	+	-	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Machines and devices
#Engineered	#Waste	#Industrial wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8097	black liquor traetment system of cotton pulp mill	Wuhan	China	CHN	Asia
67770	Black liquor treatment system of a cotton pulp mill	Wuhan	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence EU566871 (>99% sequence identity) for Nesterenkonia alba subclade from Microbeatlas

Aquatic Samples	34
Soil Samples	21
Animal Samples	511
Plant Samples	15
Total Samples	581

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8097	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM42174v1 assembly for Nesterenkonia alba DSM 19423	contig	GCA_000421745	1122602.4	2524023226	1122602	71.25

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8097	Nesterenkonia alba strain CAAS 252 16S ribosomal RNA gene, partial sequence	EU566871	1494		515814

GC content

@ref	GC-content (mol%)	Method
8097	60.2
67770	63.7	genome sequence analysis
67770	60.2	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Nesterenkonia alba DSM 19423
NCBI: GCA_000421745

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	88.50	no
125439	motility	BacteriaNetⓘ	no	73.40	no
125439	gram_stain	BacteriaNetⓘ	positive	86.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	73.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nesterenkonia alba DSM 19423
PATRIC: 1122602.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.93	yes
125438	anaerobic	anaerobicⓘ	no	93.45	no
125438	spore-forming	spore-formingⓘ	no	79.51	no
125438	aerobic	aerobicⓘ	yes	79.40	no
125438	thermophilic	thermophileⓘ	no	91.96	yes
125438	flagellated	motile2+ⓘ	no	89.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	The Osmoprotectant Switch of Potassium to Compatible Solutes in an Extremely Halophilic Archaea Halorubrum kocurii 2020YC7.	Ding R, Yang N, Liu J.	Genes (Basel)	10.3390/genes13060939	2022
Metabolism	Nesterenkonia sp. strain F, a halophilic bacterium producing acetone, butanol, and ethanol under aerobic conditions.	Amiri H, Azarbaijani R, Parsa Yeganeh L, Shahzadeh Fazeli A, Tabatabaei M, Salekdeh GH, Karimi K.	Sci Rep	10.1038/srep18408	2016
Genetics	Comparative Genomic Analyses of the Genus Nesterenkonia Unravels the Genomic Adaptation to Polar Extreme Environments.	Dai D, Lu H, Xing P, Wu Q.	Microorganisms	10.3390/microorganisms10020233	2022
	Novel Haloalkaliphilic Nitrile-Degrading Bacteria From Soda Lake Soil of Rift Valley Kenya.	Akinyi MD, Mwirichia R, Mugweru J, Kimani NM.	ScientificWorldJournal	10.1155/tswj/3126129	2025
Genetics	Comparative Genome Analysis of a Novel Alkaliphilic Actinobacterial Species Nesterenkonia haasae.	Wang S, Sun L, Narsing Rao MP, Fang BZ, Li WJ.	Pol J Microbiol	10.33073/pjm-2022-040	2022
	An insight into the utilization of microbial biosurfactants pertaining to their industrial applications in the food sector.	Venkataraman S, Rajendran DS, Vaidyanathan VK.	Food Sci Biotechnol	10.1007/s10068-023-01435-6	2024
	Production of Lipopeptide Biosurfactant by a Marine Nesterenkonia sp. and Its Application in Food Industry.	Kiran GS, Priyadharsini S, Sajayan A, Priyadharsini GB, Poulose N, Selvin J.	Front Microbiol	10.3389/fmicb.2017.01138	2017
Metabolism	Novel marine actinobacteria from emerald Andaman & Nicobar Islands: a prospective source for industrial and pharmaceutical byproducts.	Meena B, Rajan LA, Vinithkumar NV, Kirubagaran R.	BMC Microbiol	10.1186/1471-2180-13-145	2013
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Genetics	Genome sequence and description of Nesterenkonia massiliensis sp. nov. strain NP1(T.).	Edouard S, Sankar S, Dangui NP, Lagier JC, Michelle C, Raoult D, Fournier PE.	Stand Genomic Sci	10.4056/sigs.5631022	2014
Phylogeny	Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica.	Anwar N, Ye Y, Chen C, Mamtimin T, Wang R, Rahman E, Wu M	Curr Microbiol	10.1007/s00284-021-02597-4	2021
Phylogeny	Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean.	Zhang G, Zhang Y, Yin X, Wang S	Int J Syst Evol Microbiol	10.1099/ijs.0.065623-0	2014
Phylogeny	Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill.	Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ, Fang CX, Yao B, Fan YL	Int J Syst Evol Microbiol	10.1099/ijs.0.003376-0	2009

References

#8097	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19423
#18336	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28995	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25428 (see below)
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nesterenkonia alba (DSM 19423, CCTCC AB 207011, JCM 16549)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Spore formation

Halophily

Murein

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000421745

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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