Strain identifier

BacDive ID: 7771

Type strain: Yes

Species: Nesterenkonia alba

Strain history: DSM 19423 <-- B. Yao <-- B. Yao and H. Luo CAAS 252.

NCBI tax ID(s): 1122602 (strain), 515814 (species)

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General

@ref: 8097

BacDive-ID: 7771

DSM-Number: 19423

keywords: genome sequence, 16S sequence, Bacteria, Gram-positive, rod-shaped

description: Nesterenkonia alba DSM 19423 is a Gram-positive, rod-shaped bacterium that was isolated from black liquor traetment system of cotton pulp mill.

NCBI tax id

NCBI tax idMatching level
1122602strain
515814species

strain history

@refhistory
8097<- B. Yao <- B. Yao and H. Luo, Chinese Academy of Agriculture Sci., Feed Res. Institute, Beijing, China; CAAS 252
67770DSM 19423 <-- B. Yao <-- B. Yao and H. Luo CAAS 252.

doi: 10.13145/bacdive7771.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Nesterenkonia
  • species: Nesterenkonia alba
  • full scientific name: Nesterenkonia alba Luo et al. 2009

@ref: 8097

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Nesterenkonia

species: Nesterenkonia alba

full scientific name: Nesterenkonia alba Luo et al. 2009 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 28995
  • gram stain: positive
  • cell length: 1 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation periodmedium used
1833610-14 daysISP 4
1833610-14 daysISP 5
1833610-14 daysISP 6
1833610-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
18336noISP 4
18336noISP 5
18336noISP 6
18336noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8097TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18336ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18336ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18336ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18336ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
8097positivegrowth37
18336positiveoptimum37
28995positivegrowth20-50
28995positiveoptimum42
67770positivegrowth37

culture pH

@refabilitytypepHPH range
28995positivegrowth08-12alkaliphile
28995positiveoptimum9.5

Physiology and metabolism

spore formation

@refspore formationconfidence
28995no
69481no100

halophily

@refsaltgrowthtested relationconcentration
28995NaClpositivegrowth0-6 %
28995NaClpositiveoptimum3 %

murein

  • @ref: 8097
  • murein short key: A11.42
  • type: A4alpha L-Lys-Gly-D-Asp

observation

@refobservation
28995aggregates in clumps
67770quinones: MK-8, MK-9, MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2899522599arabinose+carbon source
289955291gelatin+carbon source
2899517234glucose+carbon source
2899517754glycerol+carbon source
2899517306maltose+carbon source
2899528053melibiose+carbon source
2899516634raffinose+carbon source
2899517992sucrose+carbon source
2899527082trehalose+carbon source
2899553426tween 80+carbon source
2899517151xylitol+carbon source
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
28995catalase+1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18336-++--+---+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18336-++-+----+-+++---+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8097black liquor traetment system of cotton pulp millWuhanChinaCHNAsia
67770Black liquor treatment system of a cotton pulp millWuhanChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Machines and devices
#Engineered#Waste#Industrial wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_4391.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2198;97_2660;98_3297;99_4391&stattab=map
  • Last taxonomy: Nesterenkonia alba subclade
  • 16S sequence: EU566871
  • Sequence Identity:
  • Total samples: 581
  • soil counts: 21
  • aquatic counts: 34
  • animal counts: 511
  • plant counts: 15

Safety information

risk assessment

  • @ref: 8097
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8097
  • description: Nesterenkonia alba strain CAAS 252 16S ribosomal RNA gene, partial sequence
  • accession: EU566871
  • length: 1494
  • database: nuccore
  • NCBI tax ID: 515814

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nesterenkonia alba DSM 194231122602.4wgspatric1122602
66792Nesterenkonia alba DSM 194232524023226draftimg1122602
67770Nesterenkonia alba DSM 19423GCA_000421745contigncbi1122602

GC content

@refGC-contentmethod
809760.2
6777063.7genome sequence analysis
6777060.2thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.926yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.449no
69480spore-formingspore-formingAbility to form endo- or exosporesno79.512no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes79.398no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno91.96yes
69480flagellatedmotile2+Ability to perform flagellated movementno89yes

External links

@ref: 8097

culture collection no.: DSM 19423, CCTCC AB 207011, JCM 16549, CAAS 252, NBRC 107629

straininfo link

  • @ref: 77214
  • straininfo: 407660

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19329621Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill.Luo HY, Wang YR, Miao LH, Yang PL, Shi PJ, Fang CX, Yao B, Fan YLInt J Syst Evol Microbiol10.1099/ijs.0.003376-02009Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Hydrogen-Ion Concentration, Micrococcaceae/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Temperature, Vitamin K 2/analysisGenetics
Phylogeny25389152Nesterenkonia alkaliphila sp. nov., an alkaliphilic, halotolerant actinobacteria isolated from the western Pacific Ocean.Zhang G, Zhang Y, Yin X, Wang SInt J Syst Evol Microbiol10.1099/ijs.0.065623-02014Actinobacteria/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, Micrococcaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Pacific Ocean, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny34468853Nesterenkonia ebinurensis sp. nov., a Novel Actinobacterium Isolated From Populus euphratica.Anwar N, Ye Y, Chen C, Mamtimin T, Wang R, Rahman E, Wu MCurr Microbiol10.1007/s00284-021-02597-42021Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Micrococcaceae, Nucleic Acid Hybridization, Peptidoglycan, Phospholipids/analysis, Phylogeny, *Populus, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
8097Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19423)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19423
18336Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM19423.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28995Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2542828776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77214Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID407660.1StrainInfo: A central database for resolving microbial strain identifiers