Name: Nesterenkonia flava DSM 19422
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7770

Type strain:

Species: Nesterenkonia flava

Culture col. no.: DSM 19422, CCTCC AB 207010, JCM 14814, CCUG 58060, CAAS 251, NBRC 107628

Strain history: B. Yao CAAS 251.

NCBI tax ID(s): 469799 (species)

SI-ID 405618

DSM 19422

Section

Nesterenkonia flava DSM 19422 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from paper mill effluent.

Gram-positive
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Nesterenkonia*
	Species	**Nesterenkonia flava**
	Full Scientific Name (LPSN)	Nesterenkonia flava Luo et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32520]	Gram stain	positive

[Ref.: #32520]	Cell length	1.2-1.4 µm

[Ref.: #32520]	Cell width	0.6-0.8 µm

[Ref.: #32520]	Cell shape	rod-shaped

[Ref.: #32520]	Motility	no

[Ref.: #19783]	Colony color	Beige (1001)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 2

[Ref.: #19783]	Colony color	Beige (1001)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 3

[Ref.: #19783]	Colony color	Traffic yellow (1023)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 4

[Ref.: #19783]	Colony color	Traffic yellow (1023)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 5

[Ref.: #19783]	Colony color	Traffic yellow (1023)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 6

[Ref.: #19783]	Colony color	Traffic yellow (1023)
[Ref.: #19783]	Incubation period	10-14 days
[Ref.: #19783]	Cultivation medium used	ISP 7

[Ref.: #32520]

Pigment production

yes

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 2

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 3

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 4

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 5

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 6

[Ref.: #19783]	Multicellular complex forming ability	no
[Ref.: #19783]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8096]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8096]

Culture medium growth

[Ref.: #8096]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8096]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 2

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 3

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 4

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 5

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 6

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

[Ref.: #19783]

Culture medium

ISP 7

[Ref.: #19783]

Culture medium growth

[Ref.: #19783]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8096]	growth	37 °C
[Ref.: #19783]	optimum	37 °C
[Ref.: #32520]	growth	10-50 °C
[Ref.: #32520]	optimum	40-42 °C
[Ref.: #61581]	growth	37 °C
[Ref.: #67770]	growth	40 °C

[Ref.: #8096]	Temperature range	mesophilic
[Ref.: #19783]	Temperature range	mesophilic
[Ref.: #32520]	Temperature range	thermophilic
[Ref.: #61581]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #32520]			optimum	09-10
[Ref.: #32520]			growth	08-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32520]	Oxygen tolerance	aerobe
[Ref.: #61581]	Oxygen tolerance	aerobe

[Ref.: #32520]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32520]		NaCl		growth	0-10	%

[Ref.: #8096]	Murein short key	A11.42
[Ref.: #8096]	Murein types	A4alpha L-Lys-Gly-D-Asp

[Ref.: #32520]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-8, MK-9, MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32520]	arabinose ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #32520]	fructose ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #32520]	glucose ChEBI	+	carbon source
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #32520]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #32520]	mannose ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #32520]	sucrose ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #32520]	catalase	+	1.11.1.6
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #19783]	320	-	+	-	-	-	+	+	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
[Ref.: #61581]	1766	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19783]	607	not determinedn.d.	-	+	+	-	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-
[Ref.: #61581]	2131	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8096]	Sample type/isolated from	paper mill effluent
[Ref.: #8096]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #8096]	Country	China
[Ref.: #8096]	Country ISO 3 Code	CHN
[Ref.: #8096]	Continent	Asia

[Ref.: #61581]	Sample type/isolated from	Paper-mill effluent
[Ref.: #61581]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #61581]	Country	China
[Ref.: #61581]	Country ISO 3 Code	CHN
[Ref.: #61581]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Paper-mill effluent
[Ref.: #67770]	Geographic location (country and/or sea, region)	Wuhan
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Engineered product
#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Waste	#Industrial wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF680886 (>99% sequence identity) for Nesterenkonia flava subclade from Microbeatlas

Aquatic Samples	46
Soil Samples	60
Animal Samples	397
Plant Samples	16
Total Samples	519

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8096]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19783]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8096]	Nesterenkonia flava strain CAAS 251 16S ribosomal RNA gene, partial sequence	EF680886	1412	ENA	469799 tax ID

[Ref.: #8096]	GC-content	65.5 mol%
[Ref.: #67770]	GC-content	65.5 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #8096]

Culture collection no.

DSM 19422, CCTCC AB 207010, JCM 14814, CCUG 58060, CAAS 251, NBRC 107628

[Ref.: #77213]

SI-ID 405618

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.	Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000917	2016	*
Phylogeny	Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert.	Wang HF, Zhang YG, Chen JY, Hozzein WN, Li L, Wadaan MAM, Zhang YM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.066894-0	2014	*
Phylogeny	Nesterenkonia flava sp. nov., isolated from paper-mill effluent.	Luo HY, Miao LH, Fang C, Yang PL, Wang YR, Shi PJ, Yao B, Fan YL	Int J Syst Evol Microbiol	10.1099/ijs.0.65618-0	2008	*

References

#8096

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19422

#19783

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32520

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28739 (see below)

#61581 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58060
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#77213

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405618.1 )

#28739

IJSEM 1927 2008 ( DOI 10.1099/ijs.0.65618-0 , PubMed 18676482 )

* These data were automatically processed and therefore are not curated

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