Nesterenkonia flava (DSM 19422, CCTCC AB 207010, JCM 14814)

Name: Nesterenkonia flava (DSM 19422, CCTCC AB 207010, JCM 14814)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7770

Type strain

Culture col. no. DSM 19422 CCTCC AB 207010 JCM 14814 CCUG 58060 CAAS 251 1 more

NCBI tax ID(s) 469799

Special Collections DSMZ CCUG JCM Wink compendium Literature strains

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Nesterenkonia flava DSM 19422 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from paper mill effluent.

Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405618

JCM 14814,NBRC 107628,DSM 19422,CCUG 58060T

@ref 20215

Last LPSN update 2025-12-16 09:48:40

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Micrococcaceae

Genus Nesterenkonia

Species Nesterenkonia flava

Full scientific name Nesterenkonia flava Luo et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
32520	positive	1.2-1.4 µm	0.6-0.8 µm	rod-shaped

Colony morphology

@ref	Colony color	Incubation period	Medium used
19783	Beige (1001)	10-14 days	ISP 2
19783	Beige (1001)	10-14 days	ISP 3
19783	Traffic yellow (1023)	10-14 days	ISP 4
19783	Traffic yellow (1023)	10-14 days	ISP 5
19783	Traffic yellow (1023)	10-14 days	ISP 6
19783	Traffic yellow (1023)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19783		ISP 2
19783		ISP 3
19783		ISP 4
19783		ISP 5
19783		ISP 6
19783		ISP 7

Pigmentation

32520

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8096	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
19783	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19783	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19783	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19783	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19783	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19783	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8096	positive	growth	37	mesophilic
19783	positive	optimum	37	mesophilic
32520	positive	growth	10-50
32520	positive	optimum	40-42	thermophilic
61581	positive	growth	37	mesophilic
67770	positive	growth	40	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
32520	positive	optimum	09-10
32520	positive	growth	08-12	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
32520	aerobe
61581	aerobe

Spore formation

32520

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
32520	NaCl	positive	growth	0-10 %

Murein

@ref	Murein short key	Type
8096	A11.42	A4alpha L-Lys-Gly-D-Asp

Observation

@ref	Observation
32520	aggregates in chains
67770	quinones: MK-8, MK-9, MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
32520	22599 ChEBI	arabinose	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
32520	28757 ChEBI	fructose	+	carbon source
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
32520	17234 ChEBI	glucose	+	carbon source
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
32520	17306 ChEBI	maltose	+	carbon source
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
32520	37684 ChEBI	mannose	+	carbon source
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
32520	17992 ChEBI	sucrose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
32520	catalase	+	1.11.1.6
68379	catalase	+	1.11.1.6	from API Coryne
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19783	-	+	-	-	-	+	+	-	-	+	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
61581	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19783	not determinedn.d.	-	+	+	-	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-
61581	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product
#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Waste	#Industrial wastewater

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
8096	paper mill effluent	Wuhan	China	CHN	Asia
61581	Paper-mill effluent	Wuhan	China	CHN	Asia
67770	Paper-mill effluent	Wuhan	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence EF680886 (>99% sequence identity) for Nesterenkonia flava subclade from Microbeatlas

Aquatic Samples	46
Soil Samples	60
Animal Samples	397
Plant Samples	16
Total Samples	519

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8096	1	Risk group (German classification) According to TRBA 466
19783	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM3143233v1 assembly for Nesterenkonia flava CCTCC AB 207010	scaffold	GCA_031432335	469799.3	469799	47.5
124043	ASM3953012v1 assembly for Nesterenkonia flava JCM 14814	contig	GCA_039530125		469799	40.46

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8096	Nesterenkonia flava strain CAAS 251 16S ribosomal RNA gene, partial sequence	EF680886	1412		469799

GC content

@ref	GC-content (mol%)	Method
8096	65.5
67770	65.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Metabolomic Investigations on Nesterenkonia flava Revealed Significant Differences between Marine and Terrestrial Actinomycetes.	Xie CL, Xia JM, Wang JS, Lin DH, Yang XW.	Mar Drugs	10.3390/md16100356	2018
Metabolism	Anti-Allergic Compounds from the Deep-Sea-Derived Actinomycete Nesterenkonia flava MCCC 1K00610.	Xie CL, Liu Q, Xia JM, Gao Y, Yang Q, Shao ZZ, Liu G, Yang XW.	Mar Drugs	10.3390/md15030071	2017
	Characterization and identification of charcoal of inedible Kerandang fish (Channa pleurophthalmus Blkr) body parts and potential antiallergenic properties.	Aryani A, Suprayitno E, Sasmito BB, Hardoko H.	Vet World	10.14202/vetworld.2020.1480-1486	2020
	Phylogenetic analysis of microbial communities in different regions of the gastrointestinal tract in Panaque nigrolineatus, a wood-eating fish.	McDonald R, Schreier HJ, Watts JE.	PLoS One	10.1371/journal.pone.0048018	2012
Phylogeny	Nesterenkonia cremea sp. nov., a bacterium isolated from a soda lake.	Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VR.	Int J Syst Evol Microbiol	10.1099/ijsem.0.001876	2017
Phylogeny	Nesterenkonia suensis sp. nov., a haloalkaliphilic actinobacterium isolated from a salt pan.	Govender L, Naidoo L, Setati ME.	Int J Syst Evol Microbiol	10.1099/ijs.0.035006-0	2013
Phylogeny	Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica.	Finore I, Orlando P, Di Donato P, Leone L, Nicolaus B, Poli A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000917	2016
Phylogeny	Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert.	Wang HF, Zhang YG, Chen JY, Hozzein WN, Li L, Wadaan MAM, Zhang YM, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.066894-0	2014
Phylogeny	Nesterenkonia flava sp. nov., isolated from paper-mill effluent.	Luo HY, Miao LH, Fang C, Yang PL, Wang YR, Shi PJ, Yao B, Fan YL	Int J Syst Evol Microbiol	10.1099/ijs.0.65618-0	2008

References

#8096	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19422
#19783	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#32520	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28739 (see below)
#61581	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58060
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nesterenkonia flava (DSM 19422, CCTCC AB 207010, JCM 14814)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Observation

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_031432335

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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