Name: Arthrobacter cryoconiti Cr6-08
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7629

Type strain:

Species: Arthrobacter cryoconiti

Strain Designation: Cr6-08

Culture col. no.: DSM 23324, CGMCC 1.10698, CIP 110638, LMG 26052, JCM 18953

Strain history: LMG 26052 <-- R. Margesin Cr6-08.

NCBI tax ID(s): 748907 (species)

Section

Arthrobacter cryoconiti Cr6-08 is an aerobe, psychrophilic, Gram-positive bacterium that was isolated from alpine glacier cryoconite.

Gram-positive
ovoid-shaped
aerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Arthrobacter*
	Species	**Arthrobacter cryoconiti**
	Full Scientific Name (PNU)	Arthrobacter cryoconiti Margesin et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30223]	Gram stain	positive
[Ref.: #30223]	Cell length	0.8-1.8 µm
[Ref.: #30223]	Cell width	0.5-0.7 µm
[Ref.: #30223]	Cell shape	ovoid-shaped
[Ref.: #30223]	Motility	no

[Ref.: #69303]	Colony color	Lemon yellow (1012)
[Ref.: #69303]	Cultivation medium used	ISP 7

[Ref.: #69303]	Colony color	Lemon yellow (1012)
[Ref.: #69303]	Cultivation medium used	suter with tyrosine

[Ref.: #69303]	Colony color	Lemon yellow (1012)
[Ref.: #69303]	Cultivation medium used	suter without tyrosine

[Ref.: #69303]	Colony color	Traffic yellow (1023)
[Ref.: #69303]	Cultivation medium used	ISP 3

[Ref.: #69303]	Colony color	Zinc yellow (1018)
[Ref.: #69303]	Cultivation medium used	ISP 2

[Ref.: #69303]	Colony color	Zinc yellow (1018)
[Ref.: #69303]	Cultivation medium used	ISP 5

[Ref.: #69303]	Colony color	Zinc yellow (1018)
[Ref.: #69303]	Cultivation medium used	ISP 6

[Ref.: #30223]	Pigment production	yes
[Ref.: #69303]	Pigment production	no
[Ref.: #69303]	Pigment name	Melanin
[Ref.: #69303]	Pigment production	no
[Ref.: #69303]	Pigment name	soluble pigment

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	ISP 2

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	ISP 3

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	ISP 5

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	ISP 6

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	ISP 7

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69303]	Multicellular complex forming ability	no
[Ref.: #69303]	Multicellular complex name	Aerial mycelium
[Ref.: #69303]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69303]

Caption:

Plates (830, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69303]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69303]

Caption:

Plates (830, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69303]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16555]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #16555]

Culture medium growth

[Ref.: #16555]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #16555]

Culture medium composition

expand / minimize

[Ref.: #36194]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #36194]

Culture medium growth

[Ref.: #36194]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #16555]	growth	20 °C
[Ref.: #30223]	growth	1-25 °C
[Ref.: #36194]	growth	20 °C
[Ref.: #67770]	growth	22 °C

[Ref.: #16555]	Temperature range	psychrophilic
[Ref.: #36194]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic

	pH	Kind of pH		pH	pH range
[Ref.: #30223]			growth	06-08

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30223]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30223]		NaCl		growth	0-3	%
[Ref.: #69303]		NaCl		growth	0-2.5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69303]	arabinose ChEBI	+/-	growth
[Ref.: #69303]	cellulose ChEBI	+/-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30223]	esculin ChEBI	+	hydrolysis
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69303]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #30223]	glucose ChEBI	+	carbon source
[Ref.: #69303]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69303]	inositol ChEBI	-	growth
[Ref.: #30223]	lactose ChEBI	+	carbon source
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30223]	malate ChEBI	+	carbon source
[Ref.: #30223]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #30223]	mannitol ChEBI	+	carbon source
[Ref.: #30223]	mannose ChEBI	+	carbon source
[Ref.: #69303]	mannose ChEBI	+	growth
[Ref.: #30223]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #30223]	phenylacetate ChEBI	+	carbon source
[Ref.: #69303]	raffinose ChEBI	+	growth
[Ref.: #69303]	rhamnose ChEBI	+	growth
[Ref.: #30223]	sodium citrate ChEBI	+	carbon source
[Ref.: #69303]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69303]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #30223]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #30223]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #30223]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69303]	3796	+	-	-	-	-	+	+	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69303]	5980	not determinedn.d.	-	+	+	+/-	+	+	+/-	-	-	+/-	+/-	+	+	-	+	-	+/-	+/-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16555]	Sample type/isolated from	alpine glacier cryoconite
[Ref.: #16555]	Geographic location (country and/or sea, region)	Ötztaler Alps, Banker glacier, 2820 m above sea level
[Ref.: #16555]	Country	Austria
[Ref.: #16555]	Country ISO 3 Code	AUT
[Ref.: #16555]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Banker glacier at an altitude of 2,820 m
[Ref.: #67770]	Geographic location (country and/or sea, region)	Ötztal Alps
[Ref.: #67770]	Country	Austria
[Ref.: #67770]	Country ISO 3 Code	AUT
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Glacier
#Condition	#Psychrophilic (<10°C)	-
#Climate	#Cold	#Alpine
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16555]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16555]	Arthrobacter cryoconiti strain Cr6-08 16S ribosomal RNA gene, partial sequence	GU784867	1526	ENA	748907 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Arthrobacter cryoconiti Cr6-08	GCA_020905375	contig	GenBank	748907 tax ID	*
[Ref.: #66792]	Candidatus Cryptobacteroides merdipullorum ChiHecec2B26-709	GCA_018715685	contig	PATRIC	2840771 tax ID	*

[Ref.: #16555]	GC-content	57.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #30223]	GC-content	57.3 mol%

Availability in culture collections External links

[Ref.: #16555]

Culture collection no.

DSM 23324, CGMCC 1.10698, CIP 110638, LMG 26052, JCM 18953

[Ref.: #20218]

Associated Passport(s) in StrainInfo

869062, 869061, 856270

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite.	Margesin R, Schumann P, Zhang DC, Redzic M, Zhou YG, Liu HC, Schinner F	Int J Syst Evol Microbiol	10.1099/ijs.0.031138-0	2011	*
Phylogeny	Arthrobacter dokdonellae sp. nov., isolated from a plant of the genus Campanula.	Koh HW, Kang MS, Lee KE, Lee EY, Kim H, Park SJ	J Microbiol	10.1007/s12275-019-8540-x	2019	*

References

#16555

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23324

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#30223

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26566 (see below)

#36194 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69303

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#26566

IJSEM 397 2012 ( DOI 10.1099/ijs.0.031138-0 , PubMed 21441372 )

* These data were automatically processed and therefore are not curated

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