Strain identifier

BacDive ID: 7629

Type strain: Yes

Species: Arthrobacter cryoconiti

Strain Designation: Cr6-08

Strain history: CIP <- 2013, DSMZ <- R. Margesin, Innsbruck Univ., Innsbruck, Austria: strain Cr6-08

NCBI tax ID(s): 748907 (species)

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General

@ref: 16555

BacDive-ID: 7629

DSM-Number: 23324

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-positive, ovoid-shaped

description: Arthrobacter cryoconiti Cr6-08 is an aerobe, psychrophilic, Gram-positive bacterium that was isolated from alpine glacier cryoconite.

NCBI tax id

  • NCBI tax id: 748907
  • Matching level: species

strain history

@refhistory
16555<- R. Margesin, Inst. Microbiology, Univ. Innsbruck, Austria; Cr6-08
67770LMG 26052 <-- R. Margesin Cr6-08.
118796CIP <- 2013, DSMZ <- R. Margesin, Innsbruck Univ., Innsbruck, Austria: strain Cr6-08

doi: 10.13145/bacdive7629.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Arthrobacter
  • species: Arthrobacter cryoconiti
  • full scientific name: Arthrobacter cryoconiti Margesin et al. 2012

@ref: 16555

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micrococcaceae

genus: Arthrobacter

species: Arthrobacter cryoconiti

full scientific name: Arthrobacter cryoconiti Margesin et al. 2012

strain designation: Cr6-08

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
30223positive0.8-1.8 µm0.5-0.7 µmovoid-shapedno
118796positiverod-shapedyes

colony morphology

@refcolony colormedium used
69303Lemon yellow (1012)ISP 7
69303Lemon yellow (1012)suter with tyrosine
69303Lemon yellow (1012)suter without tyrosine
69303Traffic yellow (1023)ISP 3
69303Zinc yellow (1018)ISP 2
69303Zinc yellow (1018)ISP 5
69303Zinc yellow (1018)ISP 6

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69303noAerial myceliumISP 2
69303noAerial myceliumISP 3
69303noAerial myceliumISP 5
69303noAerial myceliumISP 6
69303noAerial myceliumISP 7
69303noAerial myceliumsuter with tyrosine
69303noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
30223yes
69303noMelanin
69303nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
69303DSM_23324_image3.jpegPlates (830, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69303DSM_23324_image4.jpegPlates (830, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16555R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
36194MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
118796CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
16555positivegrowth20psychrophilic
30223positivegrowth1-25
36194positivegrowth20psychrophilic
67770positivegrowth22psychrophilic

culture pH

  • @ref: 30223
  • ability: positive
  • type: growth
  • pH: 06-08

Physiology and metabolism

oxygen tolerance

  • @ref: 30223
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
30223NaClpositivegrowth0-3 %
69303NaClpositivegrowth0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6930322599arabinose+/-growth
3022317234glucose+carbon source
3022317716lactose+carbon source
3022325115malate+carbon source
3022317306maltose+carbon source
3022329864mannitol+carbon source
3022337684mannose+carbon source
3022318401phenylacetate+carbon source
3022353258sodium citrate+carbon source
302234853esculin+hydrolysis
3022317632nitrate+reduction
6930362968cellulose+/-growth
6930328757fructose+growth
6930317234glucose+growth
6930317268inositol-growth
6930337684mannose+growth
6930316634raffinose+growth
6930326546rhamnose+growth
6930317992sucrose+growth
6930318222xylose-growth
6837917632nitrate+reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
30223acid phosphatase+3.1.3.2
30223alpha-galactosidase+3.2.1.22
30223catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69303+----++------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69303-+++/-+++/---+/-+/-++-+-+/-+/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16555alpine glacier cryoconiteÖtztaler Alps, Banker glacier, 2820 m above sea levelAustriaAUTEurope
67770Banker glacier at an altitude of 2,820 mÖtztal AlpsAustriaAUTEurope
118796Alpine glacier cryoconite, Banker glacierÖtzlaler Alps, AustriaAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Glacier
#Condition#Psychrophilic (<10°C)
#Climate#Cold#Alpine
#Environmental#Terrestrial#Geologic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
165551Risk group (German classification)
1187961Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16555
  • description: Arthrobacter cryoconiti strain Cr6-08 16S ribosomal RNA gene, partial sequence
  • accession: GU784867
  • length: 1526
  • database: ena
  • NCBI tax ID: 748907

Genome sequences

  • @ref: 66792
  • description: Arthrobacter cryoconiti Cr6-08
  • accession: GCA_020905375
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 748907

GC content

@refGC-contentmethod
1655557.3thermal denaturation, midpoint method (Tm)
3022357.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno92.407yes
gram-positiveyes94.568yes
anaerobicno99.581no
aerobicyes91.666yes
halophileno82.518no
spore-formingno87.93no
motileyes58.456no
thermophileno98.958yes
glucose-utilyes87.031yes
glucose-fermentno81.385yes

External links

@ref: 16555

culture collection no.: DSM 23324, CGMCC 1.10698, CIP 110638, LMG 26052, JCM 18953

straininfo link

  • @ref: 77073
  • straininfo: 370921

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21441372Arthrobacter cryoconiti sp. nov., a psychrophilic bacterium isolated from alpine glacier cryoconite.Margesin R, Schumann P, Zhang DC, Redzic M, Zhou YG, Liu HC, Schinner FInt J Syst Evol Microbiol10.1099/ijs.0.031138-02011*Altitude, Arthrobacter/chemistry/*classification/genetics/*isolation & purification, Austria, Bacterial Typing Techniques, Base Composition, *Cold Temperature, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Ice Cover/*microbiology, Lipids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny31079331Arthrobacter dokdonellae sp. nov., isolated from a plant of the genus Campanula.Koh HW, Kang MS, Lee KE, Lee EY, Kim H, Park SJJ Microbiol10.1007/s12275-019-8540-x2019Arthrobacter/classification/genetics/*isolation & purification/metabolism, Base Composition, Campanulaceae/*microbiology, Cell Wall/genetics/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Peptidoglycan/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of KoreaMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16555Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23324)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23324
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30223Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2656628776041
36194Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30442
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69303Wink, J.https://cdn.dsmz.de/wink/DSM%2023324.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
77073Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID370921.1StrainInfo: A central database for resolving microbial strain identifiers
118796Curators of the CIPCollection of Institut Pasteur (CIP 110638)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110638