Name: Leifsonia psychrotolerans LI1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7347

Type strain:

Species: Leifsonia psychrotolerans

Strain Designation: LI1

Culture col. no.: DSM 22824, JCM 19551, NCCB 100313

Strain history: DSM 22824 <-- L. Ganzert LI1 <-- F. Bajerski.

NCBI tax ID(s): 670054 (species)

SI-ID 400893

DSM 22824

Section

Leifsonia psychrotolerans LI1 is a psychrophilic bacterium that was isolated from initial soil covered by a moss layer.

psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Leifsonia*
	Species	**Leifsonia psychrotolerans**
	Full Scientific Name (LPSN)	Leifsonia psychrotolerans Ganzert et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #69227]	Colony color	Rape yellow (1021)
[Ref.: #69227]	Cultivation medium used	ISP 6

[Ref.: #69227]	Colony color	Traffic yellow (1023)
[Ref.: #69227]	Cultivation medium used	ISP 2

[Ref.: #69227]	Colony color	Zinc yellow (1018)
[Ref.: #69227]	Cultivation medium used	ISP 4

[Ref.: #69227]	Colony color	Zinc yellow (1018)
[Ref.: #69227]	Cultivation medium used	ISP 5

[Ref.: #69227]	Colony color	Zinc yellow (1018)
[Ref.: #69227]	Cultivation medium used	ISP 7

[Ref.: #69227]	Colony color	Zinc yellow (1018)
[Ref.: #69227]	Cultivation medium used	suter with tyrosine

[Ref.: #69227]	Colony color	Zinc yellow (1018)
[Ref.: #69227]	Cultivation medium used	suter without tyrosine

[Ref.: #69227]	Pigment production	no
[Ref.: #69227]	Pigment name	Melanin
[Ref.: #69227]	Pigment production	no
[Ref.: #69227]	Pigment name	soluble pigment

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	ISP 2

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	ISP 4

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	ISP 5

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	ISP 6

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	ISP 7

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69227]	Multicellular complex forming ability	no
[Ref.: #69227]	Multicellular complex name	Aerial mycelium
[Ref.: #69227]	Medium Name (multicellularity)	suter without tyrosine

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16536]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #16536]

Culture medium growth

[Ref.: #16536]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16536]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16536]			growth	16 °C
[Ref.: #67770]			growth	16 °C

[Ref.: #16536]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69227]		NaCl		growth	0-5	%

[Ref.: #67770]

Observation

quinones: MK-10, MK-11

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69227]	arabinose ChEBI	+	growth
[Ref.: #69227]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69227]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69227]	glucose ChEBI	+/-	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69227]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69227]	mannose ChEBI	-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69227]	raffinose ChEBI	-	growth
[Ref.: #69227]	rhamnose ChEBI	+/-	growth
[Ref.: #69227]	sucrose ChEBI	+/-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69227]	xylose ChEBI	+/-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69227]	3721	-	-	-	-	-	-	+	-	+/-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69227]	5905	not determinedn.d.	+/-	+/-	+	+/-	+	+	+/-	+/-	-	+	+/-	-	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16536]	Sample type/isolated from	initial soil covered by a moss layer
[Ref.: #16536]	Geographic location (country and/or sea, region)	South Shetland Islands, Livingston Island
[Ref.: #16536]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Moss-covered soil from Livingston Island
[Ref.: #67770]	Geographic location (country and/or sea, region)	South Shetland Islands
[Ref.: #67770]	Country	Antarctica
[Ref.: #67770]	Country ISO 3 Code	ATA
[Ref.: #67770]	Continent	Antarctica
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Moss

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16536]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16536]	Leifsonia psychrotolerans strain LI1 16S ribosomal RNA gene, partial sequence	GQ406810	1357	ENA	670054 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leifsonia psychrotolerans LI1	GCA_013410665	contig	GenBank	670054 tax ID	*
[Ref.: #66792]	Leifsonia psychrotolerans DSM 22824	2856451187	draft	JGI IMG/M	670054 tax ID	*

[Ref.: #16536]

GC-content

64.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #16536]

Culture collection no.

DSM 22824, JCM 19551, NCCB 100313

[Ref.: #76797]

SI-ID 400893

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leifsonia flava sp. nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.	Cai Y, Tao WZ, Ma YJ, Cheng J, Zhang MY, Zhang YX	J Microbiol	10.1007/s12275-018-8061-z	2018	*
Phylogeny	Description of Conyzicola nivalis sp. nov., isolated from glacial snow, and emended description of the genus Conyzicola and Conyzicola lurida.	Gu Z, Liu Y, Xu B, Wang N, Shen L, Liu H, Zhou Y, Xing T, Guo B, Liu X	Int J Syst Evol Microbiol	10.1099/ijsem.0.002027	2017	*
Phylogeny	Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica.	Ganzert L, Bajerski F, Mangelsdorf K, Lipski A, Wagner D	Int J Syst Evol Microbiol	10.1099/ijs.0.021956-0	2010	*

References

#16536

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22824

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69227

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#76797

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400893.1 )

* These data were automatically processed and therefore are not curated

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