Strain identifier

BacDive ID: 7347

Type strain: Yes

Species: Leifsonia psychrotolerans

Strain Designation: LI1

Strain history: DSM 22824 <-- L. Ganzert LI1 <-- F. Bajerski.

NCBI tax ID(s): 670054 (species)

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General

@ref: 16536

BacDive-ID: 7347

DSM-Number: 22824

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic

description: Leifsonia psychrotolerans LI1 is a psychrophilic bacterium that was isolated from initial soil covered by a moss layer.

NCBI tax id

  • NCBI tax id: 670054
  • Matching level: species

strain history

@refhistory
16536<- L. Ganzert, Alfred Wegener Inst. Polar and Marine Res. in the Helmholtz Association, Res. Unit Potsdam, Germany; LI1 <- F. Bajerski
67770DSM 22824 <-- L. Ganzert LI1 <-- F. Bajerski.

doi: 10.13145/bacdive7347.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Leifsonia
  • species: Leifsonia psychrotolerans
  • full scientific name: Leifsonia psychrotolerans Ganzert et al. 2011

@ref: 16536

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Leifsonia

species: Leifsonia psychrotolerans

full scientific name: Leifsonia psychrotolerans Ganzert et al. 2011 emend. Cai et al. 2018

strain designation: LI1

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69227Rape yellow (1021)ISP 6
69227Traffic yellow (1023)ISP 2
69227Zinc yellow (1018)ISP 4
69227Zinc yellow (1018)ISP 5
69227Zinc yellow (1018)ISP 7
69227Zinc yellow (1018)suter with tyrosine
69227Zinc yellow (1018)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69227noAerial myceliumISP 2
69227noAerial myceliumISP 4
69227noAerial myceliumISP 5
69227noAerial myceliumISP 6
69227noAerial myceliumISP 7
69227noAerial myceliumsuter with tyrosine
69227noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
69227noMelanin
69227nosoluble pigment

Culture and growth conditions

culture medium

  • @ref: 16536
  • name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/92
  • composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16536positivegrowth16psychrophilic
67770positivegrowth16psychrophilic

Physiology and metabolism

halophily

  • @ref: 69227
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

observation

  • @ref: 67770
  • observation: quinones: MK-10, MK-11

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6922722599arabinose+growth
6922762968cellulose-growth
6922728757fructose+growth
6922717234glucose+/-growth
6922717268inositol-growth
6922737684mannose-growth
6922716634raffinose-growth
6922726546rhamnose+/-growth
6922717992sucrose+/-growth
6922718222xylose+/-growth
6837917632nitrate-reduction
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382esterase lipase (C 8)+
68379gelatinase-
68379urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69227------+-+/-----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69227+/-+/-++/-+++/-+/--++/--+-+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
16536initial soil covered by a moss layerSouth Shetland Islands, Livingston IslandAustralia and Oceania
67770Moss-covered soil from Livingston IslandSouth Shetland IslandsAntarcticaAntarcticaATA

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Moss

Safety information

risk assessment

  • @ref: 16536
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16536
  • description: Leifsonia psychrotolerans strain LI1 16S ribosomal RNA gene, partial sequence
  • accession: GQ406810
  • length: 1357
  • database: ena
  • NCBI tax ID: 670054

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leifsonia psychrotolerans LI1GCA_013410665contigncbi670054
66792Leifsonia psychrotolerans DSM 228242856451187draftimg670054

GC content

  • @ref: 16536
  • GC-content: 64.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 16536

culture collection no.: DSM 22824, JCM 19551, NCCB 100313

straininfo link

  • @ref: 76797
  • straininfo: 400893

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20833887Leifsonia psychrotolerans sp. nov., a psychrotolerant species of the family Microbacteriaceae from Livingston Island, Antarctica.Ganzert L, Bajerski F, Mangelsdorf K, Lipski A, Wagner DInt J Syst Evol Microbiol10.1099/ijs.0.021956-02010Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Antarctic Regions, Cold Temperature, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyMetabolism
Phylogeny28820090Description of Conyzicola nivalis sp. nov., isolated from glacial snow, and emended description of the genus Conyzicola and Conyzicola lurida.Gu Z, Liu Y, Xu B, Wang N, Shen L, Liu H, Zhou Y, Xing T, Guo B, Liu XInt J Syst Evol Microbiol10.1099/ijsem.0.0020272017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Ice Cover/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Snow/*microbiology, Vitamin K 2/chemistryTranscriptome
Phylogeny30047083Leifsonia flava sp. nov., a novel actinobacterium isolated from the rhizosphere of Aquilegia viridiflora.Cai Y, Tao WZ, Ma YJ, Cheng J, Zhang MY, Zhang YXJ Microbiol10.1007/s12275-018-8061-z2018Actinobacteria/*classification/genetics/*isolation & purification/physiology, Aquilegia/*growth & development, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, Pigments, Biological/analysis, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Sodium Chloride/metabolism, *Soil Microbiology, Temperature, Vitamin K 2/analysisTranscriptome

Reference

@idauthorscataloguedoi/urltitle
16536Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22824)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22824
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69227Wink, J.https://cdn.dsmz.de/wink/DSM%2022824.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
76797Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400893.1StrainInfo: A central database for resolving microbial strain identifiers