Legionella pneumophila subsp. pascullei (DSM 7515, ATCC 33737, CCUG 31226 A)

Name: Legionella pneumophila subsp. pascullei (DSM 7515, ATCC 33737, CCUG 31226 A)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6728

Type strain

Strain Designation U8W

Culture col. no. DSM 7515 ATCC 33737 CCUG 31226 A U8W CIP 105570

NCBI tax ID(s) 91890

Special Collections DSMZ CCUG CIP

Links

Legionella pneumophila subsp. pascullei U8W is a microaerophile, mesophilic, Gram-negative prokaryote that was isolated from water from shower head.

Gram-negative rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 40661

ATCC 33737,CCUG 31226 A,CIP 105570,DSM 7515,NCTC 12273

@ref 20215

Last LPSN update 2025-12-16 09:48:24

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella pneumophila subsp. pascullei

Full scientific name Legionella pneumophila subsp. pascullei Brenner et al. 1989

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121908	negative	rod-shaped
125439	negative

Colony morphology

3195

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3195	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40189	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121908	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3195	positive	growth	37	mesophilic
40189	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
3195	microaerophile
125439	aerobe

Spore formation

@ref	Spore formation	Confidence
125439		99

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
3195	catalase	+	1.11.1.6
121908	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
3195	cytochrome-c oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121908	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121908	-	+	+	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
121908	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3195	water from shower head	Pennsylvania, Pittsburgh	USA	USA	North America
50482	Water from shower head
121908	Environment, Water from shower head

Taxonmaps

69479

Global distribution of 16S sequence AF122885 (>99% sequence identity) for Legionella pneumophila subclade from Microbeatlas

Aquatic Samples	1091
Soil Samples	587
Animal Samples	330
Plant Samples	95
Total Samples	2103

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3195	2	Risk group (German classification) According to TRBA 466
121908	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ATCC_33737 assembly for Legionella pneumophila subsp. pascullei ATCC 33737	scaffold	GCA_000586255	91890.3	2671180510	91890	70.4

Genome browser: GCA_000586255

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Legionella pneumophila subsp. pascullei 16S ribosomal RNA gene, partial sequence	AF122885	1454	91890
20218	Legionella pneumophila subsp. pascullei strain MDC1574 16S ribosomal RNA gene, partial sequence	JF720379	524	91890
124043	Legionella pneumophila subsp. pascullei 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ile gene, complete sequence.	KM609987	397	91890
124043	Legionella pneumophila subsp. pascullei 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ala gene, complete sequence.	KM609986	382	91890

GC content

@ref	GC-content (mol%)	Method
3195	39.0	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Legionella pneumophila subsp. pascullei ATCC 33737
NCBI: GCA_000586255

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.00	no
125439	motility	BacteriaNetⓘ	yes	53.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	93.90	no

Literature

Title	Authors	Journal	DOI	Year
Impact of Chlorine Dioxide on Pathogenic Waterborne Microorganisms Occurring in Dental Chair Units.	Kruger TIM, Herzog S, Mellmann A, Kuczius T.	Microorganisms	10.3390/microorganisms11051123	2023
Comparative genome analysis reveals a complex population structure of Legionella pneumophila subspecies.	Kozak-Muiznieks NA, Morrison SS, Mercante JW, Ishaq MK, Johnson T, Caravas J, Lucas CE, Brown E, Raphael BH, Winchell JM.	Infect Genet Evol	10.1016/j.meegid.2018.02.008	2018
The N-acylneuraminate cytidyltransferase gene, neuA, is heterogenous in Legionella pneumophila strains but can be used as a marker for epidemiological typing in the consensus sequence-based typing scheme.	Farhat C, Mentasti M, Jacobs E, Fry NK, Luck C.	J Clin Microbiol	10.1128/jcm.00687-11	2011
Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Multilocus enzyme analysis of Legionella dumoffii.	Woods TC, McKinney RM, Plikaytis BD, Steigerwalt AG, Bibb WF, Brenner DJ.	J Clin Microbiol	10.1128/jcm.26.5.799-803.1988	1988
Genetic structure of populations of Legionella pneumophila.	Selander RK, McKinney RM, Whittam TS, Bibb WF, Brenner DJ, Nolte FS, Pattison PE.	J Bacteriol	10.1128/jb.163.3.1021-1037.1985	1985
Usefulness of fatty acid composition for differentiation of Legionella species.	Diogo A, Verissimo A, Nobre MF, da Costa MS.	J Clin Microbiol	10.1128/jcm.37.7.2248-2254.1999	1999
Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov.	Brenner DJ, Steigerwalt AG, Epple P, Bibb WF, McKinney RM, Starnes RW, Colville JM, Selander RK, Edelstein PH, Moss CW.	J Clin Microbiol	10.1128/jcm.26.9.1695-1703.1988	1988

References

#3195	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7515
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40189	; Curators of the CIP;
#50482	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31226 A
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121908	Collection of Institut Pasteur ; Curators of the CIP; CIP 105570
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella pneumophila subsp. pascullei (DSM 7515, ATCC 33737, CCUG 31226 A)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000586255

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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