Strain identifier

BacDive ID: 6728

Type strain: Yes

Species: Legionella pneumophila subsp. pascullei

Strain Designation: U8W

Strain history: <- ATCC <- A. Brown, U8W

NCBI tax ID(s): 91890 (subspecies)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3195

BacDive-ID: 6728

DSM-Number: 7515

keywords: microaerophile, Bacteria, 16S sequence, genome sequence, mesophilic

description: Legionella pneumophila subsp. pascullei U8W is a microaerophile, mesophilic Proteobacterium that was isolated from water from shower head.

NCBI tax id

  • NCBI tax id: 91890
  • Matching level: subspecies

strain history: <- ATCC <- A. Brown, U8W

doi: 10.13145/bacdive6728.20211221.6

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. pascullei
  • full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989

@ref: 3195

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. pascullei

full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989

strain designation: U8W

type strain: yes

Morphology

colony morphology

  • @ref: 3195
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3195BCYE-AGAR (DSMZ Medium 585)yeshttps://bacmedia.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40189MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)

culture temp

@refgrowthtypetemperaturerange
3195positivegrowth37mesophilic
40189positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3195
  • oxygen tolerance: microaerophile

enzymes

@refvalueactivityec
3195catalase+1.11.1.6
3195cytochrome-c oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3195water from shower headPennsylvania, PittsburghUSAUSANorth America
50482Water from shower head

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Engineered product
#Environmental#Aquatic

Safety information

risk assessment

  • @ref: 3195
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella pneumophila subsp. pascullei 16S ribosomal RNA gene, partial sequenceAF1228851454ena91890
20218Legionella pneumophila subsp. pascullei strain MDC1574 16S ribosomal RNA gene, partial sequenceJF720379524ena91890

Genome sequences

  • @ref: 66792
  • description: Legionella pneumophila pascullei ATCC 33737
  • accession: 2671180510
  • assembly level: draft
  • database: img
  • NCBI tax ID: 91890

GC content

  • @ref: 3195
  • GC-content: 39.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3195

culture collection no.: DSM 7515, ATCC 33737, CCUG 31226 A

straininfo link

@refpassport
20218http://www.straininfo.net/strains/152360
20218http://www.straininfo.net/strains/109710

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3195Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7515)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7515
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40189Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17604
50482Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31226 A)https://www.ccug.se/strain?id=31226
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)