Strain identifier

BacDive ID: 6728

Type strain: Yes

Species: Legionella pneumophila subsp. pascullei

Strain Designation: U8W

Strain history: CIP <- 1998, Benson, CDC <- A. Brown: strain U8W

NCBI tax ID(s): 91890 (subspecies)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3195

BacDive-ID: 6728

DSM-Number: 7515

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella pneumophila subsp. pascullei U8W is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water from shower head.

NCBI tax id

  • NCBI tax id: 91890
  • Matching level: subspecies

strain history

@refhistory
3195<- ATCC <- A. Brown, U8W
121908CIP <- 1998, Benson, CDC <- A. Brown: strain U8W

doi: 10.13145/bacdive6728.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella pneumophila subsp. pascullei
  • full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989

@ref: 3195

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella pneumophila subsp. pascullei

full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989

strain designation: U8W

type strain: yes

Morphology

cell morphology

  • @ref: 121908
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 3195
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3195BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40189MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
121908CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
3195positivegrowth37mesophilic
40189positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3195
  • oxygen tolerance: microaerophile

enzymes

@refvalueactivityec
3195catalase+1.11.1.6
3195cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
121908oxidase-
121908catalase+1.11.1.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121908-+++-+++--++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121908---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3195water from shower headPennsylvania, PittsburghUSAUSANorth America
50482Water from shower head
121908Environment, Water from shower head

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Engineered product
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_897.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_73;98_80;99_897&stattab=map
  • Last taxonomy: Legionella pneumophila subclade
  • 16S sequence: AF122885
  • Sequence Identity:
  • Total samples: 2103
  • soil counts: 587
  • aquatic counts: 1091
  • animal counts: 330
  • plant counts: 95

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
31952Risk group (German classification)
1219082Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella pneumophila subsp. pascullei 16S ribosomal RNA gene, partial sequenceAF1228851454ena91890
20218Legionella pneumophila subsp. pascullei strain MDC1574 16S ribosomal RNA gene, partial sequenceJF720379524ena91890

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella pneumophila pascullei ATCC 337372671180510draftimg91890
66792Legionella pneumophila subsp. pascullei ATCC 33737GCA_000586255scaffoldncbi91890

GC content

  • @ref: 3195
  • GC-content: 39.0
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 3195

culture collection no.: DSM 7515, ATCC 33737, CCUG 31226 A, CIP 105570

straininfo link

  • @ref: 76198
  • straininfo: 40661

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3195Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7515)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7515
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40189Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17604
50482Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31226 A)https://www.ccug.se/strain?id=31226
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76198Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40661.1StrainInfo: A central database for resolving microbial strain identifiers
121908Curators of the CIPCollection of Institut Pasteur (CIP 105570)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105570