Strain identifier
BacDive ID: 6728
Type strain:
Species: Legionella pneumophila subsp. pascullei
Strain Designation: U8W
Strain history: CIP <- 1998, Benson, CDC <- A. Brown: strain U8W
NCBI tax ID(s): 91890 (subspecies)
General
@ref: 3195
BacDive-ID: 6728
DSM-Number: 7515
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped
description: Legionella pneumophila subsp. pascullei U8W is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water from shower head.
NCBI tax id
- NCBI tax id: 91890
- Matching level: subspecies
strain history
@ref | history |
---|---|
3195 | <- ATCC <- A. Brown, U8W |
121908 | CIP <- 1998, Benson, CDC <- A. Brown: strain U8W |
doi: 10.13145/bacdive6728.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella pneumophila subsp. pascullei
- full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989
@ref: 3195
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella pneumophila subsp. pascullei
full scientific name: Legionella pneumophila subsp. pascullei Brenner et al. 1989
strain designation: U8W
type strain: yes
Morphology
cell morphology
- @ref: 121908
- gram stain: negative
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 3195
- incubation period: 2-3 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3195 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
40189 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
121908 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3195 | positive | growth | 37 | mesophilic |
40189 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 3195
- oxygen tolerance: microaerophile
enzymes
@ref | value | activity | ec |
---|---|---|---|
3195 | catalase | + | 1.11.1.6 |
3195 | cytochrome-c oxidase | + | 1.9.3.1 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | valine arylamidase | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
121908 | oxidase | - | |
121908 | catalase | + | 1.11.1.6 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121908 | - | + | + | + | - | + | + | + | - | - | + | + | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121908 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
3195 | water from shower head | Pennsylvania, Pittsburgh | USA | USA | North America |
50482 | Water from shower head | ||||
121908 | Environment, Water from shower head |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Engineered product |
#Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_897.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_73;98_80;99_897&stattab=map
- Last taxonomy: Legionella pneumophila subclade
- 16S sequence: AF122885
- Sequence Identity:
- Total samples: 2103
- soil counts: 587
- aquatic counts: 1091
- animal counts: 330
- plant counts: 95
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
3195 | 2 | Risk group (German classification) |
121908 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Legionella pneumophila subsp. pascullei 16S ribosomal RNA gene, partial sequence | AF122885 | 1454 | ena | 91890 |
20218 | Legionella pneumophila subsp. pascullei strain MDC1574 16S ribosomal RNA gene, partial sequence | JF720379 | 524 | ena | 91890 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Legionella pneumophila pascullei ATCC 33737 | 2671180510 | draft | img | 91890 |
66792 | Legionella pneumophila subsp. pascullei ATCC 33737 | GCA_000586255 | scaffold | ncbi | 91890 |
GC content
- @ref: 3195
- GC-content: 39.0
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 3195
culture collection no.: DSM 7515, ATCC 33737, CCUG 31226 A, CIP 105570
straininfo link
- @ref: 76198
- straininfo: 40661
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3195 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7515) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-7515 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40189 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17604 | ||||
50482 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 31226 A) | https://www.ccug.se/strain?id=31226 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
76198 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40661.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121908 | Curators of the CIP | Collection of Institut Pasteur (CIP 105570) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105570 |