Name: Legionella pneumophila subsp. fraseri R50
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6727

Type strain:

Species: Legionella pneumophila subsp. fraseri

Strain Designation: R50, Dallas 1 E SG5, Dallas 1E

Culture col. no.: DSM 25121, ATCC 33216, CCUG 13399, CECT 7274, CIP 103859, NCTC 11405

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.M. McKinney: strain Dallas 1E <- P. Skalyi

NCBI tax ID(s): 91892 (subspecies)

SI-ID 40396

ATCC 33216, CCUG 13399, NCTC 11405, CIP 103859, CECT 7274, DSM 25121

Other strains from Legionella pneumophila subsp. fraseri

L. pneumophila subsp. fraseri DSM 7514, ATCC 33156, WDCM 00180, ... (type strain)

Section

Legionella pneumophila subsp. fraseri R50 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from cooling tower.

Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella pneumophila subsp. fraseri**
	Full Scientific Name (LPSN)	Legionella pneumophila subsp. fraseri Brenner et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #121901]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #121901]	Cell shape	rod-shaped

[Ref.: #121901]	Motility	no

[Ref.: #17912]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17912]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #17912]

Culture medium growth

[Ref.: #17912]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17912]

Culture medium composition

expand / minimize

[Ref.: #38478]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #38478]

Culture medium growth

[Ref.: #38478]

Culture medium composition

expand / minimize

[Ref.: #121901]

Culture medium

CIP Medium 23

[Ref.: #121901]

Culture medium growth

[Ref.: #121901]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121901]

Culture medium

CIP Medium 748

[Ref.: #121901]

Culture medium growth

[Ref.: #121901]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #17912]	growth	37 °C
[Ref.: #38478]	growth	37 °C
[Ref.: #45944]	growth	37 °C
[Ref.: #121901]	growth	37 °C

[Ref.: #17912]	Temperature range	mesophilic
[Ref.: #38478]	Temperature range	mesophilic
[Ref.: #45944]	Temperature range	mesophilic
[Ref.: #121901]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17912]	Oxygen tolerance	microaerophile
[Ref.: #45944]	Oxygen tolerance	microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.987

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #17912]	catalase	+	1.11.1.6
[Ref.: #121901]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #17912]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121901]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #45944]

Fatty acid	Percentage	ECL
C16:0 iso	26.5	15.626
C15:0 ANTEISO	23.6	14.711
C16:1 ω7c	11.4	15.819
C14:0 ISO	10	13.618
C17:0 anteiso	5.6	16.722
C16:1 ISO H	4.6	15.461
C16:0	4	16
C15:1 ω6c	3.3	14.856
C17:0 CYCLO	3.1	16.888
C14:0 ISO 3OH	2.5	15.117
Unidentified	1.3
C14:1 ω5c	1.2	13.899
C18:0	1.1	18
C15:0	1	15
C17:1 ω9c ANTEISO	0.9	16.525

Metadata FA analysis

Reference

[Ref.: #45944]

Fatty acid	Percentage	ECL
C16:0 iso	23.7	15.626
C15:0 ANTEISO	21.6	14.711
C16:1 ω7c	18.9	15.819
C14:0 ISO	9.4	13.618
C16:0	4.9	16
C16:1 ISO H	4.5	15.461
C17:0 anteiso	4.2	16.722
C15:1 ω6c	3.8	14.856
C14:0 ISO 3OH	1.4	15.117
C15:0	1.3	15
C14:1 ω5c	1.2	13.899
C17:0 CYCLO	0.9	16.888
C17:1 ω9c ANTEISO	0.8	16.525
C14:0	0.8	14
Unidentified	0.8	16.313
C18:0	0.7	18
C15:0 ISO	0.5	14.621
C15:0 2OH	0.4	16.217
C17:0	0.3	17

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121901]	7707	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17912]	Sample type/isolated from	cooling tower

[Ref.: #45944]	Sample type/isolated from	Water from cooling tower
[Ref.: #45944]	Geographic location (country and/or sea, region)	Dallas
[Ref.: #45944]	Country	USA
[Ref.: #45944]	Country ISO 3 Code	USA
[Ref.: #45944]	Continent	North America

[Ref.: #121901]	Sample type/isolated from	Other, Cooling tower
[Ref.: #121901]	Country	United States of America
[Ref.: #121901]	Country ISO 3 Code	USA
[Ref.: #121901]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Cooling tower

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17912]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121901]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	L.pneumophila (strain Dallas-1E'' subspecies uncertain) 16S rRNA, partial, segment 2 of 3	M19447	135	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Dallas-1E'' subspecies uncertain) 16S rRNA, partial, segment 1 of 3	M19452	119	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Dallas-1E'' subspecies uncertain) 16S rRNA, partial, segment 3 of 3	M22265	107	ENA	446 tax ID	*
[Ref.: #20218]	Legionella pneumophila 16S rRNA sequence	M36026	1460	ENA	446 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella pneumophila ATCC 33216	GCA_001582695	scaffold	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. fraseri ATCC 33216	GCA_000586275	scaffold	GenBank	91892 tax ID	*
[Ref.: #66792]	Legionella pneumophila strain ATCC 33216	446.171	wgs	PATRIC	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila fraseri ATCC 33216	2660238543	draft	JGI IMG/M	91892 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella pneumophila ATCC 33216 NCBI: GCA_001582695.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella pneumophila ATCC 33216 NCBI: GCA_001582695.1
[Ref.: #69480]	flagellated	no	79.108	no
[Ref.: #69480]	gram-positive	no	98.428	no
[Ref.: #69480]	anaerobic	no	98.79	no
[Ref.: #69480]	aerobic	no	89.569	yes
[Ref.: #69480]	halophile	no	85.647	no
[Ref.: #69480]	spore-forming	no	94.795	no
[Ref.: #69480]	glucose-util	yes	69.656	no
[Ref.: #69480]	motile	yes	76.18	no
[Ref.: #69480]	thermophile	no	98.271	yes
[Ref.: #69480]	glucose-ferment	no	86.374	no

Availability in culture collections External links

[Ref.: #17912]

Culture collection no.

DSM 25121, ATCC 33216, CCUG 13399, CECT 7274, CIP 103859, NCTC 11405

[Ref.: #76197]

SI-ID 40396

References

#17912

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25121

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38478 ; Curators of the CIP;
#45944 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 13399

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76197

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40396.1 )

#121901 Collection of Institut Pasteur ; Curators of the CIP; CIP 103859
* These data were automatically processed and therefore are not curated

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Legionella pneumophila ATCC 33216

Legionella pneumophila ATCC 33216

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