Name: Legionella longbeachae CCUG 46622
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6729

Type strain:

Species: Legionella longbeachae

Culture col. no.: CCUG 46622, CIP 103880, DSM 10572, ATCC 33462, NCTC 11477, Long Beach 4, CCUG 13335

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.M. McKinney: strain Long Beach 4

NCBI tax ID(s): 450 (species)

SI-ID 40509

ATCC 33462, NCTC 11477, CIP 103880, CCUG 13335, DSM 10572, CCUG 46622

Special Collections

Other strains from Legionella longbeachae

Section

When using BacDive for research please cite our paper

Legionella longbeachae CCUG 46622 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung.

Gram-negative
motile
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella longbeachae**
	Full Scientific Name (LPSN)	Legionella longbeachae McKinney et al. 1982

Information on morphological and physiological properties Morphology

[Ref.: #120647]	Gram stain	negative
[Ref.: #125439]	Gram stain	negative Confidence in %97.5
[Ref.: #125438]	Gram stain	negative Confidence in %99

[Ref.: #120647]	Cell shape	rod-shaped

[Ref.: #120647]	Motility	yes

[Ref.: #3979]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3979]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #3979]

Culture medium growth

[Ref.: #3979]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3979]

Culture medium composition

expand / minimize

[Ref.: #40846]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40846]

Culture medium growth

[Ref.: #40846]

Culture medium composition

expand / minimize

[Ref.: #120647]

Culture medium

CIP Medium 23

[Ref.: #120647]

Culture medium growth

[Ref.: #120647]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3979]	growth	37 °C
[Ref.: #40846]	growth	37 °C
[Ref.: #57290]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3979]	Oxygen tolerance	microaerophile
[Ref.: #57290]	Oxygen tolerance	microaerophile
[Ref.: #120647]	Oxygen tolerance	facultative anaerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %97.9

[Ref.: #125439]	Ability of spore formation	no Confidence in %96.8
[Ref.: #125438]	Ability of spore formation	no Confidence in %91.967

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #3979]	catalase	+	1.11.1.6
[Ref.: #120647]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #3979]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120647]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #57290]

Fatty acid	Percentage	ECL
C16:1 ω7c	23.7	15.819
C16:0 iso	23.3	15.626
C15:0 ANTEISO	17.6	14.711
C17:0 CYCLO	10.2	16.888
C14:0 ISO	7.4	13.618
C17:0 anteiso	5.3	16.722
C16:0	4	16
C16:1 ω5c	2.7	15.908
C15:1 ω6c	2.2	14.856
C15:0	0.8	15
C17:0	0.6	17
C19:0 CYCLO ω9c	0.5	18.87
C14:1 ω5c	0.5	13.899
C17:0 iso	0.4	16.629
C14:0	0.4	14
C16:1 ISO H	0.3	15.461
C15:0 ISO	0.3	14.621

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120647]	7722	-	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3979]	Sample type/isolated from	human lung
[Ref.: #3979]	Geographic location (country and/or sea, region)	California
[Ref.: #3979]	Country	USA
[Ref.: #3979]	Country ISO 3 Code	USA
[Ref.: #3979]	Continent	North America

[Ref.: #57290]	Sample type/isolated from	Human lung

[Ref.: #120647]	Sample type/isolated from	Human, Lung
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Lung

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY444740 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	754
Soil Samples	787
Animal Samples	213
Plant Samples	102
Total Samples	1856

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3979]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120647]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Legionella longbeachae strain ATCC 33462 16S ribosomal RNA gene, partial sequence	AY444740	1466	GenBank ENA	450 tax ID	*
[Ref.: #20218]	Legionella longbeachae 16S rRNA sequence	M36029	1473	GenBank ENA	450 tax ID	*
[Ref.: #20218]	Legionella longbeachae strain MDC1575 16S ribosomal RNA gene, partial sequence	JF720398	524	GenBank ENA	450 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella longbeachae strain FDAARGOS_1481	450.92	complete	PATRIC	450 tax ID	*
[Ref.: #66792]	Legionella longbeachae strain NCTC11477	450.86	wgs	PATRIC	450 tax ID	*
[Ref.: #66792]	Legionella longbeachae strain NCTC11477	450.87	wgs	PATRIC	450 tax ID	*
[Ref.: #66792]	Legionella longbeachae NCTC11477	GCA_004283175	scaffold	GenBank ENA	450 tax ID	*
[Ref.: #66792]	Legionella longbeachae strain FDAARGOS_1481	450.94	complete	PATRIC	450 tax ID	*
[Ref.: #66792]	Legionella longbeachae strain FDAARGOS_1481	450.95	complete	PATRIC	450 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella longbeachae strain FDAARGOS_1481 strain Not applicable PATRIC: 450.92
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	95.377	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	91.967	no
[Ref.: #125438]	aerobic	aerobicⓘ	no	72.253	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.494	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	56.184	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Legionella longbeachae strain FDAARGOS_1481 PATRIC: 450.94
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	96.8
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	51.5
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	97.5
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	97.9

Availability in culture collections External links

[Ref.: #3979]

Culture collection no.

CCUG 46622, CIP 103880, DSM 10572, ATCC 33462, NCTC 11477, Long Beach 4, CCUG 13335

[Ref.: #76199]

SI-ID 40509

Literature:

Topic	Title	Authors	Journal	DOI	Year
Stress	Resistance of Legionella and Acanthamoeba mauritaniensis to heat treatment as determined by relative and quantitative polymerase chain reactions.	Dobrowsky PH, Khan S, Khan W	Environ Res	10.1016/j.envres.2017.06.003	2017	*
Genetics	Sequence analysis of the mip gene of the soilborne pathogen Legionella longbeachae.	Doyle RM, Steele TW, McLennan AM, Parkinson IH, Manning PA, Heuzenroeder MW	Infect Immun	10.1128/IAI.66.4.1492-1499.1998	1998	*
Enzymology	Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia.	Lanser JA, Adams M, Doyle R, Sangster N, Steele TW	Appl Environ Microbiol	10.1128/aem.56.9.2784-2790.1990	1990	*
Pathogenicity	Legionella longbeachae species nova, another etiologic agent of human pneumonia.	McKinney RM, Porschen RK, Edelstein PH, Bissett ML, Harris PP, Bondell SP, Steigerwalt AG, Weaver RE, Ein ME, Lindquist DS, Kops RS, Brenner DJ	Ann Intern Med	10.7326/0003-4819-94-6-739	1981	*

References

#3979

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10572

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40846 ; Curators of the CIP;
#57290 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46622

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76199

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40509.1 )

#120647 Collection of Institut Pasteur ; Curators of the CIP; CIP 103880

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Legionella longbeachae strain FDAARGOS_1481 strain Not applicable

Legionella longbeachae strain FDAARGOS_1481

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