Strain identifier

BacDive ID: 6729

Type strain: Yes

Species: Legionella longbeachae

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.M. McKinney: strain Long Beach 4

NCBI tax ID(s): 450 (species)

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General

@ref: 3979

BacDive-ID: 6729

DSM-Number: 10572

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, rod-shaped

description: Legionella longbeachae CCUG 46622 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung.

NCBI tax id

  • NCBI tax id: 450
  • Matching level: species

strain history

@refhistory
3979<- H.E. Müller <- ATCC <- R. M. McKinney, strain Long Beach 4
120647CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.M. McKinney: strain Long Beach 4

doi: 10.13145/bacdive6729.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella longbeachae
  • full scientific name: Legionella longbeachae McKinney et al. 1982

@ref: 3979

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella longbeachae

full scientific name: Legionella longbeachae McKinney et al. 1982

type strain: yes

Morphology

cell morphology

  • @ref: 120647
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 3979
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3979BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40846MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120647CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
3979positivegrowth37mesophilic
40846positivegrowth37mesophilic
57290positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3979microaerophile
57290microaerophile
120647facultative anaerobe

enzymes

@refvalueactivityec
3979catalase+1.11.1.6
3979cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120647oxidase-
120647catalase+1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    57290C14:00.414
    57290C15:00.815
    57290C16:0416
    57290C17:00.617
    57290C14:0 ISO7.413.618
    57290C14:1 ω5c0.513.899
    57290C15:0 ANTEISO17.614.711
    57290C15:0 ISO0.314.621
    57290C15:1 ω6c2.214.856
    57290C16:0 iso23.315.626
    57290C16:1 ISO H0.315.461
    57290C16:1 ω5c2.715.908
    57290C16:1 ω7c23.715.819
    57290C17:0 anteiso5.316.722
    57290C17:0 CYCLO10.216.888
    57290C17:0 iso0.416.629
    57290C19:0 CYCLO ω9c0.518.87
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120647-+++++++--++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
3979human lungCaliforniaUSAUSANorth America
57290Human lung
120647Human, Lung

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Lung

taxonmaps

  • @ref: 69479
  • File name: preview.99_845.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_70;97_584;98_671;99_845&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: AY444740
  • Sequence Identity:
  • Total samples: 1856
  • soil counts: 787
  • aquatic counts: 754
  • animal counts: 213
  • plant counts: 102

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
39792Risk group (German classification)
1206472Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella longbeachae strain ATCC 33462 16S ribosomal RNA gene, partial sequenceAY4447401466ena450
20218Legionella longbeachae strain MDC1575 16S ribosomal RNA gene, partial sequenceJF720398524ena450
20218Legionella longbeachae 16S rRNA sequenceM360291473ena450

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella longbeachae strain FDAARGOS_1481450.92completepatric450
66792Legionella longbeachae strain NCTC11477450.86wgspatric450
66792Legionella longbeachae strain NCTC11477450.87wgspatric450
66792Legionella longbeachae NCTC11477GCA_004283175scaffoldncbi450
66792Legionella longbeachae strain FDAARGOS_1481450.94completepatric450
66792Legionella longbeachae strain FDAARGOS_1481450.95completepatric450
66792Legionella longbeachae strain FDAARGOS_1481450.93completepatric450

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.325no
anaerobicno98.348yes
halophileno89.776no
spore-formingno93.868no
glucose-utilyes68.006no
aerobicno89.395no
flagellatedno94.299no
thermophileno99.684yes
motileno73.478no
glucose-fermentno87.943no

External links

@ref: 3979

culture collection no.: CCUG 46622, CIP 103880, DSM 10572, ATCC 33462, NCTC 11477, Long Beach 4, CCUG 13335

straininfo link

  • @ref: 76199
  • straininfo: 40509

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1980403Genetic relatedness of Legionella longbeachae isolates from human and environmental sources in Australia.Lanser JA, Adams M, Doyle R, Sangster N, Steele TWAppl Environ Microbiol10.1128/aem.56.9.2784-2790.19901990Australia, Bacterial Typing Techniques, DNA Probes, Genetic Markers, Humans, Isoenzymes/genetics, Legionella/enzymology/genetics/*isolation & purification, Polymorphism, Restriction Fragment Length, SerotypingPathogenicity
Pathogenicity7235414Legionella longbeachae species nova, another etiologic agent of human pneumonia.McKinney RM, Porschen RK, Edelstein PH, Bissett ML, Harris PP, Bondell SP, Steigerwalt AG, Weaver RE, Ein ME, Lindquist DS, Kops RS, Brenner DJAnn Intern Med10.7326/0003-4819-94-6-7391981Aged, Female, Humans, Legionella/classification/*isolation & purification, Legionnaires' Disease/diagnosis/microbiology, Male, Middle Aged, Pneumonia/diagnosis/*etiology/microbiologyPhylogeny
Genetics9529072Sequence analysis of the mip gene of the soilborne pathogen Legionella longbeachae.Doyle RM, Steele TW, McLennan AM, Parkinson IH, Manning PA, Heuzenroeder MWInfect Immun10.1128/IAI.66.4.1492-1499.19981998Amino Acid Sequence, Animals, Bacterial Proteins/chemistry/*genetics/physiology, Base Sequence, *Genes, Bacterial, Genetic Complementation Test, Guinea Pigs, *Immunophilins, Legionella/*genetics/pathogenicity, Membrane Proteins/chemistry/*genetics/physiology, Molecular Sequence Data, *Peptidylprolyl Isomerase, Rabbits, *Soil Microbiology, Transcription, Genetic, VirulenceTranscriptome
Stress28609649Resistance of Legionella and Acanthamoeba mauritaniensis to heat treatment as determined by relative and quantitative polymerase chain reactions.Dobrowsky PH, Khan S, Khan WEnviron Res10.1016/j.envres.2017.06.0032017Acanthamoeba/genetics/microbiology/*physiology, *Hot Temperature, Legionella/genetics/*physiology, Real-Time Polymerase Chain Reaction

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3979Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10572)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10572
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40846Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15726
57290Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46622)https://www.ccug.se/strain?id=46622
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76199Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40509.1StrainInfo: A central database for resolving microbial strain identifiers
120647Curators of the CIPCollection of Institut Pasteur (CIP 103880)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103880