Limosilactobacillus mucosae (DSM 13345, CCUG 43179, CIP 106485)

Name: Limosilactobacillus mucosae (DSM 13345, CCUG 43179, CIP 106485)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6584

Type strain

Strain Designation S32

Culture col. no. DSM 13345 CCUG 43179 CIP 106485 S32 NCIMB 13705 3 more

NCBI tax ID(s) 1423771 97478

Special Collections DSMZ CCUG JCM KCTC CIP Pig DSMZ

History <- CCUG <- S. Roos; S32 CIP 106485 <-- S. Roos S32. CIP <- 2000, CCUG <- S. Roos, SLU, Swedish Univ. Agr. Sci., Uppsala, Sweden: strain S32

Links

Limosilactobacillus mucosae S32 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from pig small intestine.

Gram-positive rod-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13438

LMG 19534,CCUG 43179,CIP 106485,DSM 13345,NCIMB 13705,JCM 12515

@ref 20215

Last LPSN update 2026-02-09 11:19:15

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Limosilactobacillus

Species Limosilactobacillus mucosae

Full scientific name Limosilactobacillus mucosae (Roos et al. 2000) Zheng et al. 2020

Synonyms (1)

Lactobacillus mucosae

BacDive ID	Other strains from **Limosilactobacillus mucosae** (9)	Type strain
6585	L. mucosae S14, DSM 13346, CCUG 43180
159409	L. mucosae MRS-253-APC-2B, DSM 102820
160509	L. mucosae Cla-CZ-100, DSM 109049
165575	L. mucosae JCM 7717
165589	L. mucosae JCM 7735
165591	L. mucosae JCM 7737
173310	L. mucosae CRBIP24.8
175056	L. mucosae HUN087, AGR63, DSM 115836
175057	L. mucosae KHPX11, DSM 117469

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
121164	positive	rod-shaped
125438			90.5
125438	positive		95.436

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5007	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
39538	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
121164	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
5007	positive	growth	37
39538	positive	growth	37
55710	positive	growth	37
67770	positive	growth	37
121164	positive	growth	30-45
121164	negative	growth	10
121164	negative	growth	15
121164	negative	growth	22

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
55710	anaerobe
125439	microaerophile	95.1

Spore formation

@ref	Spore formation	Confidence
125438		92.27

Murein

@ref	Murein short key	Type
5007	A21.04	A4ß L-Orn-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
121164	17632 ChEBI	nitrate	-	reduction
121164	17632 ChEBI	nitrate	+	respiration
121164	16301 ChEBI	nitrite	-	reduction
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
121164	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121164	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
121164	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
121164	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
121164	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121164	ornithine decarboxylase	-	4.1.1.17
121164	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121164	-	-	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
5007	-	-	-	-	-	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.
5007	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
5007	-	-	-	-	-	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Gastrointestinal tract	#Small intestine

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
5007	pig small intestine			Sweden	SWE	Europe
55710	Pig small intestine,newly slaughtered	1997-09-01	Uppsala	Sweden	SWE	Europe
67770	Small intestine of pig
121164	Animal, Pig, small intestine		Uppsala	Sweden	SWE	Europe	1997

Taxonmaps

69479

Global distribution of 16S sequence LC383823 (>99% sequence identity) for Limosilactobacillus mucosae subclade from Microbeatlas

Aquatic Samples	7206
Soil Samples	2576
Animal Samples	94221
Plant Samples	1502
Total Samples	105505

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
5007	1	Risk group (German classification) According to TRBA 466
121164	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM4338670v1 assembly for Limosilactobacillus mucosae DSM 13345	chromosome	GCA_043386705			97478	87.02
67770	ASM143602v1 assembly for Limosilactobacillus mucosae DSM 13345	contig	GCA_001436025	1423771.3	2657245411	1423771	61.35

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
5007	Lactobacillus mucosae 16S ribosomal RNA gene, complete sequence	AF126738	1568	97478
67770	Lactobacillus mucosae JCM 12515 gene for 16S ribosomal RNA, partial sequence	LC383823	1515	97478
124043	Lactobacillus mucosae gene for 16S rRNA, partial sequence, strain: JCM 12515.	AB289204	636	97478

GC content

@ref	GC-content (mol%)	Method
5007	46	high performance liquid chromatography (HPLC)
67770	46.3-46.7	high performance liquid chromatography (HPLC)
67770	46.4	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Limosilactobacillus mucosae DSM 13345
NCBI: GCA_001436025

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	80.10	no
125439	motility	BacteriaNetⓘ	no	54.40	no
125439	gram_stain	BacteriaNetⓘ	positive	78.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	95.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus mucosae DSM 13345
PATRIC: 1423771.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	95.44	no
125438	anaerobic	anaerobicⓘ	no	73.39	yes
125438	spore-forming	spore-formingⓘ	no	92.27	no
125438	aerobic	aerobicⓘ	no	94.45	no
125438	thermophilic	thermophileⓘ	no	95.00	yes
125438	flagellated	motile2+ⓘ	no	90.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic.	Kavanova K, Kostovova I, Moravkova M, Kubasova T, Babak V, Crhanova M.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10259-7	2025
Genetics	Screening, Identification, and Whole-Genome Sequencing of Ferulic Acid Esterase-Producing Lactic Acid Bacteria from Sheep Rumen.	Qiu M, Chen Y, Wang L, Li L, Zhang X, Ma Z, Liu J.	Microorganisms	10.3390/microorganisms13061295	2025
Metabolism	Faecal D/L lactate ratio is a metabolic signature of microbiota imbalance in patients with short bowel syndrome.	Mayeur C, Gratadoux JJ, Bridonneau C, Chegdani F, Larroque B, Kapel N, Corcos O, Thomas M, Joly F.	PLoS One	10.1371/journal.pone.0054335	2013
Enzymology	Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture.	Starke IC, Zentek J, Vahjen W	Benef Microbes	10.3920/BM2014.0052	2015
Phylogeny	Development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces.	Selim AS, Boonkumklao P, Sone T, Assavanig A, Wada M, Yokota A	Appl Environ Microbiol	10.1128/AEM.71.8.4214-4219.2005	2005

References

#5007	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13345
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#39538	; Curators of the CIP;
#55710	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43179
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121164	Collection of Institut Pasteur ; Curators of the CIP; CIP 106485
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Limosilactobacillus mucosae (DSM 13345, CCUG 43179, CIP 106485)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Murein

Metabolite utilization

Antibiotic resistance

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_043386705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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